Consulta: creatorFacets:"Grousset, Evelyse"
Registros recuperados: 3
Data/hora: 10/06/2024 19:03:55

Differential distribution of V-type H+-ATPase and Na+/K+-ATPase in the branchial chamber of the palaemonid shrimp Macrobrachium amazonicum
Provedor de dados: 5
Autores: Boudour-boucheker, Nesrine; Boulo, Viviane; Charmantier-daures, Mireille; Grousset, Evelyse; Anger, Klaus; Charmantier, Guy; Lorin-nebel, Catherine.
V-H+-ATPase and Na+/K+-ATPase were localized in the gills and branchiostegites of M. amazonicum and the effects of salinity on the branchial chamber ultrastructure and on the localization of transporters were investigated. Gills present septal and pillar cells. In freshwater (FW), the apical surface of pillar cells is amplified by extensive evaginations associated with mitochondria. V-H+-ATPase immunofluorescence was localized in the membranes of the apical evaginations and in clustered subapical areas of pillar cells, suggesting labeling of intracellular vesicle membranes. Na+/K+-ATPase labeling was restricted to the septal cells. No difference in immunostaining was recorded for both proteins according to salinity (FW vs. 25 PSU). In the branchiostegite,...
Tipo: Text
Palavras-chave: Osmoregulation; Na+/K+-ATPase; V-type H+-ATPase; Gills; Branchiostegite.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00202/31352/37901.pdf

Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (decapoda, penaeidae): Deciphering the role of the Na+/K+-ATPase
Provedor de dados: 5
Autores: Pham, Dominique; Charmantier, Guy; Boulo, Viviane; Wabete, Nelly; Ansquer, Dominique; Dauga, Clement; Grousset, Evelyse; Labreuche, Yannick; Charmantier-daures, Mireille.
The role of the main ion transporting enzyme Na +/K + − ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the...
Tipo: Text
Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Na+/K+-ATPase; RNA interference.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00311/42176/41477.pdf

Effects of fasting and re-alimentation on gill and intestinal morphology and indicators of osmoregulatory capacity in genetically selected sea bass (Dicentrarchus labrax) populations with contrasting tolerance to fasting
Provedor de dados: 5
Autores: Alix, Maud; Blondeau-bidet, Eva; Grousset, Evelyse; Shiranghi, Ainaz; Vergnet, Alain; Guinand, Bruno; Chatain, Beatrice; Boulo, Viviane; Lignot, Jehan-herve.
Fasting and refeeding occur naturally in predators but this is largely ignored when dealing with farmed fish. Therefore, the effects of 3-week fasting and re-alimentation (2.5% of the individual body mass) were investigated using two genetically selected populations (F2 generation) of 250 g juvenile sea bass (Dicentrarchus labrax L.). Blood osmolarity, gill and intestinal morphology and expression of the sodium pump (Na+, K+-ATPase, NKA) were studied on two phenotypes showing different degrees of body mass loss during food deprivation: one group losing body mass rapidly during fasting (F +) and the other one limiting body mass loss during the same period (F-).  Blood osmotic pressure significantly decreases due to re-alimentation in both groups, but this...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00354/46521/46285.pdf