Consulta: creatorFacets:"Gontijo,Cristiano Machado"
Registros recuperados: 3
Data/hora: 13/06/2024 04:55:19

Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates
Provedor de dados: 52
Autores: Oliveira,Flávia Márcia; Ruiz-de-Souza,Valéria; Campana-Pereira,Maria Aparecida; Gontijo,Cristiano Machado.
One of the mechanisms by which adjuvants are believed to promote T-cell activation and prevent induction of oral tolerance is by up-regulating the expression of co-stimulatory molecules on antigen presenting cells. Mice treated orally with palmitoyl-ovalbumin conjugates become immunized, while those treated with native ovalbumin (Ova) become tolerant. Cells from the peritoneal cavity of B6D2F1 mice were cultured in the presence of 0.01, or 0.1 mg/100ml of either Ova, or palmitoyl-Ova and tested for the presence of cell markers. PE-conjugated anti-mouse CD80, CD86, and CD11b antibodies as well as biotin-PE were used to stain the antigen-activated peritoneal cells. A significant increase in the expression of CD86 and CD80 was observed following in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovalbumin; Palmitic Acid; Cellular Immunity; CD86; CD80; CD11b.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600011

Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes
Provedor de dados: 52
Autores: Alves,Andrea Catão; Gontijo,Cristiano Machado; Oliveira,Mônica Cristina; Diniz,Simone Odília Fernandes; Oliveira,Flávia Márcia; Cardoso,Valbert Nascimento; Ramaldes,Gilson Andrade.
The oral administration of proteic antigens, like ovalbumin, may result in the induction of oral tolerance or immunization. The aim of this work was to label a protein antigen with 99mTechnetium, encapsulate it in liposomes and investigate its absorption and tissue distribution after oral administration in mice. Ovalbumin was labeled with 99mTechnetium and encapsulated in small unilamellar vesicles. 99mTc-OVA encapsulated or not in liposomes was administrated to mice that were sacrificed after different times. The radioactivity was measured in various organs of the animals. Differences concerning the biodistribution of 99mTc-OVA were noticed. The technique may represent alternatives for the induction of immunization or oral tolerance.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liposomes; Oral absorption; Mucosal delivery; Proteins; Nanotechnology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700035

Ethanol affects the absorption and tissue distribution of orally administered antigens in mice
Provedor de dados: 52
Autores: Oliveira,Flávia Márcia; Gusmão,Leandro José; Cardoso,Valbert do Nascimento; Ramaldes,Gilson Andrade; Ruiz-de-Souza,Valéria; Gontijo,Cristiano Machado.
The aim of this work was to evaluate the effects of ethanol on the adsorption and tissue distribution of orally administered antigens in mice. Results showed that ethanol reduced the level of anti-ovalbumin IgA antibodies in intestinal fluid for the mice treated orally with a palmitoyl-ovalbumin conjugate. Ethanol was administered intragastrically to mice at 5 g/kg body weight for 14 days (chronic treatment), or 10 g/kg body weight every 7th day up to 14 days (acute treatment). Thereafter, 99m technetium-labeled antigens were administered and lymphoid tissues were collected. Ethanol interfered with the transport of ovalbumin to the liver. Moreover, the transport of palmitoyl-ovalbumin to mesenteric lymph nodes was reduced 6 h after the antigen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Absorption; Biodistribution; Technetium; Immune response.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400008