Consulta: creatorFacets:"Guimarães,Rafael Lima"
Registros recuperados: 3
Data/hora: 09/06/2024 04:45:18

Chlamydia infection in patients with and without cervical intra-epithelial lesions tested by real-time PCR vs. direct immunofluorescence
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Oliveira,Micheline de Lucena; Amorim,Melania Maria Ramos de; Souza,Paulo Roberto Eleutério de; Albuquerque,Lúcia Cristina Bezerra de; Brandão,Lucas André Cavalcanti; Guimarães,Rafael Lima.
This study compares the detection rates of Chlamydia trachomatis by two techniques, direct immunofluorescence (IMF) and real time polymerase chain reaction (PCR), in patients with and without intra-epithelial cervical lesions (SIL) in Recife. We conducted a transversal study involving 35 women with SIL and 35 without SIL attended at Ambulatório Especializado da Mulher, Recife, Brazil. They were tested for Chlamydia trachomatis using two techniques, direct IMF or real time PCR. The rates of Chlamydia trachomatis detection were compared and the association with intra-epithelial cervical lesions was determined using the chi-square test at a 5% level of significance. Concordance between the tests was evaluated using kappa. The global prevalence of Chlamydia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamydia trachomatis; Chlamydia infections; Fluorescent antibody technique direct; Polymerase chain reaction.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400014

Principles and applications of polymerase chain reaction in medical diagnostic fields: a review
Provedor de dados: Brazilian Journal of Microbiology
Autores: Valones,Marcela Agne Alves; Guimarães,Rafael Lima; Brandão,Lucas André Cavalcanti; Souza,Paulo Roberto Eleutério de; Carvalho,Alessandra de Albuquerque Tavares; Crovela,Sergio.
Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conventional PCR; Real-Time PCR; Molecular Biology; Methods of molecular detection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100001

DEFB1 gene polymorphisms and tuberculosis in a Northeastern Brazilian population
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,Ronaldo Celerino da; Cruz,Heidi Lacerda Alves da; Brandão,Lucas André Cavalcanti; Guimarães,Rafael Lima; Montenegro,Lilian Maria Lapa; Schindler,Haiana Charifker; Segat,Ludovica; Crovella,Sergio.
Abstract β-Defensin-1, an antimicrobial peptide encoded by the DEFB1 gene, is known to play an important role in lung mucosal immunity. In our association study we analyzed three DEFB1 functional polymorphisms -52G&gt;A (rs1799946), -44C&gt;G (rs1800972) and -20G&gt;A (rs11362) in 92 tuberculosis patients and 286 healthy controls, both from Northeast Brazil: no association was found between the studied DEFB1 polymorphisms and the disease. However we cannot exclude that this lack of association could be due to the low number of subjects analyzed, as suggested by the low statistical power achieved for the three analyzed SNPs (values between 0.16 and 0.50).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Innate immunity; DEFB1; Genotyping; Polymorphism.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200389