Consulta: creatorFacets:"Hervouet, Veronique"
Registros recuperados: 5
Data/hora: 09/06/2024 18:23:55

The use of microsatellite markers for parentage analysis in the Pacific cupped oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Kotoulas, Georgios; Magoulas, Antonio; Hervouet, Veronique; Bonhomme, Francois; Gerard, Andre.
Highly polymorphic genetic markers can be useful tools for the analysis of parental contributions. The present paper reports 3 experiments using microsatellite markers to analyse parental contributions in controlled in vitro factorial crosses of the cupped oyster Crassostrea gigas. Parentage analysis was eased by the large polymorphism observed at the 3 loci studied. In a cross between 20 males and 20 females, a quarter of the progeny could be assigned to a given parent using a single microsatellite locus showing 28 alleles. In 3 crosses between 5 males and females, all the progeny could be assigned to a family by using 3 loci. In all cases, the combined genotyping at 2 loci allowed parentage to be detennined unambiguously. Despite the balanced gametic...
Tipo: Text
Palavras-chave: Crassostrea gigas; Microsatellites; Parental contributions; Crassostrea gigas; Microsatellites; Contributions parentales.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/acte-2739.pdf

Utilisation d'un marqueur microsatellite pour l'étude des contributions parentales chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Hervouet, Veronique.
Les marqueurs génétiques très polymorphes sont des outils particulièrement intéressants pour l'analyse de contributions parentales. Dans notre étude, nous avons utilisé un marqueur microsatellite pour analyser les contributions parentales dans deux croisements contrôlés chez l'huître creuse Crassostrea gigas. 5 mâles et 5 femelles, hétérozygotes à ce locus, et portant chacun des allèles différents (soit 20 allèles ségrégés au total) ont été croisés de deux manières différents: soit en mélangeant toutes les gamètes mâles d'une part, toutes les gamètes femelles d'autre part, et en réalisant un croisement entre ces deux mélanges; soit en fécondant séparément chacune des 5 femelles par chacun de 5 mâles, soit 25 croisement séparés. Pour le deuxième type de...
Tipo: Text
Palavras-chave: Contributions parentales; Compétition; Microsatellite; Crassostrea gigas.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00032/14372/11661.pdf

Mise en évidence expérimentale de la variance du succès reproducteur chez l'huître creuse Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Kotoulas, G.; Bonhomme, Francois.
Oysters, like many other marine species, are characterized by very high fertilities. Natural or hatchery populations, often present very low Ne/N ratios (Hedgecock, 1994). It can be explained by very high variances of their breeding success (Li and Hedgecock, 1998), the effective population size being estimated through the temporal variance of the allelic frequencies in natural populations (Hedgecock et al., 1992) or, more directly, through the estimated number of founders in hatchery populations (Launey, 1998). This document is a presentation of the results of a number of experiments, realised in the scope of the European program "GENEPHYS" (FAIR 95-421), and using microsatellite markers to analyse the parental contributions in the flat oyster Crassostrea...
Tipo: Text
Palavras-chave: Microsatellite; Succès reproducteur; Contributions parentales; Génétique; Crassostrea gigas; Huîtres.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3454.pdf

Utilisation des marqueurs microsatellites pour l'étude des contributions parentales chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Kotoulas, G.; Hervouet, Veronique; Cornette, Florence; Bonhomme, Francois; Gerard, Andre.
Numerous aquacultural species show very high fertilities. As a consequence, it is possible to produce, in hatcheries, a large amount of sibs from a small amount of parents. The sibs' genetic variability is, of course, directly linked to the number of parents involved, but it may be largely lowered by imbalanced parental contributions. Polymorphic genetic markers are very handy tools in the study of these phenomena in cultivated populations (Harris et al. 1991; Herbinger et al., 1996a; Garcia de Leon et al. 1998) or in natural populations (Ferguson et al, 1995; Kellog et al., 1995; Colbourne et al., 1996; Herbinger et al., 1996b). This document is a presentation of the results of a number of experiments, realised in the scope of the European program...
Tipo: Text
Palavras-chave: Microsatellites; Contributions parentales; Crassostrea gigas; Huîtres.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3452.pdf

Microsatellite markers as a tool to study reproductive success in the Pacific oyster, Crassostrea gigas (Thunberg), under controlled hatchery conditions
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois.
Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of  Crassostrea gigas,  the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each...
Tipo: Text
Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf