Consulta: creatorFacets:"Gimpel, Carla"
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Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ovando, Fernanda; Gimpel, Carla; Cardenas, Constanza; Machado Cunha Da Silva, Jose Roberto; De Lorgeril, Julien; Gonzalez, Marcelo.
We have cloned and characterized for the first time an allograft inflammatory factor 1 (Sn-AIF-1) from the Antarctic sea urchin. We report the cloning of Sn-AIF-1 cDNA and the characterization of its expression in coelomocytes after a bacterial challenge. The cDNA Sn-AIF-1 has a size of 608 bp and encodes a polypeptide of 151 aa. The deduced amino acid sequence has a putative size of 17.430 Da, an isoelectric point of 4.92, and shows 2 elongation factor handlike motifs that normally bind calcium ions. BLAST analysis revealed close matches with other known AIF-1. The deduced amino acid sequence of Sn-AIF-1 showed high homology with AIF-1 in vertebrates such as fish, mice, and humans; and in the case of invertebrates, the major degree of identity (55%) was...
Tipo: Text
Palavras-chave: Antarctica; Sea urchin; Sterechinus neumayeri; Coelomocytes; Gene expression; AIF-1.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20955/18572.pdf