Consulta: creatorFacets:"Golim,M.A."
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Characterization of mesenchymal stem cells derived from equine adipose tissue
Provedor de dados: 45
Autores: Carvalho,A.M.; Yamada,A.L.M.; Golim,M.A.; Álvarez,L.E.C.; Jorge,L.L.; Conceição,M.L.; Deffune,E.; Hussni,C.A.; Alves,A.L.G.
Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunophenotypic characterization; Differentiation; Equine; Adipose tissue; Mesenchymal stem cell.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400001