Consulta: creatorFacets:"Coutinho,Luiz Lehmann"
Registros recuperados: 24
Data/hora: 09/06/2024 16:41:03

Molecular marker heterozygosities and genetic distances as correlates of production traits in F1 bovine crosses
Provedor de dados: Genetics and Molecular Biology
Autores: Tambasco-Talhari,Daniella; Alencar,Maurício Mello de; Paz,Cláudia Cristina Paro de; Cruz,Geraldo Maria da; Rodrigues,Armando de Andrade; Packer,Irineu Umberto; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida.
Several studies have investigated the relationship between heterozygosity, genetic distance and production traits. The objective of the present study was to evaluate the influence of the degree of heterozygosity and genetic distance on growth, carcass and reproductive related features in F1 bovine crosses. We tested 10 polymorphic markers in 330 purebred cattle (Nelore, Canchim, Aberdeen Angus and Simental) and 256 crossbred cattle belonging to four crossbred groups. Individual heterozygosities (Hi) and multilocus genetic similarity (Dm) were estimated and used in correlation analysis against individual phenotypic measurements. Significant (p &lt; 0.05) Hi effects occurred for birth weight, 15 to 18 month weight, hot carcass weight and longissimus rib eye...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine crosses; Molecular markers; Heterozygosity; Genetic distance; Production traits.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200007

Genetic characterization of Aberdeen Angus cattle using molecular markers
Provedor de dados: Genetics and Molecular Biology
Autores: Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida.
Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005

Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
Provedor de dados: Genetics and Molecular Biology
Autores: Zaros,Lilian Giotto; Bricarello,Patrízia Ana; Amarante,Alessandro Francisco Talamini; Coutinho,Luiz Lehmann.
T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytokine gene expression; Real-time RT-PCR; Cattle; Gastrointestinal nematodes; Bovine cytokines.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012

Cross-species microsatellite amplification in South American Caimans (Caiman spp and Paleosuchus palpebrosus)
Provedor de dados: Genetics and Molecular Biology
Autores: Zucoloto,Rodrigo Barban; Villela,Priscilla Marqui Schimidt; Verdade,Luciano Martins; Coutinho,Luiz Lehmann.
Microsatellite DNA markers have been used to assess genetic diversity and to study ecological behavioral characteristics in animals. Although these markers are powerful tools, their development is labor intensive and costly. Thus, before new markers are developed it is important to prospect the use of markers from related species. In the present study we investigated the possibility of using microsatellite markers developed for Alligator mississipiensis and Caiman latirostris in South American crocodilians. Our results demonstrate the use of microsatellite markers for Paleosuchus palpebrosus, Caiman crocodilus and Caiman yacare.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; STR; Primers; Crocodilians; Alligatorinae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100015

Quantitative trait locus affecting birth weight on bovine chromosome 5 in a F2 Gyr x Holstein population
Provedor de dados: Genetics and Molecular Biology
Autores: Gasparin,Gustavo; Miyata,Marcelo; Coutinho,Luiz Lehmann; Martinez,Mário Luiz; Silva,Marcos Vinícius G. Barbosa da; Machado,Marco Antônio; Campos,Ana Lúcia; Regitano,Luciana Correia de Almeida.
Segregation between a genetic marker and a locus influencing a quantitative trait in a well delineated population is the basis for success in mapping quantitative trait loci (QTL). To detect bovine chromosome 5 (BTA5) birth weight QTL we genotyped 294 F2 Gyr (Bos indicus) x Holstein (Bos taurus) crossbreed cattle for five microsatellite markers. A linkage map was constructed for the markers and an interval analysis for the presence of QTL was performed. The linkage map indicated differences in the order of two markers relative to the reference map (&lt;A HREF="http://www.marc.usda.gov/"&gt;http://www.marc.usda.gov&lt;/A&gt;). Interval analysis detected a QTL controlling birth weight (p &lt; 0.01) at 69 centimorgans (cM) from the most centromeric marker...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BTA5; Birth weight; Cattle; QTL; Microsatellite markers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500005

ESTs and putative line-specific (broiler and layer) SNPs identified in genes expressed in Gallus gallus pituitary and hypothalamus
Provedor de dados: Genetics and Molecular Biology
Autores: Cassoli,Clarissa Sanches da Silva; Jorge,Erika Cirstina; Patrício,Mateus; Alves,Helena Javiel; Almeida,Erik Amazonas de; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann.
Brazilian poultry industry has reached a high level of development in both meat and egg production as a result of constant technological modernization. Further improvements can be achieved through genomics, but before this can be accomplished, a better understanding of gene expression profiles and nucleotide polymorphisms is necessary. Since animal physiology is directly or indirectly controlled by the pituitary and hypothalamus, the aim of the present work was to identify and analyze genes expressed in these tissues in chicken lines with different growth potential. Two pituitary and hypothalamus cDNA libraries from 21 day broiler (TT) and layer (CC) chickens lines were constructed and allowed identification of 3,074 unique sequences and 77 single...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary; Hypothalamus; EST; SNP; Chicken.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600008

Quantitative trait loci (QTL) mapping for growth traits on bovine chromosome 14
Provedor de dados: Genetics and Molecular Biology
Autores: Miyata,Marcelo; Gasparin,Gustavo; Coutinho,Luiz Lehmann; Martinez,Mario Luiz; Machado,Marco Antonio; Silva,Marcos Vinicius G. Barbosa da; Campos,Ana Lucia; Sonstegard,Tad S.; Rosário,Millor Fernandes do; Regitano,Luciana Correia de Almeida.
Quantitative trait loci (QTL) mapping in livestock allows the identification of genes that determine the genetic variation affecting traits of economic interest. We analyzed the birth weight and weight at 60 days QTL segregating on bovine chromosome BTA14 in a F2 resource population using genotypes produced from seven microsatellite markers. Phenotypes were derived from 346 F2 progeny produced from crossing Bos indicus Gyr x Holstein Bos taurus F1 parents. Interval analysis to detect QTL for birth weight revealed the presence of a QTL (p &lt; 0.05) at 1 centimorgan (cM) from the centromere with an additive effect of 1.210 ± 0.438 kg. Interval analysis for weight at 60 days revealed the presence of a QTL (p &lt; 0.05) at 0 cM from the centromere with an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TA14; Cattle; F2; Growth traits; Molecular markers; QTL.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300011

k-Casein, b-lactoglobulin and growth hormone allele frequencies and genetic distances in Nelore, Gyr, Guzerá, Caracu, Charolais, Canchim and Santa Gertrudis cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Kemenes,Paola Augusta; Regitano,Luciana Correia de Almeida; Rosa,Artur Jordão de Magalhães; Packer,Irineu Umberto; Razook,Alexander George; Figueiredo,Leopoldo Andrade de; Silva,Nirlei Aparecida; Etchegaray,Maria Antônia L.; Coutinho,Luiz Lehmann.
The genotypes for k-casein (&lt;FONT FACE="Symbol"&gt;k&lt;/FONT&gt;-CN), &lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt;-lactoglobulin (&lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt;-LG) and growth hormone (GH) were determined by polymerase chain reaction (PCR) and restriction enzyme digestion in seven breeds of cattle (Nelore, Gyr, Guzerá, Caracu, Charolais, Canchim and Santa Gertrudis). &lt;FONT FACE="Symbol"&gt;k&lt;/FONT&gt;-Casein had two alleles with the A allele occurring at a higher frequency in Bos indicus breeds (0.93, 0.92 and 0.91% for Gyr, Guzerá and Nelore, respectively). The &lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt;-lactoglobulin locus had two alleles in all of the breeds. European breeds had a higher frequency of the &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400012

Identification of proliferating cells in chicken embryos using 5-bromo- 2'-deoxyuridine immunohistochemical detection
Provedor de dados: Genetics and Molecular Biology
Autores: Lopes,Mário Lúcio; Schmidt,Gilberto Silber; Coutinho,Luiz Lehmann.
Chicken embryos were incubated with BrdU, embedded in plastic resin, sectioned and screened immunohistochemically to identify proliferating cells in the neural tube and somites. Fixation in 4% paraformaldehyde for 1 h was essential for detecting specific colorimetric signals of BrdU incorporation into cells during the S phase of the cell cycle. Transverse sections of the neural tube showed that the nuclei of proliferating cells (BrdU positive) had a uniform and centralized distribution, whereas unstained nuclei were found only along the extremities of the neural tube. Transverse sections of differentiated somites showed proliferating cells in the scleratome and dermatome. However, no incorporation of BrdU was observed in myotomic cells, which give rise to...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100028

Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes
Provedor de dados: Genetics and Molecular Biology
Autores: Zaros,Lilian Giotto; Coutinho,Luiz Lehmann; Sider,Lúcia Helena; Medeiros,Henrique Rocha de; Neves,Maria Rosalba Moreira das; Benvenuti,Camila Loures; Navarro,Andrine Maria do Carmo; Vieira,Luiz da Silva.
Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Control genes; Gastrointestinal nematodes; Ovine; Real-time RT-PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300018

Regiões genômicas associadas a características de desempenho e carcaça no cromossomo 5 de linhagens Brasileiras de galinha
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Silva,Fernanda Eliza de Jesus; Guido,Luiza Nicolosi; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann; Rosário,Millor Fernandes do.
O objetivo deste trabalho foi caracterizar genotipicamente, e construir o mapa de ligação e mapear locos associados a características quantitativas (QTL) de desempenho e carcaça no cromossomo 5 de linhagens brasileiras galinhas. Utilizou-se uma população F2 CTCT, resultante do acasalamento entre machos da linhagem de postura CC e fêmeas da linhagem de corte TT. Um total de 356 animais foi genotipado com 11 marcadores microssatélites. A caracterização genotípica foi realizada pela estimação dos seguintes parâmetros genotípicos: conteúdo de informação polimórfica (0,45-0,69), heterozigosidades observada (0,48-1,00) e esperada (0,48-0,74), e número de alelos por loco (3-5). Empregou-se o mapeamento por intervalo combinado à modelagem fenotípica por modelo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avicultura; Mapeamento por intervalo; Marcadores microssatélites; Locos de características quantitativas.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000300002

Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Sousa Junior,Luis Paulo Batista; Meira,Ariana Nascimento; Azevedo,Hymerson Costa; Muniz,Evandro Nevez; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista.
Abstract: The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovis aries; Candidate genes; Frequencies; Variants.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102810

Efeito do genótipo halotano, da ractopamina e do sexo do animal na qualidade da carne suína
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Bridi,Ana Maria; Oliveira,Audiléia Rocha de; Fonseca,Nilva Aparecida Nicolao; Shimokomaki,Massami; Coutinho,Luiz Lehmann; Silva,Caio Abércio da.
Este trabalho foi realizado com o objetivo de avaliar o efeito da adição de ractopamina na ração sobre a qualidade da carne de suínos dos genótipos halotano homozigoto dominante (HalNN) e heterozigoto (HalNn). Durante o experimento (21 dias), 24 suínos machos castrados e 12 fêmeas (metade de cada genótipo) com peso médio inicial de 72,6 kg de PV foram avaliados segundo delineamento experimental em blocos casualizados, em arranjo fatorial 2 x 2 x 2 (dois genótipos halotano; duas rações: controle e com adição de 10 ppm de ractopamina; e dois sexos: machos castrados e fêmeas). A análise do DNA genômico foi realizada por intermédio das técnicas de PCR-RLPC. A avaliação da qualidade da carne foi realizada no músculo Longissimus dorsi e o pH da carne foi medido...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carne PSE; Gene halotano; Qualidade da carne.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982006000700021

Mapping quantitative trait loci in Gallus gallus using principal components
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Pinto,Luís Fernando Batista; Packer,Irineu Umberto; Ledur,Mônica Corrêa; Moura,Ana Silvia Alves Meira Tavares; Nones,Kátia; Coutinho,Luiz Lehmann.
This study aimed at mapping QTL (quantitative trait loci) using linear combinations of characteristics of economical interest in Gallus gallus. A total of 350 F2 chickens from an initial crossing among males from a broiler line (TT) with females from a layer line (CC) were used. It was conducted a QTL mapping in chromosomes of Gallus gallus (GGA1, GGA3, GGA5, GGA8, GGA11, and GGA13) for 20 performance and carcass traits. For detecting QTL, it was used the likelihood ratio test between a reduced model (including fixed effects of sex, hatch and random effect of infinitesimal genetic value) and a full model (including all the previous effects plus QTL effects). When original characterists were analyzed, that is, before the formation of linear combinations,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broilers; Canonical variables; Chickens; Genome.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001100016

Caracterização molecular de animais da raça Nelore utilizando microssatélites e genes candidatos
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Tambasco,Daniella Debenedetti; Alencar,Maurício Mello de; Coutinho,Luiz Lehmann; Tambasco,Antonio Junqueira; Tambasco,Marina Debenedetti; Regitano,Luciana Correia de Almeida.
No presente trabalho, 180 fêmeas da raça Nelore, provenientes de oito rebanhos, foram analisadas quanto aos marcadores microssatélites TEXAN15, BM1224 e CSFM50 e aos polimorfismos de comprimento de fragmentos de restrição (RFLP) nos locos k-caseína, beta-lactoglobulina e hormônio de crescimento (GH). Com exceção de GH, todos os marcadores foram polimórficos na amostra estudada. Os valores de heterozigosidade, diversidade gênica, conteúdo de informação polimórfica (PIC) e probabilidade de exclusão de paternidade (PE) foram estimados. Os maiores valores de PIC (0,685) e PE (0,521) foram obtidos para o marcador BM1224.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; DNA; Marcador; Microssatélite; Nelore; RFLP.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982000000400014

Investigation of Leptin gene in broiler and layer chicken lines
Provedor de dados: Scientia Agricola
Autores: Ninov,Kerli; Ledur,Mônica Corrêa; Alves,Helena Javiel; Rosário,Millor Fernandes do; Nones,Kátia; Coutinho,Luiz Lehmann.
Leptin, a polypeptide hormone secreted mainly by adipose tissue, plays an important role in feed intake regulation, energy metabolism and reproduction in several species. Its function has been intensively studied in mammals; however, in birds limited information is available. The cDNA sequence for chicken leptin has been reported, and high hepatic expression levels of leptin were associated with fat deposition in selected bird lines. However, controversies still remain concerning to the chicken leptin gene and several authors failed to amplify this gene from genomic DNA or cDNA. In view of this controversy and the importance of this gene, the present study aimed to investigate the leptin gene in a population of birds developed by Embrapa Swine and Poultry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallus gallus; Fat deposition; Primer design.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200016

Genetic linkage maps of chicken chromosomes 6, 7, 8, 11 and 13 from a Brazilian resource population
Provedor de dados: Scientia Agricola
Autores: Ambo,Marcel; Campos,Raquel de Lello Rocha; Moura,Ana Sílvia Meira Tavares; Boschiero,Clarissa; Rosário,Millor Fernandes do; Ledur,Mônica Corrêa; Nones,Kátia; Coutinho,Luiz Lehmann.
A linkage map is essential not only for quantitative trait loci (QTL) mapping, but also for the organization and location of genes along the chromosomes. The present study is part of a project whose major objective is, besides from construction the linkage maps, the whole genome scan for mapping QTL for performance traits in the Brazilian experimental chicken population. Linkage maps of chicken chromosomes 6 to 8, 11 and 13 were constructed based on this population. The population was developed from two generations of crossbreeding between a broiler and a layer line. Fifty-one microsatellite markers were tested, from which 28 were informative: 4, 8, 7, 4 and 5 for chromosomes 6, 7, 8, 11 and 13, respectively. A SNP located in the leptin receptor gene was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallus gallus; Quantitative trait loci (QTL); Genetic maps; Microsatellite markers; Animal breeding.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500001

Genetic linkage map of chicken chromosome 1 from a Brazilian resource population
Provedor de dados: Scientia Agricola
Autores: Nones,Kátia; Ledur,Mônica Corrêa; Ruy,Deborah Clea; Baron,Erica Elias; Moura,Ana Silvia Alves Meira Tavares; Coutinho,Luiz Lehmann.
A genetic map provides insight into genome organization and chromosomal location of markers and genes, and is important for quantitative trait loci (QTL) mapping. The objective of this study was to use a Brazilian resource population to construct a linkage map for chicken chromosome 1. Eighty microsatellite markers were tested and 26 informative markers were typed in an experimental F2 population developed by two generations of crossbreeding between a broiler sire line and a layer line. A total of 649 F2 individuals from seven full-sib families with about 95 F2 offspring each were genotyped. Multi-locus linkage analysis resulted in a chromosome 1 map of 26 ordered markers. Locus order was in general agreement with other published linkage maps, except for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Poultry; Mapping.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100003

Variants in GH, IGF1, and LEP genes associated with body traits in Santa Inês sheep
Provedor de dados: Scientia Agricola
Autores: Machado,Alessandro Lima; Meira,Ariana Nascimento; Jucá,Adriana de Farias; Azevedo,Hymerson Costa; Muniz,Evandro Neves; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista.
ABSTRACT: Growth hormone (GH), insulin-like growth factor 1 (IGF1), and leptin (LEP) can be candidate genes for association studies because they play vital roles in the metabolism process. Thus, this study aimed to identify variants in these genes associated with body traits in Santa Inês sheep. The following were recorded: body weight at 100 (BW100) and 240 days (BW240), average daily gain (ADG), withers (WH) and croup (CH) heights, body length (BL), thoracic (TG) and leg (LG) girths, thoracic (TW) and croup (CW) widths, body depth (BD), rib eye area (REA), fat thickness (FT), and carcass finishing score (CFS). Single-locus association analysis was performed with 11 variants in IGF1, 18 in LEP, and 16 in GH. Moreover, two haplotypes in IGF1 and one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hormone; Carcass; Growth; Haplotype; Ovine.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000300401

Strategic marker selection to detect quantitative trait loci in chicken
Provedor de dados: Scientia Agricola
Autores: Ruy,Deborah Clea; Nones,Kátia; Baron,Erica Elias; Ledur,Mônica Corrêa; Melo,Cláudio Manoel Rodrigues de; Ambo,Marcel; Campos,Raquel de Lello Rocha; Coutinho,Luiz Lehmann.
Selective genotyping for a certain trait in individuals with extreme phenotypes contributes sufficient information to determine linkage between molecular markers and quantitative trait loci (QTL). In this experiment an F2 population, developed by crossing males from a broiler line with females from a layer line, was employed to detect QTL on chromosomes 3 and 5. Twenty-eight performance and carcass traits were measured in F2 offspring, and phenotypic correlations between traits were calculated. Body weight at 42 days (BW42) presented the greatest positive correlations with most other traits, with correlation between body weights at 35 and 41 days, weight gain between birth and 35, 41 and 42 days, as well as weights of carcass and some body parts superior...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QTL; Broiler; Selective genotyping; Body weight.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200003

Genotypic characterization of microsatellite markers in broiler and layer selected chicken lines and their reciprocal F1s
Provedor de dados: Scientia Agricola
Autores: Rosário,Millor Fernandes do; Ledur,Mônica Corrêa; Moura,Ana Silvia Alves Meira Tavares; Coutinho,Luiz Lehmann; Garcia,Antonio Augusto Franco.
Chicken experimental populations have been developed worldwide for QTL mapping, but their genotypic characterizations are not usually discussed. The objective of this study was to characterize genotypically two F1 reciprocal generations and their parental lines based on the estimation of genotypic parameters. These F1 generations originated two Brazilian reference populations to map QTL. The evaluated parameters were polymorphic information content (PIC), observed and expected heterozygosities and number of alleles at microsatellite loci on chromosomes 1, 3 and 4. All parental and F1 chickens from both populations were used totalling of 83 chickens: 14 from a broiler (TT) and 14 from a layer line (CC) and 55 from their reciprocal F1 generations. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PIC; Genotypic diversity; Heterozygosity; Number of alleles.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200002

Molecular cloning of αRYR hotspot region 1 from broiler chicken
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Ziober,Iris Lamberti; Paião,Fernanda Gonzales; Marin,Silvana Regina Rockenbach; Marchi,Denis Fabricio; Binneck,Eliseu; Nepomuceno,Alexandre Lima; Coutinho,Luiz Lehmann; Shimokomaki,Massami.
Samples of Pectoralis major m. were collected, and an RT-PCR analysis of the a-Ryanodine receptor (a RYR) from chicken mRNA hotspot region spanning aminoacid residues 386 to 540, numbered according to the turkey sequence, revealed two classes of transcripts. The sequences of the first class were similar to turkey and human with 97% and 74% of identity, respectively, and included all transcripts with substitutions in the nucleotide sequence. The second class was characterized by the deletion of nucleotides, leading to a premature stop codon and coding for a truncated and nonfunctional protein. These results are to date the first report related to the sequencing of the chicken αRYR hotspot region 1, which will possibility serve as a guide for further studies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ΑRYR; PSE; Chicken.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700029

Temporal expression pattern of the insulin-like growth factor II and fibroblast growth factor transcripts in avian embryogenesis
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Gabriel,Jane Eyre; Alvares,Lúcia Elvira; Coutinho,Luiz Lehmann.
In this study, the abundance of IGF-II and bFGF transcripts was estimated in the chicken embryos using the competitive RT-PCR analysis. Significant enhancements in the abundance of IGF-II mRNA were observed at stages HH1 and 5, and a new accumulation in these levels was observed at stage HH18 in comparison to the basal levels. The abundance of bFGF mRNA increased significantly at stages HH18 and 20, followed by an upregulation in the expression of these transcripts at stage HH26. These findings provided important information about the temporal expression pattern of IGF-II and bFGF transcripts in the whole chicken embryos during in ovo development.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken embryos; Temporal expression; Competitive RT-PCR; Growth factors.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500011

Brazilian coffee genome project: an EST-based genomic resource
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães.
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; CDNA; EST; Transcriptome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008