Consulta: creatorFacets:"Gutierrez,Vanessa Pacciari"
Registros recuperados: 1
Data/hora: 04/06/2024 02:36:26

Structural determinants of the hyperalgesic activity of myotoxic Lys49-phospholipase A2
Provedor de dados: 80
Autores: Zambelli,Vanessa Olzon; Chioato,Lucimara; Gutierrez,Vanessa Pacciari; Ward,Richard John; Cury,Yara.
Abstract  Background  Bothropstoxin-I (BthTx-I) is a Lys49-phospholipase A2 (Lys49-PLA2) from the venom of Bothrops jararacussu, which despite of the lack of catalytic activity induces myotoxicity, inflammation and pain. The C-terminal region of the Lys49-PLA2s is important for these effects; however, the amino acid residues that determine hyperalgesia and edema are unknown. The aim of this study was to characterize the structural determinants for the Lys49-PLA2-induced nociception and inflammation.  Methods  Scanning alanine mutagenesis in the active-site and C-terminal regions of BthTx-I has been used to study the structural determinants of toxin activities. The R118A mutant was employed as this substitution decreases PLA2 myotoxicity. In addition, K115A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lys49-Phospholipase A2; Hyperalgesia; Site-directed mutagenesis; Myotoxic effect; Edema; Membrane damage.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100304