Consulta: creatorFacets:"Heinemann,Marcos Bryan"
Registros recuperados: 12
Data/hora: 03/06/2024 18:23:59

Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows
Provedor de dados: 86
Autores: Oliveira,Andre Penido; Heinemann,Marcos Bryan; Cortez,Adriana; Nascimento,Paula Maria Pires do; Leite,Romulo Cerqueira; Viana,João Henrique Moreira.
Abstract: The objective of this work was to investigate the presence of bovine herpesvirus type 1 (BoHV-1) in follicular fluid and in cumulus-oocyte complexes (COC) recovered from naturally infected cows but with no clinical signs of the disease. Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. The presence of the virus was investigated by PCR in COC and in follicular fluid. Viral DNA was not found in any of the samples. The obtained results suggest that serologically positive cows with no clinical signs of the disease offer negligible risk of transmitting BoHV-1 by COC or follicular fluid.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos indicus; Follicular fluid; Infectious bovine rhinotracheitis virus; Transmission risk; Viral DNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500676

Seroprevalence of antibodies against group A rotavirus in cattle from a pioneer frontier in Brazilian Amazon
Provedor de dados: 62
Autores: Brandão,Paulo Eduardo; Cortez,Adriana; Ferreira,Fernando; Ferreira Neto,José Soares; Gregori,Fábio; Heinemann,Marcos Bryan; Homem,Valéria Stacchini Ferreira; Rodriguez,Cesar Alejandro Rosales; Jerez,José Antonio.
Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruará municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seroepidemiologic studies; Antibodies; Rotavirus; Cattle; Amazonia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100003

Soroprevalência da anemia infecciosa eqüina, da arterite viral dos eqüinos e do aborto viral eqüino no município de Uruará, PA, Brasil
Provedor de dados: 62
Autores: Heinemann,Marcos Bryan; Cortez,Adriana; Souza,Maria do Carmo Custódio de; Gotti,Tatiana; Ferreira,Fernando; Homem,Valéria Stachini Ferreira; Ferreira Neto,José Soares; Soares,Rodrigo Martins; Sakamoto,Sidnei Mioshi; Cunha,Elenice Maria Sequetin; Richtzenhain,Leonardo José.
Os vírus da anemia infecciosa eqüina (VAIE), da arterite viral dos eqüinos (VAVE) e do aborto viral eqüino (Herpesvírus eqüino tipo 1, HVE-1) são agentes causadores de enfermidades nos eqüídeos que podem causar graves prejuízos econômicos. O objetivo do presente trabalho foi estimar a soroprevalência de anticorpos contra os vírus VAIE, VAVE e HVE-1, utilizando como unidades de análise os eqüídeos e as propriedades rurais do tipo familiar do município de Uruará, PA. Os anticorpos contra o VAIE foram pesquisados pela prova de imunodifusão em gel de ágar e os anticorpos contra o VAVE e o HVE-1 pela prova de soroneutralização. O tamanho da amostra foi estimado a partir de um total de 2069 propriedades, caracterizadas por agricultura familiar e ausência de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prevalência; Uruará (PA); Anemia infecciosa eqüina; Arterite animal.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100009

Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
Provedor de dados: 58
Autores: Sales,Mariana L.; Fonseca Júnior,Antônio Augusto; Orzil,Lívia; Alencar,Andrea Padilha; Silva,Marcio Roberto; Issa,Marina Azevedo; Soares Filho,Paulo Martins; Lage,Andrey Pereira; Heinemann,Marcos Bryan.
Mycobacterium tuberculosis is the major cause of tuberculosis in humans. This bacillus gained prominence with the occurrence of HIV, presenting itself as an important opportunistic infection associated with acquired immunodeficiency syndrome (AIDS). The current study aimed to develop a real-time PCR using Eva Green technology for molecular identification of M. tuberculosis isolates. The primers were designed to Rv1510 gene. Ninety nine samples of M. tuberculosis and sixty samples of M. bovis were tested and no sample of the bovine bacillus was detected by the qPCR. Statistical tests showed no difference between the qPCR and biochemical tests used to identify the Mycobacterium tuberculosis. The correlation between tests was perfect with Kappa index of 1.0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Diagnosis; Real time PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400029

Development and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples
Provedor de dados: 58
Autores: Miotto,Bruno Alonso; Hora,Aline Santana da; Taniwaki,Sueli Akemi; Brandão,Paulo Eduardo; Heinemann,Marcos Bryan; Hagiwara,Mitika Kuribayashi.
Abstract A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy dogs were tested for presence of leptospiral DNA using the TaqMan-based assay. The results were compared with those from a well-established conventional PCR targeting the 16S RNA encoding gene associated with nucleotide sequencing analysis. The overall agreement between the assays was 94.8% (confidence interval [CI] 95% 88-100%). The newly developed assay presented 91.6% (CI 95% 71.5-98.5%) relative sensitivity (22[+] lipl32 PCR/24[+] 16S RNA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Dog; Urine; QPCR; Lipl32.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300584

Etiologic diagnosis of bovine infectious abortion by PCR
Provedor de dados: 65
Autores: Silva,Teane Milagres Augusto da; Oliveira,Raquel Gonzaga de; Mol,Juliana Pinto da Silva; Xavier,Mariana Noyma; Paixão,Tatiane Alves da; Cortez,Adriana; Heinemann,Marcos Bryan; Richtzenhain,Leonardo José; Lage,Andrey Pereira; Santos,Renato de Lima.
Infectious abortion is a significant cause of reproductive failure and economic losses in cattle. The goal of this study was to detect nucleic acids of several infectious agents known to cause abortion including Arcanobacterium pyogenes, Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetus subsp. venerealis, Chlamydophila abortus, Leptospira sp., Listeria monocytogenes, Salmonella sp., Mycoplasma bovis, Mycoplasma bovigenitalium, Neospora caninum, and Tritrichomonas foetus. Tissue homogenates from 42 fetuses and paraffin-embedded tissues from 28 fetuses and 14 placentas/endometrium were included in this study. Brucella abortus was detected in 14.2% (12/84) of the samples. Salmonella sp. DNA was amplified from 2 fetuses, and there was one positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Abortion; Brucella abortus; Salmonella sp.; Neospora caninum; Listeria monocytogenes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900028

Virulence factors and phylotyping of Escherichia coli isolated from non-diarrheic and diarrheic water buffalo calves
Provedor de dados: 65
Autores: Coura,Fernanda Morcatti; Diniz,Soraia de Araújo; Silva,Marcos Xavier; Oliveira,Cairo Henrique Sousa de; Mussi,Jamili Maria Suhet; Oliveira,Camila Stefanie Fonseca de; Lage,Andrey Pereira; Heinemann,Marcos Bryan.
ABSTRACT:  This study aimed to determine the virulence factors, phylogenetic groups, and the relationships between pathovars and phylogenetic groups of E. coli strains isolated from feces of buffalo calves. A total of 217 E. coli strains were obtained from feces after culture and were screened by PCR for detection of virulence factors EAST-1, enterohemolysin, Saa, CNF2, F41, F5, STa, intimin, Stx1 and Stx2. One hundred and thirty-four isolates were positive for one or more virulence factors: eighty-four from diarrheic animals, and fifty from non-diarrheic calves. The pathovars of E. coli identified in diarrheic feces were ETEC (F5+) (2/84), NTEC (16/84), STEC (20/84), EPEC (3/84), EHEC (3/84), and EAEC (EAST-1+) (33/84). Pathovars identified in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffalo calves; Diarrhea; Escherichia coli; Pathovars; Phylogenetic group.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500451

Isolation and identification of Mycobacterium bovis in milk from cows in northeastern Brazil
Provedor de dados: 65
Autores: Ramos,Joelson Marcolino; Heinemann,Marcos Bryan; Ferreira Neto,José Soares; Filho,Antônio Francisco de Souza; Cárdenas,Nicolás Céspedes; Alves,Clebert José; Azevedo,Sérgio Santos de.
ABSTRACT: Milk samples from 16 cows that tested positive on the tuberculin test in the state of Paraíba, northeastern Brazil, were used for mycobacteria isolation and identification. Mycobacteria were isolated from five (31.25%) of the 16 milk samples; three samples were classified as M. bovis, and two as belonging to the Mycobacterium genus. This is probably the first study of isolation and identification of M. bovis in milk from cows in Northeastern Brazil, which suggests that humans are at risk of contamination by ingestion.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mycobacterium bovis; Isolation; Milk; PCR; Cattle.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202166

Equilíbrio eletrolítico e ácido-base em bovinos
Provedor de dados: 65
Autores: Freitas,Moisés Dias; Ferreira,Marina Guimarães; Ferreira,Paulo Marcos; Carvalho,Antônio Último; Lage,Andrey Pereira; Heinemann,Marcos Bryan; Facury Filho,Elias Jorge.
O conhecimento sobre a fisiologia e regulação dos eletrólitos e do equilíbrio ácido-base é necessário, pois a saúde do animal depende diretamente da composição normal dos fluídos nos diversos compartimentos corporais. Esta regulação está intimamente entrelaçada em um rigoroso e preciso controle, de modo que uma anormalidade em um destes compartimentos reflete na alteração dos demais. A avaliação do equilíbrio eletrolítico e ácido-base pode fornecer informações fundamentais para o diagnóstico, prognóstico e decisão terapêutica de diversas enfermidades nos bovinos, tais como diarreias neonatais, hemoparasitoses (anaplasmose e a babesiose), deslocamento de abomaso e processos de sépticos. As correções destas alterações com medidas terapêuticas adequadas são...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equilíbrio ácido-base; Equilíbrio eletrolítico; Bovinos.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001200028

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil
Provedor de dados: 65
Autores: Coura,Fernanda Morcatti; Diniz,Amanda Nadia; Oliveira Junior,Carlos Augusto; Lage,Andrey Pereira; Lobato,Francisco Carlos Faria; Heinemann,Marcos Bryan; Silva,Rodrigo Otávio Silveira.
ABSTRACT: This study identified the virulence genes, pathovars, and phylogenetic groups of Escherichia coli strains obtained from the feces of dogs with and without diarrhea. Virulence genes and phylogenetic group identification were studied using polymerase chain reaction. Thirty-seven E. coli isolates were positive for at least one virulence factor gene. Twenty-one (57.8%) of the positive isolates were isolated from diarrheal feces and sixteen (43.2%) were from the feces of non-diarrheic dogs. Enteropathogenic E. coli (EPEC) were the most frequently (62.2%) detected pathovar in dog feces and were mainly from phylogroup B1 and E. Necrotoxigenic E. coli were detected in 16.2% of the virulence-positive isolates and these contained the cytotoxic necrotizing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Escherichia coli; Pathovars; Phylogroups.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200452

Different methods of real-time PCR for detection of pseudorabies virus
Provedor de dados: 65
Autores: Nonaka,Carolina Kymie Vasques; Fonseca Junior,Antônio Augusto; Guedes,Estefânia Oliveira; D´Ambros,Régia Maria; Lima,Graciela Kunrath; Camargos,Marcelo Fernandes; Heinemann,Marcos Bryan.
ABSTRACT: Pseudorabies (PR) is a highly contagious viral disease of great animal health and economic importance in swine industry. The aim of this study was to evaluate different genomic regions, real-time PCR chemistries and equipment for the molecular diagnosis of PR. Eight primer pairs targeting four genes (gB, gC, gE, gD), three different qPCR chemistries (SybrGreen, hydrolysis probes and plexor) and two equipment (ABI7500, Rotorgene 3000) were evaluated. Oligonucleotides targeting gB using hydrolysis probes showed the best performance after evaluating efficiency (99%), the detection limit (10-1.5 TCID50 mL-1) and diagnostic sensitivity and; therefore, those primers were selected for performance verification factors such as repeatability,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Real time PCR; Pseudorabies; Diagnose.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300452

Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius in dogs with otitis externa
Provedor de dados: 65
Autores: Scherer,Carolina Boesel; Botoni,Larissa Silveira; Coura,Fernanda Morcatti; Silva,Rodrigo Otávio; Santos,Rafael Dantas dos; Heinemann,Marcos Bryan; Costa-Val,Adriane Pimenta.
ABSTRACT: Infections by Staphyloccocus pseudintermedius in the skin of dogs have been object of studies, since such microorganisms often present multiple resistance to antibiotics. This study aimed to identify and evaluate the antimicrobial susceptibility of Staphylococcus pseudintermedius (SP) strains isolated from dogs with otitis. Swabs from both ears of 52 dogs with otitis (n=104) were included. Bacteria were cultured using Muller-Hinton agar (supplemented with 5% equine blood and incubated at 37°C for 24 to 48 hours. All colonies underwent biochemical evaluation for identification of staphylococci. The identity of colonies as SP was confirmed by polymerase chain reaction. The antimicrobial susceptibility of SP strains was evaluated by disk diffusion....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; MRSP; Methicillin-resistant staphylococcus; Otitis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000400453