Consulta: creatorFacets:"Gomes de Andrade Lima,Maria Alice"
Registros recuperados: 2
Data/hora: 01/06/2024 02:30:56

The influence of centrifugation on Zymomonas mobilis aggregation
Provedor de dados: Electronic Journal of Biotechnology
Autores: Perez Fernandez Palha,María de los Angeles; Edison Lopes,Carlos; Gomes de Andrade Lima,Maria Alice; Pereira Junior,Nei.
Flocculent Zymomonas mobilis breaks down in smaller flocs and individual cells when centrifuged. The main consequence of it is an increase in the dispersion of the sample, suggesting that the influence of the centrifugal force on the aggregation of cells is worth to study. The experiments showed that the degree of dispersion varied between 30% and 100% when the centrifugal acceleration changed from 25 g to 2500 g. Observation under the electronic microscope showed that a slimy material covered the cells recovered by gentle gravitational settling and, that the centrifuged cells presented a bare cell wall.
Tipo: Journal article
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300011

Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides
Provedor de dados: Electronic Journal of Biotechnology
Autores: Santana de Almeida,Ana Cláudia; Costa de Araújo,Luciares; Mendes Costa,Andressa; Moraes de Abreu,César Augusto; Gomes de Andrade Lima,Maria Alice; Perez Fernandez Palha,Maria de Los Angeles.
The enzyme known as invertase (E.C. 3.2.1.26 - &amp;#946;-D-fructofuranosidase) catalyzes the sucrose hydrolysis producing an equimolar mixture of glucose and fructose named inverted sugar. The fungus Cladosporium cladosporioides has invertase as its constituent. Hence, its use as a natural immobilized support for the invertase produces interesting results for the enzyme. The present work has the objective of determining the optimum operational conditions of auto-immobilized invertase, as well as its kinetic parameters (K M and Vmax). A complete 2³ factorial planning was done for the evaluation of such parameters. Temperature, pH and agitation level were the studied variables. The hydrolysis percentage was the monitored result. Batch tests in optimum...
Tipo: Journal article
Palavras-chave: Cladosporium cladosporioides; Factorial planning; Invertase.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100009