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Capillary electrophoresis determination of the binding affinity of bioactive sulfated polysaccharides to proteins: study of the binding properties of fucoidan to antithrombin
Provedor de dados: 5
Autores: Varenne, A; Gareil, P; Colliec-jouault, Sylvia; Daniel, R.
The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. This method is a variant of zonal electrophoresis in the mobility shift format. A fixed amount of protein was injected into a capillary filled with a background electrolyte containing the polysaccharide in varying concentrations. The effective mobility data of the protein were processed according to classical linearization treatments to...
Tipo: Text
Palavras-chave: Antithrombin; Fucoidan; Electrophoresis; Protein interaction; Polysaccharides.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-2114.pdf