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Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gomes,N.E.; Brunialti,M.K.C.; Mendes,M.E.; Freudenberg,M.; Galanos,C.; Salomão,R.
Lipopolysaccharide (LPS) activates neutrophils and monocytes, inducing a wide array of biological activities. LPS rough (R) and smooth (S) forms signal through Toll-like receptor 4 (TLR4), but differ in their requirement for CD14. Since the R-form LPS can interact with TLR4 independent of CD14 and the differential expression of CD14 on neutrophils and monocytes, we used the S-form LPS from Salmonella abortus equi and the R-form LPS from Salmonella minnesota mutants to evaluate LPS-induced activation of human neutrophils and monocytes in whole blood from healthy volunteers. Expression of cell surface receptors and reactive oxygen species (ROS) and nitric oxide (NO) generation were measured by flow cytometry in whole blood monocytes and neutrophils. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipopolysaccharide; Neutrophils; Monocytes; Cell surface receptors; Reactive oxygen species; Nitric oxide.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900007