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Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cunha,E.V.; Costa,J.J.N.; Rossi,R.O.D.S.; Silva,A.W.B.; Passos,J.R.S.; Portela,A.M.L.R.; Pereira,D.C.S.T.; Donato,M.A.M.; Campello,C.C.; Saraiva,M.V.A.; Peixoto,C.A.; Silva,J.R.V.; Santos,R.P.
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles culturedin vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter and survival, antrum formation, ultrastructure and expression of mRNA for FSH receptors (FSH-R), proliferating cell nuclear antigen (PCNA), and neuronal nitric oxide synthase were determined. All treatments maintained follicular survival [α-MEM+ (94.59%); 1 µg/mL PHA (96.43%); 10 µg/mL PHA (84.85%); 50 µg/mL PHA (85.29%); 100 µg/mL PHA (88.57%), and 200 µg/mL PHA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprine; Antrum formation; FSH-R; PCNA; Ultrastructure.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300245