Consulta: creatorFacets:"Dumay, Justine"
Registros recuperados: 10
Data/hora: 05/06/2024 18:12:15

Ultrasound-assisted extraction of R-phycoerythrin from Grateloupia turuturu with and without enzyme addition
Provedor de dados: 5
Autores: Le Guillard, Cecile; Dumay, Justine; Donnay-moreno, Claire; Bruzac, Sandrine; Ragon, Jean-yves; Fleurence, Joel; Berge, Jean-pascal.
The aim of this study was to compare two processes for the extraction of R-phycoerythrin (R-PE) from the red seaweed Grateloupia turuturu: ultrasound-assisted extraction (UAE) and ultrasound-assisted enzymatic hydrolysis (UAEH). Process efficiencies were both evaluated by the yield of R-PE extraction and by the level of liquefaction. Experiments were conducted at 40 and 22 °C, for 6 h, using an enzymatic cocktail and an original ultrasonic flow-through reactor. R-PE appeared very sensitive to temperature, thus 22 °C is strongly recommended for its extraction by UAEH or UAE. However, the higher processing temperature (40 °C) clearly increased the extraction of water-soluble compounds (up to 91% of liquefaction).  These two new processes are thus promising...
Tipo: Text
Palavras-chave: Seaweed; Grateloupia turuturu; R-phycoerythrin; Ultrasound-assisted extraction; Ultrasound-assisted enzymatic hydrolysis; Liquefaction.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00292/40332/39993.pdf

Valorization of the Macroalgae Sargassum Muticum by Enzymatic Hydrolysis, Interest of Surfactants to Improve the Extraction of Phlorotannins and Polysaccharides
Provedor de dados: 5
Autores: Vandanjon, Laurent; Vallet, Laurent; Le Glatin, Titouan; Deleris, Paul; Baron, Regis; Bourseau, Patrick; Dumay, Justine.
The use of surfactants to improve enzymatic hydrolysis of the macroalgae Sargassum muticum has been investigated. Visible absorption spectroscopy has been used to quantify the solubilization of both polysaccharides and phlorotannins in the hydrolysates.   After total extraction, results showed that Sargassum muticum contained 2.74% (expressed in percent of the dry weight of the algae) of phlorotannins whose 32 % were in the cell wall. This result shows that it is important to access to the parietal phlorotannins. To reach this objective, we chose the enzymatic approach for destructurating the cell wall of the algae. The use of 5% dry weight (DW - 5% by weight of hydrolyzed algae) of an enzymatic mix containing a commercial beta-glucanase, a commercial...
Tipo: Text
Palavras-chave: Brown macroalgae; Sargassum muticum; Enzymatic hydrolysis; Surfactants; Phlorotannins.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00350/46105/46073.pdf

Development of lipid oxidation during manufacturing of horse mackerel surimi
Provedor de dados: 5
Autores: Eymard, Sylvie; Carcouet, Elodie; Rochet, Marie-joelle; Dumay, Justine; Chopin, Christine; Genot, Claude.
When fatty fish are transformed into surimi, lipid oxidation takes place, decreasing the quality of the product. This study was aimed to identify the critical stages of the process in terms of the development of lipid oxidation. Horse mackerels were transformed into surimi on a pilot line and samples taken (hand-skinned fillets = minced fillets, mince, washed and refined minces, paste, surimi and washing water). Most of the lipids were removed during the process and neutral lipids were lost in higher proportion than polar lipids. As a consequence, total lipids of surimi contained more polyunsaturated fatty acids (338 19 g kg(-1)) than total lipids of the minced fillets (220 8 g kg-1). Thiobarbituric acid reactive substances (TBARS) was higher in the minced...
Tipo: Text
Palavras-chave: Process; Fatty fish; Surimi; Polyunsaturated fatty acids; Lipid oxidation.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-424.pdf

Optimization of hydrolysis of sardine (Sardina pilchardus) heads with Protamex: enhancement of lipid and phospholipid extraction
Provedor de dados: 5
Autores: Dumay, Justine; Allery, Marion; Donnay-moreno, Claire; Barnathan, Gilles; Jaouen, Pascal; Carbonneau, Marie Elise; Berge, Jean-pascal.
BACKGROUND: Fish by-products are not considered as valuable raw materials even if they usually contain valuable components such as lipids. Fish lipids are well known for their nutritional potential and health effects but their extraction remains problematic due to the use of organic solvents. Enzymatic hydrolysis such as the proteolysis of tissues can lead to lipid extraction. RESULTS: Hydrolysis of sardine heads by Protamex was studied (temperature, hydrolysis time and enzyme-substrate ratio) using response surface methodology in order to obtain the highest release of lipids and particularly phospholipids. No relation between the degree of hydrolysis and lipid recovery were depicted; however, optimum conditions for both the release of lipids and...
Tipo: Text
Palavras-chave: Response surface methodology; Proteolysis; Sardine; By product; Fatty acids; Phospholipids.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6651.pdf

Improvement of lipid and phospholipid recoveries from sardine (Sardina pilchardus) viscera using industrial proteases
Provedor de dados: 5
Autores: Dumay, Justine; Donnay-moreno, Claire; Barnathan, G; Jaouen, P; Berge, Jean-pascal.
Enzymatic hydrolysis of sardine viscera by three broad spectrum proteases was investigated using the pHstat method (24 h, pH 8, 50 degrees C). After hydrolysis, three fractions (sludge, aqueous phase and oily phase) were collected. For each fraction, lipids and phospholipids were quantified and molecular weights of aqueous phase peptides were determined. Under these conditions, the degree of hydrolysis (DH) varied from 1.9% (Flavourzyme), 3.1% (Protamex) and to 3.3% (Alcalase). Dry matter distribution indicated that all hydrolysis performed increased solubilisation of proteins: protein recovery for each hydrolysis ranged from 57.4% to 61.2%. Furthermore, the total lipid content in the liquid parts (oily and aqueous phases) increased (at least 85% of lipids...
Tipo: Text
Palavras-chave: Fish protein hydrolysate FPH; Sardine; By product; Lipid; Hydrolysis; Phospholipid.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2004.pdf

Seasonal variation in the antivibrio activity of two organic extracts from two red seaweed: Palmaria palmata and the introduced Grateloupia turuturu against the abalone pathogen Vibrio harveyi
Provedor de dados: 5
Autores: Garcia-bueno, Nuria; Dumay, Justine; Guerin, Thomas; Turpin, Vincent; Paillard, Christine; Stiger-pouvreau, Valerie; Pouchus, Yves-francois; Aitor Marin-atucha, Arnaldo; Decottignies, Priscilla; Fleurence, Joel.
The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts.
Tipo: Text
Palavras-chave: Antibacterial activity; Abalone disease; Haliotis tuberculata; Red seaweed; Vibrio harveyi.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00617/72875/71929.pdf

Extraction de lipides en voie aqueuse par bioréacteur enzymatique combine à l'ultrafiltration : application a la valorisation de co-produits de poisson (Sardina pilchardus)
Provedor de dados: 5
Autores: Dumay, Justine.
The main goal of this work was to up-grade sardine (Sardina pilchardus) by-products using mild procedure, environmentally sound, in order to obtain valuable compounds involving moderate investment and low energy consumption.   Hydrolysis and ultrafiltration techniques have been investigated. Firstly, selected enzymes have been calibrated in order to determine their optimal conditions with a model substrate and to permit the comparison between them. Then, hydrolysis on sardine head and viscera have been carried out in two steps. The first one had the objective to determine the enzyme efficiency and the study area. The second step was to optimise enzymatic hydrolysis using experimental designs. The aim of this second step was to obtain the highest lipid...
Tipo: Text
Palavras-chave: Up grading; By products; Ultrafiltration; Enzymes; Lipids; Sardina pilchardus; Valorisation; Co produits; Ultrafiltration; Enzymes; Lipides; Sardina pilchardus.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/these-1556.pdf

COSELMAR. Compréhension des socio-écosystèmes littoraux et marins pour la prévention et la gestion des risques. Bilan scientifique 2013-2017
Provedor de dados: 5
Autores: Pardo, Sophie; Hess, Philipp; Simon, Elodie; Barille, Laurent; Geslin, Emmanuelle; Cognie, Bruno; Martin-jezequel, Véronique; Sechet, Véronique; Herrenknecht, Christine; Baron, Regis; Bourseau, Patrick; Amzil, Zouher; Masse, Anthony; Vandanjon, Laurent; Dumay, Justine; Lebeau, Thierry; Turpin, Vincent; Mondeguer, Florence; Petitgas, Pierre; Trouillet, Brice; Mercier, Denis; Guillotreau, Patrice; Guineberteau, Thierry; Mahevas, Stephanie; Schoefs, Franck; Sophie Pardo; Philipp Hess; Pauline Borgniet; Laurent Barillé; Emmanuelle Geslin; Bruno Cognie; Véronique Martin-Jezequel; Véronique Sechet; Christine Herrenknecht; Régis Baron; Patrick Bourseau; Zouher Amzil; Anthony Massé; Laurent Vandanjon; Justine Dumay; Thierry Lebeau; Vincent Turpin; Florence Mondeguer; Pierre Petitgas; Brice Trouillet.
COSELMAR, a 4-year project financed by the Région des Pays de la Loire at 2.1 M€, officially started on the 7th January 2013. The project is coordinated by Philipp Hess (IFREMER) and Sophie Pardo (Université de Nantes-LEMNA) and falls under the Federation for Research Institut Universitaire Mer et Littoral (IUML, FR CNRS 3473)*. COSELMAR has also been approved by the Scientific Council of the MSH Ange Guépin. COSELMAR is a research project uniting 5 research units of IFREMER and 11 laboratories of the Université de Nantes, along with academic partners, and national and international industries. The aim is to achieve a better understanding of the marine and coastal ecosystems and the associated resources. The project will also provide insights into risk...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00406/51793/52550.pdf

Recovery of valuable soluble compounds from washing waters generated during small fatty pelagic surimi processing by membrane processes
Provedor de dados: 5
Autores: Dumay, Justine; Radier, Severine; Barnathan, Gilles; Berge, Jean-pascal; Jaouen, Pascal.
This work focuses on the treatment of washing waters coming from surimi manufacturing using ultrafiltration technology at a laboratory scale. Four membrane materials (poly-ether sulfone, polyacrilonytrile, poly vinylidene fluoride and regenerated cellulose) and 5 Molecular Weight Cut-Off (from 3 to 100 kDa) have been studied at bench laboratory scale using the pilot Rayflow (R) 100, commercialised by Rhodia Orelis. The investigation deals with the ability for membranes to offer a high retention of biochemical compounds (proteins and lipids). Results obtained during adsorption tests showed that the regenerated cellulose material seems to be the most appropriate with regards to pore size reduction due to the protein-adsorption. During the ultrafiltration of...
Tipo: Text
Palavras-chave: Fish oil; Regenerated cellulose; Surimi wastewater; Enzymatic hydrolysis; Ultrafiltration.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4359.pdf

Soft liquefaction of the red seaweed Grateloupia turuturu Yamada by ultrasound-assisted enzymatic hydrolysis process
Provedor de dados: 5
Autores: Le Guillard, Cecile; Berge, Jean-pascal; Donnay-moreno, Claire; Bruzac, Sandrine; Ragon, Jean-yves; Baron, Regis; Fleurence, Joel; Dumay, Justine.
Ultrasound-assisted enzymatic hydrolysis is a recent process, increasingly employed for plant biomass liquefaction and the recovery of soluble biomolecules. However, to our knowledge, it has never been used on seaweeds, particularly wet ones. The aim of this study was to compare the efficiency of three processes on the liquefaction of the red seaweed Grateloupia turuturu Yamada: enzyme-assisted extraction (EAE), ultrasound-assisted extraction (UAE), and their combination, ultrasound-assisted enzymatic hydrolysis (UAEH). These comparisons will allow the identification as to which process achieves the highest extraction yield of water-soluble compounds. For this purpose, experiments were conducted at 40 °C for 6 h using an enzymatic cocktail of four...
Tipo: Text
Palavras-chave: Ultrasound-assisted enzymatic hydrolysis; Enzymatic hydrolysis; Ultrasound; Liquefaction; Algae; Grateloupia turuturu.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00308/41877/44316.pdf