Consulta: creatorFacets:"Ghosh,Jayadri Sekhar"
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Data/hora: 04/06/2024 00:23:46

Isolation of total RNA from hard bamboo tissue rich in polyphenols and polysaccharides for gene expression studies
Provedor de dados: 69
Autores: Rai,Vineeta; Ghosh,Jayadri Sekhar; Dey,Nrisingha.
RNA isolation from hard and woody internodal bamboo (Bambusa balcooa) tissue is very difficult due to the presence of secondary metabolites, polysaccharides, and polyphenolics. These compounds often co-precipitate with isolated RNA and hinder downstream applications. We have developed an efficient, cost effective and reproducible RNA isolation method from hard tissue of bamboo internode. This protocol includes an additional organic solvent refinement steps to remove endogenous phenolic compounds and acidic phenol (pH 4.2) to critically stabilize RNA in extraction buffer. In addition to these, two 2M Lithium chloride washing steps were introduced to eliminate DNA and polysaccharides contamination. The RNA isolated from the present protocol was found to be...
Tipo: Journal article
Palavras-chave: Acid phenol; Bambusa balcooa; Fiber specific genes; Internode; RNA extraction.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500022