Consulta: creatorFacets:"Combik,Michal"
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Agrobacterium rhizogenes-mediated transformation of Hypericum sinaicum L. for the development of hairy roots containing hypericin
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Khlifa,Heba Desouky; Klimek-Chodacka,Magdalena; Baranski,Rafal; Combik,Michal; Taha,Hussein Sayed.
Hypericum sinaicum L. is an endangered Egyptian medicinal plant of high importance due to the presence of naphthodianthrones (hypericins), which have photodynamic properties and pharmaceutical potential. We sought to assess H. sinaicum ability to develop hairy roots that could be cultured in contained conditions in vitro and used as a source for hypericin production. We used four A. rhizogenes strains differing in their plasmids and chromosomal backgrounds to inoculate excised H. sinaicum root, stem and leaf explants to induce hairy root development. Additionally, inoculum was applied to shoots held in Rockwool cubes supporting their stand after removal of the root system. All explant types were susceptible to A. rhizogenes although stem explants responded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Medicinal plant; Naphthodianthrones; Plant genetic engineering.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100590