Consulta: creatorFacets:"Guedes Pinto, Henrique"
Registros recuperados: 2
Data/hora: 02/06/2024 13:28:58

Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bouilly, Karine; Leitao, Alexandra; Chaves, Raquel; Guedes Pinto, Henrique; Boudry, Pierre; Lapegue, Sylvie.
Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pacific oyster; Restriction enzyme digestion chromosome banding; Atrazine; Aneuploidy; Crassostrea gigas; Huître creuse; Marquage chromosomique par digestion d'enzymes de restriction; Atrazine; Aneuploïdie.
Ano: 2005
URL: https://archimer.ifremer.fr/doc/2005/publication-654.pdf

Cytogenetic study of Ostrea conchaphila (Mollusca : Bivalvia) and comparative karyological analysis within Ostreinae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Leitao, Alexandra; Chaves, Raquel; Santos, Sara; Boudry, Pierre; Guedes Pinto, Henrique; Thiriot Quievreux, Catherine.
Chromosome preparations of the Olympia oyster Ostrea conchaphila Carpenter were studied using conventional Giemsa, silver staining, and C-banding techniques. The karyotype consists of six metacentric (1, 2, 4, 6, 8, and 10) and four submetacentric (3, 5, 7, 9) chromosome pairs. The silver-stained nucleolus organizer regions (Ag-NORs) were terminally located on the short arms of the submetacentric pair 5 (56% of cases) and on the long arms of submetacentric pair 7 (6% of cases). Constitutive heterochromatin was observed as telomeric C-bands on the short arm of the NOR-bearing chromosome pair 5 and as centromeric blocks of several chromosome pairs. Comparative analysis of patterns of karyotype, Ag-NORs, and C-bands of this species and of five other flat...
Tipo: Text
Palavras-chave: Ostreinae; Cytotaxonomy; Cytogenetics; Ostrea conchaphila.
Ano: 2002
URL: https://archimer.ifremer.fr/doc/2002/publication-2792.pdf