Consulta: creatorFacets:"Fiuza,Lidia Mariana"
Registros recuperados: 15
Data/hora: 09/06/2024 05:37:32

Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae)
Provedor de dados: Revista Brasileira de Entomologia
Autores: Franz,Andréa Roveré; Knaak,Neiva; Fiuza,Lidia Mariana.
Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae). Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf), Zingiber officinale (root) and Mentha sp. (leaf). The efficacy of these oils was tested to control the rice weevil, S. oryzae, using hydrodistillation. Chemical analysis of the essential oils was carried out by gas chromatography. Major components of C. citratus were geranial (48%) and neral (31%), of Z. officinale were α-zingibereno...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Stored product pests; Botanical insecticides.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262011000100018

Frequência de Bacillus spp. em solos de diferentes sistemas de cultivo de arroz irrigado em Cachoeirinha, RS
Provedor de dados: Bragantia
Autores: Fritz,Leila Lucia; Berlitz,Diouneia Lisiane; Macedo,Vera Regina Mussoi; Machado,Vilmar; Fiuza,Lidia Mariana.
Bacilos entomopatogênicos são bactérias observadas principalmente no solo, consideradas importantes agentes no controle biológico de doenças e pragas da agricultura. No entanto, existem poucos estudos que relatam a influência das práticas agrícolas nas populações de Bacillus spp. Essa pesquisa objetivou avaliar a freqüência de bactérias entomopatogênicas pertencentes ao gênero Bacillus obtidas de amostras de solo coletadas no ano agrícola de 2006/2007 de diferentes sistemas de cultivo de arroz irrigado da EEA-IRGA, Cachoeirinha (RS). Foram analisadas 36 amostras de solos, oriundas de três sistemas de plantio de arroz irrigado, pré-germinado (SPG), convencional (SPC), plantio direto (SPD) e de uma área sem plantio de arroz (C). Para tanto, um grama de solo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agroecossistemas; Bacilos entomopatogênicos; Sistemas de plantio; Bacillus thuringiensis; Bacillus sphaericus; Bacillus cereus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000200020

Microbial Diversity: Relevance and Relationship Between Environmental Conservation And Human Health
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Panizzon,Jeremias Pakulski; Pilz Júnior,Harry Luiz; Knaak,Neiva; Ramos,Renata Cristina; Ziegler,Denize Righetto; Fiuza,Lidia Mariana.
This work presents bibliographic data on the role and function of microbial diversity. The increasing use of probiotics and prebiotics foods has led to the studies on their actual functions in the human body. It is known that in the environment, microorganisms are extremely important in recycling of nutrients, balance of trophic chains, vital physiological activities in the plants and animals, as well as the conservation of natural habitats. In human food, these microscopic organisms contribute from flavoring products to the synthesis of antimicrobial substances and vitamins essential to living beings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Nutraceutical; Collective health; Review.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100137

Benefits Associated with the Interaction of Endophytic Bacteria and Plants
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Santos,Miriam Langner dos; Berlitz,Diouneia Lisiane; Wiest,Shana Leticia Felice; Schünemann,Rogerio; Knaak,Neiva; Fiuza,Lidia Mariana.
ABSTRACT The endophytic bacteria belong to a larger group of microorganisms that have their life-cycle partly or entirely inside the plant and are located in intra and inter-cellular spaces or in the vascular tissue. These bacteria can be found colonizing aerial parts or roots. This review aims to analyze the colonization strategies of endophytic bacteria through interaction with plants, as well as to highlight the metabolic influence of these organisms in plant tissues, which result in physiological and biochemical changes. Depending on the different mechanisms used internally to colonize a plant, these microorganisms are called obligate, facultative, or passive endophytes. Phytostimulation, biofertilization and biological control are mechanisms that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofertilization; Biological control; Colonization strategies; Microorganisms; Phytostimulation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100200

Mortality of Oryzophagus oryzae (Costa Lima, 1936) (Coleoptera: Curculionidae) and Spodoptera frugiperda (J E Smith, 1797) (Lepidoptera: Noctuidae) Larvae Exposed to Bacillus thuringiensis and Extracts of Melia azedarach
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Berlitz,Diouneia Lisiane; Azambuja,Aline Oliboni de; Sebben,Alessandra; Oliveira,Jaime Vargas de; Fiuza,Lidia Mariana.
Oryzophagus oryzae (Costa Lima 1936) (Coleoptera: Curculionidae) and Spodoptera frugiperda (J E Smith, 1797) (Lepidoptera: Noctuidae) cause important crop losses in southern Brazil. Control is possible by the use of the bacteria Bacillus thuringiensis and extracts of Melia azedarach. This study aimed to evaluate the mortality, in vivo, of O. oryzae and S. frugiperda submitted to two isolates of B. thuringiensis and the aqueous extract of M. azedarach. The LC50 for O. oryzae due to bacteria was 5.40μg/mL (Bt 2014-2) and due to plant extract 0.90μg/mL. For S. frugiperda, the Bt 1958-2 bacterial suspension (1.10(10)UFC/mL) caused a 100% of corrected mortality, showing that the purified Cry proteins caused a CL10 of 268μg/mL five days after the treatments, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative pest control; Entomopathogenic bacteria; Insecticidal activity; Botanical insecticidal.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500012

Functional Genes of Microorganisms, Comprehending the Dynamics of Agricultural Ecosystems
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Reali,Catiusca; Pilz Júnior,Harry Luiz; Meile,Jean Christophe; Galindo,Sabine Shorr; Fiuza,Lidia Mariana.
ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to maintain the sustainability of a crop. Molecular tools to study microbial communities are possible through many methods such as RISA, DGGE, TGGE, clone libraries, T-RFLP, RAPD, SSCP and more recently NGS (Next-Generation Sequencing). DGGE is widely employed to characterize the diversity and the community dynamics of microorganisms in the environment, making possible to find out specific groups through functional genes, allowing access to data that cannot be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agroecosystems; Functional genes; Bacteria; DGGE; Metagenomics; Microbial ecology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100201

PCR and bioassays screening of Bacillus thuringiensis isolates from rice-fields of Rio Grande do Sul, specific to lepidopterans and coleopterans
Provedor de dados: Brazilian Journal of Microbiology
Autores: Pinto,Laura Massochin Nunes; Fiuza,Lidia Mariana.
Bacillus thuringiensis (Bt) isolates from soil samples of rice-fields in Rio Grande do Sul (RS) were tested through PCR, aiming at the screening of six groups of Bt cry genes, which codify active proteins for coleopterans and lepidopterans rice pests, and their bioinsecticide potential regarding their use in IPM system, as well. Forty six Bt isolates were grown in Agar Nutrient for 12 h and submitted to total DNA extraction. The amplified fragments were analyzed in agarose gel (1-1.5%). The screening isolates showed that 56.51% were potentially lepidopterans specific (cry1, cry2 and cry9) and 21.73% were coleopteran specific (cry3 and cry7/8), with a homogeneous distribution in the rice-field areas in Rio Grande do Sul. Cry2 genes were found just once and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus thuringiensis; PCR; Bioassay; Lepidoptera; Coleoptera.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400003

Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Aanticarsia gemmatalis (Lepidoptera:Noctuidae)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Bobrowski,Vera Lucia; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Fiuza,Lidia Mariana.
The bacterium Bacillus thuringiensis (Bt) is characterized by its ability to produce proteic crystalline inclusions during sporulation. Cry1 protein has insecticidal activity and is highly specific to certain insects and not toxic to unrelated insects, plants or vertebrates. In this work, the patogenicity of twelve Bt isolates was tested against Anticarsia gemmatalis, one of the most important insect pests of soybeans. Spore-crystal complex was applied to the surface of artificial diets and the mortality of A. gemmatalis larvae was assessed seven days after each treatment. When compared to a control Bt isolate known by its high toxicity to A. gemmatalis larvae, four novel Bt isolates exhibited even higher toxic activities against the insect, resulting in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus thuringiensis; Velvetbean caterpillar; Anticarsia gemmatalis; Cry1 gene; Delta-endotoxin.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200006

Histopathology of Anticarsia gemmatalis Hübner (Lepidoptera; Noctuidae) treated with Nucleopolyhedrovirus and Bacillus thuringiensis serovar kurstaki
Provedor de dados: Brazilian Journal of Microbiology
Autores: Knaak,Neiva; Fiuza,Lidia Mariana.
The Anticarsia gemmatalis is responsible for the use of chemical insecticides in the soybean culture, causing a significant increase in the costs of farming and a great unbalance in the ecosystem. The use of microbial agents, like Bacillus thuringiensis serovar kurstaki (Btk) and Anticarsia gemmatalis nucleopolyhedrovirus (AgNPV), they are an alternative to chemical control of the pest insects. In the interaction analysis of the entomopathogenic bacteria and virus it is considered important the in vitro action mode of these microbiology control agents. Therefore, the present study aims the histopathological analysis of the A. gemmatalis larvae digestive system after the interaction in vivo of the entomopathogenic Btk and AgNPV, represented the Dipel and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Virus; Bacteria; Biological control; Lepidoptera.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200017

In vitro effect of Bacillus thuringiensis strains and Cry proteins in phytopathogenic fungi of paddy rice-field
Provedor de dados: Brazilian Journal of Microbiology
Autores: Knaak,Neiva; Rohr,Angelise Ana; Fiuza,Lidia Mariana.
Cry1Ab and Cry1Ac strains and proteins synthesized by Bacillus thuringiensis thuringiensis and B. thuringiensis kurstaki were assessed in the following phytopathogens: Rhizoctonia solani,Pyricularia grisea,Fusarium oxysporum and F. solani, which had their micelial growth decreased after incubation in the presence of the bacterial strains. As to Cry proteins, there were no inhibition halo development in the assessed concentrations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paddy rice; Bacillus thuringiensis; Phytopathogens; Cry proteins.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300027

Effectiveness of Bacillus thuringiensis strains against Spodoptera frugiperda (lepidoptera: noctuidae)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Polanczyk,Ricardo Antonio; Silva,Rogério Fernando Pires da; Fiuza,Lidia Mariana.
The fall armyworm (Spodoptera frugiperda) is one of the most important pests of maize, causing up to 20% production losses when defoliation occurs near to flowering, or even complete destruction of plants. Among the alternatives to control this pest, the use of Bacillus thuringiensis (Bt) has gained attention due to its efficiency and low impact on natural enemies. Strains Bt dendrolimus HD 37, Bt aizawai HD 68, Bt kurstaki HD 73, Bt darmstadiensis HD 146, and Bt thuringiensis 4412 were tested against second instar larvae in in vivo assays. Suspensions of Bt aizawai HD 68 and Bt thuringiensis 4412, containing 3 x 10(8) cells/ml, induced mortality of 100% and 80.4%, respectively. To test virulence, cell concentrations of 8 x 10(5) to 3 x 10(8) cells/ml of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus thuringiensis; Spodoptera frugiperda; Biological control; Bioassays; Fall armyworm.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300003

Genes de Bacillus thuringiensis: uma estratégia para conferir resistência a insetos em plantas
Provedor de dados: Ciência Rural
Autores: Bobrowski,Vera Lucia; Fiuza,Lidia Mariana; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena.
Os insetos constituem uma das principais causas de danos à produção agrícola no mundo. O controle de insetos tem sido realizado por meio de agroquímicos e, em muito menor escala, pelo emprego de inseticidas biológicos. As plantas transgênicas resistentes a insetos representam uma nova alternativa no combate aos insetos-praga das lavouras. A bactéria entomopatogênica Bacillus thuringiensis Berlinier (Bt) é a fonte dos genes de resistência nas chamadas plantas-Bt, produzidas comercialmente. No presente trabalho de revisão, são abordados os aspectos relacionados à bactéria Bt como fonte de genes de resistência a insetos-pragas, plantas geneticamente modificadas, vantagens do uso de plantas-Bt, bem como perspectivas dessa ferramenta biotecnológica.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus thuringiensis; Bt; Plantas transgênicas; Resistência a insetos; Insetos-praga; Delta-endotoxina.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000500008

Ocorrência de parasitóides de Spodoptera frugiperda (J. E. Smith)(Lep., Noctuidae) em lavouras de milho em Cachoeirinha, RS
Provedor de dados: Ciência Rural
Autores: Dequech,Sônia Thereza Bastos; Silva,Rogério Fernando Pires da; Fiuza,Lidia Mariana.
O Rio Grande do Sul é um dos maiores produtores de milho do país, porém pouco se conhece em termos de inimigos naturais dos principais insetos-praga da cultura. O objetivo do presente trabalho foi realizar um levantamento dos parasitóides da lagarta-do-cartucho do milho, Spodoptera frugiperda. Para tanto, foram efetuadas amostragens em áreas de lavouras de milho do Instituto Riograndense do Arroz (IRGA), em Cachoeirinha, RS, nos anos agrícolas 1999/2000 e 2000/2001. A coleta dos insetos foi realizada semanalmente, durante o ciclo da cultura. As lagartas foram individualizadas e mantidas em laboratório até a emergência dos adultos ou dos parasitóides. Foram coletadas 1425 lagartas no primeiro período de amostragens e 518 no segundo. Destas, 18,11% e 22,01%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campoletis flavicincta; Ichneumonidae; Braconidae; Tachinidae; Hiperparasitóides.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000400042

Histopatologia de lagartas de Spodoptera frugiperda (Lep., Noctuidae) infectadas por Bacillus thuringiensis aizawai e com ovos de Campoletis flavicincta (Hym., Ichneumonidae)
Provedor de dados: Ciência Rural
Autores: Dequech,Sônia Thereza Bastos; Fiuza,Lidia Mariana; Silva,Rogério Fernando Pires da; Zumba,Roberta Cristina.
Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera, Noctuidae) está entre os insetos-praga que mais danificam culturas, sendo possível seu controle com produtos à base de Bacillus thuringiensis Berliner, 1911 (Bt). Um dos principais parasitóides de S. frugiperda é Campoletis flavicincta (Ashmead, 1890) (Hymenoptera, Ichneumonidae). A interação de Bt aizawai e de C. flavicincta através da análise histopatológica do ovo do parasitóide em lagartas de S. frugiperda infectadas foi avaliada. Lotes de lagartas de segundo ínstar foram analisados: 1) parasitadas e infectadas por Bt aizawai (Xentari/Abbott Laboratórios do Brasil Ltda) a 0,5mg ml-1 e 2) apenas parasitadas por C. flavicincta (controle). Todas as lagartas foram fixadas em "Bouin Hollande", em...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lagarta-do-cartucho-do-milho; Controle microbiano; Inimigos naturais.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000100045

Distribuição de genes cry de Bacillus thuringiensis isolados de solos do Estado do Rio Grande do Sul, Brasil
Provedor de dados: Ciência Rural
Autores: Pinto,Laura Massochin Nunes; Fiuza,Lidia Mariana.
A bactéria Bacillus thuringiensis (Bt) caracteriza-se pela produção de proteínas tóxicas a representantes de diversas ordens de insetos, as quais são codificadas por genes cry. Devido a esta característica, mais de 40.000 cepas de Bt foram isoladas e cerca de 190 genes cry, caracterizados. Como os dados sobre Bt são limitados no Rio Grande do Sul, essa pesquisa objetivou avaliar a distribuição de seis famílias de genes cry de Bt, desse estado, que codificam proteínas ativas contra insetos-praga. O perfil dos 46 isolados de solos do Rio Grande do Sul foi avaliado, por PCR com os primers que detectam os genes cry1, cry2, cry3, cry7 cry8 e cry9 e suas respectivas proteínas foram analisadas por SDS-PAGE a 10%. A presença de genes cry9 foi detectada em 47,82%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Controle biológico; Entomopatógenos; Bactérias; PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000400018