Consulta: creatorFacets:"Donald C. Cooper"
Registros recuperados: 11
Data/hora: 13/06/2024 03:45:54

A novel apparatus/protocol designed for optogenetic manipulation and recording of individual neurons during a motivation and working memory task in the rodent
Provedor de dados: Nature Precedings
Autores: Samuel Dolzani; Donald C. Cooper.
Innovative molecular tools allow neuroscientists to study neural circuitry associated with specific behaviors. Consequently, behavioral methods must be developed to interface with these new molecular tools in order for neuroscientists to identify the causal elements underlying behavior and decision-making processes. Here we present an apparatus and protocol for a novel Go/No-Go behavioral paradigm to study the brain attention and motivation/reward circuitry in awake, head-restrained rodents. This experimental setup allows: (1) Painless and stable restraint of the head and body; (2) Rapid acquisition to simple or complex operant tasks; (3) Repeated electrophysiological single and multiple unit recordings during ongoing behavior; (4) Pharmacological and...
Tipo: Manuscript
Palavras-chave: Neuroscience; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/7152/version/1

Changing the Culture of &amp;#x201c;Ding&amp;#x201d;: Education, Legislation and Research on Concussive Brain Injury in Youth Athletics
Provedor de dados: Nature Precedings
Autores: Claire D. Overturf; Donald C. Cooper.
The number of emergency department visits concerning sport related TBI in youth increased 57% from 2001 to 2009 and while a fraction of that increase may be attributed to injuries that were once missed now being identified due to greater general awareness, the fact remains that there are nearly 200,000 children every year who suffer sport related concussion or other TBI serious enough to prompt a visit to the ED. 40% of sports related concussions involve children between the ages of 8 and 13, and in this group the rate of concussion doubled between 1997 and 2007. The risk of concussion is highest in football and there are nearly 67,000 diagnosed concussions in high school football every year. In other sports that males and females play, such as basketball,...
Tipo: Manuscript
Palavras-chave: Neuroscience.
Ano: 2012
URL: http://precedings.nature.com/documents/7076/version/1

Cocaine self-administration in the mouse: A low-cost, chronic catheter preparation
Provedor de dados: Nature Precedings
Autores: Matthew B. Pomrenze; Michael  V. Baratta; Brian A. Cadle; Donald C. Cooper.
Intravenous drug self-administration is the most valid animal model of human addiction because it allows volitional titration of the drug in the blood based on an individual&amp;#x2019;s motivational state together with the pharmacokinetic properties of the drug. Here we describe a reliable low-cost mouse self-administration catheter assembly and protocol that that can be used to assess a variety of drugs of abuse with a variety of protocols. We describe a method for intravenous catheter fabrication that allows for efficient and long-lasting intravenous drug delivery. The intravenous catheters remained intact and patent for several weeks allowing us to establish stable maintenance of cocaine acquisition. This was followed by a dose response study in the...
Tipo: Manuscript
Palavras-chave: Biotechnology; Genetics &amp; Genomics; Molecular Cell Biology; Neuroscience; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/7040/version/1

mGluR5 knockout mice exhibit normal conditioned place-preference to cocaine
Provedor de dados: Nature Precedings
Autores: Melissa  A. Fowler; Andrew L. Varnell; Donald C. Cooper.
Metabotropic glutamate receptor 5 (mGluR5) null mutant (-/-) mice have been reported to totally lack the reinforcing or locomotor stimulating effects of cocaine. We tested mGluR5 -/- and +/+ mice for their locomotor and conditioned place- preference response to cocaine. Unlike the previous finding, here we show that compared to mGluR5 +/+ mice, -/- mice exhibit no difference in the locomotor response to low to moderate doses of cocaine (10 or 20 mg/kg). A high dose of cocaine (40 mg/kg) resulted in a blunted rather than absent locomotor response. We tested mGluR5 -/- and +/+ mice for conditioned place-preference to cocaine and found no group differences at a conditioning dose of 10 mg/kg, suggesting normal conditioned rewarding properties of cocaine. These...
Tipo: Manuscript
Palavras-chave: Neuroscience; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/6180/version/1

Sociability is decreased following deletion of the _trpc4_ gene
Provedor de dados: Nature Precedings
Autores: Kristin C. Rasmus; Jun-Gang  Wang; Andrew L. Varnell; Eric M. Ostertag; Donald C. Cooper.
Shyness and social anxiety are predominant features of some psychiatric disorders including autism, schizophrenia, anxiety and depression. Understanding the cellular and molecular determinants of sociability may reveal therapeutic approaches to treat individuals with these disorders and improve their quality of life. Previous experiments from our laboratory have identified selective mRNA and protein expression of a nonselective cation channel known as the canonical transient receptor potential channel 4 (TRPC4s) in brain regions implicated in emotional regulation and anxiety. TRPC4 is highly expressed in the corticolimbic regions of the mammalian brain. We hypothesized that robust corticolimbic expression of TRPC4 may regulate the brain&amp;#x2019;s...
Tipo: Manuscript
Palavras-chave: Genetics &amp; Genomics; Neuroscience.
Ano: 2011
URL: http://precedings.nature.com/documents/6367/version/1

Deletion of the rat trpc4 gene and its influence on motivated responding for natural reward
Provedor de dados: Nature Precedings
Autores: William D. Klipec; Bridget Deeney; Claire  Williamson; Kami  Wenzel; Phuong Nguyen; Eric Ostertag; Donald C. Cooper.
TRPC4 ion channels are expressed extensively in corticolimbic brain regions and a subpopulation of midbrain dopamine neurons. TRPC4 knockout (KO) rats show reduced sociability and social exploration, but show no differences in simple and complex strategic learning compared to normal wild type (WT) rats. Using water reward, we found no differences between TRPC4-KO and WT rats in the break point on a progressive ratio schedule of reinforcement. Although deletion of the trpc4 gene alters social interaction/anxiety it does not appear to affect motivation for natural rewards. Current experiments are underway testing the role of trpc4 gene deletion on cocaine reward (see www.neuro-cloud.net/nature-precedings/klipec3 for updates and collaborations).
Tipo: Manuscript
Palavras-chave: Genetics &amp; Genomics; Neuroscience.
Ano: 2012
URL: http://precedings.nature.com/documents/7109/version/1

Gulf War Syndrome: A role for organophosphate induced plasticity of locus coeruleus neurons
Provedor de dados: Nature Precedings
Autores: Jun-li  Cao; Andrew L. Varnell; Donald C. Cooper.
Gulf War syndrome is a chronic multi-symptom illness that has affected about a quarter of the deployed veterans of the 1991 Gulf War. Exposure to prolonged low-level organophosphate insecticides and other toxic chemicals is now thought to be responsible. Chlorpyrifos was one commonly used insecticide. The metabolite of chlorpyrifos, chlorpyrifos oxon, is a potent irreversible inhibitor of acetylcholinesterase, much like the nerve agent Sarin. To date, the target brain region(s) most susceptible to the neuroactive effects of chlorpyrifos oxon have yet to be identified. To address this we tested ability of chlorpyrifos oxon to influence neuronal excitability and induce lasting changes in the locus coeruleus, a brain region implicated in anxiety, substance...
Tipo: Manuscript
Palavras-chave: Neuroscience; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/6057/version/2

Reduced GABA-B/GIRK-mediated regulation of the VTA following a single exposure to cocaine
Provedor de dados: Nature Precedings
Autores: Brian Cadle; Andrew Varnell; Donald C. Cooper.
In this paper, Arora and colleagues expand on their previous work on GIRK channels in the ventral tegmental area (VTA) presenting evidence that a single exposure to cocaine reduces inhibitory GABAergic transmission to dopamine (DA) neurons in the ventral tegmental area. Mice receiving i.p. injections of cocaine saw a short lived (1-5 days) decrease in GABAb mediated G-protein coupled inwardly-rectifying potassium (GIRK) currents in DA neurons in the VTA. This decrease parallels an NMDA-mediated increase in the frequency of glutamatergic neurotransmission. Chronic cocaine injections had no additional effects beyond those seen with single injections. Though they found no change in mRNA levels for GABAb receptors, GIRK channels, or RGS-2 (a G-protein...
Tipo: Manuscript
Palavras-chave: Neuroscience; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6771/version/1

mGluR5 knockout mice exhibit normal conditioned place-preference to cocaine
Provedor de dados: Nature Precedings
Autores: Melissa  A. Fowler; Andrew L. Varnell; Donald C. Cooper.
Metabotropic glutamate receptor 5 (mGluR5) null mutant (-/-) mice have been reported to totally lack the reinforcing or locomotor stimulating effects of cocaine. We tested mGluR5 -/- and +/+ mice for their locomotor and conditioned place- preference response to cocaine. Unlike the previous finding, here we show that compared to mGluR5 +/+ mice, -/- mice exhibit no difference in the locomotor response to low to moderate doses of cocaine (10 or 20 mg/kg). A high dose of cocaine (40 mg/kg) resulted in a blunted rather than absent locomotor response. We tested mGluR5 -/- and +/+ mice for conditioned place-preference to cocaine and found no group differences at a conditioning dose of 10 mg/kg, suggesting normal conditioned rewarding properties of cocaine. These...
Tipo: Manuscript
Palavras-chave: Neuroscience; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/6180/version/2

Gulf War Syndrome: A role for organophosphate induced plasticity of locus coeruleus neurons
Provedor de dados: Nature Precedings
Autores: Jun-li  Cao; Andrew L. Varnell; Donald C. Cooper.
Gulf War syndrome is a chronic multi-symptom illness that has affected about a quarter of the deployed veterans of the 1991 Gulf War. Exposure to prolonged low-level organophosphate insecticides and other toxic chemicals is now thought to be responsible. Chlorpyrifos was one commonly used insecticide. The metabolite of chlorpyrifos, chlorpyrifos oxon, is a potent irreversible inhibitor of acetylcholinesterase, much like the nerve agent Sarin. To date, the target brain region(s) most susceptible to the neuroactive effects of chlorpyrifos oxon have yet to be identified. To address this we tested ability of chlorpyrifos oxon to influence neuronal excitability and induce lasting changes in the locus coeruleus, a brain region implicated in anxiety, substance...
Tipo: Manuscript
Palavras-chave: Neuroscience; Pharmacology.
Ano: 2011
URL: http://precedings.nature.com/documents/6057/version/1

Deletion of the trpc4 gene and its role in simple and complex strategic learning
Provedor de dados: Nature Precedings
Autores: William  D. Klipec; Phuong  Nguyen; Bridget  Deeney; Claire  Williamson; Kami  Wenzel; Jessica  Stumme; Marissa  Collins; Abby  Drish; Laura  Swenson; Eric  Ostertag; Donald C. Cooper.
The TRPC4 ion channel is expressed extensively in corticolimbic and a subpopulation of midbrain dopamine neurons. While TRPC4 knockout (KO) rats exhibit reduced sociability and social exploration, little is known about the role of TRPC4 in motivation and learning. To identify a function for TRPC4 channels in learning processes &amp;#xa0;we tested TRPC4 KO and normal&amp;#xa0;wild type (WT) rats. TRPC4 KO and WT rats exhibited no differences in Y-&amp;#xad;maze&amp;#xa0;learning or simple discrimination learning. Furthermore, on a more complex serial reversal shift task designed &amp;#xa0;to assess strategic learning where the reward and non-&amp;#xad;reward cues were repeatedly reversed between training sessions both TRPC4 KO and WT rats &amp;#xa0;...
Tipo: Manuscript
Palavras-chave: Neuroscience; Pharmacology.
Ano: 2012
URL: http://precedings.nature.com/documents/6929/version/1