Consulta: creatorFacets:"Gorlach-Lira,Krystyna"
Registros recuperados: 4
Data/hora: 13/06/2024 07:21:15

The abundance of some pathogenic bacteria in mangrove habitats of Paraiba do Norte estuary and crabmeat contamination of mangrove crab Ucides cordatus
Provedor de dados: 52
Autores: Grisi,Teresa Cristina Soares de Lima; Gorlach-Lira,Krystyna.
Study on mangrove water and sediment of Paraiba do Norte river (Northeastern Brazil) and crabmeat of Ucides cordatus was carried to investigate the relation between the abundance of bacteria in the mangrove habitat and in the crabmeat. Simultaneous collection of samples of water, sediment and crabs (U. cordatus) was done during the rainy and dry seasons at two areas of mangroves that differed in influence of tide and sewage discharge. The numbers of total heterotrophic bacteria in the mangrove water were between 0.04 and 6 log CFU 100mL-1, and in the sediment between 5 and 6 log CFU g-1. The location closer to waste discharge showed higher incidence of total heterotrophic bacteria, coliforms, Staphylococcus aureus and Salmonella spp. The counts of total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangrove ecosystem; Bacteria; Ucides cordatus; Crabmeat; Contamination.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100028

Response surface analysis on the effect of temperature and pH on Growth and proteolytic activity of thermophilic Bacillus sp
Provedor de dados: 52
Autores: Gorlach-Lira,Krystyna; Pedroza,Maria Lourdes Vieira; Burdziej-Pokojska,Aleksandra; Rozycki,Henryk; Dahm,Hanna.
Proteolytic activity and cell biomass of thermophilic Bacillus sp. strain were evaluated at various levels of initial pH and temperature by applying response surface methodology. The mineral medium containing yeast extract (0.01%) and starch (1%) was used throughout the experiment. The results of statistical analysis revealed the polynomial model with high coefficient of determination (R² = 0.8) for the biomass and total proteolytic activity of the strain studied. This model showed a satisfactory adjustment of the statistic model with the experimental data. The p values showed that the temperature and pH had significant effect on biomass and proteolytic activity (P&lt;0.05) of strain tested. The highest proteolytic activity (2.333 U/ml/h) of the Bacillus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus; Proteolytic activity; Response surface methodology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500009

Population dynamics and extracellular enzymes activity of mesophilic and thermophilic bacteria isolated from semi-arid soil of Northeastern Brazil
Provedor de dados: 58
Autores: Gorlach-Lira,Krystyna; Coutinho,Henrique D.M.
The dynamics of mesophilic and thermophilic bacterial population was studied in the bulk soil and rhizosphere of the grass Aristida adscensionis L. in a caatinga of the semi-arid Brazilian Northeast. Mesophilic heterotrophic bacteria, sporeforming bacteria and actinomycetes were significantly more abundant than thermophiles, and their counts were 10(6) - 10(7) CFU g-1 dry soil, showing weak fluctuations over the one-year study period. Thermophiles were below 10(6) CFU g-1 dry soil and, in general, demonstrated stronger fluctuations over the studied period. Among them, thermophilic actinomycetes showed the greatest decline during the dry season. No significant differences in microbial number were observed between bulk and rhizosphere soil. Mesophilic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesophilic bacteria; Thermophilic bacteria; Actinomycetes; Extracellular enzymes; Semi-arid; Caatinga.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100028

Action of nisin and high pH on growth of Staphylococcus aureus and Salmonella sp. in pure culture and in the meat of land crab (Ucides cordatus)
Provedor de dados: 58
Autores: Grisi,Teresa Cristina S. de Lima; Gorlach-Lira,Krystyna.
The aim of this study was to evaluate the potential of nisin and high pH to inhibit the growth of Staphylococcus aureus and Salmonella sp. in broth culture and when inoculated into meat of land crab. In pure cultures, the growth of S. aureus was bly inhibited by nisin and the growth of Salmonella sp. was inhibited by nisin-EDTA (20 mM). The inhibition of S. aureus lasted for eight hours and Salmonella sp. growth was inhibited throughout the experiment (24 h). The high pH (pH 10.0 and 11.0 with NaHCO3-NaOH buffer) was very effective for in vitro inhibition of S. aureus and Salmonella sp. Nisin and high pH, when applied to the contaminated meat, did not yield the same effect. Nisin was not effective in preventing growth of both pathogens in the crab meat,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crab meat; Nisin; High pH; Staphylococcus aureus; Salmonella sp.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200010