Consulta: creatorFacets:"Galetti Junior,Pedro Manoel"
Registros recuperados: 16
Data/hora: 17/06/2024 01:21:51

Chromosome mapping of 5S rRNA genes differentiates Brazilian populations of Leporellus vittatus (Anostomidae, Characiformes)
Provedor de dados: 74
Autores: Aguilar,Cecilia Teixeira de; Galetti Junior,Pedro Manoel.
Among the anostomid fishes, the genus Leporellus is represented by only three species: L. nattereri, endemic of the Amazon River, L. retropinnis, endemic of the Piracicaba River, and L. vittatus, widely distributed in rivers from Peru, Colombia, Guianas, and different major hydrographic basins of Brazil. A cytogenetic study carried out on specimens of Leporellus vittatus from three major Brazilian hydrographic basins evidenced a karyotype of 54 metacentric and submetacentric chromosomes. C-banding analysis revealed the presence of large pericentromeric heterochromatic segments in all chromosomes and a telomeric block coincident with the NOR sites. Ag, CMA3 or MM staining, and FISH with ribosomal probes located the 45S ribosomal genes on the terminal region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-bands; 45S rDNA; 5S rDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200004

Amplification of a GC-rich heterochromatin in the freshwater fish Leporinus desmotes (Characiformes, Anostomidae)
Provedor de dados: 74
Autores: Margarido,Vladimir Pavan; Galetti Junior,Pedro Manoel.
This is the first description of the karyotype of Leporinus desmotes. The diploid female number was 2n = 54 meta- and submetacentric chromosomes. The nucleolar organizing regions (NORs) were studied by silver nitrate staining and rDNA fluorescence in situ hybridization (FISH) and were found to be located in the telomeric region of the long arm of the 9th pair. C-banding revealed centromeric and telomeric heterochromatin segments in most chromosomes. Intercalar blocks of heterochromatin were observed in the long arm of six chromosome pairs. Besides a NOR-adjacent heterochromatin, all of the intercalar heterochromatic segments were brightly fluorescent by mithramycin staining. These data suggest that a unique amplification of a primordial GC-rich...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300012

Heterochromatin patterns and karyotype relationships within and between the genera Brycon and Salminus (Pisces, Characidae)
Provedor de dados: 74
Autores: Margarido,Vladimir Pavan; Galetti Junior,Pedro Manoel.
Chromosomes of two Brycon species (B. lundii and B. microlepis) and Salminus hilarii were analyzed. Based on constitutive heterochromatin distribution patterns, karyotypic relationships within and between Bryconinae and Salmininae were examined. A monophyletic origin for the genus Brycon, comprising at least two chromosome synapomorphies (presence of two large submetacentric bearing paracentromeric and telomeric heterochromatin), is suggested. Moreover, Bryconinae and Salmininae may represent a monophyletic unit among Characidae, as they share several chromosome features.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300012

Editorial
Provedor de dados: 74
Autores: Foresti,Fausto; Galetti Junior,Pedro Manoel.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200001

Heterochromatin analysis in the fish species Liposarcus anisitsi (siluriformes) and Leporinus elongatus (characiformes)
Provedor de dados: 74
Autores: Artoni,Roberto Ferreira; Molina,Wagner Franco; Bertollo,Luis Antonio Carlos; Galetti Junior,Pedro Manoel.
The chromosomes of two neotropical freshwater fish species, namely Liposarcus anisitsi (Siluriformes, Loricariidae) and Leporinus elongatus (Characiformes, Anostomidae), were investigated by means of C-banding, Ag-NORs, fluorochrome staining and banding by hot saline solution (HSS) treatment, to reveal patterns of heterochromatin differentiation. The karyotype of L. anisitsi is described for the first time. Staining with the GC-specific fluorescent antibiotic mithramycin (MM) revealed bright signals in some C-banded blocks in both species, suggesting that these MM+ heterochromatin contains GC-rich DNA. Banding by denaturation employing HSS, followed by Giemsa staining, yielded corresponding results documenting the thermal stability of GC-rich DNA part of...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100009

First karyotypical description of two American Ciconiiform birds, Mycteria americana (Ciconiidae) and Platalea ajaja (Threskiornithidae) and its significance for the chromosome evolutionary and biological conservation approaches
Provedor de dados: 74
Autores: Francisco,Mercival Roberto; Galetti Junior,Pedro Manoel.
The karyotypes of Mycteria americana (Ciconiidae) and Platalea ajaja (Threskiornithidae) are described. Both species have a diploid number of 2n = 72. There are slight chromosome morphology differences, which could be related to distinct chromosome evolution pathways of these two families. Besides a better understanding of the chromosome relationships among Ciconiiformes, this first chromosome characterization of M. americana and P. ajaja is an important tool for the conservation of both species.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400015

In silico analysis of polymorphic microsatellites in penaeid shrimp and construction of a free-access database
Provedor de dados: 74
Autores: Freitas,Patrícia Domingues de; Pinheiro,Allysson Pontes; Silva,Thiago Buosi; Galetti Junior,Pedro Manoel.
We performed an in silico analysis of all microsatellites so far described for penaeid shrimp and for which the polymorphic behavior has previously been analyzed. The objective of the study was to evaluate the structural characteristics of these microsatellites and identifying patterns which allow the characterization of the nature of these sequences in the penaeid genome. All data were compiled in a free-access database specially constructed for this study. Three hundred non-mononucleotide polymorphic microsatellite loci described for 12 shrimp species belonging to the family Penaeidae were analyzed and simple and compound microsatellites with di-, tri-, tetra-, penta- and hexanucleotide motifs were found. Dinucleotides and trinucleotides were the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacea; Microsatellite structure; Penaeidae; SSR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600027

Informative microsatellites for genetic population studies of black-faced lion tamarins (Leontopithecus caissara)
Provedor de dados: 74
Autores: Martins,Milene Moura; Galetti Junior,Pedro Manoel.
Leontopithecus caissara is a critically endangered primate species from the Brazilian Atlantic Forest. Nineteen microsatellite loci, previously developed for congeneric species, were tested with 34 L. caissara individuals from Superagüi Island. Of the 19 loci, 17 (89.4%) produced robust alleles, nine (47.4%) of these proved to be polymorphic, with a total of 23 alleles and an average of 2.56 alleles per locus. Expected and observed heterozygosity averaged 0.483 and 0.561, respectively. The exclusion power for identifying the first parent of an arbitrary offspring was 0.315 over all loci. The results thus indicate both the usefulness and limitations of these nine microsatellite loci in the genetic analysis of L. caissara, as well as their potentiality for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lion tamarins; Endangered species; Genetic diversity; New World primate; SSR transferability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100030

Heterochromatin heterogeneity and chromosome heteromorphism in Cerdocyon thous (Mammalia, Canidae)
Provedor de dados: 74
Autores: Hatanaka,Terumi; Tambasco,Antonio Junqueira; Galetti Junior,Pedro Manoel.
Few cytogenetic studies have been conducted on South American canids. Cerdocyon thous is a representative of the Canidae, living in the forests, open wooded areas and savannahs of South America. Compared to other canid species, C. thous has a large proportion of metacentric and submetacentric chromosomes as well as a large amount of constitutive heterochromatin, especially along the short arm of submetacentric chromosomes. In the present study, different chromosome banding methods were used to characterize the heterogeneous nature of the large heterochromatic segments and to propose an organizational model for this segment that occupies the entire short arm of most two-armed chromosomes. Furthermore, chromosome heteromorphism related to the short arm of...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200009

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)
Provedor de dados: 74
Autores: Luvesuto,Eloize; Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The culture of the marine shrimp Litopenaeus vannamei has recently boosted the Brazilian shrimp industry. However, it is well known that selection methods based solely on phenotypic characteristics, a reduced number of breeders and the practice of inbreeding may promote a significant raise in the genetic similarity of the captive populations, leading to greater disease susceptibility and impairing both the growth and final size of the shrimps. We used four microsatellite loci to investigate genetic variation in three generations (F5, F6 and F7) of a closed and reared L. vannamei lineage. Although an accentuated heterozygosis deficit was detected, we also observed that the captive propagation of this lineage did not lead to a significant loss of genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broodstocks; Inbreeding; Penaeids; Selection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600021

Genetic distance between broodstocks of the marine shrimp Litopenaeus vannamei (Decapoda, Penaeidae) by mtDNA analyses
Provedor de dados: 74
Autores: Francisco,Ana Karina de; Galetti Junior,Pedro Manoel.
In the constantly growing Brazilian shrimp industry the evaluation of genetic relationships between broodstocks is an useful tool for shrimp culture management programs. We established the genetic relationships between five broodstocks of the white marine shrimp Litopenaeus vannamei (Penaeidae) based on the sequencing of the mtDNA 16S rRNA and cytochrome oxidase I (COI) regions. Although no divergence was found between the broodstocks for the highly conservative 16S rRNA gene, we did find an 8.2% distance between L. vannamei and the Farfantepenaeus subtilis. Analyses of the COI region showed genetic distances of only 0.2 to 1% between the broodstocks, which contrasted with the 10.9% mean distance found between L. vannamei and F. subtilis. The small genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shrimp; Litopenaeus vannamei; Genetic variation; MtDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200014

New cases of supernumerary chromosomes in characiform fishes
Provedor de dados: 74
Autores: Venere,Paulo Cesar; Miyazawa,Carlos Suetoshi; Galetti Junior,Pedro Manoel.
Supernumerary chromosomes were described for five species of Neotropical characiform fishes. These extra chromosomes were small, acrocentric and fully heterochromatic in Leporinus friderici from two different localities as well as in Leporinus sp., but metacentric and fully heterochromatic in Cyphocharax modesta and Prochilodus nigricans. In Characidium cf. zebra, this element was small, acrocentric and euchromatic. GC-rich DNA blocks were observed in the supernumerary chromosome of Leporinus sp. using chromomycin A3. The widespread occurrence of these extra chromosomal elements suggests their independent origins.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300010

Karyotype description of two Neotropical Psittacidae species: the endangered Hyacinth Macaw, Anodorhynchus hyacinthinus, and the Hawk-headed Parrot, Deroptyus accipitrinus (Psittaciformes: Aves), and its significance for conservation plans
Provedor de dados: 74
Autores: Lunardi,Vitor de Oliveira; Francisco,Mercival Roberto; Rocha,Guaracy Tadeu; Goldschmidt,Beatriz; Galetti Junior,Pedro Manoel.
Neotropical parrots are among the most threatened groups of birds in the world, and many species are facing extinction in a near future. At the same time, the taxonomic position of many species remains unclear. Karyotype analysis has been used to elucidate the phylogenetic status of many bird groups, also providing important information for both in situ and ex situ conservation plans. The objective of the present study was to describe for the first time the karyotypes of the endangered Hyacinth Macaw, Anodorhynchus hyacinthinus, and of the Hawk-headed Parrot, Deroptyus accipitrinus. A diploid number of 2n = 70 and a karyotype similar to the main pattern previously found for the genera Ara, Cyanopsitta, Aratinga, Propyrrhura, Pionites, Pionopsitta,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian karyotype; Anodorhynchus; Deroptyus; Psittaciformes; Conservation biology; Cytotaxonomy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300011

Fluorescent amplified fragment length polymorphism (fAFLP) analyses and genetic diversity in Litopenaeus vannamei (Penaeidae)
Provedor de dados: 74
Autores: Gonçalves,Michelle Mantovani; Lemos,Manoel Victor Franco; Galetti Junior,Pedro Manoel; Freitas,Patrícia Domingues de; Furtado Neto,Manuel Antonio Andrade.
The Pacific white shrimp, Litopenaeus vannamei (Penaeidae), represents about 95% of all Brazilian shrimp production. The Brazilian L. vannamei foundation broodstock was made up of specimens collected from different American Pacific sites, but little information was collected on the genetic structure of the broodstock. We used the fluorescence amplified fragment length polymorphism (fAFLP) method to study the genetic diversity of L. vannamei broodstock lines 03CMF1 and 03CBF1 originally produced by breeder-shrimps imported mainly from Panama and Ecuador, although wild individuals from other localities may also have been used in producing these two lines. Our results showed a total of 93 polymorphic bands ranging from 50 to 500 bp, the mean Nei's genetic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: FAFLP; Genetic diversity; Litopenaeus vannamei; Penaeidae; Shrimp.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200016

PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
Provedor de dados: 74
Autores: Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; Genetic diversity; Shrimp; VNTR; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012

Chromosomal characterization of the bonytongue Arapaima gigas (Osteoglossiformes: Arapaimidae)
Provedor de dados: 83
Autores: Marques,Debora Karla; Venere,Paulo Cesar; Galetti Junior,Pedro Manoel.
The mitotic chromosomes of the pirarucu Arapaima gigas inhabiting the middle Araguaia River and collected in the municipality of Araguaiana (MT, Brazil) were studied. The chromosomes were analyzed through Giemsa staining, C-banding, Ag-NOR staining and in situ hybridization using an 18S rRNA gene probe. The karyotype had 2n=56 comprising 14 biarmed and 14 uniarmed chromosome pairs in both sexes. No cytologically distinguishable sex chromosome was identified. A single NOR-bearing chromosome pair was detected by Ag-NOR staining and confirmed by 18S rDNA- FISH. Faint constitutive heterochromatin was C-banded in the centromeric region of some chromosomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pirarucu; Karyotype; RDNA; Heterochromatin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252006000200007