Consulta: creatorFacets:"Coser,Janaina"
Registros recuperados: 2
Data/hora: 10/06/2024 05:38:32

Human papillomavirus detection and typing using a nested-PCR-RFLP assay
Provedor de dados: 55
Autores: Coser,Janaina; Boeira,Thaís da Rocha; Fonseca,André Salvador Kazantzi; Ikuta,Nilo; Lunge,Vagner Ricardo.
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS: Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay. RESULTS: The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA probes; HPV polymerase chain reaction polymorphism; Restriction fragment length.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500009

Cervical human papillomavirus infection and persistence: a clinic-based study in the countryside from South Brazil
Provedor de dados: 55
Autores: Coser,Janaina; Boeira,Thaís da Rocha; Wolf,Jonas Michel; Cerbaro,Kamila; Simon,Daniel; Lunge,Vagner Ricardo.
Abstract Human papillomavirus (HPV) infection is common in sexually active women and viral persistence may cause intraepithelial lesions and eventually progress to cervical cancer (CC). The present study aimed to investigate epidemiological factors related to HPV infection and to evaluate viral persistence and CC precursor lesions frequencies in women from a city in the countryside of South Brazil. Three hundred women were recruited from a primary public health care clinic. The patients were interviewed and underwent sampling with cervical brushes for HPV-DNA detection/typing by a PCR-based assay and cytological analysis by Pap smear test. HPV was detected in 47 (15.7%) women. HPV infection was significantly associated with young age (&lt;30 years) and low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV prevalence; HPV persistence; Cervical cancer; Epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100061