Consulta: creatorFacets:"Cunha,Maria de Lourdes Ribeiro de Souza da"
Registros recuperados: 7
Data/hora: 02/06/2024 11:57:57

Importância do Staphylococcus aureus nas mastites subclínicas: pesquisa de enterotoxinas e toxina do choque tóxico, e a relação com a contagem de células somáticas
Provedor de dados: 62
Autores: Sá,Marcos Eielson Pinheiro de; Cunha,Maria de Lourdes Ribeiro de Souza da; Elias,Acacia Orieth; Victória,Cassiano; Langoni,Helio.
O Staphylococcus aureus é um dos principais agentes das mastites consideradas contagiosas, apresentando elevada incidência na maioria dos rebanhos leiteiros em vários países. Além de perdas econômicas é importante salientar o aspecto de saúde pública para cepas produtoras de enterotoxinas e da toxina do choque tóxico. A enterotoxina A, relacionada com maior ênfase nos casos de toxinfecções alimentares, pode ser veiculada pelo leite cru, pasteurizado e subprodutos lácteos. A síndrome do choque tóxico é determinada mais freqüentemente pela toxina do choque tóxico, porém as enterotoxinas do tipo B e C também podem ser implicadas. O objetivo deste estudo foi verificar a ocorrência de S.aureus produtores de enteroxinas e da toxina do choque tóxico em amostras...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastite; Subclinica; Bovino; Staphylococcus aureus; Enterotoxinas.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000500005

Detection of the mecA gene and identification of Staphylococcus directly from blood culture bottles by multiplex polymerase chain reaction
Provedor de dados: 55
Autores: Rocchetti,Taisa Trevizani; Martins,Katheryne Benini; Martins,Patricia Yoshida Faccioli; Oliveira,Rogério Antonio de; Mondelli,Alessandro Lia; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da.
ABSTRACT  Introduction: Staphylococcus spp. – both S. aureus, including methicillin-resistant strains (MRSA) and coagulase negative staphylococci (CoNS) – are relevant agents of healthcare-associated infections. Therefore, the rapid recognition of MRSA and methicillin-resistant CoNS from blood stream infections is critically important for patient management. It is worth noting that inappropriate empiric therapy has been associated with higher in-hospital mortality.  Material and methods: In this study we evaluated a multiplex polymerase chain reaction (multiplex PCR) standardized to detect Staphylococcus spp., S. aureus, and mecA gene-encoded oxacillin resistance directly from blood culture bottles. A total of 371 blood cultures with Gram-positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus spp.; Staphylococcus aureus; MRSA; MecA gene; Blood cultures; Multiplex PCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200099

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from a Brazilian university hospital
Provedor de dados: 55
Autores: Martins,André; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Cunha,Maria de Lourdes Ribeiro de Souza da.
The aim of this study was to perform SCCmec typing in Staphylococcus aureus isolates and to characterize the clonal profile of these isolates. Forty-six mecA gene-positive strains isolated between 2002 and 2006 were submitted to antimicrobial resistance testing by the E-test, SCCmec typing by multiplex PCR, and clonal profile analysis by pulsed-field gel electrophoresis. Forty-one (89.1%) isolates were typed as SCCmec III and five (10.9%) as SCCmec IV. Four circulating clones were detected, one of them comprising isolates related to the Brazilian epidemic clone. This clone was detected throughout the study period. The SCCmec III isolates were associated with a high rate of multidrug resistance and clonal dissemination of methicillin-resistant S. aureus in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylococcus aureus; MRSA; SCCmec; BEC.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300331

Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections
Provedor de dados: 58
Autores: Bonesso,Mariana Fávero; Marques,Silvio Alencar; Camargo,Carlos Henrique; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medical School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Resistance; Skin infections; Virulence; Epidemiology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400034

Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
Provedor de dados: 58
Autores: Martins,Katheryne Benini; Faccioli-Martins,Patricia Yoshida; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Fernandes,Simone; Oliveira,Aline A.; Dantas,Ariane; Zafalon,Luiz Francisco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Sheep; Staphylococcus aureus; Antibiotic resistance; Virulence factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535

Correlation of phenotypic tests with the presence of the blaZ gene for detection of beta-lactamase
Provedor de dados: 58
Autores: Ferreira,Adriano Martison; Martins,Katheryne Benini; Silva,Vanessa Rocha da; Mondelli,Alessandro Lia; Cunha,Maria de Lourdes Ribeiro de Souza da.
Abstract Staphylococcus aureus and Staphylococcus saprophyticus are the most common and most important staphylococcal species associated with urinary tract infections. The objective of the present study was to compare and to evaluate the accuracy of four phenotypic methods for the detection of beta-lactamase production in Staphylococcus spp. Seventy-three strains produced a halo with a diameter ≤28 mm (penicillin resistant) and all of them were positive for the blaZ gene. Among the 28 susceptible strain (halo ≥29 mm), 23 carried the blaZ gene and five did not. The zone edge test was the most sensitive (90.3%), followed by MIC determination (85.5%), but the specificity of the former was low (40.0%). The nitrocefin test was the least sensitive (28.9%)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary tract infection; Staphylococcus saprophyticus; Zone edge test; Beta-lactamase; BlaZ.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100159

Detection of enterotoxins genes in coagulase-negative staphylococci isolated from foods
Provedor de dados: 58
Autores: Cunha,Maria de Lourdes Ribeiro de Souza da; Peresi,Eliana; Calsolari,Regina Adriana Oliveira; Araújo Júnior,João Pessoa.
Staphylococcal food poisoning is caused by ingestion of enterotoxins preformed in the food contaminated essentially through human manipulation or raw material obtained from animals. Although coagulase-positive Staphylococcus aureus is the main agent responsible for food intoxication, some researches emphasise that coagulase-negative staphylococci (CNS) are able to produce staphylococcal enterotoxins and may be a potential cause of food poisoning. In the present study CNS were isolated from foods and the toxigenic capacity of the strains determined. A total of 88 food samples were analysed and 22.7% were positive for CNS strains. Staphylococcal counts ranged from 3.0 x 10² to 1.4 x 10(6) CFU/g or mL of food examined. S. epidermidis predominated among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulase-negative staphylococci; PCR; Enterotoxins.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100013