Consulta: creatorFacets:"Falk,Torsten"
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Data/hora: 09/06/2024 17:39:06

A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture
Provedor de dados: 69
Autores: Falk,Torsten; Strazdas,Lori A.; Borders,Rebecca S.; Kilani,Ramsey K.; Yool,Andrea J.; Sherman,Scott J.
High-density cultures of mammalian neurons offer a model system for studies of brain development, but the morphological features of individual neurons is difficult to ascertain. We show that a herpes virus vector expressing a bioluminescent protein allows detailed morphometric analyses of living neurons in complex culture environments. Expression of enhanced green fluorescent protein (eGFP) was constitutively driven in neurons using the herpes simplex virus amplicon system. This system allowed us to make novel observations regarding development in high-density cultures from rat hippocampus and cerebellum. After the phase of initial neurite outgrowth, maturing neurons continue to show rapid remodeling of the neurite branches (0.79 ± 0.11 mum/h per neurite;...
Tipo: Journal article
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100004