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A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
Provedor de dados: Electronic Journal of Biotechnology
Autores: Revelo Romo,Dolly Margot; Grosso,María Victoria; Moreno Solano,Nubia Carmenza; Montoya Castaño,Dolly.
A molecular approach was used for selecting polyhydroxyalcanoate (PHA)-accumulating potential Gram-negative bacteria from different genera by colony polymerase chain reaction (PCR). Three degenerate primers were designed for amplifying a fragment from PHA synthase gene (phaC) (Class I), phaC1 and phaC2 (Class II) genes for detecting PHA-producing bacteria. Thirty-four out of 55 bacterial strains from the old collection selected using Sudan black B staining were phaC+. PCR was used for directly selecting 35 new collection bacterial strains; these strains were phaC+ and their ability to produce PHA was confirmed by Sudan black B staining. Four specific primers were designed on genes of Class II PHA biosynthesis operon. These primers were used for evaluating...
Tipo: Journal article
Palavras-chave: 16S rDNA; Colony PCR; Degenerate primers; PHA synthase; Polyhydroxyalcanoate; Seminested PCR.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300002