Consulta: creatorFacets:"Cruz,Rubens"
Registros recuperados: 3
Data/hora: 06/06/2024 00:39:19

Production and action pattern of inulinase from Aspergillus Niger-245: hydrolysis of inulin from several sources
Provedor de dados: Revista de Microbiologia
Autores: Cruz,Vinícius D’Arcadia; Belote,Juliana Gisele; Belline,Márcia Zilioli; Cruz,Rubens.
A strain of Aspergillus niger isolated from soil samples showed great capacity to produce extracellular inulinase. Although the enzyme has been synthesized in presence of monosaccharides, sucrose and sugar cane molasse, the productivity was significantly higher (p&amp;lt;0.05) when the microorganism was inoculated in media formulated with dahlia extract and pure inulin, as carbon sources. With regard to the nitrogen source, the best results were obtained with casein and other sources of proteic nitrogen, comparatively to the mineral nitrogen. However, statistic significance (p&amp;lt;0.01) only was found between the productivity obtained in the medium prepared with casein and ammonium sulphate. The optimum pH of the purified enzyme for inulin hydrolysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inulinase; Aspergillus niger; Fructose syrup; Inulin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400013

Properties of a new fungal b-galactosidase with potential application in the dairy industry
Provedor de dados: Revista de Microbiologia
Autores: Cruz,Rubens; Cruz,Vinícius D'Arcádia; Belote,Juliana Gisele; Khenayfes,Marcelo de Oliveira; Dorta,Claudia; Oliveira,Luiza Helena dos Santos.
&lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;-Galactosidase or &lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;-D-galactoside-galactohydrolase (EC. 3.2.1.23) is an important enzyme industrially used for the hydrolysis of lactose from milk and milk whey for several applications. Lately, the importance of this enzyme was enhanced by its galactosyltransferase activity, which is responsible for the synthesis of transgalactosylated oligosaccharides (TOS) that act as functional foods, with several beneficial effects on consumers. Penicillium simplicissimum, a strain isolated from soil, when grown in semi-solid medium showed good productivity of &lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;-galactosidase with galactosyltransferase activity. The optimum pH for hydrolysis was in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: &lt; FONT FACE=Symbol&gt; B&lt; /font&gt; -Galactosidase galactosyltransferase galactooligosaccharides lactose prebiotic effect.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300014

Purification and characterization of β-Fructosidase with inulinase activity from Aspergillus niger - 245
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Cruz,Vinícius D'Arcadia; Belote,Juliana Gisele; Dorta,Claudia; Santos,Luíza Helena Oliveira dos; Andriolo,Cláudia Regina; Khenayfes,Marcelo de Oliveira; Cruz,Rubens.
Aspergillus niger - 245, a strain isolated from soil samples showed good β-fructosidase activity when inoculated in medium formulated with dahlia extract tubers. The enzyme was purified by precipitation in ammonium sulphate and percolated in DEAE-Sephadex A-50 and CM-cellulose columns, witch showed a single peack in all the purification steps, maintaining the I/S ratio between 0.32 to, 0.39. Optimum pH for inulinase activity (I) was between 4.0 - 4.5 and for invertase activity (S) between 2.5 and 5.0. The optimum temperature was 60O.C for both activities and no loss in activity was observed when it was maintained at this temperature for 30 min. The Km value was 1.44 and 5.0, respectively, for I and S and Vm value 10.48 and 30.55, respectively. The I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inulinase; Invertase; Β-fructosidase; Inulin; Fructose syrup; Aspergillus niger.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300003