Consulta: creatorFacets:"Fonteles,M.C."
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Data/hora: 16/06/2024 08:57:37

Effects of microcystin-LR in isolated perfused rat kidney
Provedor de dados: 56
Autores: Nobre,A.C.L.; Jorge,M.C.M.; Menezes,D.B.; Fonteles,M.C.; Monteiro,H.S.A.
Microcystin is a hepatotoxic peptide which inhibits protein phosphatase types 1 and 2A. The objective of the present study was to evaluate the physiopathologic effects of microcystin-LR in isolated perfused rat kidney. Adult Wistar rats (N = 5) of both sexes (240-280 g) were utilized. Microcystin-LR (1 µg/ml) was perfused over a period of 120 min, during which samples of urine and perfusate were collected at 10-min intervals to determine the levels of inulin, sodium, potassium and osmolality. We observed a significant increase in urinary flow with a peak effect at 90 min (control (C) = 0.20 ± 0.01 and treated (T) = 0.32 ± 0.01 ml g-1 min-1, P&amp;lt;0.05). At 90 min there was a significant increase in perfusate pressure (C = 129.7 ± 4.81 and T = 175.0 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcystin-LR; Kidney; Perfused kidney.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800008

Thalidomide and pentoxifylline block the renal effects of supernatants of macrophages activated with Crotalus durissus cascavella venom
Provedor de dados: 56
Autores: Martins,A.M.C.; Nobre,A.C.L.; Almeida,A.C.; Bezerra,G.; Lima,A.A.M.; Fonteles,M.C.; Monteiro,H.S.A.
Because thalidomide and pentoxifylline inhibit the synthesis and release of tumor necrosis factor-alpha (TNF-alpha), we determined the effect of these drugs on the renal damage induced by supernatants of macrophages activated with Crotalus durissus cascavella venom in order to identify the role of TNF-alpha in the process. Rat peritoneal macrophages were collected with RPMI medium and stimulated in vitro with C.d. cascavella venom (10 µg/ml) in the absence and presence of thalidomide (15 µM) or pentoxifylline (500 µM) for 1 h and washed and kept in culture for 2 h. Supernatant (1 ml) was tested on an isolated perfused rat kidney (N = 6 for each group). The first 30 min of each experiment were used as control. The supernatant was added to the perfusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nephrotoxicity; Crotalus durissus cascavella; Pentoxifylline; Thalidomide; Macrophage.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000012

Relationship between the actions of atrial natriuretic peptide (ANP), guanylin and uroguanylin on the isolated kidney
Provedor de dados: 56
Autores: Santos-Neto,M.S.; Carvalho,A.F.; Forte,L.R.; Fonteles,M.C.
Guanylin and uroguanylin are peptides that bind to and activate guanylate cyclase C and control salt and water transport in many epithelia in vertebrates, mimicking the action of several heat-stable bacteria enterotoxins. In the kidney, both of them have well-documented natriuretic and kaliuretic effects. Since atrial natriuretic peptide (ANP) also has a natriuretic effect mediated by cGMP, experiments were designed in the isolated perfused rat kidney to identify possible synergisms between ANP, guanylin and uroguanylin. Inulin was added to the perfusate and glomerular filtration rate (GFR) was determined at 10-min intervals. Sodium was also determined. Electrolyte dynamics were measured by the clearance formula. Guanylin (0.5 µg/ml, N = 12) or uroguanylin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ANP; Guanylin; Uroguanylin; Kidney; Natriuresis; CGMP.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800012

From Escherichia coli heat-stable enterotoxin to mammalian endogenous guanylin hormones
Provedor de dados: 56
Autores: Lima,A.A.M.; Fonteles,M.C.
The isolation of heat-stable enterotoxin (STa) from Escherichia coli and cholera toxin from Vibrio cholerae has increased our knowledge of specific mechanisms of action that could be used as pharmacological tools to understand the guanylyl cyclase-C and the adenylyl cyclase enzymatic systems. These discoveries have also been instrumental in increasing our understanding of the basic mechanisms that control the electrolyte and water balance in the gut, kidney, and urinary tracts under normal conditions and in disease. Herein, we review the evolution of genes of the guanylin family and STa genes from bacteria to fish and mammals. We also describe new developments and perspectives regarding these novel bacterial compounds and peptide hormones that act in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat-stable enterotoxin; Guanylin; Guanylyl cyclase; Secretory diarrhea; Kidney function; Electrolyte and water balance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300179

Expression of myo-inositol cotransporters in the sciatic nerve and dorsal root ganglia in experimental diabetes
Provedor de dados: 56
Autores: Farias,V.X.; Uchoa,P.N.; Aquino,C.P.; Britto,L.R.G.; Fonteles,M.C.; Leal-Cardoso,J.H.; Silva-Alves,K.S.; Havt,A.; Prata,M.M.G.; Heimark,D.B.; Nascimento,N.R.F.; Santos,C.F.
The transport of myo-inositol is the main mechanism for the maintenance of its high intracellular levels. We aimed to measure the mRNA and protein levels of myo-inositol cotransporters in the sciatic nerve (SN) and dorsal root ganglia (DRG) during experimental diabetes. Streptozotocin-induced (STZ; 4, 8, and 12 weeks; 65 mg/kg; ip) diabetic rats (DB) and age-matched euglycemic (E) rats were used for the analysis of mRNA and protein levels of sodium myo-inositol cotransporters 1, 2 (SMIT1, SMIT2) or H+/myo-inositol cotransporter (HMIT). There was a significant reduction in the mRNA levels for SMIT1 in the SN and DRG (by 36.9 and 31.0%) in the 4-week DB (DB4) group compared to the E group. SMIT2 was not expressed in SN. The mRNA level for SMIT2 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetic neuropathy; Inositol metabolism; Peripheral nervous tissues.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600609

Actions of Crotalus durissus terrificus venom and crotoxin on the isolated rat kidney
Provedor de dados: 56
Autores: Monteiro,H.S.A.; Silva,I.M.S.C. da; Martins,A.M.C.; Fonteles,M.C.
Many studies have reported the occurrence of lethal acute renal failure after snakebites. The aim of the present investigation was to determine alterations in renal function produced by Crotalus durissus terrificus venom and crotoxin as well as the histological alterations induced by these venoms. Isolated kidneys from Wistar rats weighing 240 to 280 g were perfused with Krebs-Henseleit solution containing 6 g% of previously dialyzed bovine serum albumin. The effects of Crotalus durissus terrificus venom and crotoxin were studied on glomerular filtration rate (GFR), urinary flow (UF), perfusion pressure (PP) and percentage sodium tubular transport (%TNa+). The infusion of Crotalus durissus terrificus venom (10 µg/ml) and crotoxin (10 µg/ml) increased GFR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus venom; Crotoxin; Kidney.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000017