Consulta: creatorFacets:"Giongo,Adriana"
Registros recuperados: 8
Data/hora: 09/06/2024 02:57:57

Amplification of 16S rRNA gene sequences to differentiate two highly related bradyrhizobia species
Provedor de dados: 86
Autores: Giongo,Adriana; Ambrosini,Adriana; Freire,João Ruy Jardim; Zanettini,Maria Helena Bodanese; Passaglia,Luciane Maria Pereira.
A 16S rRNA gene PCR-based assay was developed aiming at a fast molecular diagnostic method to differentiate the two phylogenetically closely related species Bradyrhizobium japonicum and B. elkanii, isolated from soybean nodules, in order to identify those more competitive and comprising greater nitrogen fixation ability for use in the formulation of commercial inoculants. The assay used was able to discriminate ten reference strains belonging to these two Bradyrhizobium species, as well as to efficiently identify 37 strains isolated from fields cultivated with soybean.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Rhizobial diversity; Commercial inoculants.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900019

Isolation and characterization of two plant growth-promoting bacteria from the rhizoplane of a legume (Lupinus albescens) in sandy soil
Provedor de dados: 90
Autores: Giongo,Adriana; Beneduzi,Anelise; Ambrosini,Adriana; Vargas,Luciano Kayser; Stroschein,Marcos Roberto; Eltz,Flávio Luiz; Bodanese-Zanettini,Maria Helena; Passaglia,Luciane Maria Pereira.
Two bacterial strains that amplified part of the nifH gene, RP1p and RP2p, belonging to the genus Enterobacter and Serratia, were isolated from the rhizoplane of Lupinus albescens. These bacteria are Gram-negative, rod-shaped, motile, facultative anaerobic, and fast-growing; the colonies reach diameters of 3-4 mm within 24 h of incubation at 28 ºC. The bacteria were also able to grow at temperatures as high as 40 ºC, in the presence of high (2-3 % w/v) NaCl concentrations and pH 4 -10. Strain RP1p was able to utilize 10 of 14 C sources, while RP2p utilized nine. The isolates produced siderophores and indolic compounds, but none of them was able to solubilize phosphate. Inoculation of L. albescens with RP1p and RP2p strains resulted in a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indolic compounds; Siderophores; Inoculation experiment.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000200009

Rhizobial Inoculation, Alone or Coinoculated with Azospirillum brasilense, Promotes Growth of Wetland Rice
Provedor de dados: 90
Autores: Hahn,Leandro; Sá,Enilson Luiz Saccol de; Osório Filho,Benjamin Dias; Machado,Rafael Goulart; Damasceno,Raquel Garibaldi; Giongo,Adriana.
ABSTRACT Rhizobia and associative bacteria promote growth in rice plants (Oryza sativa L.) through a series of mechanisms, but most studies on inoculation have been performed based on inoculation with these bacteria in a separate or singular manner. The objective of this study was to assess the efficiency of single/isolated inoculation and inoculation combined with symbiotic rhizobia from forage legume and with Azospirillum brasilense on promoting growth and the root colonization process in wetland rice. Two rhizobia among four isolates from a greenhouse and a laboratory experiment were selected that efficiently promoted seed germination and rice plant growth in a sterilized substrate and in soil. The two most efficient isolates (UFRGS Vp16 and UFRGS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant growth-promoting rhizobacteria; Oryza sativa; Gfp tagging.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832016000100423

Occurrence of plant growth-promoting traits in clover-nodulating rhizobia strains isolated from different soils in Rio Grande do Sul state
Provedor de dados: 90
Autores: Vargas,Luciano Kayser; Lisboa,Bruno Brito; Schlindwein,Gilson; Granada,Camille Eichelberger; Giongo,Adriana; Beneduzi,Anelise; Passaglia,Luciane Maria Pereira.
In the last decades, the use of plant growth-promoting rhizobacteria has become an alternative to improve crop production. Rhizobium leguminosarum biovar trifolii is one of the most promising rhizobacteria and is even used with non-legume plants. This study investigated in vitro the occurrence of plant growth-promoting characteristics in several indigenous R. leguminosarum biovar trifolii isolated from soils in the State of Rio Grande do Sul, Brazil. Isolates were obtained at 11 locations and evaluated for indoleacetic acid and siderophore production and inorganic phosphate solubilization. Ten isolates were also molecularly characterized and tested for antagonism against a phytopathogenic fungus and for plant growth promotion of rice seedlings. Of a total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizobacteria; Siderophore; Indoleacetic acid; Phosphate solubilization; Antagonism; Rice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500016

Symbiotic efficiency and genetic characteristics of Bradyrhizobium sp. strain UFSM LA 1.3 isolated from Lupinus albescens (H. et Arn)
Provedor de dados: 63
Autores: Stroschein,Marcos Roberto Dobler; Eltz,Flávio Luiz Foletto; Antoniolli,Zaida Inês; Lupatini,Manoeli; Vargas,Luciano Kaiser; Giongo,Adriana; Pontelli,Mateus Padoin.
Legume species belonging to the genus Lupinus are annual herb plants. The majority of them are indigenous to the Americas. They are known for nitrogen-fixing symbioses with soil bacteria collectively called rhizobia. The aim of this study was to characterize a rhizobium strain isolated from Lupinus albescens using phenotypic, symbiotic and molecular approaches. Strain UFSM LA 1.3 was tested in vitro according to several parameters: colony size, color and growing rate; acid or alkaline reaction in yeast mannitol media supplemented with bromothymol blue; gum production. Molecular characterization was evaluated by PCR technique using primers BOX A1-R and sequence analysis of the 16S-23S rDNA intergenic region (ITS). ITS sequencing fragments showed genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lupin; Rhizobia; Genotypic approaches.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000600012

Characterization of plant growth-promoting bacteria inhabiting Vriesea gigantea Gaud. and Tillandsia aeranthos (Loiseleur) L.B. Smith (Bromeliaceae)
Provedor de dados: 49
Autores: Giongo,Adriana; Beneduzi,Anelise; Gano,Kelsey; Vargas,Luciano Kayser; Utz,Laura; Passaglia,Luciane Maria Pereira.
Microorganisms that live inside and around a plant can supply it with essential substances, such as phytohormones and essential nutrients. The present investigation aimed to isolate and characterize the phyllosphere, the endophytic, and the water tank bacteria associated with Vriesea gigantea and Tillandsia aeranthos. The bacteria were tested for siderophore and indole-3-acetic acid (IAA) production, phosphate solubilization, and presence of the nif H gene. Genetic diversity of the bacterial isolates was evaluated by rep-PCR. Sixty-eight bacterial strains were isolated from 3 different microhabitats of V. gigantea and from 2 microhabitats of T. aeranthos bromeliad plants. Gram-positive, spore-forming bacilli comprised most bacterial isolates. All isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliads; PGPB; Diazotrophic bacteria; Water tank; Siderophore; IAA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032013000300080

Characterization of variants of Bradyrhizobium elkanii and B. japonicum and symbiotic behaviour in soybeans
Provedor de dados: 65
Autores: Kober,Marcia de Vargas; Sá,Enilson Luiz Saccol de; Freire,João Ruy Jardim; Giongo,Adriana.
Variation in rhizobia strains isn’t a desirable fact based mainly on the possibility of unexpected results on legume inoculation. In this work, we studied the variability on phenotypic characteristics and genetic stability of rhizobia strains recommended for soybean inoculation. Variants with stable colony morphology were obtained from Bradyrhizobium japonicum strain SEMIA 5080 and from B. elkanii SEMIA 5019. Variants from SEMIA 587 obtained by another author were also used. The variants differed on colony characteristics, nodulation capacity, nitrogen fixation efficiency and competitive ability for nodule formation in two soybean varieties (Jacui 7 and IAS 5). Symbiotic behavior varied according to plant variety. Only the variants 5019 G and 5019 P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Variants isolation; Inoculant production; Isoenzymes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000500020

Promoção de crescimento de arroz por rizóbios em diferentes níveis de adubação nitrogenada
Provedor de dados: 65
Autores: Osorio Filho,Benjamin Dias; Binz,Andréia; Lima,Rafael Friedrich; Giongo,Adriana; Sá,Enilson Luiz Saccol de.
RESUMO: Embora os rizóbios não apresentem a capacidade de fixar nitrogênio em plantas de arroz, estes podem estimular o crescimento da parte aérea e da raiz, aumentando a absorção de nutrientes, como o nitrogênio, e a produtividade. Com o trabalho, avaliou-se o efeito de rizóbios em promover o crescimento de plantas de arroz em função de doses de nitrogênio (N). Efetuou-se um experimento com vasos contendo solo e plantas da cultivar IRGA 424. As plantas foram inoculadas com oito isolados de rizóbios e adubadas com doses equivalentes a 0, 40, 80 e 160kg N ha-1. Avaliou-se a matéria seca e a quantidade de N acumulada na parte aérea e o perfilhamento das plantas. O efeito do N no crescimento de arroz aumentou com a inoculação de rizóbios. A matéria seca da...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gramíneas; Não-leguminosas; Nitrogênio; Oryza sativa.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300478