Consulta: creatorFacets:"Divakar, P.K."
Registros recuperados: 2
Data/hora: 10/06/2024 01:32:10

New primers for promising single-copy genes in fungal phylogenetics and systematics
Provedor de dados: 16
Autores: Schmitt, I.; Crespo, A.; Divakar, P.K.; Fankhauser, J.D.; Herman-Sackett, E.; Kalb, K.; Nelsen, M.P.; Nelson, N.A.; Rivas-Plata, E.; Shimp, A.D.; Widhelm, T.; Lumbsch, H.T.
Developing powerful phylogenetic markers is a key concern in fungal phylogenetics. Here we report degenerate primers that amplify the single-copy genes Mcm7 (MS456) and Tsr1 (MS277) across a wide range of Pezizomycotina (Ascomycota). Phylogenetic analyses of 59 taxa belonging to the Eurotiomycetes, Lecanoromycetes, Leotiomycetes, Lichinomycetes and Sordariomycetes, indicate the utility of these loci for fungal phylogenetics at taxonomic levels ranging from genus to class. We also tested the new primers in silico using sequences of Saccharomycotina, Taphrinomycotina and Basidiomycota to predict their potential of amplifying widely across the Fungi. The analyses suggest that the new primers will need no, or only minor sequence modifications to amplify...
Tipo: Article / Letter to the editor
Palavras-chave: Ascomycota; DNA replication licensing factor; Evolution; Lichenised fungi; Mcm7; MS277; MS456; Phylogeny; Pre-rRNA processing protein; Protein-coding; Tsr1.
Ano: 2009
URL: http://www.repository.naturalis.nl/record/531799

Evaluating methodologies for species delimitation: the mismatch between phenotypes and genotypes in lichenized fungi (Bryoria sect. Implexae, Parmeliaceae)
Provedor de dados: 16
Autores: Boluda, C.G.; Rico, V.J.; Divakar, P.K.; Nadyeina, O.; Myllys, L.; McMullin, R.T.; Zamora, J.C.; Scheidegger, C.; Hawksworth, D.L.
In many lichen-forming fungi, molecular phylogenetic analyses lead to the discovery of cryptic species within traditional morphospecies. However, in some cases, molecular sequence data also questions the separation of phenotypically characterised species. Here we apply an integrative taxonomy approach ‒ including morphological, chemical, molecular, and distributional characters ‒ to re-assess species boundaries in a traditionally speciose group of hair lichens, Bryoria sect. Implexae. We sampled multilocus sequence and microsatellite data from 142 specimens from a broad intercontinental distribution. Molecular data included DNA sequences of the standard fungal markers ITS, IGS, GAPDH, two newly tested loci (FRBi15 and FRBi16), and SSR frequencies from 18...
Tipo: Article / Letter to the editor
Palavras-chave: Chemotypes; Cryptic species; Haplotypes; Incomplete lineage sorting; Integrative taxonomy; Microsatellites; Speciation; Species concepts.
Ano: 2019
URL: http://www.repository.naturalis.nl/record/660277