Consulta: creatorFacets:"Herculano,A.M."
Registros recuperados: 4
Data/hora: 16/06/2024 10:39:37

Therapeutic concentration of morphine reduces oxidative stress in glioma cell line
Provedor de dados: 56
Autores: Almeida,M.B.; Costa-Malaquias,A.; Nascimento,J.L.M.; Oliveira,K.R.; Herculano,A.M.; Crespo-López,M.E.
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphine; Glia; Oxidative stress; Hydrogen peroxide; Lipid peroxidation; Opiod.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500398

Local treatment with ascorbic acid accelerates recovery of post-sutured Achilles tendon in male Wistar rats
Provedor de dados: 56
Autores: Souza,M.; Moraes,S.A.S.; de Paula,D.R.; Maciel,A.A.; Batista,E.J.O.; Silva,D.G.F.; Bahia,C.P.; Oliveira,K.R.H.M.; Herculano,A.M.
Tendon rupture is a very frequent accident involving average people and high-performance athletes. Clinical studies describe tendon recovery as a painful and slow process involving different biochemical and histological events. Ascorbic acid (AA) is a potent antioxidant as well as an important cofactor for collagen synthesis. In the current study, we evaluated if local treatment with AA is able to promote tendon repair in tenotomized rats. Animals were submitted to Achilles tendon rupture followed by surgical suture. Control and AA groups received in loco injection of saline solution (0.9% NaCl) and 30 mM AA, respectively. Histological and functional recovery of Achilles tendon tissue was evaluated at 7, 14, and 21 days post-surgery. Hematoxylin/eosin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tendon recovery; Tenotomy; Ascorbic acid; Tendon suture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900608

Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions
Provedor de dados: 56
Autores: Seabra,A.D.; Moraes,S.A.S.; Batista,E.J.O.; Garcia,T.B.; Souza,M.C.; Oliveira,K.R.M.; Herculano,A.M.
Muscular atrophy is a progressive degeneration characterized by muscular proteolysis, loss of mass and decrease in fiber area. Tendon rupture induces muscular atrophy due to an intrinsic functional connection. Local inhibition of nitric oxide synthase (NOS) by Nω-nitro-L-arginine methyl ester (L-NAME) accelerates tendon histological recovery and induces functional improvement. Here we evaluate the effects of such local nitrergic inhibition on the pattern of soleus muscle regeneration after tenotomy. Adult male Wistar rats (240 to 280 g) were divided into four experimental groups: control (n=4), tenotomized (n=6), vehicle (n=6), and L-NAME (n=6). Muscular atrophy was induced by calcaneal tendon rupture in rats. Changes in muscle wet weight and total protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Muscle regeneration; Atrophy; Tendon; Tenotomy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300601

Methylmercury intoxication activates nitric oxide synthase in chick retinal cell culture
Provedor de dados: 56
Autores: Herculano,A.M.; Crespo-López,M.E.; Lima,S.M.A.; Picanço-Diniz,D.L.W.; Nascimento,J.L.M. Do.
The visual system is a potential target for methylmercury (MeHg) intoxication. Nevertheless, there are few studies about the cellular mechanisms of toxicity induced by MeHg in retinal cells. Various reports have indicated a critical role for nitric oxide synthase (NOS) activation in modulating MeHg neurotoxicity in cerebellar and cortical regions. The aim of the present study is to describe the effects of MeHg on cell viability and NOS activation in chick retinal cell cultures. For this purpose, primary cultures were prepared from 7-day-old chick embryos: retinas were aseptically dissected and dissociated and cells were grown at 37ºC for 7-8 days. Cultures were exposed to MeHg (10 µM, 100 µM, and 1 mM) for 2, 4, and 6 h. Cell viability was measured by MTT...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methylmercury; Chick retinal cell culture; Nitric oxide synthase; Cell viability; Neurotoxicity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300013