Consulta: creatorFacets:"Farcy, Emilie"
Registros recuperados: 4
Data/hora: 10/06/2024 06:48:53

Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Masroor, Waliullah; Farcy, Emilie; Blondeau-bidet, Eva; Venn, Alexander; Tambutté, Eric; Lorin-nebel, Catherine.
The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 °C (temperate) or 24 °C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase α1a (nka α1a) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl−-cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters...
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Ion transporter; Gill; MRNA expression; Thermal acclimation; Salinity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00514/62600/66958.pdf

Inter-individual variability in freshwater tolerance is related to transcript level differences in gill and posterior kidney of European sea bass
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: L'Honoré, Thibault; Farcy, Emilie; Blondeau-bidet, Eva; Lorin-nebel, Catherine.
Acclimation to low salinities is a vital physiological challenge for euryhaline fish as the European sea bass Dicentrarchus labrax. This species undertakes seasonal migrations towards lagoons and estuaries where a wide range of salinity variations occur along the year. We have previously reported intraspecific differences in freshwater tolerance, with an average 30% mortality rate. In this study, we bring new evidence of mechanisms underlying freshwater tolerance in sea bass at gill and kidney levels. In fresh water (FW), intraspecific differences in mRNA expression levels of several ion transporters and prolactin receptors were measured. We showed that the branchial Cl-/HCO3- anion transporter (slc26a6c) was over-expressed in freshwater intolerant fish,...
Tipo: Text
Palavras-chave: Intraspecific variation; Osmoregulation; Freshwater acclimation; Ion transporters; SLC26A6; NKCC; PRLR.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00614/72569/71524.pdf

Seasonal changes in mRNA encoding for cell stress markers in the oyster Crassostrea gigas exposed to radioactive discharges in their natural environment
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Farcy, Emilie; Voiseux, Claire; Lebel, Jean-marc; Fievet, Bruno.
The North Cotentin area (Normandy, France) hosts several nuclear facilities among which the AREVA reprocessing plant of La Hague is responsible for controlled discharges of liquid radioactive wastes into the marine environment. The resulting increase in radioactivity is very small compared to natural radioactivity. However, concerns about environment protection prompted the scientific community to focus on the effects of the chronic exposure to low concentrations of radionuclides in non-human biota. This study contributes to the evaluation of the possible impact of radioactive discharges on the oyster Crassostrea gigas in the field. Real-time polymerase chain reaction was used to quantify the expression levels of genes involved in cell stress in the...
Tipo: Text
Palavras-chave: Crassostrea gigas; Superoxide dismutase; Metallothionein; Heat shock protein; MRNA; Seasonal variations; Radioactive discharges.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2601.pdf

Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function?
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rivera-ingraham, Georgina A.; Barri, Kiam; Boel, Melanie; Farcy, Emilie; Charles, Anne-laure; Geny, Bernard; Lignot, Jehan-herve.
Osmoregulating decapods such as the Mediterranean green crab Carcinus aestuarii possess two groups of spatially segregated gills: anterior gills serve mainly respiratory purposes, while posterior gills contain osmoregulatory structures. The co-existence of similar tissues serving different functions allows the study of differential adaptation, in terms of free radical metabolism, upon salinity change. Crabs were immersed for 2 weeks in seawater (SW, 37 ppt), diluted SW (dSW, 10 ppt) and concentrated SW (cSW, 45 ppt). Exposure to dSW was the most challenging condition, elevating respiration rates of whole animals and free radical formation in hemolymph (assessed fluorometrically using C-H(2)DFFDA). Further analyses considered anterior and posterior gills...
Tipo: Text
Palavras-chave: Antioxidant defense; Carcinus aestuarii; ROS production; Osmoregulation.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00626/73816/74410.pdf