Consulta: creatorFacets:"Dubos, Marie-pierre"
Registros recuperados: 2
Data/hora: 17/06/2024 06:57:15

Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas
Provedor de dados: 5
Autores: Bigot, Laetitia; Beets, Isabel; Dubos, Marie-pierre; Boudry, Pierre; Schoofs, Liliane; Favrel, Pascal.
Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (CgsNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values...
Tipo: Text
Palavras-chave: Oyster; Crassostrea gigas; SNPF; Energy metabolism; Reproduction; Starvation.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00207/31777/30188.pdf

Gametogenesis in the Pacific Oyster Crassostrea gigas: A Microarrays-Based Analysis Identifies Sex and Stage Specific Genes
Provedor de dados: 5
Autores: Dheilly, Nolwenn; Lelong, Christophe; Huvet, Arnaud; Kellner, Kristell; Dubos, Marie-pierre; Riviere, Guillaume; Boudry, Pierre; Favrel, Pascal.
Background: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. Methodology/Principal Findings: Gene expression was...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00087/19862/17505.pdf