Consulta: creatorFacets:"Colliec-jouault, Sylvia"
Registros recuperados: 47
Data/hora: 07/06/2024 12:26:07

Effect of low-molecular-weight fucoidan on experimental arterial thrombosis in the rabbit and rat
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Colliec-jouault, Sylvia; Millet, J; Helley, D; Sinquin, Corinne; Fischer, Am.
Tipo: Text
Palavras-chave: Arterial thrombosis; Polyaccharide; Fucoidan.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-2116.pdf

Design Polysaccharides of Marine Origin: Chemical Modifications to Reach Advanced Versatile Compounds
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Chopin, Nathalie; Guillory, Xavier; Weiss, Pierre; Le Bideau, Jean; Colliec-jouault, Sylvia.
Polysaccharides are among the most abundant macromolecules on Earth. These polymers are easily obtained from various marine resources such as algae, microorganisms and crustacean shells. The structure of these natural carbohydrates is innovative and quite complex. Marine biopolymers represent key scaffolds toward large challenging fields, such as biomedical applications (glycosaminoglycans, regenerative medicine and drug delivery) and tailored biomaterials. Chemical modifications can be applied to modify their final properties in a specific purpose. New functional glycans are achievable and represent a real potential with their intrinsic biocompatibility and biodegradability. Hydroxyl groups are ubiquitous in polysaccharides structure and involved in most...
Tipo: Text
Palavras-chave: Alginates; Carrageenans; Chemical modification; Chitin; Chitosan; Grafting; Marine polysaccharide; Polymer.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00199/31027/29902.pdf

Effects of a sulfated exopolysaccharide produced by Altermonas infernus on bone biology
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Velasco, C. Ruiz; Baud'Huin, M.; Sinquin, Corinne; Maillasson, M.; Heymann, D.; Colliec-jouault, Sylvia; Padrines, M.
The growth and differentiation of bone cells is controlled by various factors which can be modulated by heparan sulphates. Here, we investigated the effects of an oversulphated exopolysaccharide (OS-EPS) on bone. We compared the effect of this compound with that of a native exopolysaccharide (EPS). Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulphation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the...
Tipo: Text
Palavras-chave: Bone metabolism; Bone remodeling; Exopolysaccharide; Glycosaminoglycan; Heparin.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00032/14300/15749.pdf

Exopolysaccharide biosynthesis and biodegradation by a marine hydrothermal Alteromonas sp. strain
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lelchat, Florian; Cozien, Joelle; Le Costaouec, Tinaig; Brandily, Christophe; Schmitt, Sophie; Baudoux, Anne-claire; Colliec-jouault, Sylvia; Boisset, Claire.
Alteromonas macleodii subsp. fijiensis biovar deepsane is a deep-sea ecotype exopolysaccharide-producing bacteria isolated from the polychaete annelid Alvinella pompejana. The high molecular weight biopolymer HYD657 produced by this strain, is the first marine exopolysaccharide (EPS) to be commercialized for cosmetic use. Depolymerization methods are necessary to elucidate the complete structure of this EPS and to generate potentially bioactive oligosaccharides. Enzymatic methods are useful for elucidating polysaccharide structure because they specifically cleave glycosidic bonds and do not require harsh chemical conditions. The HYD657 EPS is structurally complex and no commercially available enzymes are able to effectively degrade it. Here, we present the...
Tipo: Text
Palavras-chave: Marine Alteromonas macleodii sp strain; Bacterial exopolysaccharides; Microbial extracellular enzymes; Endogenous enzymatic degradation.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00240/35129/33893.pdf

Ultrasonic depolymerization of an exopolysaccharide produced by a bacterium isolated from a deep-sea hydrothermal vent polychaete annelid
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Petit, Anne-celine; Noiret, Nicolas; Guezennec, Jean; Gondrexon, N; Colliec-jouault, Sylvia.
Low frequency ultrasound was used to depolymerize a high-molecular-weight exopolysaccharide (EPS) produced by a deep-sea hydrothermal bacterium Alteromonas macleodii subsp. fijiensis biovar deepsane. The influence of several parameters was examined including the duration of ultrasonic irradiation, EPS concentration. reaction temperature and volume of the sonicated solution. With the aim of optimizing the depolyrnerization, the native EPS was simultaneously treated with hydrogen peroxide and ultrasound. This study identified the sonication conditions that produce low-molecular-weight derivatives from the native EPS (&gt;10(6) Da) with good reproducibility. (C) 2006 Elsevier B.V. All rights reserved.
Tipo: Text
Palavras-chave: Exopolysaccharide; Depolymerization; Ultrasound.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2123.pdf

Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M.
To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is...
Tipo: Text
Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf

Free-radical depolymerization with metallic catalysts of an exopolysaccharide produced by a bacterium isolated from a deep-sea hydrothermal vent polychaete annelid
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Petit, Anne-celine; Noiret, N; Sinquin, Corinne; Ratiskol, Jacqueline; Guezennec, Jean; Colliec-jouault, Sylvia.
Free-radical depolymerization with metallic catalysts has been investigated to depolymerize an exopolysaccharide (EPS), with a high molecular weight (&gt; 10(6) g/Mol), produced by the bacterium Alteromonas macleodii subsp. fijiensis biovar deepsane. Three metals (copper, zinc and manganese) were used either alone or mixed. These metals led to the depolymerization of the high molecular weight bacterial polysaccharide with different efficiency. This study specifies some conditions required to produce, with good reproducibility and yield, EPS derivatives of reduced molecular weight ranging from 100,000 to 20,000 g/mol. The more influential parameters are specified. This is a first study to find suitable industrial conditions in the presence of these metallic...
Tipo: Text
Palavras-chave: Bacterial exopolysaccharide; Free radical depolymerization; H202; Metal.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2146.pdf

Enriching a cellulose hydrogel with a biologically active marine exopolysaccharide for cell-based cartilage engineering
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rederstorff, E.; Rethore, G.; Weiss, P.; Sourice, S.; Beck-cormier, S.; Mathieu, E.; Maillasson, M.; Jacques, Y.; Colliec-jouault, Sylvia; Fellah, B. H.; Guicheux, J.; Vinatier, C.
The development of biologically and mechanically competent hydrogels is a prerequisite in cartilage engineering. We recently demonstrated that a marine exopolysaccharide, GY785, stimulates the in vitro chondrogenesis of adipose stromal cells. In the present study, we thus hypothesized that enriching our silated hydroxypropyl methylcellulose hydrogel (Si-HPMC) with GY785 might offer new prospects in the development of scaffolds for cartilage regeneration. The interaction properties of GY785 with growth factors was tested by surface plasmon resonance (SPR). The biocompatibility of Si-HPMC/GY785 towards rabbit articular chondrocytes (RACs) and its ability to maintain and recover a chondrocytic phenotype were then evaluated in vitro by MTS assay, cell counting...
Tipo: Text
Palavras-chave: Cartilage; Hydrogel; Glycosaminoglycan; Marine polysaccharides; Chondrocytes; Tissue engineering.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00303/41449/40650.pdf

Low-molecular-weight fucoidan enhances the proangiogenic phenotype of endothelial progenitor cells
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zemani, Faouzia; Benisvy, Danielle; Galy Fauroux, Isabelle; Lokajczyk, Anna; Colliec-jouault, Sylvia; Uzan, Georges; Fischer, Anne Marie; Boisson Vidal, Catherine.
Endothelial progenitor cell (EPC) transplantation is a potential means of inducing neovascularization in vivo. However, the number of circulating EPC is relatively small, it may thus be necessary to enhance their proangiogenic properties ex vivo prior to injection in vivo. Fucoidan has previously been shown to potentiate in vitro tube formation by mature endothelial cells in the presence of basic fibroblast growth factor (FGF-2). We therefore examined whether fucoidan, alone or combined with FGF-2, could increase EPC proangiogenic potency in vitro. EPC exposure to 10 mu g/ml fucoidan induced a proangiogenic phenotype, including cell proliferation (p &lt; 0.01) and migration (p &lt; 0.01); moreover, differentiation into vascular cords occurred in the...
Tipo: Text
Palavras-chave: Fucoidan; FGF 2; Endothelial progenitor cells; Angiogenesis; Alpha 6.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-679.pdf

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zykwinska, Agata; Marquis, Melanie; Godin, Mathilde; Marchand, Laetitia; Sinquin, Corinne; Garnier, Catherine; Jonchere, Camille; Chedeville, Claire; Le Visage, Catherine; Guicheux, Jerome; Colliec-jouault, Sylvia; Cuenot, Stephane.
Articular cartilage is an avascular, non-innervated connective tissue with limited ability to regenerate. Articular degenerative processes arising from trauma, inflammation or due to aging are thus irreversible and may induce the loss of the joint function. To repair cartilaginous defects, tissue engineering approaches are under intense development. Association of cells and signalling proteins, such as growth factors, with biocompatible hydrogel matrix may lead to the regeneration of the healthy tissue. One current strategy to enhance both growth factor bioactivity and bioavailability is based on the delivery of these signalling proteins in microcarriers. In this context, the aim of the present study was to develop microcarriers by encapsulating...
Tipo: Text
Palavras-chave: Exopolysaccharide; Growth factor; Microparticles; Microfluidics; Bioactivity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00477/58882/61432.pdf

Heparin-like Entities from Marine Organisms
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Colliec-jouault, Sylvia; Bavington, C.; Delbarre-ladrat, Christine.
Polysaccharides are ubiquitous in animals and plant cells where they play a significant role in a number of physiological situations e.g. hydration, mechanical properties of cell walls and ionic regulation. This review concentrates on heparin-like entities from marine procaryotes and eukaryotes. Carbohydrates from marine prokaryotes offer a significant structural chemodiversity with novel material and biological properties. Cyanobacteria are Gram-negative photosynthetic prokaryotes considered as a rich source of novel molecules, and marine bacteria are a rich source of polysaccharides with novel structures, which may be a good starting point from which to synthesise heparinoid molecules. For example, some sulphated polysaccharides have been isolated from...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00127/23874/21850.pdf

Viral degradation of marine bacterial exopolysaccharides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lelchat, Florian; Mocaer, P Y; Ojima, T; Michel, G; Sarthou, Geraldine; Bucciarelli, Eva; Cérantola, S; Colliec-jouault, Sylvia; Boisset, Claire; Baudoux, A-c.
The identification of the mechanisms by which marine dissolved organic matter (DOM) is produced and regenerated is critical to develop robust prediction of ocean carbon cycling. Polysaccharides represent one of the main constituents of marine DOM and their degradation is mainly attributed to polysaccharidases derived from bacteria. Here, we report that marine viruses can depolymerize the exopolysaccharides (EPS) excreted by their hosts using 5 bacteriophages that infect the notable EPS producer, Cobetia marina DSMZ 4741. Degradation monitorings as assessed by gel electrophoresis and size exclusion chromatography showed that 4 out of 5 phages carry structural enzymes that depolymerize purified solution of Cobetia marina EPS. The depolymerization patterns...
Tipo: Text
Palavras-chave: Marine phage; EPS; Polysaccharidase; DOM; Ocean.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00499/61062/64512.pdf

Neoangiogenesis Induced by Progenitor Endothelial Cells: Effect of Fucoidan from Marine Algae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boisson-vidal, C; Zemani, F; Calliguiri, G; Galy-fauroux, I; Colliec-jouault, Sylvia; Helley, D; Fischer, A-m.
Fucoidans -- sulphated polysaccharides extracted from brown algae - could be beneficial in patients with ischemic diseases. Their antithrombotic and proangiogenic properties promote in animals, neovascularization and angiogenesis which prevent necrosis of ischemic tissue. In 1997, endothelial progenitor cells were first identified in human peripheral blood. They are recruited from bone marrow and contribute to neovascularization after ischemic injury. Mobilization of these cells in ischemic sites is an important step in new vessel formation. It is thought that the progenitors interact with endothelial cells, then extravasate and reach ischemic sites, where they proliferate and differentiate into new blood vessels. Although chemokines, cytokines and...
Tipo: Text
Palavras-chave: Growth factors; Cytokines; Angiogenesis; Neovascularization; Endothelial progenitor cells; Sulphated polysaccharides; Fucoidan.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2168.pdf

Effect of Low Molecular Weight Fucoidan and Low Molecular Weight Heparin in a Rabbit Model of Arterial Thrombosis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Durand, E; Helley, D; Zen, A; Dujols, C; Bruneval, P; Colliec-jouault, Sylvia; Fischer, Aurelie; Lafont, A.
Background: Therapeutic use of unfractionated heparin and low molecular weight heparins (LMWHs) is limited by hemorrhagic adverse effects. We compared the antithrombotic effect of LMW fucoidan (LMWF) and LMWH in an experimental model. Methods: Thrombosis was induced in femoral arteries of male New Zealand White rabbits by in situ induction of endothelial apoptosis with staurosporine (10(-5) M for 30 min). Starting the day before apoptosis induction, the animals received subcutaneous LMWF (15 mg/kg), LMWH (enoxaparin 2.5 mg/kg) or saline solution (control group) twice a day for 4 days. Results: The degrees of apoptosis and endothelial denudation were similar in the 3 groups. The thrombotic score was significantly lower in the LMWF group than in the LMWH and...
Tipo: Text
Palavras-chave: Tissue factor; Tissue factor pathway inhibitor; Heparin; Fucoidan; Thrombosis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4744.pdf

Antiviral Activities of Sulfated Polysaccharides Isolated from Sphaerococcus coronopifolius (Rhodophytha, Gigartinales) and Boergeseniella thuyoides (Rhodophyta, Ceramiales)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bouhlal, Rhimou; Haslin, Camille; Chermann, Jean-claude; Colliec-jouault, Sylvia; Sinquin, Corinne; Simon, Gaelle; Cerantola, Stephane; Riadi, Hassane; Bourgougnon, Nathalie.
Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 mu g/mL. In addition, polysaccharides were capable of inhibiting the in vitro replication of Herpes simplex virus type 1 (HSV-1) on Vero cells values of EC(50) of 4.1 and 17.2 mu g/mL, respectively. The adsorption step of HSV-1 to the host cell seems to be the specific target for polysaccharide action. While for HIV-1, these results suggest a direct inhibitory effect on HIV-1 replication by controlling the appearance of the new generations of virus and...
Tipo: Text
Palavras-chave: Herpes simplex virus; Human immunodeficiency virus; Antiviral activity; Sulfated polysaccharide; S. coronopifolius; B. thuyoides; Chemical composition.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00060/17120/14631.pdf

Therapeutic effect of fucoidan-stimulated endothelial colony-forming cells in peripheral ischemia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Sarlon, G.; Zemani, F.; David, L.; Van Huyen, J. -p. Duong; Dizier, B.; Grelac, F.; Colliec-jouault, Sylvia; Galy-fauroux, Isabelle; Bruneval, P.; Fischer, Anne-marie; Emmerich, J.; Boisson-vidal, Catherine.
. Background: Fucoidan, an antithrombotic polysaccharide, can induce endothelial colony-forming cells (ECFC) to adopt an angiogenic phenotype in vitro. Objectives: We evaluated the effect of fucoidan on vasculogenesis induced by ECFC in vivo. Methods: We used a murine hindlimb ischemia model to probe the synergic role of fucoidan-treatment and ECFC infusion during tissue repair. Results: We found that exposure of ECFC to fucoidan prior to their intravenous injection improved residual muscle blood flow and increased collateral vessel formation. Necrosis of ischemic tissue was significantly reduced on day 14, to 12.1% of the gastronecmius cross-sectional surface area compared with 40.1% in animals injected with untreated-ECFC. ECFC stimulation with fucoidan...
Tipo: Text
Palavras-chave: Angiogenesis; Endothelial colony-forming cells; Fucoidan; Ischemia; Revascularization.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00065/17616/18204.pdf

Structural studies of the main exopolysaccharide produced by the deep-sea bacterium Alteromonas infernus
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Roger, Olivier; Kervarec, Nelly; Ratiskol, Jacqueline; Colliec-jouault, Sylvia; Chevolot, Lionel.
The structure of the extracellular polysaccharide produced by the mesophilic species, Alteromonas infernus, found in deep-sea hydrothermal vents and grown under laboratory conditions, has been investigated using partial depolymerization, methylation analysis, mass spectrometry and NMR spectroscopy. The repeating units of this polysaccharide is a nonasaccharide with the following structure:   [GRAPHICS]   (C) 2004 Elsevier Ltd. All rights reserved.
Tipo: Text
Palavras-chave: Hydrothermal vent; Structure; Bacterial polysaccharide; Alteromonas.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2112.pdf

Bioprospecting for Exopolysaccharides from Deep-Sea Hydrothermal Vent Bacteria: Relationship between Bacterial Diversity and Chemical Diversity
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Delbarre-ladrat, Christine; Salas, Marcia Leyva; Sinquin, Corinne; Zykwinska, Agata; Colliec-jouault, Sylvia.
Many bacteria biosynthesize structurally diverse exopolysaccharides (EPS) and excrete them into their surrounding environment. The EPS functional features have found many applications in industries such as cosmetics and pharmaceutics. In particular, some EPS produced by marine bacteria are composed of uronic acids, neutral sugars, and N-acetylhexosamines, and may also bear some functional sulfate groups. This suggests that they can share common structural features with glycosaminoglycans (GAG) like the two EPS (HE800 and GY785) originating from the deep sea. In an attempt to discover new EPS that may be promising candidates as GAG-mimetics, fifty-one marine bacterial strains originating from deep-sea hydrothermal vents were screened. The analysis of the...
Tipo: Text
Palavras-chave: Exopolysaccharides; GAG; Marine bacteria; Glycopolymers; Sulfate; Production.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00399/51090/51873.pdf

Sterilization of Exopolysaccharides Produced by Deep-Sea Bacteria: Impact on Their Stability and Degradation
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rederstorff, Emilie; Fatimi, Ahmed; Sinquin, Corinne; Ratiskol, Jacqueline; Merceron, Christophe; Vinatier, Claire; Weiss, Pierre; Colliec-jouault, Sylvia.
Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared...
Tipo: Text
Palavras-chave: Marine biotechnology; Polysaccharides; Sterilization procedures; Characterization; Molecular weight distribution; Rheology.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00027/13776/10913.pdf

Low-Molecular-Weight Fucoidan Induces Endothelial Cell Migration via the PI3K/AKT Pathway and Modulates the Transcription of Genes Involved in Angiogenesis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bouvard, Claire; Galy-fauroux, Isabelle; Grelac, Francoise; Carpentier, Wassila; Lokajczyk, Anna; Gandrille, Sophie; Colliec-jouault, Sylvia; Fischer, Anne-marie; Helley, Dominique.
Low-molecular-weight fucoidan (LMWF) is a sulfated polysaccharide extracted from brown seaweed that presents antithrombotic and pro-angiogenic properties. However, its mechanism of action is not well-characterized. Here, we studied the effects of LMWF on cell signaling and whole genome expression in human umbilical vein endothelial cells and endothelial colony forming cells. We observed that LMWF and vascular endothelial growth factor had synergistic effects on cell signaling, and more interestingly that LMWF by itself, in the absence of other growth factors, was able to trigger the activation of the PI3K/AKT pathway, which plays a crucial role in angiogenesis and vasculogenesis. We also observed that the effects of LMWF on cell migration were...
Tipo: Text
Palavras-chave: Fucoidan; Angiogenesis; Vasculogenesis; Migration; Signaling; Transcriptomics.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00308/41913/41187.pdf

Pharmacological Modulation of Human Mesenchymal Stem Cell Chondrogenesis by a Chemically Oversulfated Polysaccharide of Marine Origin: Potential Application to Cartilage Regenerative Medicine
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Merceron, Christophe; Portron, Sophie; Vignes-colombeix, Caroline; Rederstorff, Emilie; Masson, Martial; Lesoeur, Julie; Sourice, Sophie; Sinquin, Corinne; Colliec-jouault, Sylvia; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome.
Mesenchymal stem cells (MSCs) are considered as an attractive source of cells for cartilage engineering due to their availability and capacity for expansion and multipotency. Differentiation of MSC into chondrocytes is crucial to successful cartilage regeneration and can be induced by various biological agents, including polysaccharides that participate in many biological processes through interactions with growth factors. Here, we hypothesize that growth factor-induced differentiation of MSC can be increased by chemically oversulfated marine polysaccharides. To test our hypothesis, human adipose tissue-derived MSCs (hATSCs) were cultured in pellets with transforming growth factor (TGF)-beta 1-supplemented chondrogenic medium containing either the...
Tipo: Text
Palavras-chave: Cartilage; Adipose-derived mesenchymal stem cells; Polysaccharides; Transforming growth factor-beta.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00071/18227/17457.pdf

Fucoidan a sulfated polysaccharide from brown algae is a potent modulator of connective tissue proteolysis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Senni, Karim; Gueniche, Farida; Foucault Bertaud, Alexandrine; Igondjo Tchen, Sylvie; Fioretti, Florence; Colliec-jouault, Sylvia; Durand, Patrick; Guezennec, Jean; Godeau, Gaston; Letourneur, Didier.
Fucoidans are sulfated fucosylated polymers from brown algae cell wall that exhibit some heparin/heparan sulfate properties. We previously demonstrated that these polysaccharides were able in vitro to stimulate dermal fibroblast proliferation and extracellular matrix deposition. Here, we investigated the action of a 16 kDa fucoidan fraction on parameters involved in connective tissue breakdown. This fucoidan is able to inhibit gelatinase A secretion and stromelysin 1 induction by interleukin-1 beta on dermal fibroblasts in culture. Furthermore, we observed that fucoidan increases the rate of association of MMPs with their specific inhibitors namely TIMPs. Using tissue sections of human skin in ex vivo experiments, we evidenced that this polysaccharide was...
Tipo: Text
Palavras-chave: Interleukin 1 beta; Leukocyte elastase; Matrix metalloproteinases; Extracellular matrix remodeling; Glycosaminoglycans; Fucoidan.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2147.pdf

The Use of Natural Polysaccharides as Biomaterials
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Laurienzo, Paola; Fernandes, Joao C.; Colliec-jouault, Sylvia; Fitton, J. Helen.
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00271/38259/36442.pdf

Enzymatic depolymerization of the GY785 exopolysaccharide produced by the deep-sea hydrothermal bacterium Alteromonas infernus : structural study and enzyme activity assessment
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zykwinska, Agata; Berre, Ludivine Tripon-le; Sinquin, Corinne; Ropartz, David; Rogniaux, Helene; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened...
Tipo: Text
Palavras-chave: Exopolysaccharide; Enzyme; Depolymerization; Oligosaccharide; Mass spectrometry.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00423/53458/54352.pdf

Antiproliferative Properties of Scandium Exopolysaccharide Complexes on Several Cancer Cell Lines
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Muñoz-garcia, Javier; Mazza, Mattia; Alliot, Cyrille; Sinquin, Corinne; Colliec-jouault, Sylvia; Heymann, Dominique; Huclier-markai, Sandrine.
Antimetastatic properties on both murine and human osteosarcoma cell lines (POS-1 and KHOS) have been evidenced using exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium. These derivatives had no significant effect on the cell cycle neither a pro-apoptotic effect on osteosarcoma cells. Based on this observation, these EPSs could be employed as new drug delivery systems for therapeutic uses. A theranostic approach, i.e., combination of a predictive biomarker with a therapeutic agent, has been developed notably by combining with true pair of theranostic radionuclides, such as scandium 47Sc/44Sc. However, it is crucial to ensure that, once complexation is done, the biological properties of the vector remain intact, allowing the...
Tipo: Text
Palavras-chave: Exopolysaccharides; Scandium; Theranostic; Cancer cell lines; Proliferation.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00686/79826/82630.pdf

An in vitro study of two GAG-like marine polysaccharides incorporated into injectable hydrogels for bone and cartilage tissue engineering
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rederstorff, Emilie; Weiss, Pierre; Sourice, S.; Pilet, P.; Xie, F.; Sinquin, Corinne; Colliec-jouault, Sylvia; Guicheux, Jerome; Laib, S.
Natural polysaccharides are attractive compounds with which to build scaffolds for bone and cartilage tissue engineering. Here we tested two non-standard ones, HE800 and GY785, for the two-dimensional (2-D) and three-dimensional (3-D) culture of osteoblasts (MC3T3-E1) and chondrocytes (C28/I2). These two glycosaminoglycan-like marine exopolysaccharides were incorporated into an injectable silylated hydroxypropylmethylcellulose-based hydrogel (Si-HPMC) that has already shown its suitability for bone and cartilage tissue engineering. Results showed that, similarly to hyaluronic acid (HA) (the control), HE800 and GY785 significantly improved the mechanical properties of the Si-HPMC hydrogel and induced the attachment of MC3T3-E1 and C28/I2 cells when these...
Tipo: Text
Palavras-chave: Glycosaminoglycan; Hydrogel; Polysaccharide; Bone and cartilage tissue engineering; In vitro test.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00030/14090/15747.pdf

Screening of enzymatic activities for the depolymerisation of the marine bacterial exopolysaccharide HE800
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rigouin, Coraline; Delbarre-ladrat, Christine; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, Michel.
Tipo: Text
Palavras-chave: Polysaccharide; Enzymatic depolymerisation; Low-molecular-weight derivatives; Enterococcus faecalis; Endo-N-acetyl-galactosaminidase.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20907/18741.pdf

Low molecular weight fucoidan promotes FGF-2-induced vascular tube formation by human endothelial cells, with decreased PAI-1 release and ICAM-1 downregulation
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Chabut, D; Fischer, A; Helley, D; Colliec-jouault, Sylvia.
Tipo: Text
Palavras-chave: Fucoidan; Vascularization; Endothelial cell; Human therapy.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2115.pdf

Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zykwinska, Agata; Marchand, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment. The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS). EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve. Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups. Such a particular composition ensures interesting functional properties, including biological activities mimicking...
Tipo: Text
Palavras-chave: Exopolysaccharide; Glycosaminoglycan; Uronic acid; Hexosamine; Phylogenetic analysis.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00506/61756/65728.pdf

Capillary electrophoresis determination of the binding affinity of bioactive sulfated polysaccharides to proteins: study of the binding properties of fucoidan to antithrombin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Varenne, A; Gareil, P; Colliec-jouault, Sylvia; Daniel, R.
The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. This method is a variant of zonal electrophoresis in the mobility shift format. A fixed amount of protein was injected into a capillary filled with a background electrolyte containing the polysaccharide in varying concentrations. The effective mobility data of the protein were processed according to classical linearization treatments to...
Tipo: Text
Palavras-chave: Antithrombin; Fucoidan; Electrophoresis; Protein interaction; Polysaccharides.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-2114.pdf

Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Norris, Karl; Mishukova, Oksana; Zykwinska, Agata; Colliec-jouault, Sylvia; Sinquin, Corinne; Koptioug, Andrei; Cuenot, Stéphane; Kerns, Jemma; Surmeneva, Maria; Surmenev, Roman; Douglas, Timothy.
Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of...
Tipo: Text
Palavras-chave: Marine exopolysaccharide; Collagen; Surface modification; Ti6Al4V.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00477/58881/61433.pdf

Exopolysaccharides produced by marine bacteria and their applications as glycosaminoglycan-like molecules
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Delbarre-ladrat, Christine; Sinquin, Corinne; Lebellenger, Lou; Zykwinska, Agata; Colliec-jouault, Sylvia.
Although polysaccharides are ubiquitous and the most abundant renewable bio-components, their studies, covered by the glycochemistry and glycobiology fields, remain a challenge due to their high molecular diversity and complexity. Polysaccharides are industrially used in food products; human therapeutics fall into a more recent research field and pharmaceutical industry is looking for more and more molecules with enhanced activities. Glycosaminoglycans (GAGs) found in animal tissues play a critical role in cellular physiological and pathological processes as they bind many cellular components. Therefore, they present a great potential for the design and preparation of therapeutic drugs. On the other hand, microorganisms producing exopolysaccharides (EPS)...
Tipo: Text
Palavras-chave: Marine-derivedpolysaccharide; Biodiversity; Biologicalactivity; Structure; Modification; GAG-mimetic; Biosynthesis.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00241/35250/33764.pdf

Marine Polysaccharides: A Source of Bioactive Molecules for Cell Therapy and Tissue Engineering
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia.
The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the...
Tipo: Text
Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf

Les sucres de l'extrême pour la médecine de demain
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Colliec-jouault, Sylvia.
À la fin des années 1980, l'Ifremer a initié des recherches sur les micro-organismes des écosystèmes hydrothermaux océaniques profonds. Parmi les échantillons prélevés – environ 1330 isolats –, des bactéries productrices de biopolymères d’intérêt biotechnologique, comme des exopolysaccharides (EPS) et des polyhydroxyalcanaotes (PHA), ont été identifiées. De genres connus, elles appartiennent à des espèces ignorées jusqu’alors (Alteromonas infernus, Alteromonas macleodii subsp. fijiensis biovar deepsane ou Vibrio diabolicus).
Tipo: Text
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00252/36325/34887.pdf

Marine Exopolysaccharide Complexed with Scandium Aimed as Theranostic Agents
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mazza, Mattia; Alliot, Cyrille; Sinquin, Corinne; Colliec-jouault, Sylvia; Reiller, Pascal E.; Huclier-markai, Sandrine.
Background: Exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium, showed anti-metastatic properties. They may represent a new class of ligands to be combined with theranostic radionuclides, such as 47Sc/44Sc. The goal of this work was to investigate the feasibility of such coupling. (2) Methods: EPSs, as well as heparin used as a drug reference, were characterized in terms of molar mass and dispersity using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering (AF4-MALS). The intrinsic viscosity of EPSs at different ionic strengths were measured in order to establish the conformation. To determine the stability constants of Sc with EPS and heparin, a Free-ion selective radiotracer extraction (FISRE)...
Tipo: Text
Palavras-chave: Exopolysaccharides; Scandium; Theranostic; Characterization; Complexation.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00681/79332/81844.pdf

Potential effects of a low molecular weight Fucoidan extracted from brown algae on bone biomaterial osteoconductive properties
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Igondjo Tchen Changotade, S; Korb, G; Bassil, J; Barroukh, B; Willig, C; Colliec-jouault, Sylvia; Durand, Patrick; Godeau, G; Senni, K.
In this work we firstly tested the influence of low molecular weight fucoidan extracted from pheophicae cell wall on bidimensional cultured normal human osteoblasts behaviours. Secondly impregnation procedure with LMW fucoidan of bone biomaterial (Lubboc®) we explored in this bone extracellular matrix context its capabilities to support human osteoblastic behaviour in 3D culture. In bidimensionnal cultures we evidenced that: LMW fucoidan promotes human osteoblast proliferation, collagen type I expression and favours precocious alkaline phosphatase activity. Furthermore with LMW fucoidan von Kossa's staining was positive at 30 days and only positive at 45 days in absence of LMW fucoidan. In our three dimensional culture models with the biomaterial...
Tipo: Text
Palavras-chave: Heparan mimetics; Biomaterial; Bone; Osteoblast; Fucoidan.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2007/publication-2695.pdf

Osteoprotegerin, a new actor in vasculogenesis, stimulates endothelial colony-forming cells properties
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Benslimane-ahmim, Z.; Heymann, D.; Dizier, B.; Lokajczyk, A.; Brion, R.; Laurendeau, I.; Bieche, I.; Smadja, D. M.; Galy-fauroux, I.; Colliec-jouault, Sylvia; Fischer, Anne-marie; Boisson-vidal, Catherine.
Background: Osteoprotegerin (OPG), a soluble receptor of the tumour necrosis factor family, and its ligand, the receptor activator of nuclear factor-kappa B ligand (RANKL), are emerging as important regulators of vascular pathophysiology. Objectives: We evaluated their effects on vasculogenesis induced by endothelial colony-forming cells (ECFC) and on neovessel formation in vivo. Methods: Effects of OPG and RANKL on in vitro angiogenesis were evaluated after ECFC incubation with OPG or RANKL (0-50 ng mL(-1)). Effects on microvessel formation were evaluated with an in vivo murin Matrigel plug assay. Vascularization was evaluated by measuring plug hemoglobin and vascular endothelial growth factor (VEGF)-R2 content 14 days after implantation. Results: We...
Tipo: Text
Palavras-chave: Endothelial colony-forming cells; Osteoprotegerin; Receptor activator of nuclear factor-kappa B ligand; Vasculogenesis.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14462/11863.pdf

Assembly of HE800 exopolysaccharide produced by a deep-sea hydrothermal bacterium into microgels for protein delivery applications
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zykwinska, Agata; Marquis, Melanie; Sinquin, Corinne; Cuenot, Stephane; Colliec-jouault, Sylvia.
Assembly of biopolymers into microgels is an elegant strategy for bioencapsulation with various potential biomedical applications. Such biocompatible and biodegradable microassemblies are developed not only to protect the encapsulated molecule but also to ensure its sustained local delivery. The present study describes the fabrication of microassemblies from a marine HE800 exopolysaccharide (EPS), which displays a glycosaminoglycan (GAG)-like structure and biological properties. HE800 EPS was assembled, through physical cross-linking with divalent ions, into microgel particles and microfibers using microfluidics. The microparticle morphology was highly affected by the polysaccharide concentration and its molecular weight. A model protein, namely Bovine...
Tipo: Text
Palavras-chave: Marine exopolysaccharide; Microgel particles; Microfibers; Microfluidic; Microencapsulation; Protein release.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00311/42175/41475.pdf

Exopolysaccharides produced by bacteria isolated from deep-sea hydrothermal vents: new agents with therapeutic potential
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean.
Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new...
Tipo: Text
Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf

Anti-Metastatic Properties of a Marine Bacterial Exopolysaccharide-Based Derivative Designed to Mimic Glycosaminoglycans
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Heymann, Dominique; Ruiz-velasco, Carmen; Chesneau, Julie; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia.
Osteosarcoma is the most frequent malignant primary bone tumor characterized by a high potency to form lung metastases. In this study, the effect of three oversulfated low molecular weight marine bacterial exopolysaccharides (OS-EPS) with different molecular weights (4, 8 and 15 kDa) were first evaluated in vitro on human and murine osteosarcoma cell lines. Different biological activities were studied: cell proliferation, cell adhesion and migration, matrix metalloproteinase expression. This in vitro study showed that only the OS-EPS 15 kDa derivative could inhibit the invasiveness of osteosarcoma cells with an inhibition rate close to 90%. Moreover, this derivative was potent to inhibit both migration and invasiveness of osteosarcoma cell lines; had no...
Tipo: Text
Palavras-chave: Exopolysaccharides; Glycosaminoglycan; Heparin-like; Derivatives; Sulfation; Bone metabolism; Bone remodeling; Lung mestatases; Osteosarcoma.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00333/44442/44112.pdf

Effect of an oversulfated exopolysaccharide on angiogenesis induced by fibroblast growth factor-2 or vascular endothelial growth factor in vitro
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Matou, Sabine; Colliec-jouault, Sylvia; Galy Fauroux, Isabelle; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, Anne-marie; Helley, Dominique.
The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube fort-nation was related to alpha(6)...
Tipo: Text
Palavras-chave: Matrigel; Collagen gel; Alpha 6 integrin subunit; Angiogenic growth factor; Polysaccharide; Angiogenesis.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2118.pdf

Characterization, chemical modifications and in vitro anticoagulant properties of an exopolysaccharide produced by Alteromonas infernus
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Colliec-jouault, Sylvia; Chevolot, Lionel; Helley, Dominique; Ratiskol, Jacqueline; Bros, Andrée; Sinquin, Corinne; Roger, Olivier; Fischer, Anne-marie.
A new low-molecular-weight 'heparin-like' component was obtained from an exopolysaccharide produced by a mesophilic strain found in deep-sea hydrothermal vents. Data concerning the structure of the native high-molecular-weight exopolysaccharide (10(6) g/mol, 10% sulfate content) are reported for the first time. Two depolymerization processes were used to obtain low-molecular-weight (24-35 x 10(3) g/mol) oversulfated fractions (sulfate content 20 or 40%), Nuclear magnetic resonance studies indicated that after sulfation (40%), the low-molecular-weight fraction obtained by free radical depolymerization was less sulfated in the 6-O-position than the fraction depolymerized by acid hydrolysis. The free radical depolymerized product also had sulfated residues in...
Tipo: Text
Palavras-chave: Sulfation; Sulfated polysaccharide; Serpin; Heparin like; Depolymerization; Anticoagulant activity; Affinity co electrophoresis.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-2111.pdf

Polysaccharide labelling: impact on structural and biological properties
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Roger, Olivier; Colliec-jouault, Sylvia; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, A; Chevolot, Lionel.
Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science...
Tipo: Text
Palavras-chave: Anticoagulant activity; Reductive amination; Fluorescent tagging; Fucan; GAG; Sulphated polysaccharide.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2113.pdf

Purification of the exopolysaccharide produced by Alteromonas infernus: identification of endotoxins and effective process to remove them
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Grivaud-le Du, Alicia; Zykwinska, Agata; Sinquin, Corinne; Ratiskol, Jacqueline; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome; Delbarre-ladrat, Christine; Colliec-jouault, Sylvia.
Alteromonas infernus bacterium isolated from deep-sea hydrothermal vents can produce by fermentation a high molecular weight exopolysaccharide (EPS) called GY785. This EPS described as a new source of glycosaminoglycan-like molecule presents a great potential for pharmaceutical and biotechnological applications. However, this unusual EPS is secreted by a Gram-negative bacterium and can be therefore contaminated by endotoxins, in particular the lipopolysaccharides (LPS). Biochemical and chemical analyses of the LPS extracted from A. infernus membranes have shown the lack of the typical LPS architecture since 3-deoxy-d-manno-oct-2-ulopyranosonic acid (Kdo), glucosamine (GlcN), and phosphorylated monosaccharides were not present. Unlike for other...
Tipo: Text
Palavras-chave: Marine Alteromonas infernus; Bacterial exopolysaccharide; Endotoxin; Characterization; Purification; Fermentation; Process.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00397/50818/51601.pdf

Unusual Glycosaminoglycans from a Deep Sea Hydrothermal Bacterium Improve Fibrillar Collagen Structuring and Fibroblast Activities in Engineered Connective Tissues
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Senni, Karim; Gueniche, Farida; Changotade, Sylvie; Septier, Dominique; Sinquin, Corinne; Ratiskol, Jacqueline; Lutomski, Didier; Godeau, Gaston; Guezennec, Jean; Colliec-jouault, Sylvia.
Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800...
Tipo: Text
Palavras-chave: Marine hydrothermal bacteria; Glycosaminoglycan-mimetic; Collagen; Matrix metalloproteinases; Dermal fibroblast; Polysaccharides; Tissue engineering.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00138/24973/23072.pdf

Structural data on a bacterial exopolysaccharide produced by a deep-sea Alteromonas macleodii strain
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Costaouec, Tinaig; Cerantola, Stephane; Ropartz, D.; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Boisset, Claire.
Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this...
Tipo: Text
Palavras-chave: Bacterial exopolysaccharide; Alteromonas macleodii; Structure determination; NMR spectroscopy; Mass spectrometry.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00095/20620/18536.pdf

The marine bacteria Cobetia marina DSMZ 4741 synthesizes an unexpected K-antigen-like exopolysaccharide
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lelchat, Florian; Cerantola, Stephane; Brandily, Christophe; Colliec-jouault, Sylvia; Baudoux, Anne-claire; Ojima, Takao; Boisset, Claire.
We have studied the exopolysaccharide produced by Cobetia marina DSMZ 4741, a marine bacterium isolated from coastal seawater. This strain is able to produce a polysaccharide in presence of carbon sources as glucose, mannitol and alginate. The maximum production occurs in aerobic condition, during the end of the exponential phase. The polymer is a non-viscous, acidic heteropolysaccharide of 270 kDa constituted of a repeating unit of: →2)-β-D-Ribf-(1→4)-[7,8-O-(Pyr)]-α-D-KDOp-(2→ This kind of chemical structure is generally related to K-antigen polysaccharide of pathogenic Escherichia coli strains. This is the first time this type of EPS is described from a marine bacterium. Moreover the polysaccharide exhibits a pyruvate substitution on its...
Tipo: Text
Palavras-chave: Bacterial exopolysaccharides; K-antigen; Cobetia marina; KDO; Marine polysaccharide.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00254/36494/35038.pdf