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Effect of freezing on rabbit cultured chondrocytes
Provedor de dados: 45
Autores: Filgueiras,R.R; Del Carlo,R.J; Alves,N.P; Viloria,M.I.V; McManus,C.M; Castro,M.B; Filgueiras,F.P.F; Monteiro,B.S; Paula,A.C; Farias,A.
This work evaluated the effect of freezing on chondrocytes maintained in culture, aiming the establishment of a cell bank for future application as heterologous implant. Chondrocytes extracted from joint cartilage of nine healthy New Zealand White rabbits were cultivated and frozen with the cryoprotector 5% dimethylsulfoxide for six months. Phenotypic and scanning electron microscopy analyses were carried out to identify morphological and functional differences between fresh and thawed cells. After enzymatic digestion, a total of 4.8x10(5)cells per rabbit were obtained. Fresh chondrocytes showed a high mitotic rate and abundant matrix was present up to 60 days of culture. Loss of phenotypic stability was notable in the thawed chondrocytes, with a low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabbit; Cell freezing; Cell therapy; Joint cartilage; Type II collagen.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100008