Consulta: creatorFacets:"David, P"
Registros recuperados: 2
Data/hora: 11/06/2024 01:29:01

Mark-recapture cloning: a straightforward and cost-effective cloning method for population genetics of single-copy nuclear DNA sequences in diploids
Provedor de dados: 5
Autores: Bierne, Nicolas; Tanguy, A; Faure, Michel; Faure, B; David, E; Boutet, I; Boon, E; Quere, N; Plouviez, S; Kemppainen, P; Jollivet, D; Moraga, D; Boudry, Pierre; David, P.
We describe a simple protocol to reduce the number of cloning reactions of nuclear DNA sequences in population genetic studies of diploid organisms. Cloning is a necessary step to obtain correct haplotypes in such organisms, and, while traditional methods are efficient at cloning together many genes of a single individual, population geneticists rather need to clone the same locus in many individuals. Our method consists of marking individual sequences during the polymerase chain reaction (PCR) using 5'-tailed primers with small polynucleotide tags. PCR products are mixed together before the cloning reaction and clones are sequenced with universal plasmid primers. The individual from which a sequence comes from is identified by the tag sequences upstream...
Tipo: Text
Palavras-chave: Sequence; Population genetics; High throughput allele recognition; DNA polymorphism.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-4222.pdf

Acoustic backscattering from salp and target strength estimation
Provedor de dados: 5
Autores: David, P; Guerin-ancey, O; Oudot, G; Van Cuyck, Jp.
Although zooplanktonic organisms nearly have the acoustical impedance of water, they backscatter the sound. Strong acoustical echoes from salps were recorded in the Weddell sea at 150 kHz (1992), in the Indian ocean at 12 kHz (1994) and in the Mediterranean at 120 and 38 kHz (1996). Sphere and cylinder models were applied to estimate the salp target strength. The salp is modelled by a cylindrical gelatinous body (length L) and a spherical nucleus (radius a). Backscattering from each part was estimated individually. With a detection threshold of -100 dB, the minimum body length and the minimum radius of nucleus to be theoretically detected were estimated for each of the following frequencies: 12, 38, 120 and 150 kHz. A small salp (L = 14 rum and a = I mm)...
Tipo: Text
Palavras-chave: Salpe; Target strength; Acoustique sous-marine; Salp; Target strength; Underwater acoustics.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00322/43341/42886.pdf