Consulta: creatorFacets:"Hess, Philipp"
Registros recuperados: 91
Data/hora: 03/06/2024 18:16:22

Effect of environmental and nutritional factors on growth and azaspiracid production of the dinoflagellate Azadinium spinosum
Provedor de dados: 5
Autores: Jauffrais, Thierry; Sechet, Veronique; Herrenknecht, Christine; Truquet, Philippe; Savar, Veronique; Tillmann, Urban; Hess, Philipp.
Azadinium spinosum, a small dinoflagellate isolated from the North Sea, is a producer of azaspiracids (AZAs), a group of biotoxins associated with human illness following ingestion of contaminated shellfish. Using batch and continuous cultures of A. spinosum, the present study investigated the effects of different environmental and nutritional factors (salinity, temperature, photon flux density, aeration, culture media, nitrogen sources, phosphate source, and N/P ratios) on growth, maximum cell concentration, and AZA cell quota.  Azadinium spinosum grew in a wide range of conditions; from 10 ̊C to 26 ̊C and salinities from 30 to 40, under irradiances ranging from 50 μmol m−2 s−1 to 250 μmol m−2 s−1, with or without aeration. Growth and maximum cell...
Tipo: Text
Palavras-chave: AZA; Chemostat; Dinophyceae; Nutrients; N/P ratio; Photobioreactor.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00149/26054/24411.pdf

Cytotoxicity, Fractionation and Dereplication of Extracts of the Dinoflagellate Vulcanodinium rugosum, a Producer of Pinnatoxin G
Provedor de dados: 5
Autores: Geiger, Marie; Desanglois, Gwenaelle; Hogeveen, Kevin; Fessard, Valerie; Lepretre, Thomas; Mondeguer, Florence; Guitton, Yann; Herve, Fabienne; Sechet, Veronique; Grovel, Olivier; Pouchus, Yves-francois; Hess, Philipp.
Pinnatoxin G (PnTX-G) is a marine toxin belonging to the class of cyclic imines and produced by the dinoflagellate Vulcanodinium rugosum. In spite of its strong toxicity to mice, leading to the classification of pinnatoxins into the class of “fast-acting toxins”, its hazard for human health has never been demonstrated. In this study, crude extracts of V. rugosum exhibited significant cytotoxicity against Neuro2A and KB cells. IC50 values of 0.38 µg mL−1 and 0.19 µg mL−1 were estimated on Neuro2A cells after only 24 h of incubation and on KB cells after 72 h of incubation, respectively. In the case of Caco-2 cells 48 h after exposure, the crude extract of V. rugosum induced cell cycle arrest accompanied by a dramatic increase in double strand DNA breaks,...
Tipo: Text
Palavras-chave: Dereplication; Cyclic imine; HRMS; Bioactivity; Pinnatoxins.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00156/26723/24802.pdf

Extended evaluation of polymeric and lipophilic sorbents for passive sampling of marine toxins
Provedor de dados: 5
Autores: Zendong, Zita; Herrenknecht, Christine; Abadie, Eric; Brissard, Charline; Tixier, Celine; Mondeguer, Florence; Sechet, Veronique; Amzil, Zouher; Hess, Philipp.
Marine biotoxins are algal metabolites that can accumulate in fish or shellfish and render these foodstuffs unfit for human consumption. These toxins, released into seawater during algal occurrences, can be monitored through passive sampling. Acetone, methanol and isopropanol were evaluated for their efficiency in extracting toxins from algal biomass. Isopropanol was chosen for further experiments thanks to a slightly higher recovery and no artifact formation. Comparison of Oasis HLB, Strata-X, BondElut C18 and HP-20 sorbent materials in SPE-mode led to the choice of Oasis HLB, HP-20 and Strata-X. These three sorbents were separately exposed as passive samplers for 24 h to seawater spiked with algal extracts containing known amounts of okadaic acid (OA),...
Tipo: Text
Palavras-chave: Passive sampling; Oasis HLB; Strata-X; LOPE; PDMS; SPATT.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00186/29756/28187.pdf

A mussel (Mytilus edulis) tissue certified reference material for the marine biotoxins azaspiracids
Provedor de dados: 5
Autores: Mccarron, Pearse; Giddings, Sabrina D.; Reeves, Kelley L.; Hess, Philipp; Quilliam, Michael A.
Azaspiracids (AZAs) are lipophilic biotoxins produced by marine algae that can contaminate shellfish and cause human illness. The European Union (EU) regulates the level of AZAs in shellfish destined for the commercial market, with liquid chromatography-mass spectrometry (LC-MS) being used as the official reference method for regulatory analysis. Certified reference materials (CRMs) are essential tools for the development, validation, and quality control of LC-MS methods. This paper describes the work that went into the planning, preparation, characterization, and certification of CRM-AZA-Mus, a tissue matrix CRM, which was prepared as a wet homogenate from mussels (Mytilus edulis) naturally contaminated with AZAs. The homogeneity and stability of...
Tipo: Text
Palavras-chave: Azaspiracids; Seafood safety; Matrix certified reference material; Liquid chromatography-mass spectrometry; Matrix effects.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00320/43138/42761.pdf

Field and mesocosm trials on passive sampling for the study of adsorption and desorption behaviour of lipophilic toxins with a focus on OA and DTX1
Provedor de dados: 5
Autores: Fux, E; Marcaillou-le Baut, Claire; Mondeguer, Florence; Bire, R; Hess, Philipp.
It has been demonstrated that polymeric resins can be used as receiving phase in passive samplers designed for the detection of lipophilic marine toxins at sea and was referred to as solid phase adsorption toxin tracking (SPATT). The present study describes the uptake and desorption behaviour of the lipophilic marine toxins okadaic acid (OA) and dinophysistoxin-1 (DTX1) from Prorocentrum lima cultures by five styrene-divinylbenzene based polymeric resins Sepabeads (R) SP850, Sepabeads (R) SP825L, Amberlite (R) XAD4, Dowex (R) Optipore (R) L-493 and Diaion (R) HP-20. All resins accumulated OA and DTX1 from the P. lima culture with differences in adsorption rate and equilibrium rate. Following statistical evaluation, HP-20, SP850 and SP825L demonstrated...
Tipo: Text
Palavras-chave: Polymeric resin; Dinophysistoxin 1; Okadaic acid; SPATT; Prorocentrum lima.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4739.pdf

Combined effects of temperature, irradiance and pH on   Teleaulax amphioxeia   (Cryptophyceae) physiology and feeding ratio for its predator   Mesodinium rubrum   (Ciliophora)
Provedor de dados: 5
Autores: Gaillard, Sylvain; Charrier, Aurelie; Malo, Florent; Carpentier, Liliane; Bougaran, Gael; Hégaret, Helene; Réveillon, Damien; Hess, Philipp; Séchet, Veronique.
The cryptophyte Teleaulax amphioxeia is a source of plastids for the ciliate Mesodinium rubrum and both organisms are members of the trophic chain of several species of Dinophysis. It is important to better understand the ecology of organisms at the first trophic levels before assessing the impact of principal factors of global change on Dinophysis spp. Therefore, combined effects of temperature, irradiance and pH on growth rate, photosynthetic activity and pigment content of a temperate strain of T. amphioxeia were studied using a full factorial design (central composite design 23*) in 17 individually controlled bioreactors. The derived model predicted an optimal growth rate of T. amphioxeia at a light intensity of 400 µmol photons · m‐2 · s‐1, more...
Tipo: Text
Palavras-chave: Dinophysis; Ecophysiology; Full factorial design; Global change; Mesodinium rubrum; Teleaulax amphioxeia.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00609/72155/70910.pdf

Dissolved azaspiracids are absorbed and metabolized by blue mussels (Mytilus edulis)
Provedor de dados: 5
Autores: Jauffrais, Thierry; Kilcoyne, Jane; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Miles, Christopher O.; Hess, Philipp.
The relationship between azaspiracid shellfish poisoning and a small dinoflagellate, Azadinium spinosum, has been shown recently. The organism produces AZA1 and -2, while AZA3 and other analogues are metabolic products formed in shellfish. We evaluated whether mussels were capable of accumulating dissolved AZA1 and -2, and compared the toxin profiles of these mussels at 24 h with profiles of those exposed to live or lysed A. spinosum. We also assessed the possibility of preparative production of AZA metabolites by exposing mussels to semi-purified AZA1. We exposed mussels to similar concentration of AZAs: dissolved AZA1+2 (crude extract) at 7.5 and 0.75 µg L-1, dissolved AZA1+2 (7.5 µg L-1) in combination with Isochrysis affinis galbana, and lysed and live...
Tipo: Text
Palavras-chave: Dissolved marine biotoxins; AZA; Tissue distribution; Bivalve molluscs; LC-MS/MS; Azaspiracid.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00136/24757/22867.pdf

Effect of seawater salinity on pore-size distribution on a poly(styrene)-based HP20 resin and its adsorption of diarrhetic shellfish toxins
Provedor de dados: 5
Autores: Fan, Lin; Sun, Geng; Qiu, Jiangbing; Ma, Qimin; Hess, Philipp; Li, Aifeng.
In the present study, okadaic acid (OA) and dinophysistoxin-1 (DTX1) were spiked into artificial seawater at low, medium and high estuarine salinities (9‰, 13.5‰ and 27‰). Passive samplers (HP20 resin) used for solid phase adsorption toxin tracking (SPATT) technology were exposed in these seawaters for12-h periods. Adsorption curves well fitted a pseudo-secondary kinetics model. The highest initial sorption rates of both toxins occurred in the seawater of medium salinity, followed by seawater of low and high estuarine salinity. Pore volumes of micropores (&lt; 2 nm) and small mesopores (2 nm &lt; diameter &lt; 10 nm) of HP20 resin decreased after adsorption of toxins in seawater at high and low salinity but not in seawater at medium salinity, which...
Tipo: Text
Palavras-chave: Diarrhetic shellfish toxins (DST); Solid phase adsorption toxin tracking (SPATT); HP20 resin; Salinity; Pore-size distribution; Pseudo-secondary kinetics equation.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00226/33766/32192.pdf

Growth and toxin production of Azadinium spinosum in batch and continuous culture
Provedor de dados: 5
Autores: Jauffrais, Thierry; Sechet, Veronique; Herrenknecht, Christine; Tillmann, Urban; Krock, Bernd; Amzil, Zouher; Hess, Philipp.
Azaspiracids are lipophilic marine biotoxins causing gastrointestinal symptoms similar to DSP toxins. Since 1995, azaspiracids have been encountered in Europe, Africa and more recently in North and South America and Japan. The biological primary producer remained undiscovered during many years and has now been identified as Azadinium spinosum. The organism was grown using K modified medium, at 18°C with a PFD of 200 μmol.m-2.s-1 and a photoperiod of 16L/8D. Batch cultures were carried out using 75mL and 10L flasks, while continuous cultures were produced in 100L chemostats. Cells were recovered using centrifugation or filtration. Different extraction solvents and procedures as well as evaporation modes were evaluated for yield. Quantitation was carried out...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00152/26348/24431.pdf

Algal toxin profiles in Nigerian coastal waters (Gulf of Guinea) using passive sampling and liquid chromatography coupled to mass spectrometry
Provedor de dados: 5
Autores: Zendong, Suzie Zita; Kadiri, Medina; Herrenknecht, Christine; Nezan, Elisabeth; Mazzeo, Antonia; Hess, Philipp.
Algal toxins may accumulate in fish and shellfish and thus cause poisoning in consumers of seafood. Such toxins and the algae producing them are regularly surveyed in many countries, including Europe, North America, Japan and others. However, very little is known regards the occurrence of such algae and their toxins in most African countries. This paper reports on a survey of phytoplankton and algal toxins in Nigerian coastal waters.  Seawater samples were obtained from four sites for phytoplankton identification, on three occasions between the middle of October 2014 and the end of February 2015 (Bar Beach and Lekki in Lagos State, Port Harcourt in Rivers State and Uyo in Akwa Ibom State). The phytoplankton community was generally dominated by diatoms and...
Tipo: Text
Palavras-chave: Dinoflagellates; Dinophysis; Phycotoxins; Untargeted analysis; Phytoplankton.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00314/42521/41893.pdf

Ostreopsis cf. ovata Bloom in Currais, Brazil: Phylogeny, Toxin Profile and Contamination of Mussels and Marine Plastic Litter
Provedor de dados: 5
Autores: Tibiriçá, Carlos Eduardo J. A.; Leite, Isabel P.; Batista, Talita V. V.; Fernandes, Luciano F.; Chomérat, Nicolas; Herve, Fabienne; Hess, Philipp; Mafra, Luiz.
Ostreopsis cf. ovata is a toxic marine benthic dinoflagellate responsible for harmful blooms affecting ecosystem and human health, mostly in the Mediterranean Sea. In this study we report the occurrence of a summer O. cf. ovata bloom in Currais, a coastal archipelago located on the subtropical Brazilian coast (~25° S). This bloom was very similar to Mediterranean episodes in many aspects: (a) field-sampled and cultivated O. cf. ovata cells aligned phylogenetically (ITS and LSU regions) along with Mediterranean strains; (b) the bloom occurred at increasing temperature and irradiance, and decreasing wind speed; (c) cell densities reached up to 8.0 × 104 cell cm−2 on fiberglass screen and 5.6 × 105 cell g−1 fresh weight on seaweeds; (d) and toxin profiles...
Tipo: Text
Palavras-chave: Harmful algal bloom; Benthic microalgae; Toxic dinoflagellates; Ovatoxin; Toxin transfer; Seafood safety; Marine pollution; Plastic litter; Biofilm formation.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00509/62037/66177.pdf

Effect of Dilution Rate on Azadinium spinosum and Azaspiracid (AZA) Production in Pilot Scale Photobioreactors for the Harvest of AZA1 and -2
Provedor de dados: 5
Autores: Jauffrais, Thierry; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Herrenknecht, Christine; Hess, Philipp.
Azadinium spinosum, a small dinoflagellate has recently been discovered and identified as the primary producer of azaspiracid-1 (AZA) and -2. Since AZA poisoning has been reported following consumption of contaminated shellfish it is important to have these toxins available for toxicological studies, and a sustainable production of AZAs as calibrants in monitoring programs without having to rely on natural events.  In order to address this concern, continuous pilot scale cultures were carried out to evaluate the feasibility of AZA production from A. spinosum. Algae were cultured using two 100 L chemostats in series (R1 and R2), with agitation and pH control. Four different dilution rates were tested (0.15, 0.2, 0.25 and 0.3 day−1) to evaluate chemostat...
Tipo: Text
Palavras-chave: Solid phase adsorption; Chemostat; Photobioreactor; Microalgae; LC-MS/MS; Tangential flow filtration.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00152/26349/24437.pdf

Isolation, Structure Elucidation, Relative LC-MS Response, and in Vitro Toxicity of Azaspiracids from the Dinoflagellate Azadinium spinosum
Provedor de dados: 5
Autores: Kilcoyne, Jane; Nulty, Ciara; Jauffrais, Thierry; Mccarron, Pearse; Herve, Fabienne; Foley, Barry; Rise, Frode; Crain, Sheila; Wilkins, Alistair L.; Twiner, Michael J.; Hess, Philipp; Miles, Christopher O.
We identified three new azaspiracids (AZAs) with molecular weights of 715, 815, and 829 (AZA33 (3), AZA34 (4), and AZA35, respectively) in mussels, seawater, and Azadinium spinosum culture. Approximately 700 mu g of 3 and 250 mu g of 4 were isolated from a bulk culture of A. spinosum, and their structures determined by MS and NMR spectroscopy. These compounds differ significantly at the carboxyl end of the molecule from known AZA analogues and therefore provide valuable information on structure-activity relationships. Initial toxicological assessment was performed using an in vitro model system based on Jurkat T lymphocyte cytotoxicity, and the potencies of 3 and 4 were found to be 0.22- and 5.5-fold that of AZA1 (1), respectively. Thus, major changes in...
Tipo: Text
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00245/35606/34414.pdf

World Health Organization and international guidelines for toxin control, harmful algal bloom management, and response planning
Provedor de dados: 5
Autores: Soltani, Alex; Hess, Philipp; Dixon, Mike B; Boerlage, Siobhan F.e.; Anderson, Donald M; Newcombe, Gayle; House, Jenny; Ho, Lionel; Baker, Peter; Burch, Michael.
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00407/51836/52437.pdf

COLNACOQ. Composés lipophiles naturels dans l’environnement des coquillages. Rapport final
Provedor de dados: 5
Autores: Hess, Philipp; Geiger, Marie; Brochard, Solène; Lepretre, Thomas; Fessard, Valerie; Antignac, Jean-philippe; Dupont, Jacques; Sechet, Veronique; Vanel, Faustine; Mondeguer, Florence; Herve, Fabienne; Leborgne, Sabrina; Deslanglois, Gwenaëlle; Marshall, Lindsey; Rounds, Lucy; Guitton, Yann; Amzil, Zouher; Grovel, Olivier; Ruiz, Nicolas; Pouchus, Francois.
Cette étude s’est inscrite dans un contexte d’évaluation de la salubrité des mollusques bivalves destinés à la consommation humaine. Ces organismes peuvent en effet accumuler des toxines, en particulier de microalgues, en concentrations importantes, pouvant induire des intoxications chez les personnes les consommant. Afin d’assurer la protection des consommateurs, les toxines réglementées sont recherchées dans les coquillages par techniques physico-chimiques. En parallèle, les toxines émergentes, potentiellement produites par d’autres organismes que les micro-algues telles que les micromycètes, peuvent être detectées par l’utilisation du test de toxicité aigüe sur souris. Cependant, ce bio-essai présente de très nombreux inconvénients, notamment éthiques...
Tipo: Text
Palavras-chave: Cytotoxicité; Toxicité sur larves de diptères; Activité antibactérienne; Bactéries marines; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00253/36470/35015.pdf

Stratégie de mesure non ciblée de type métabolomique (couplage LC-HRMS ) pour identifier différents groupes de composés bioactifs accumulés dans les mollusques bivalves
Provedor de dados: 5
Autores: Mondeguer, Florence; Antignac, Jean-philippe; Guitton, Yann; Monteau, Fabrice; Le Borgne, Sabrina; Hess, Philipp.
La bioactivité des toxines du phytoplancton qui s’accumulent dans les coquillages est presque toujours évaluée sur modèle animal (test souris). Malgré ses avantages, la capacité de ce test à expliquer la nature de cette bio-activité reste limitée. Par ailleurs, le contrôle sanitaire actuellement basé sur une méthode ciblée d’identification et de quantification d’un ensemble de toxines connues ne permet pas de détecter des toxines encore inconnues. Afin de répondre à ce besoin de caractérisation de substances toxiques inconnues, une nouvelle approche de profilage chimique différentiel et non ciblé, de type métabolomique, a été proposée. Les 2 extraits sélectionnés sont ceux ayant montré une toxicité positive chez la souris, sans que les substances...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00179/29042/27480.pdf

Azaspiracid shellfish poisoning: A review on the chemistry, ecology, and toxicology with an emphasis on human health impacts
Provedor de dados: 5
Autores: Twiner, Michael J.; Rehmann, Nils; Hess, Philipp; Doucette, Gregory J.
Azaspiracids (AZA) are polyether marine toxins that accumulate in various shellfish species and have been associated with severe gastrointestinal human intoxications since 1995. This toxin class has since been reported from several countries, including Morocco and much of western Europe. A regulatory limit of 160 mu g AZA/kg whole shellfish flesh was established by the EU in order to protect human health; however, in some cases, AZA concentrations far exceed the action level. Herein we discuss recent advances on the chemistry of various AZA analogs, review the ecology of AZAs, including the putative progenitor algal species, collectively interpret the in vitro and in vivo data on the toxicology of AZAs relating to human health issues, and outline the...
Tipo: Text
Palavras-chave: Azaspiracid (AZA); AZP; Shellfish poisoning.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00077/18805/16375.pdf

Chemically mediated interactions between Microcystis and Planktothrix: Impact on their physiology and metabolic profiles
Provedor de dados: 5
Autores: Briand, Enora; Reubrech, Sébastien; Mondeguer, Florence; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Bormans, Myriam.
Freshwater cyanobacteria are well known for their ability to produce a wide variety of bioactive compounds, some of which have been described as allelochemicals. There is growing evidence that these secondary metabolites play an important role in shaping community composition through biotic interactions; however, for the most part, their biological role and mode of regulation of the production are poorly understood. In temperate eutrophic freshwaters, Microcystis and Planktothrix often co-occur, with Planktothrix being an early colonizer and Microcystis appearing subsequently. We tested if the production of a range of peptides by co-existing species could be regulated through interspecific interactions. Using a combined approach of co-cultures and analyses...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00374/48515/48794.pdf

Production and Isolation of Azaspiracid-1 and -2 from Azadinium spinosum Culture in Pilot Scale Photobioreactors
Provedor de dados: 5
Autores: Jauffrais, Thierry; Kilcoyne, Jane; Sechet, Veronique; Herrenknecht, Christine; Truquet, Philippe; Herve, Fabienne; Berard, Jean-baptiste; Nulty, Ciara; Taylor, Sarah; Tillmann, Urban; Miles, Christopher O.; Hess, Philipp.
Azaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell·mL−1 at...
Tipo: Text
Palavras-chave: Solid phase extraction; Photobioreactor; Chemostat; Dinoflagellate; Micro-algae; LC-MS/MS; Tangential flow filtration; Azaspiracid; HP-20.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00088/19875/17526.pdf

High resolution mass spectrometry for quantitative analysis and untargeted screening of algal toxins in mussels and passive samplers
Provedor de dados: 5
Autores: Zendong, Suzie Zita; Mccarron, Pearse; Herrenknecht, Christine; Sibat, Manoella; Amzil, Zouher; Cole, Richard B.; Hess, Philipp.
Measurement of marine algal toxins has traditionally focussed on shellfish monitoring while, over the last decade, passive sampling has been introduced as a complementary tool for exploratory studies. Since 2011, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been adopted as the EU reference method (No.15/2011) for detection and quantitation of lipophilic toxins. Traditional LC-MS approaches have been based on low-resolution mass spectrometry (LRMS), however, advances in instrument platforms have led to a heightened interest in the use of high-resolution mass spectrometry (HRMS) for toxin detection. This work describes the use of HRMS in combination with passive sampling as a progressive approach to marine algal toxin surveys. Experiments...
Tipo: Text
Palavras-chave: Monitoring; Marine toxins; Passive sampling; SPATT; Matrix effects.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00277/38848/37388.pdf

Contribution of marine invertebrates to Ciguatera poisoning : the case study of French Polynesia
Provedor de dados: 5
Autores: Darius, Ht; Viallon, J; Gatti, Cm; Chinain, M; Roué, M; Sibat, Manoella; Amzil, Zouher; Hess, Philipp; Vandersea, Mw; Litaker, Rw; Tester, Pa.
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00450/56181/57729.pdf

First insight on metabolomics workflows and differences between dinoflagellate: characterization and discrimination of modern and ancient strains and their algal growth
Provedor de dados: 5
Autores: Mondeguer, Florence; Souard, Florence; Guitton, Yann; Latimier, Marie; Quere, Julien; Sibat, Manoella; Hess, Philipp; Siano, Raffaele.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a untargeted metabolomics approach by LC-HRMS to study the possible...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00440/55199/56664.pdf

The effects of growth phase and light intensity on toxin production by Dinophysis acuminata from the northeastern United States
Provedor de dados: 5
Autores: Tong, Mengmeng; Kulis, David M.; Fux, Elie; Smith, Juliette L.; Hess, Philipp; Zhou, Qixing; Anderson, Donald M.
For many years, the study of toxic Dinophysis species was primarily restricted to field populations until it was recently demonstrated that some of these organisms can be mixotrophically cultured in the laboratory with the ciliate prey, Myrionecta rubra. which had previously been fed with cryptophytes of the genus Teleaulax and Geminigera. Here we investigated the influence of growth phase and light intensity on the production of diarrhetic shellfish poisoning (DSP) toxins and pectenotoxins (PTXs) in cultures of Dinophysis acuminata from the northeastern United States. The cell toxin content of okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-2 (PTX2), and the okadaic acid diol ester (OA-D8) varied significantly with growth phase under all light...
Tipo: Text
Palavras-chave: Diarrhetic shellfish poisoning (DSP); Dinophysis acuminata; Dinophysistoxin (DTX); Growth phase; Light; Okadaic acid (OA); Pectenotoxins (PTXs).
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00037/14827/12195.pdf

Toxicological Investigations on the Sea Urchin Tripneustes gratilla (Toxopneustidae, Echinoid) from Anaho Bay (Nuku Hiva, French Polynesia): Evidence for the Presence of Pacific Ciguatoxins
Provedor de dados: 5
Autores: Darius, Helene Taiana; Roue, Melanie; Sibat, Manoella; Viallon, Jerome; Gatti, Clemence Mahana Iti; Vandersea, Mark W.; Tester, Patricia A.; Litaker, R. Wayne; Amzil, Zouher; Hess, Philipp; Chinain, Mireille.
The sea urchin Tripneustes gratilla (Toxopneustidae, Echinoids) is a source of protein for many islanders in the Indo-West Pacific. It was previously reported to occasionally cause ciguatera-like poisoning; however, the exact nature of the causative agent was not confirmed. In April and July 2015, ciguatera poisonings were reported following the consumption of T. gratilla in Anaho Bay (Nuku Hiva Island, Marquesas archipelago, French Polynesia). Patient symptomatology was recorded and sea urchin samples were collected from Anaho Bay in July 2015 and November 2016. Toxicity analysis using the neuroblastoma cell–based assay (CBA-N2a) detected the presence of ciguatoxins (CTXs) in T. gratilla samples. Gambierdiscus species were predominant in the benthic...
Tipo: Text
Palavras-chave: Ciguatera poisoning; Ciguatoxins; Tripneustes gratilla; Sea urchin; Echinoidea; Gambierdiscus polynesiensis; Windowscreens; Artificial substrates; QPCR assays; CBA-N2a; LC-MS/MS.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00436/54716/56153.pdf

Identification of paralytic shellfish poisons using liquid chromatography / ion mobility - high resolution mass spectrometry
Provedor de dados: 5
Autores: Poyer, Salomé; Loutelier-bourhis, Corinne; Mondeguer, Florence; Coadou, Gaël; Hubert-roux, Marie; Enche, Julien; Bossee, Anne; Hess, Philipp; Afonso, Carlos.
Saxitoxin and its analogues also called paralytic shellfish poisons (PSPs) are very potent neurotoxins [1] produced by dinoflagellates and referenced as chemical weapon in the chemical warfare convention (CWC). The official detection methods for these toxins present limitations concerning their speed and reliability [2].  Due to the presence of isomers, not differentiable by mass spectrometry (MS), an upstream separation is necessary. In order to separate saxitoxin analogues, hydrophilic interaction liquid chromatography (HILIC) and ion mobility (IM) were used. Those techniques respectively developed for high polar compounds and three dimensional structure differentiation are particularly well adapted to the separation of PSPs. This HILIC/IM-MS coupling...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00179/29039/27475.pdf

Identification and separation of saxitoxins using hydrophilic interaction liquid chromatography coupled to traveling wave ion mobility-mass spectrometry
Provedor de dados: 5
Autores: Poyer, Salome; Loutelier-bourhis, Corinne; Coadou, Gael; Mondeguer, Florence; Enche, Julien; Bossee, Anne; Hess, Philipp; Afonso, Carlos.
The aim of this work was to develop a reliable and efficient analytical method to characterise and differentiate saxitoxin analogues (STX), including sulphated (gonyautoxins, GTX) and non-sulphated analogues. For this purpose, hydrophilic interaction liquid chromatography (HILIC) was used to separate sulphated analogues. We also resorted to ion mobility spectrometry to differentiate the STX analogues because this technique adds a new dimension of separation based on ion gas phase conformation. Positive and negative ionisation modes were used for gonyautoxins while positive ionisation mode was used for non-sulphated analogues. Subsequently, the coupling of these three complementary techniques, HILIC-IM-MS, permitted the separation and identification of STX...
Tipo: Text
Palavras-chave: Saxitoxin; Ion mobility-mass spectrometry; Isomers; Multidimensional separation; HILIC.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00249/36067/34924.pdf

Human Health and Ocean Pollution
Provedor de dados: 5
Autores: Landrigan, Philip J.; Stegeman, John J.; Fleming, Lora E.; Allemand, Denis; Anderson, Donald M.; Backer, Lorraine C.; Brucker-davis, Françoise; Chevalier, Nicolas; Corra, Lilian; Czerucka, Dorota; Bottein, Marie-yasmine Dechraoui; Demeneix, Barbara; Depledge, Michael; Deheyn, Dimitri D.; Dorman, Charles J.; Fénichel, Patrick; Fisher, Samantha; Gaill, Françoise; Galgani, Francois; Gaze, William H.; Giuliano, Laura; Grandjean, Philippe; Hahn, Mark E.; Hamdoun, Amro; Hess, Philipp; Judson, Bret; Laborde, Amalia; Mcglade, Jacqueline; Mu, Jenna; Mustapha, Adetoun; Neira, Maria; Noble, Rachel T.; Pedrotti, Maria Luiza; Reddy, Christopher; Rocklöv, Joacim; Scharler, Ursula M.; Shanmugam, Hariharan; Taghian, Gabriella; Van De Water, Jeroen A. J. M.; Vezzulli, Luigi; Weihe, Pál; Zeka, Ariana; Raps, Hervé; Rampal, Patrick.
Background: Pollution – unwanted waste released to air, water, and land by human activity – is the largest environmental cause of disease in the world today. It is responsible for an estimated nine million premature deaths per year, enormous economic losses, erosion of human capital, and degradation of ecosystems. Ocean pollution is an important, but insufficiently recognized and inadequately controlled component of global pollution. It poses serious threats to human health and well-being. The nature and magnitude of these impacts are only beginning to be understood. Goals: (1) Broadly examine the known and potential impacts of ocean pollution on human health. (2) Inform policy makers, government leaders, international organizations, civil society, and the...
Tipo: Text
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00662/77369/78974.pdf

Effects of pH and Nutrients (Nitrogen) on Growth and Toxin Profile of the Ciguatera-Causing Dinoflagellate Gambierdiscus polynesiensis (Dinophyceae)
Provedor de dados: 5
Autores: Longo, Sébastien; Sibat, Manoella; Taiana Darius, Hélène; Hess, Philipp; Chinain, Mireille.
Ciguatera poisoning is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. Ciguatera outbreaks are expected to increase worldwide with global change, in particular as a function of its main drivers, including changes in sea surface temperature, acidification, and coastal eutrophication. In French Polynesia, G. polynesiensis is regarded as the dominant source of CTXs entering the food web. The effects of pH (8.4, 8.2, and 7.9), Nitrogen:Phosphorus ratios (24N:1P vs. 48N:1P), and nitrogen source (nitrates vs. urea) on growth rate, biomass, CTX levels, and profiles were examined in four clones of G. polynesiensis at different culture age (D10, D21,...
Tipo: Text
Palavras-chave: Gambierdiscus polynesiensis; French Polynesia; Ciguatera; Ciguatoxins; LC-MS; MS; Toxin profile; Nitrate; Urea; Culture medium acidification.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00662/77382/78981.pdf

Relative Molar Response of lipophilic marine algal toxins in liquid chromatography electrospray ionization mass spectrometry
Provedor de dados: 5
Autores: Zendong, Suzie Zita; Sibat, Manoella; Herrenknecht, Christine; Hess, Philipp; Mccarron, Pearse.
Rationale Accurate quantitative analysis of lipophilic toxins by liquid chromatography-mass spectrometry (LC-MS) requires calibration solution reference materials (RMs) for individual toxin analogs. Untargeted analysis is aimed at identifying a vast number of compounds and thus validation of fully quantitative untargeted methods is not feasible. However, a semi-quantitative approach allowing for profiling is still required and will be strengthened by knowledge of the relative molar response (RMR) of analogs in liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization (ESI). Methods RMR factors were evaluated for toxins from the okadaic acid (OA/DTXs), yessotoxin (YTX), pectenotoxin (PTX), azaspiracid (AZA) and cyclic imine (CI) toxin...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00387/49877/50434.pdf

Systematic detection of BMAA (β-N-methylamino-L-alanine) and DAB (2,4-diaminobutyric acid) in mollusks collected in shellfish production areas along the French coasts
Provedor de dados: 5
Autores: Reveillon, Damien; Sechet, Veronique; Hess, Philipp; Amzil, Zouher.
The neurotoxin β-N-methylamino-L-alanine (BMAA) is naturally present in some microalgal species in the marine environment. The accumulation of BMAA has widely been observed in filter-feeding bivalves that are known to consume primary producers constituting the base of complex aquatic food webs. This study was performed to assess the occurrence of BMAA and isomers in mollusks collected from nine representative shellfish production areas located on the three French coasts (Channel, Atlantic and Mediterranean sites). The use of a highly selective and sensitive HILIC-MS/MS method, with D5DAB as internal standard, revealed the systematic detection of BMAA and DAB, in concentrations ranging from 0.20 to 6.7 μg g-1 dry weight of digestive gland tissues of...
Tipo: Text
Palavras-chave: BMAA; Chaetoceros; Thalassiosira; Phaeodactylum; Bivalves; Seafood.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00297/40780/39784.pdf

Harmful Algal Blooms (HABs) and Desalination: A Guide to Impacts, Monitoring and Management
Provedor de dados: 5
Autores: Hess, Philipp; Villacorte, Loreen O; Dixon, Mike B; Boerlage, Siobhan Fe; Anderson, Donald M; Kennedy, Maria D; Schippers, Jan C.
Once harmful algal blooms (HABs) reach a desalination plant, they can cause significant operational issues and potential health concerns for consumers. These issues stem from two factors – first, the algal cells produce organic matter that can cause filter clogging and membrane fouling, and secondly, some cells produce toxic substances or taste and odor compounds. This chapter first explains the mechanisms for cellular release of organic matter, the types of matter that are produced, and the relative contribution of each type of matter to fouling mechanisms. It then describes the wide range of toxins that are produced by HABs, their mode of toxicity, and analytical methods for detecting them. While taste and odor compounds are non-toxic, they are included...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00407/51837/52438.pdf

A mussel tissue certified reference material for multiple phycotoxins. Part 1: design and preparation
Provedor de dados: 5
Autores: Mccarron, Pearse; Emteborg, Hakan; Nulty, Ciara; Rundberget, Thomas; Loader, Jared I.; Teipel, Katharina; Miles, Christopher O.; Quilliam, Michael A.; Hess, Philipp.
The development of multi-analyte methods for lipophilic shellfish toxins based on liquid chromatography-mass spectrometry permits rapid screening and analysis of samples for a wide variety of toxins in a single run. Validated methods and appropriate certified reference materials (CRMs) are required to ensure accuracy of results. CRMs are essential for accurate instrument calibration, for assessing the complete analytical method from sample extraction to data analysis and for verifying trueness. However, CRMs have hitherto only been available for single toxin groups. Production of a CRM containing six major toxin groups was achieved through an international collaboration. Preparation of this material, CRM-FDMT1, drew on information from earlier studies as...
Tipo: Text
Palavras-chave: CRM-FDMT1; Certified reference material; Shellfish toxins; Phycotoxins; Accuracy; Precision; Liquid chromatography-mass spectrometry.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00037/14826/12237.pdf

Maitotoxin-4, a Novel MTX Analog Produced by Gambierdiscus excentricus
Provedor de dados: 5
Autores: Pisapia, Francesco; Sibat, Manoella; Herrenknecht, Christine; Lhaute, Korian; Gaiani, Greta; Ferron, Pierre-jean; Fessard, Valerie; Fraga, Santiago; Nascimento, Silvia M.; Litaker, R. Wayne; Holland, William C.; Roullier, Catherine; Hess, Philipp.
Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from...
Tipo: Text
Palavras-chave: Gambierdiscus excentricus; Maitotoxin-4; Bioguided fractionation; Size-exclusion chromatography (LH-20); Neuro-2a (N2a) assay; LC-HRMS/MS (Q-Tof 6550); LC-LRMS/MS (API4000 QTrap).
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00392/50320/51001.pdf

β-N-methylamino-l-alanine (BMAA) and isomers: Distribution in different food web compartments of Thau lagoon, French Mediterranean Sea
Provedor de dados: 5
Autores: Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Masseret, Estelle; Hess, Philipp; Amzil, Zouher.
The neurotoxin BMAA (β-N-methylamino-l-alanine) and its isomer DAB (2,4-diaminobutyric acid) have been detected in seafood worldwide, including in Thau lagoon (French Mediterranean Sea). A cluster of amyotrophic lateral sclerosis (ALS), a neurodegenerative disease associated with BMAA, has also been observed in this region. Mussels, periphyton (i.e. biofilms attached to mussels) and plankton were sampled between July 2013 and October 2014, and analyzed using HILIC-MS/MS. BMAA, DAB and AEG (N-(2-aminoethyl)glycine) were found in almost all the samples of the lagoon. BMAA and DAB were present at 0.58 and 0.83, 2.6 and 3.3, 4.0 and 7.2 μg g−1 dry weight in plankton collected with nets, periphyton and mussels, respectively. Synechococcus sp., Ostreococcus...
Tipo: Text
Palavras-chave: BMAA; Diatoms; Chaetoceros sp.; Seston; Mytilus galloprovincialis; HILIC-MS/MS.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00275/38607/37192.pdf

Innovative metabolomic workflows to study growth kinetics dinoflagellate cysts revived from modern and ancient sediments
Provedor de dados: 5
Autores: Mondeguer, Florence; Souard, Florence; Guitton, Yann; Allard, Pierre-marie; Elie, Nicolas; Sibat, Manoella; Latimier, Marie; Quere, Julien; Wolfender, Jean-luc; Siano, Raffaele; Hess, Philipp.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a metabolomic approach to study the possible adaptive responses of...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00464/57591/59769.pdf

Assessment of Ciguatera and Other Phycotoxin-Related Risks in Anaho Bay (Nuku Hiva Island, French Polynesia): Molecular, Toxicological, and Chemical Analyses of Passive Samplers
Provedor de dados: 5
Autores: Roué, Mélanie; Smith, Kirsty F.; Sibat, Manoella; Viallon, Jérôme; Henry, Kévin; Ung, André; Biessy, Laura; Hess, Philipp; Darius, Hélène Taiana; Chinain, Mireille.
Ciguatera poisoning is a foodborne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates from the genera Gambierdiscus and Fukuyoa. The suitability of Solid Phase Adsorption Toxin Tracking (SPATT) technology for the monitoring of dissolved CTXs in the marine environment has recently been demonstrated. To refine the use of this passive monitoring tool in ciguateric areas, the effects of deployment time and sampler format on the adsorption of CTXs by HP20 resin were assessed in Anaho Bay (Nuku Hiva Island, French Polynesia), a well-known ciguatera hotspot. Toxicity data assessed by means of the mouse neuroblastoma cell-based assay (CBA-N2a) showed that a 24 h deployment of 2.5 g of resin allowed...
Tipo: Text
Palavras-chave: Ciguatera monitoring; Gambierdiscus; Ciguatoxins; SPATT passive samplers; HP20 resin; CBA-N2a; LC-MS; MS; WS artificial substrate; QPCR; HTS metabarcoding.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00629/74143/73670.pdf

Nouvelle stratégie de caractérisation non ciblée de type métabolomique au service de l’identification de composés bioactifs accumulés dans les mollusques bivalves
Provedor de dados: 5
Autores: Mondeguer, Florence; Antignac, Jean-philippe; Guitton, Yann; Monteau, Fabrice; Le Borgne, Sabrina; Hess, Philipp.
The bioactivity of phytoplankton toxins that accumulate in shellfish is almost always tested on animal model (mice assay). In spite of these advantages, the ability of this test to explain the nature of the bioactivity remains limited. Besides, the current sanitary control based on targeted methods of identification and quantification (LC-MS/MS) of known toxins, do not permit to detect unknown toxins. In order to cope with this need of identification of unknown toxic substances, a next approach based on global and differential metabolomic profiling was proposed. The first results obtained from extracts that have shown a positive toxicity in mice without the substances potentially responsible for these toxic effects have been measured by targeted methods,...
Tipo: Text
Palavras-chave: Métabolomique; LC-HRMS; Biotoxines marines; Biomarqueur.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00077/18784/16352.pdf

Preliminary metabolomic approach on cyanobacterial co-cultures: Chemically mediated interactions between Microcystis and Planktothrix
Provedor de dados: 5
Autores: Mondeguer, Florence; Sibat, Manoella; Reubrecht, Sébastien; Amzil, Zouher; Bormans, Myriam; Hess, Philipp; Briand, Enora.
Cyanobacterial proliferation is one of the most harmful hazards, in both freshwater and marine ecosystems. Cyanobacteria are well known for their ability to produce a wide variety of bioactive compounds, some of which have been described as allelochemicals. There is growing evidence that these secondary metabolites play an important role in shaping community composition through biotic interactions; however, for the most part, their biological role and mode of regulation of the production are poorly understood. In temperate eutrophic freshwaters, Microcystis and Planktothrix often co-occur, with Planktothrix being an early colonizer and Microcystis appearing subsequently. By integrating LC-MS/MS molecular networking and an innovative experimental design, we...
Tipo: Text
Palavras-chave: Cyanobacteria; Cyanotoxins; Plankton interactions; Co_culture; LC_HRMS; Molecular Network; Metabolomics.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00405/51666/52215.pdf

Chemically mediated interactions between   Microcystis   and   Planktothrix   : impact on their growth, morphology and metabolic profiles
Provedor de dados: 5
Autores: Briand, Enora; Reubrecht, Sébastien; Mondeguer, Florence; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Bormans, Myriam.
Freshwater cyanobacteria are known for their ability to produce bioactive compounds, some of which have been described as allelochemicals. Using a combined approach of co‐cultures and analyses of metabolic profiles, we investigated chemically mediated interactions between two cyanobacterial strains, Microcystis aeruginosa PCC7806 and Planktothrix agardhii PCC7805. More precisely, we evaluated changes in growth, morphology and metabolite production and release by both interacting species. Co‐culture of Microcystis with Planktothrix resulted in a reduction of the growth of Planktothrix together with a decrease of its trichome size and alterations in the morphology of its cells. The production of intracellular compounds by Planktothrix showed a slight...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00470/58152/60632.pdf

Metabolomic approach for the analysis of micro-algae : direct analysis versus passive sampling
Provedor de dados: 5
Autores: Zendong, Suzie Zita; Herrenknecht, Christine; Mondeguer, Florence; Hess, Philipp.
By 2014, the new targeted LC-MS/MS reference method for the detection of lipophilic toxins will replace the mouse bioassay (MBA). This bioassay, which has the advantage of being a rapid and global toxicity test, can not be used for toxin identification purposes. Furthermore, it has appeared that, in some cases, the mouse bioassay could reveal uncharacterized toxicities that could not be elucidated by targeted mass spectrometry methods. Therefore, moving from the global toxicity assessment test to a targeted technique leads to a lack of information on emerging toxins. Objectives: - Beside targeted methods, develop metabolomic approaches to screen for known and unknown emerging toxins - Develop passive sampling devices suitable for toxins of varying...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00179/29040/27478.pdf

Use of Mass Spectrometry to Determine the Diversity of Toxins Produced by Gambierdiscus and Fukuyoa Species from Balearic Islands and Crete (Mediterranean Sea) and the Canary Islands (Northeast Atlantic)
Provedor de dados: 5
Autores: Estevez, Pablo; Sibat, Manoella; Leão-martins, José Manuel; Tudó, Angels; Rambla-alegre, Maria; Aligizaki, Katerina; Diogène, Jorge; Gago-martinez, Ana; Hess, Philipp.
Over the last decade, knowledge has significantly increased on the taxonomic identity and distribution of dinoflagellates of the genera Gambierdiscus and Fukuyoa. Additionally, a number of hitherto unknown bioactive metabolites have been described, while the role of these compounds in ciguatera poisoning (CP) remains to be clarified. Ciguatoxins and maitotoxins are very toxic compounds produced by these dinoflagellates and have been described since the 1980s. Ciguatoxins are generally described as the main contributors to this food intoxication. Recent reports of CP in temperate waters of the Canary Islands (Spain) and the Madeira archipelago (Portugal) triggered the need for isolation and cultivation of dinoflagellates from these areas, and their...
Tipo: Text
Palavras-chave: Maitotoxins; Ciguatoxins; Gambierdiscus; Fukuyoa; LC-MS; MS; HRMS; QToF; Ciguatera poisoning.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00629/74081/73530.pdf

First metabolomic approach of the epiphytic bacteria-marine diatom Haslea ostrearia relationships
Provedor de dados: 5
Autores: Mondeguer, Florence; Lepinay, Alexandra; Capiaux, Hervé; Turpin, Vincent; Baron, Regis; Hess, Philipp; Lebeau, Thierry; Boug,.
The marine diatom Haslea ostrearia [1] produces a water-soluble blue-pigment named marennine [2] of economic interest. But the lack of knowledge of the ecological conditions, under which this microalga develops in its natural ecosystem, more especially bacteria H. ostrearia interactions, prevents any optimization of its culture in well-controlled conditions.  The structure of the bacterial community was analyzed by PCR-TTGE before and after the isolation of H. ostrearia cells recovered from 4 localities, to distinguish the relative part of the biotope and the biocenose and eventually to describe the temporal dynamic of the structure of the bacterial community at two time-scales. The differences in genetic fingerprints, more especially high between two H....
Tipo: Text
Palavras-chave: Haslea ostrearia; Marine diatom; Co-culture microalgae; Bacterial community; PCR-TTGE; High Resolution Mass Spectrometry; Untargeted metabolomics.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00342/45367/44862.pdf

Intoxikation durch marine Biotoxine
Provedor de dados: 5
Autores: Hess, Philipp.
This paper presents a short summary of the knowledge on marine biotoxins. As toxins are known for their acute effects, they have been classified here according to the effects they cause in acute human poisoning incidents. Toxins may thus be distinguished into those that affect the nervous system (paralytic and other neurotoxins), memory (amnesic poisons), and the digestive system (diarrhetic toxins). Furthermore, newly emerging toxins, such as ciguatoxins or shark toxins, and factors that lead to the introduction of toxins in new areas, are presented. Relevant suggestions for further reading are given.
Tipo: Text
Palavras-chave: Seafood; Poisoning; Paralysis; Amnesia; Diarrhoea.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00385/49671/50210.pdf

Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis
Provedor de dados: 5
Autores: Roue, Melanie; Darius, Helene Taiana; Ung, Andre; Viallon, Jerome; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Chinain, Mireille.
Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were...
Tipo: Text
Palavras-chave: Giant clams; Ex situ exposure to toxic algae; Ciguatoxins; Gambierdiscus polynesiensis; Anatomical distribution; Toxin elimination; CBA-N2a; LC-MS/MS.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00440/55123/56579.pdf

Effect of Nitrate, Ammonium and Urea on Growth and Pinnatoxin G Production of Vulcanodinium rugosum
Provedor de dados: 5
Autores: Abadie, Eric; Kaci, Lamia; Berteaux, Tom; Hess, Philipp; Sechet, Veronique; Masseret, Estelle; Rolland, Jean-luc; Laabir, Mohamed.
ulcanodinium rugosum, a recently described dinoflagellate species producing a potent neurotoxin (pinnatoxin G), has been identified in French Mediterranean lagoons and was responsible for recurrent episodes of shellfish toxicity detected by mouse bioassay. Until now, the biology and physiology of V. rugosum have not been fully investigated. We studied the growth characteristics and toxicity of a V. rugosum strain (IFR-VRU-01), isolated in the Ingril lagoon in June 2009 (North-Western French Mediterranean Sea). It was cultivated in Enriched Natural Sea Water (ENSW) with organic (urea) and inorganic (ammonium and nitrate) nitrogen, at a temperature of 25 °C and irradiance of 100 μmol/m2·s−1. Results showed that ammonium was assimilated by cells more rapidly...
Tipo: Text
Palavras-chave: Vulcanodinium rugosum; Pinnatoxin G; Ingril lagoon; Growth; Nitrogen source.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00276/38748/37290.pdf

Effect of Azadinium spinosum on the feeding behaviour and azaspiracid accumulation of Mytilus edulis
Provedor de dados: 5
Autores: Jauffrais, Thierry; Contreras, Andrea; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Tillmann, Urban; Hess, Philipp.
Azadinium spinosum, a small toxic dinoflagellate, was recently isolated and identified as a primary producer of azaspiracid toxins (AZAs). Previous experiments related to AZA accumulation in blue mussels upon direct feeding with A. spinosum revealed increased mussel mortality and had negative effects on the thickness of the digestive gland tubules. Therefore we conducted follow up experiments in order to study effects of A. spinosum on mussel feeding behaviour. Individual assessment of mussel feeding time activity (FTA), clearance rate (CR), filtration rate (TFR), absorption rate (AR), faeces and pseudofaeces production were carried out on mussel fed either toxic (A. spinosum) or non-toxic (Isochrisis aff. galbana (T-Iso)) diets. Furthermore, AZA...
Tipo: Text
Palavras-chave: Bivalve molluscs; Mussel; Ecophysiology; AZA biotransformation; AZA accumulation; Trophic transfer; Dinoflagellate; Azaspiracid.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00090/20174/17832.pdf

Influence of temperature, irradiance and pH on the physiology of Teleaulax amphioxeia
Provedor de dados: 5
Autores: Gaillard, Sylvain; Charrier, Aurelie; Carpentier, Liliane; Malo, Florent; Bougaran, Gael; Hegaret, Helene; Hess, Philipp; Sechet, Veronique.
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00617/72958/71990.pdf

Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers
Provedor de dados: 5
Autores: Geiger, Marie; Guitton, Yann; Vansteelandt, M.; Kerzaon, I.; Blanchet, Estelle; Du Pont, T. Robiou; Frisvad, J. C.; Hess, Philipp; Pouchus, Y. F.; Grovel, Olivier.
In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with...
Tipo: Text
Palavras-chave: Cytotoxicity; Dereplication; Marine fungi; Mass spectrometry; Penicillium; Shellfish.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00160/27166/25523.pdf

Contributions to the characterisation of risks posed by marine biotoxins
Provedor de dados: 5
Autores: Hess, Philipp.
Toxic algae producing an array of bioactive compounds may accumulate in shellfish, which in turn cause poisoning, following human consumption of contaminated shellfish. The chemistry, occurrence and toxicity of the toxins involved was reviewed. Following this review, the risk analysis and management processes are outlined for the production of shellfish. Subsequent sections describe five elements of risk characterisation to which my studies have significantly contributed: methods of analysis, distribution of toxins in the marine environment, preparative isolation of toxins, quality control tools in the determination of the toxins and characterisation of the chemical hazard in terms of lipophilicity, stability, reactivity and toxicity.  The use of liquid...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12661/9548.pdf

Structure Elucidation, Relative LC–MS Response and In Vitro Toxicity of Azaspiracids 7–10 Isolated from Mussels (Mytilus edulis)
Provedor de dados: 5
Autores: Kilcoyne, Jane; Twiner, Michael J.; Mccarron, Pearse; Crain, Sheila; Giddings, Sabrina D.; Foley, Barry; Rise, Frode; Hess, Philipp; Willdns, Alistair L.; Miles, Christopher O.
Azaspiracids (AZAs) are marine biotoxins produced by dinoflagellates that can accumulate in shellfish, which if consumed can lead to poisoning events. AZA7–10, 7–10, were isolated from shellfish and their structures, previously proposed on the basis of only LC–MS/MS data, were confirmed by NMR spectroscopy. Purified AZA4–6, 4–6, and 7–10 were accurately quantitated by qNMR and used to assay cytotoxicity with Jurkat T lymphocyte cells for the first time. LC–MS(MS) molar response studies performed using isocratic and gradient elution in both selected ion monitoring and selected reaction monitoring modes showed that responses for the analogues ranged from 0.3 to 1.2 relative to AZA1, 1. All AZA analogues tested were cytotoxic to Jurkat T lymphocyte cells in a...
Tipo: Text
Palavras-chave: Azaspiracid; Structure confirmation; LC-MS molar response; NMR; Mass spectrometry; Purification; Jurkat T; Toxicity.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/38059/36190.pdf

Tectus niloticus (Tegulidae, Gastropod) as a Novel Vector of Ciguatera Poisoning: Detection of Pacific Ciguatoxins in Toxic Samples from Nuku Hiva Island (French Polynesia)
Provedor de dados: 5
Autores: Darius, Helene Taiana; Roue, Melanie; Sibat, Manoella; Viallon, Jerome; Gatti, Clemence Mahana Iti; Vandersea, Mark W.; Tester, Patricia A.; Litaker, R. Wayne; Amzil, Zouher; Hess, Philipp; Chinain, Mireille.
Ciguatera fish poisoning (CFP) is a foodborne disease caused by the consumption of seafood (fish and marine invertebrates) contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genus Gambierdiscus. The report of a CFP-like mass-poisoning outbreak following the consumption of Tectus niloticus (Tegulidae, Gastropod) from Anaho Bay on Nuku Hiva Island (Marquesas archipelago, French Polynesia) prompted field investigations to assess the presence of CTXs in T. niloticus. Samples were collected from Anaho Bay, 1, 6 and 28 months after this poisoning outbreak, as well as in Taiohae and Taipivai bays. Toxicity analysis using the neuroblastoma cell-based assay (CBA-N2a) detected the presence of CTXs only in Anaho Bay T. niloticus samples. This is...
Tipo: Text
Palavras-chave: Ciguatera poisoning; Ciguatoxins; Tectus niloticus; Gastropods; Gambierdiscus polynesiensis; QPCR assays; CBA-N2a; LC-MS; MS.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00426/53798/54719.pdf

Harmful Algae 2018 – From Ecosystems to Socioecosystems. Proceedings of the 18th International Conference on Harmful Algae, 21-26 October 2018, Nantes, France
Provedor de dados: 5
Autores: Hess, Philipp.
The 18th International Conference on Harmful Algae (ICHA) was held at La Cité des Congrès, Nantes, France, 21-26 October 2018. Philipp Hess, editor of the proceedings, chaired the local organizing committee with the support of the French GdR PHYCOTOX and GIS Cyano societies. The theme of the conference “From Ecosystems to Socio-ecosystems” was chosen to stress the need to integrate natural sciences with social sciences to increase the impact of HAB science on society. This edition has enjoyed record attendance (709 participants from 64 countries) and scientific contributions (613 abstracts, with 255 oral presentations and 358 posters, 45 of which were also presented as ignite talks). It consisted in nine plenary lectures and 246 other lectures subdivided...
Tipo: Text
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00651/76285/77274.pdf

Pinnatoxines en lien avec l’espèce Vulcanodinium rugosum (II)
Provedor de dados: 5
Autores: Mondeguer, Florence; Abadie, Eric; Herve, Fabienne; Bardouil, Michele; Sechet, Veronique; Raimbault, Virginie; Berteaux, Tom; Zendong, Suzie Zita; Palvadeau, Hubert; Amzil, Zouher; Hess, Philipp; Fessard, Valérie; Huguet, Antoine; Sosa, Silvio; Tubaro, Aurelia; Aráoz, Rómulo; Molgó, Jordi.
Pour tenter d’apporter des réponses aux questions soulevées par une précédente étude commanditée pour approfondir les connaissances sur Vulcanodinium rugosum, l’organisme producteur de la Pinnatoxine G (PnTX G) (Hess et al., 2012), les objectifs de cette nouvelle étude sont les suivants : - Elargissement de la zone initiale de prélèvement d’Ingril à toute la lagune méditerranéenne et mise à profit de l’augmentation du nombre d’échantillons pour vérifier l’impact de l’effet matrice dans l’analyse LC-SM/MS. - Mise en culture en masse de Vulcanodinium rugosum et - Conduite d’une expérience de contamination in vivo sur des moules pour vérifier l’accumulation de la PnTX-G et comprendre les mécanismes de la métabolisation des PnTXs et de ses composés associés.....
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00285/39635/38126.pdf

Differentiation of gonyautoxins by ion mobility - mass spectrometry; a cationization study
Provedor de dados: 5
Autores: Poyer, Salome; Loutelier-bourhis, Corinne; Tognetti, Vincent; Joubert, Laurent; Enche, Julien; Bossee, Anne; Mondeguer, Florence; Hess, Philipp; Afonso, Carlos.
Gonyautoxins are potent natural neurotoxic analogues of saxitoxin. Due to their biological activity and submilligram lethal dose for man, fast and efficient methods are required for their characterization. Recent advances in ion mobility-mass spectrometry (IM-MS) showed that differentiation of isomers could be achieved using specific experimental conditions involving particular buffer gases as well as cationic species. In this work, IM-MS experiments were carried out using alkali metal ions (Met = Li+, Na+ or K+) and different buffer gases (N2, N2O and CO2) to improve the differentiation of gonyautoxin isomers. The separation of [GTX + Met]+ ion was achieved for GTX2/3 and GTX1/4 from their Na or K adducts. For dcGTX2/3, the ion mobility separation can...
Tipo: Text
Palavras-chave: Ion mobility; Gonyautoxin; Collision cross-section; Alkali adduct.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00317/42863/42291.pdf

Highlights of the XVII International Conference on Harmful Algae
Provedor de dados: 5
Autores: Reguera, Beatriz; Wells, Mark; Zingone, Adriana; Fraga, Santiago; Azavedo, S.; Magalhaes, V.; Jenkinson, Ian; Rodriguez-hernandez, Francisco; Anderson, Don M.; Hallegaeff, Gustaaf; Davidson, Keith; Hess, Philipp; Hegaret, Helene.
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00621/73293/72481.pdf

Azaspiracids Increase Mitochondrial Dehydrogenases Activity in Hepatocytes: Involvement of Potassium and Chloride Ions
Provedor de dados: 5
Autores: Pelin, Marco; Kilcoyne, Jane; Florio, Chiara; Hess, Philipp; Tubaro, Aurelia; Sosa, Silvio.
Background: Azaspiracids (AZAs) are marine toxins that are produced by Azadinium and Amphidoma dinoflagellates that can contaminate edible shellfish inducing a foodborne poisoning in humans, which is characterized by gastrointestinal symptoms. Among these, AZA1, -2, and -3 are regulated in the European Union, being the most important in terms of occurrence and toxicity. In vivo studies in mice showed that, in addition to gastrointestinal effects, AZA1 induces liver alterations that are visible as a swollen organ, with the presence of hepatocellular fat droplets and vacuoles. Hence, an in vitro study was carried out to investigate the effects of AZA1, -2, and -3 on liver cells, using human non-tumor IHH hepatocytes. Results: The exposure of IHH cells to...
Tipo: Text
Palavras-chave: Azaspiracids; Hepatocytes; Mitochondrial activity; Mechanism of toxicity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00494/60526/63989.pdf

Requirements for screening and confirmatory methods for the detection and quantification of marine biotoxins in end-product and official control
Provedor de dados: 5
Autores: Hess, Philipp.
An overview is given of the biological origin of phycotoxins, as well as their chemical characteristics. Major poisoning types are described and examples of poisoning events are given to illustrate the importance of the phenomenon to both shellfish consumers and the shellfish producing industry. The characteristics of phycotoxins as natural products, the lack of predictability of their occurrence, economic drivers and the freshness of shellfish consumed in many countries result in a number of requirements for methods to be used in the efficient detection of these compounds. Subsequently, the performance of mouse bioassays and mass spectrometry as detection tools are compared for examples from Irish and French monitoring programmes to assess the usefulness...
Tipo: Text
Palavras-chave: Bioanalytical methods; Bioassays; Mass spectrometry; Liquid chromatography; Biological samples.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00006/11767/8989.pdf

Recent developments in the detection of phycotoxins
Provedor de dados: 5
Autores: Hess, Philipp; Nicolau, Elodie.
Over the past seven years, methods available for the detection of phycotoxins have been extensively reviewed in a number of international expert committees, such as the consultations organised by FAO/IOC/WHO and EFSA, as well as by individual scientists. These reviews have shown that the methods available have severe limitations for the use in official control, either due to their limited scope and detection capability or due to a lack of calibration standards, reference materials and validation efforts. The present review focuses on recent developments in the detection of phycotoxins in several areas of applied research. Not being able to exhaustively describe all recent developments, the review focussed on three areas of interest to the authors: (i)...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00019/12975/9949.pdf

Fatty acid ester metabolites of gymnodimine in shellfish collected from China and in mussels (Mytilus galloprovincialis) exposed to Karenia selliformis
Provedor de dados: 5
Autores: Ji, Ying; Che, Yijia; Wright, Elliott J.; Mccarron, Pearse; Hess, Philipp; Li, Aifeng.
Marine shellfish exposed to the microalgae Karenia selliformis can accumulate gymnodimines (GYM). Shellfish samples collected from Beihai City in Guangxi Autonomous Region, and Ningde City in Fujian Province, in the South China Sea, as well as mussels Mytilus galloprovincialis fed on K. selliformis under laboratory conditions were analyzed. Gymnodimines and various fatty acid ester metabolites were detected in the clam Antigona lamellaris and pen shell Atrina pectinata, while no esters were found in the oyster Crassostrea sp. and the gastropod Batillaria zonalis despite positive detection of free GYM in both species. When present, the predominant acyl esters observed were 18:0-GYM-A and 20:1-GYM-A. Under laboratory conditions GYM-A was accumulated and...
Tipo: Text
Palavras-chave: Gymnodimines (GYM); Fatty acid ester; Karenia selliformis; Toxin profiles; Antioxidant enzymes.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00609/72153/73203.pdf

Ostreopsis cf. ovata (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil
Provedor de dados: 5
Autores: Nascimento, Silvia M; Neves, Raquel Af; De'Carli, Gabriela Al; Borsato, Geovanna T; Da Silva, Rodrigo Af; Melo, Guilherme A; De Morais, Agatha M; Cockell, Thais C; Fraga, Santiago; Menezes-salgueiro, Adriana D; Mafra, Luis L Jr; Hess, Philipp; Salgueiro, Fabiano.
Recurrent blooms of Ostreopsis cf. ovata have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1–D3 and D8–D10 regions of the LSU rDNA and ITS loci, and the morphology of O. cf. ovata isolates and field populations from locations along the Brazilian tropical and subtropical coastal regions and three oceanic islands are presented. Additional ITS sequences from three single cells from the tropical coast are provided. Toxin profiles and quantities of PLTX and their analogues; OVTXs; contained in cells from two clonal cultures and two field blooms from Rio de Janeiro were investigated. Morphology was examined using both light and epifluorescence microscopy....
Tipo: Text
Palavras-chave: Benthic dinoflagellates; Harmful algal blooms; Ovatoxins; Taxonomy; Morphometry.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00604/71594/70026.pdf

Complex Toxin Profile of French Mediterranean Ostreopsis cf. ovata Strains, Seafood Accumulation and Ovatoxins Prepurification
Provedor de dados: 5
Autores: Brissard, Charline; Herrenknecht, Christine; Sechet, Veronique; Herve, Fabienne; Pisapia, Francesco; Harcouet, Jocelyn; Lemee, Rodolphe; Chomerat, Nicolas; Hess, Philipp; Amzil, Zouher.
Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis assay. Results obtained with these two methods were comparable, suggesting ovatoxins...
Tipo: Text
Palavras-chave: Ostreopsis cf. ovata; Palytoxin &amp; ovatoxins; Culture; Seafood contamination; LC-MS/MS; Hemolysis assay; Mediterranean Sea.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00199/31025/29428.pdf

Ciguatoxicity of Gambierdiscus and Fukuyoa species from the Caribbean and Gulf of Mexico
Provedor de dados: 5
Autores: Litaker, R. Wayne; Holland, William C.; Hardison, D. Ransom; Pisapia, Francesco; Hess, Philipp; Kibler, Steven R.; Tester, Patricia A.
Dinoflagellate species belonging to the genera Gambierdiscus and Fukuyoa produce ciguatoxins (CTXs), potent neurotoxins that concentrate in fish causing ciguatera fish poisoning (CFP) in humans. While the structures and toxicities of ciguatoxins isolated from fish in the Pacific and Caribbean are known, there are few data on the variation in toxicity between and among species of Gambierdiscus and Fukuyoa. Quantifying the differences in species-specific toxicity is especially important to developing an effective cell-based risk assessment strategy for CFP. This study analyzed the ciguatoxicity of 33 strains representing seven Gambierdiscus and one Fukuyoa species using a cell based Neuro-2a cytotoxicity assay. All strains were isolated from either the...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00407/51839/52439.pdf

Combined oral toxicity of azaspiracid-1 and yessotoxin in female NMRI mice
Provedor de dados: 5
Autores: Aasen, John A. B.; Espenes, Arild; Miles, Christopher O.; Samdal, Ingunn A.; Hess, Philipp; Aune, Tore.
For many years, the presence of yessotoxins (YTXs) in shellfish has contributed to the outcome of the traditional mouse bioassay and has on many occasions caused closure of shellfisheries. Since YTXs do not appear to cause diarrhoea in man and exert low oral toxicity in animal experiments, it has been suggested that they should be removed from regulation. Before doing so, it is important to determine whether the oral toxicity of YTXs is enhanced when present together with shellfish toxins known to cause damage to the gastrointestinal tract. Consequently, mice were given high doses of YTX, at 1 or 5 mg/kg body weight, either alone or together with azaspiracid-1 (AZA1) at 200 μg/kg. The latter has been shown to induce damage to the small intestine at this...
Tipo: Text
Palavras-chave: Azaspiracid-1; AZA1; Yessotoxin; YTX; Marine algal toxins; Absorption; Pathology; Sublethal; NMRI; Mice; LC-MS/MS; Oral toxicity.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00034/14477/11776.pdf

Pinnatoxines en lien avec l’espèce Vulcanodinium rugosum
Provedor de dados: 5
Autores: Hess, Philipp; Herve, Fabienne; Abadie, Eric; Sechet, Veronique; Molgo, Jordi; Amzil, Zouher; Fessard, Valerie.
Suite à la découverte de la Pinnatoxine G comme agent responsable des toxicités atypiques à Ingril fin 2010, une étude a été commanditée afin d’approfondir les connaissances sur l’organisme producteur de cette toxine, Vulcanodinium rugosum, les toxines associées et leur toxicité.   L’organisme, étant considéré « cryptique » dû à sa faible présence dans le milieu, a été suivi de manière plus proche à Ingril. Il est apparu dans la colonne d’eau en juillet et août 2012, et quelques échantillons ont pu être prélevés pour la mise en culture de souches supplémentaires. Une corrélation directe entre la croissance de l’organisme et la température est soupçonnée mais n’a pas encore pu être corroborée sur la période d’étude, faute d’un nombre suffisant...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00094/20518/18190.pdf

Quantitative analysis of azaspiracids in Azadinium spinosum cultures
Provedor de dados: 5
Autores: Jauffrais, Thierry; Herrenknecht, Christine; Sechet, Veronique; Sibat, Manoella; Tillmann, Urban; Krock, Bernd; Kilcoyne, Jane; Miles, Christopher O.; Mccarron, Pearse; Amzil, Zouher; Hess, Philipp.
Azaspiracids (AZAs) are secondary metabolites of Azadinium spinosum, that have been shown to cause diarrhetic shellfish poisoning when accumulated in bivalve molluscs. We describe here an analytical procedure for the determination of AZAs in cultures of A. spinosum with a focus on the potential formation of AZA methyl-esters as artefacts in the extraction and sample pre-treatment. A. spinosum cells were collected from bioreactor cultures, using centrifugation or filtration. Different extraction procedures were evaluated for formation of methyl-ester artefacts, yield, and matrix effects. Filtration of cultures using glass-fibre filters led to increased formation of methyl-esters. Hence centrifugation is recommended for recovery of cells. The type of...
Tipo: Text
Palavras-chave: Extraction procedure; Extraction artefact; Matrix effects; LC-MS/MS; Azaspiracid methyl ester; Dinoflagellate; Liquid chromatography-mass spectrometry.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00077/18812/16463.pdf

Pinnatoxin G is responsible for atypical toxicity in mussels (Mytilus galloprovincialis) and clams (Venerupis decussata) from Ingril, a French Mediterranean lagoon
Provedor de dados: 5
Autores: Hess, Philipp; Abadie, Eric; Herve, Fabienne; Berteaux, Tom; Sechet, Veronique; Araoz, Romulo; Molgo, Jordi; Zakarian, Armen; Sibat, Manoella; Rundberget, Thomas; Miles, Christopher O.; Amzil, Zouher.
Following a review of official control data on shellfish in France, Ingril Lagoon had been identified as a site where positive mouse bioassays for lipophilic toxins had been repeatedly observed. These unexplained mouse bioassays, also called atypical toxicity, coincided with an absence of regulated toxins and rapid death times in mice observed in the assay.  The present study describes pinnatoxin G as the main compound responsible for the toxicity observed using the mouse bioassay for lipophilic toxins. Using a well-characterised standard for pinnatoxin G, LC-MS/MS analysis of mussel samples collected from 2009 to 2012 revealed regular occurrences of pinnatoxin G at levels sufficient to account for the toxicity in the mouse bioassays. Baseline levels of...
Tipo: Text
Palavras-chave: Cyclic imines; Shellfish toxin; Accumulation; Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS); Unexplained mouse toxicity.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00158/26960/25255.pdf

Azaspiracid accumulation, detoxification and biotransformation in blue mussels (Mytilus edulis) experimentally fed Azadinium spinosum
Provedor de dados: 5
Autores: Jauffrais, Thierry; Marcaillou-le Baut, Claire; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Nicolau, Elodie; Tillmann, Urban; Hess, Philipp.
Azadinium spinosum (Elbrächter and Tillmann), a small marine dinoflagellate, has been recently described as a de novo producer of azaspiracid-1 and -2 (AZA1 and -2) diarrhoeic toxins. A culture of A. spinosum was established in our laboratory and optimised for pilot-scale production of this organism, to evaluate and understand AZA1 and -2 accumulation and biotransformation in blue mussels (Mytilus edulis) fed with A. spinosum.  Adult mussels were continuously exposed to A. spinosum over 1 week in 160 L cylindrical conical tanks. Three different diets were tested for contamination: 5000, 10 000 cells mL−1 of A. spinosum and a mixture of 5000 cells mL−1 of A. spinosum with 5000 cells mL−1 of Isochrysis aff. galbana (T-Iso, CCAP 927/14). During the subsequent...
Tipo: Text
Palavras-chave: Azaspiracid; Azadinium spinosum; Marine biotoxins; AZA; Tissue distribution; Histology; Bivalve molluscs; Liquid chromatography coupled to tandem mass spectrometry.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00088/19877/17566.pdf

Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts
Provedor de dados: 5
Autores: Estevez, Pablo; Sibat, Manoella; Leão-martins, José Manuel; Reis Costa, Pedro; Gago-martínez, Ana; Hess, Philipp.
Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the...
Tipo: Text
Palavras-chave: Ciguatoxins; HRMS; Q-TOF; Ciguatera poisoning; C-CTX1; Fragmentation pathways.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00624/73630/73067.pdf

Azaspiracid Toxins: Toxicological Profile
Provedor de dados: 5
Autores: Hess, Philipp; Twiner, Michael J; Kilcoyne, Jane; Sosa, Sylvio.
Azaspiracids (AZAs) are a toxin group that originate from marine dinoflagellates of the genera Azadinium and Amphidoma. After accumulation of these toxins in edible marine organisms and their subsequent consumption, humans develop a gastrointestinal syndrome referred to as azaspiracid shellfish poisoning (AZP). This syndrome is very similar to diarrheic shellfish poisoning (DSP), with main symptoms appearing after a few hours from consumption and including diarrhea, vomiting, and stomach cramps. Due to extensive metabolism in shellfish, more than 30 analogues have been reported to date, and purified compounds for selected analogues have recently been made available for toxicological studies. Currently, only AZA1, AZA2, and AZA3 are regulated in Europe and...
Tipo: Text
Palavras-chave: Azaspiracids; Azaspiracid shellfish poisoning; Harmful algae; Azadinium; Shellfish.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00320/43146/42744.pdf

Toxin and Growth Responses of the Neurotoxic Dinoflagellate Vulcanodinium rugosum to Varying Temperature and Salinity
Provedor de dados: 5
Autores: Abadie, Eric; Muguet, Alexia; Berteaux, Tom; Chomerat, Nicolas; Hess, Philipp; Roque D'Orbcastel, Emmanuelle; Masseret, Estelle; Laabir, Mohamed.
Vulcanodinium rugosum, a recently described species, produces pinnatoxins. The IFR-VRU-01 strain, isolated from a French Mediterranean lagoon in 2010 and identified as the causative dinoflagellate contaminating mussels in the Ingril Lagoon (French Mediterranean) with pinnatoxin-G, was grown in an enriched natural seawater medium. We tested the effect of temperature and salinity on growth, pinnatoxin-G production and chlorophyll a levels of this dinoflagellate. These factors were tested in combinations of five temperatures (15, 20, 25, 30 and 35 °C) and five salinities (20, 25, 30, 35 and 40) at an irradiance of 100 µmol photon m−2 s−1. V. rugosum can grow at temperatures and salinities ranging from 20 °C to 30 °C and 20 to 40, respectively. The optimal...
Tipo: Text
Palavras-chave: Vulcanodinium rugosum; Mediterranean Ingril Lagoon; Toxin production; Growth conditions; Temperature; Salinity.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00333/44429/44095.pdf

COSELMAR. Compréhension des socio-écosystèmes littoraux et marins pour la prévention et la gestion des risques. Bilan scientifique 2013-2017
Provedor de dados: 5
Autores: Pardo, Sophie; Hess, Philipp; Simon, Elodie; Barille, Laurent; Geslin, Emmanuelle; Cognie, Bruno; Martin-jezequel, Véronique; Sechet, Véronique; Herrenknecht, Christine; Baron, Regis; Bourseau, Patrick; Amzil, Zouher; Masse, Anthony; Vandanjon, Laurent; Dumay, Justine; Lebeau, Thierry; Turpin, Vincent; Mondeguer, Florence; Petitgas, Pierre; Trouillet, Brice; Mercier, Denis; Guillotreau, Patrice; Guineberteau, Thierry; Mahevas, Stephanie; Schoefs, Franck; Sophie Pardo; Philipp Hess; Pauline Borgniet; Laurent Barillé; Emmanuelle Geslin; Bruno Cognie; Véronique Martin-Jezequel; Véronique Sechet; Christine Herrenknecht; Régis Baron; Patrick Bourseau; Zouher Amzil; Anthony Massé; Laurent Vandanjon; Justine Dumay; Thierry Lebeau; Vincent Turpin; Florence Mondeguer; Pierre Petitgas; Brice Trouillet.
COSELMAR, a 4-year project financed by the Région des Pays de la Loire at 2.1 M€, officially started on the 7th January 2013. The project is coordinated by Philipp Hess (IFREMER) and Sophie Pardo (Université de Nantes-LEMNA) and falls under the Federation for Research Institut Universitaire Mer et Littoral (IUML, FR CNRS 3473)*. COSELMAR has also been approved by the Scientific Council of the MSH Ange Guépin. COSELMAR is a research project uniting 5 research units of IFREMER and 11 laboratories of the Université de Nantes, along with academic partners, and national and international industries. The aim is to achieve a better understanding of the marine and coastal ecosystems and the associated resources. The project will also provide insights into risk...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00406/51793/52550.pdf

Assessment and management of biotoxin risks in bivalve molluscs
Provedor de dados: 5
Autores: Lawrence, Jim; Loreal, Henri; Toyofuku, Hajime; Hess, Philipp; Iddya, Karunasagar; FAO.
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00085/19588/17212.pdf

Effects of Heating on Proportions of Azaspiracids 1–10 in Mussels (     Mytilus edulis     ) and Identification of Carboxylated Precursors for Azaspiracids 5, 10, 13, and 15
Provedor de dados: 5
Autores: Kilcoyne, Jane; Mccarron, Pearse; Hess, Philipp; Miles, Christopher O.
Azaspiracids (AZAs) are marine biotoxins that induce human illness following the consumption of contaminated shellfish. European Union regulation stipulates that only raw shellfish are tested, yet shellfish are often cooked prior to consumption. Analysis of raw and heat-treated mussels (Mytilus edulis) naturally contaminated with AZAs revealed significant differences (up to 4.6-fold) in AZA1–3 (1–3) and 6 (6) values due to heat-induced chemical conversions. Consistent with previous studies, high levels of 3 and 6 were detected in some samples that were otherwise below the limit of quantitation before heating. Relative to 1, in heat-treated mussels the average (n = 40) levels of 3 (range, 11–502%) and 6 (range, 3–170%) were 62 and 31%, respectively. AZA4...
Tipo: Text
Palavras-chave: Azaspiracid; Decarboxylation; Hydroxylation; Chemical conversion; Heating mass spectrometry; Metabolism.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00303/41433/41005.pdf

Derivation of toxicity equivalency factors for marine biotoxins associated with Bivalve Molluscs
Provedor de dados: 5
Autores: Botana, Luis M.; Hess, Philipp; Munday, Rex; Nathalie, Arnich; Degrasse, Stacey L.; Feeley, Mark; Suzuki, Toshiyuki; Van Den Berg, Martin; Fattori, Vittorio; Gamarro, Esther Garrido; Tritscher, Angelika; Nakagawa, Rei; Karunasagar, Iddya.
Background  Seafood toxins pose an important risk to human health, and maximum levels were imposed by regulatory authorities throughout the world. Several toxin groups are known, each one with many analogues of the major toxin. Regulatory limits are set to ensure that commercially available seafood is not contaminated with unsafe levels. Scope and Approach  The mouse bioassay was used to measure the toxicity in seafood extracts to determine if a sample exceeded regulatory limits. The advantage of this approach was to provide an estimation of the total toxicity in the sample. As instrumental methods of analysis advance and serve as replacements to the mouse bioassay, the challenge is translating individual toxin concentrations into toxicity to determine...
Tipo: Text
Palavras-chave: Marine toxins; Toxicity Equivalency Factors; FAO; WHO; Bivalve; Mollusc.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00358/46960/46874.pdf

Oceans and Human Health: A Rising Tide of Challenges and Opportunities for Europe
Provedor de dados: 5
Autores: Fleming, L.e.; Mcdonough, N.; Austen, M.; Mee, L.; Moore, Michael; Hess, Philipp; Depledge, M.h.; White, M.; Philippart, Katja; Bradbrook, P.; Smalley, A.
The European Marine Board recently published a position paper on linking oceans and human health as a strategic research priority for Europe. With this position paper as a reference, the March 2014 Cornwall Oceans and Human Health Workshop brought together key scientists, policy makers, funders, business, and non governmental organisations from Europe and the US to review the recent interdisciplinary and cutting edge research in oceans and human health specifically the growing evidence of the impacts of oceans and seas on human health and wellbeing (and the effects of humans on the oceans). These impacts are a complex mixture of negative influences (e.g. from climate change and extreme weather to harmful algal blooms and chemical pollution) and beneficial...
Tipo: Text
Palavras-chave: Harmful algal blooms; Microbial pollution; Anthropogenic chemicals; Marine biotechnology; Climate change; Ocean acidification; Fisheries; Aquaculture; Seafood; Blue carbon; Marine energy; Blue gym; Ocean literacy; Risks; Benefits; EU Policy.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00192/30328/29066.pdf

Acute Oral Toxicity of Pinnatoxin G in Mice
Provedor de dados: 5
Autores: Sosa, Silvio; Pelin, Marco; Cavion, Federica; Herve, Fabienne; Hess, Philipp; Tubaro, Aurelia.
Pinnatoxin G (PnTx-G) is a marine cyclic imine toxin produced by the dinoflagellate Vulcanodinium rugosum, frequently detected in edible shellfish from Ingril Lagoon (France). As other pinnatoxins, to date, no human poisonings ascribed to consumption of PnTx-G contaminated seafood have been reported, despite its potent antagonism at nicotinic acetylcholine receptors and its high and fast-acting toxicity after intraperitoneal or oral administration in mice. The hazard characterization of PnTx-G by oral exposure is limited to a single acute toxicity study recording lethality and clinical signs in non-fasted mice treated by gavage or through voluntary food ingestion, which showed differences in PnTx-G toxic potency. Thus, an acute toxicity study was carried...
Tipo: Text
Palavras-chave: Vulcanodinium rugosum; Pinnatoxin G; Dinoflagellates; Harmful algae; Oral toxicity.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00605/71724/70190.pdf

Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia
Provedor de dados: 5
Autores: Longo, Sebastien; Sibat, Manoella; Viallon, Jérôme; Darius, Hélène Taiana; Hess, Philipp; Chinain, Mireille.
Ciguatera poisoning (CP) is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. The toxin production and toxin profiles were explored in four clones of G. polynesiensis originating from different islands in French Polynesia with contrasted CP risk: RIK7 (Mangareva, Gambier), NHA4 (Nuku Hiva, Marquesas), RAI-1 (Raivavae, Australes), and RG92 (Rangiroa, Tuamotu). Productions of CTXs, maitotoxins (MTXs), and gambierone group analogs were examined at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography–tandem mass spectrometry. While none of the strains was found to produce known MTX compounds,...
Tipo: Text
Palavras-chave: Gambierdiscus polynesiensis; Ciguatera; Ciguatoxins; Gambierone; 44-methylgambierone; LC-MS/MS; CBA-N2a; Toxin profiles.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00599/71134/69449.pdf

Toxicity screening of 13 Gambierdiscus strains using neuro-2a and erythrocyte lysis bioassays
Provedor de dados: 5
Autores: Pisapia, Francesco; Holland, William C.; Hardison, D. Ransom; Litaker, R. Wayne; Fraga, Santiago; Nishimura, Tomohiro; Adachi, Masao; Lam Nguyen-ngoc,; Sechet, Veronique; Amzil, Zouher; Herrenknecht, Christine; Hess, Philipp.
Species in the epi-benthic dinoflagellate genus Gambierdiscus produce ciguatoxins (CTXs) and maitotoxins (MTXs), which are among the most potent marine toxins known. Consumption of fish contaminated with sufficient quantities of CTXs causes Ciguatera Fish Poisoning (CFP), the largest cause of non-bacterial food poisoning worldwide. Maitotoxins, which can be found in the digestive system of fish, could also contribute to CFP if such tissues are consumed. Recently, an increasing number of Gambierdiscus species have been identified; yet, little is known about the variation in toxicity among Gambierdiscus strains or species.  This study is the first assessment of relative CTX- and MTX-toxicity of Gambierdiscus species from areas as widespread as the...
Tipo: Text
Palavras-chave: Ciguatera fish poisoning; Gambierdiscus; Ciguatoxins; Maitotoxins; Neuro-2a assay; Erythrocyte lysis assay.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00374/48552/49411.pdf

Experimental evidence of dietary ciguatoxin accumulation in an herbivorous coral reef fish
Provedor de dados: 5
Autores: Clausing, Rachel J.; Losen, Barbara; Oberhaensli, Francois R.; Darius, H. Taiana; Sibat, Manoella; Hess, Philipp; Swarzenski, Peter W.; Chinain, Mireille; Bottein, Marie-yasmine Dechraoui.
Ciguatoxins (CTXs) are potent algal toxins that cause widespread ciguatera poisoning and are found ubiquitously in coral reef food webs. Here we developed an environmentally-relevant, experimental model of CTX trophic transfer involving dietary exposure of herbivorous fish to the CTX-producing microalgae Gambierdiscus polynesiensis. Juvenile Naso brevirostris were fed a gel-food embedded with microalgae for 16 weeks (89 cells g.1 fish daily, 0.4 ¦Ìg CTX3C equiv kg.1 fish). CTXs in muscle tissue were detectable after 2 weeks at levels above the threshold for human intoxication (1.2 ¡À 0.2 ¦Ìg CTX3C equiv kg.1). Although tissue CTX concentrations stabilized after 8 weeks (¡«3 ¡À 0.5 ¦Ìg CTX3C equiv kg.1), muscle toxin burden (total ¦Ìg CTX in muscle tissue)...
Tipo: Text
Palavras-chave: Ciguatoxin; Bioaccumulation; Growth dilution; Trophic transfer; Herbivorous fish; Experimental model.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00442/55339/60476.pdf

Metabolomic Profiles of Dinophysis acuminata and Dinophysis acuta Using Non-Targeted High-Resolution Mass Spectrometry: Effect of Nutritional Status and Prey
Provedor de dados: 5
Autores: Garcia-portela, Maria; Reguera, Beatriz; Sibat, Manoella; Altenburger, Andreas; Rodriguez, Francisco; Hess, Philipp.
Photosynthetic species of the genus Dinophysis are obligate mixotrophs with temporary plastids (kleptoplastids) that are acquired from the ciliate Mesodinium rubrum, which feeds on cryptophytes of the Teleaulax-Plagioselmis-Geminigera clade. A metabolomic study of the three-species food chain Dinophysis-Mesodinium-Teleaulax was carried out using mass spectrometric analysis of extracts of batch-cultured cells of each level of that food chain. The main goal was to compare the metabolomic expression of Galician strains of Dinophysis acuminata and D. acuta that were subjected to different feeding regimes (well-fed and prey-limited) and feeding on two Mesodinium (Spanish and Danish) strains. Both Dinophysis species were able to grow while feeding on both...
Tipo: Text
Palavras-chave: Dinophysis physiology; Lipophilic toxins; Non-targeted analysis; Metabolomics.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00439/55077/56508.pdf

Micro-algues toxiques et nuisibles de l’océan mondial. COI Manuels et Guides, 68
Provedor de dados: 5
Autores: Lassus, Patrick; Chomerat, Nicolas; Hess, Philipp; Nezan, Elisabeth.
This book updates available international databases on toxic and harmful species of phytoplankton and micro-phytobenthos. It lists regional occurrences at world-wide scale: localities, cell densities, toxicity levels in contaminated animals, human and animal poisoning, isolation of identified active molecules and toxins, documentary sources. Finally, this book addresses the geographic distributions of phycotoxin-producers from recent data. The objective is to analyze the evolution of toxic and harmful episodes over the past 30 years to identify trends, and to introduce a discussion on the reality of a time-dependent increase in the number of known species and toxins.
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00379/49054/49508.pdf

Characterization of toxin-producing strains of Dinophysis spp. (Dinophyceae) isolated from French coastal waters, with a particular focus on the D. acuminata-complex
Provedor de dados: 5
Autores: Séchet, Veronique; Sibat, Manoella; Bilien, Gwenael; Carpentier, Liliane; Rovillon, Georges-augustin; Raimbault, Virginie; Malo, Florent; Gaillard, Sylvain; Perrière-rumebe, Myriam; Hess, Philipp; Chomérat, Nicolas.
Dinoflagellates of the genus Dinophysis are the most prominent producers of Diarrhetic Shellfish Poisoning (DSP) toxins which have an impact on public health and on marine aquaculture worldwide. In particular, Dinophysis acuminata has been reported as the major DSP agent in Western Europe. Still, its contribution to DSP events in the regions of the English Channel and the Atlantic coast of France, and the role of the others species of the Dinophysis community in these areas are not as clear. In addition, species identification within the D. acuminata complex has proven difficult due to their highly similar morphological features. In the present study, 30 clonal strains of the dominant Dinophysis species have been isolated from French coasts including the...
Tipo: Text
Palavras-chave: Dinophysis spp; . Phylogeny; Taxonomy; Toxin profiles.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00679/79081/81880.pdf

Evidence for the Range Expansion of Ciguatera in French Polynesia: A Revisit of the 2009 Mass-Poisoning Outbreak in Rapa Island (Australes Archipelago)
Provedor de dados: 5
Autores: Chinain, Mireille; Mahana Iti Gatti, Clémence; Ung, André; Cruchet, Philippe; Revel, Taina; Viallon, Jérôme; Sibat, Manoella; Varney, Patrick; Laurent, Victoire; Hess, Philipp; Taiana Darius, Hélène.
Ciguatera poisoning (CP) results from the consumption of seafood contaminated with ciguatoxins (CTXs). This disease is highly prevalent in French Polynesia with several well-identified hotspots. Rapa Island, the southernmost inhabited island in the country, was reportedly free of CP until 2007. This study describes the integrated approach used to investigate the etiology of a fatal mass-poisoning outbreak that occurred in Rapa in 2009. Symptoms reported in patients were evocative of ciguatera. Several Gambierdiscus field samples collected from benthic assemblages tested positive by the receptor binding assay (RBA). Additionally, the toxicity screening of ≈250 fish by RBA indicated ≈78% of fish could contain CTXs. The presence of CTXs in fish was confirmed...
Tipo: Text
Palavras-chave: Ciguatera poisoning; French Polynesia; Gambierdiscus; Ciguatoxins; Epidemiology; Toxicological analyses; Risk management; Climate change.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00662/77362/78933.pdf

The role of Azadinium spinosum (Dinophyceae) in the production of azaspiracid shellfish poisoning in mussels
Provedor de dados: 5
Autores: Salas, Rafael; Tillmann, Urban; John, Uwe; Kilcoyne, Jane; Burson, Amanda; Cantwell, Caoimhe; Hess, Philipp; Jauffrais, Thierry; Silke, Joe.
Azaspiracids (AZAs) are a group of lipophilic polyether compounds first detected in Ireland which have been implicated in shellfish poisoning incidents around Europe. These toxins regularly effect shellfish mariculture operations including protracted closures of shellfish harvesting areas for human consumption. The armoured dinoflagellate Azadinium spinosum Elbrachter et Tillmann gen. et sp. nov. (Dinophyceae) has been described as the de novo azaspiracid toxin producer; nonetheless the link between this organism and AZA toxin accumulation in shellfish has not yet been established. In August 2009, shellfish samples of blue mussel (Mytilus edulis) from the Southwest of Ireland were analysed using liquid chromatography-tandem-mass spectrometry (LC-MS/MS) and...
Tipo: Text
Palavras-chave: Azaspiracids; AZP; AZA toxins; Biodeposits; Dinoflagellates; Feeding experiment; Ireland; LC-MS/MS; Mussels; Phylogeny; Taxonomy.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00049/16049/15836.pdf

Beta-N-Methylamino-L-Alanine: LC-MS/MS Optimization, Screening of Cyanobacterial Strains and Occurrence in Shellfish from Thau, a French Mediterranean Lagoon
Provedor de dados: 5
Autores: Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Brient, Luc; Savar, Veronique; Bardouil, Michele; Hess, Philipp; Amzil, Zouher.
beta-N-methylamino-L-alanine (BMAA) is a neurotoxic non-protein amino acid suggested to be involved in neurodegenerative diseases. It was reported to be produced by cyanobacteria, but also found in edible aquatic organisms, thus raising concern of a widespread human exposure. However, the chemical analysis of BMAA and its isomers are controversial, mainly due to the lack of selectivity of the analytical methods. Using factorial design, we have optimized the chromatographic separation of underivatized analogues by a hydrophilic interaction chromatography coupled to tandem mass spectrometry (HILIC-MS/MS) method. A combination of an effective solid phase extraction (SPE) clean-up, appropriate chromatographic resolution and the use of specific mass spectral...
Tipo: Text
Palavras-chave: Cyanotoxins; BMAA; DAB; AEG; HILIC-MS/MS; Cyanobacteria; Bivalve mollusks; French Mediterranean.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00245/35579/34112.pdf

Algal toxin discovery, management and regulation over the last 25 years
Provedor de dados: 5
Autores: Hess, Philipp.
Tipo: Text
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00450/56180/57728.pdf

Characterization of ovatoxin-h, a new ovatoxin analog, and evaluation of chromatographic columns for ovatoxin analysis and purification
Provedor de dados: 5
Autores: Brissard, Charline; Herve, Fabienne; Sibat, Manoella; Sechet, Veronique; Hess, Philipp; Amzil, Zouher; Herrenknecht, Christine.
The presence of Ostreopsis cf. ovata on the Mediterranean coast represents a serious concern to human health due to production of toxins–putative palytoxin and ovatoxins (ovatoxin-a, -b, -c, -d, -e, -f and -g). However, purified ovatoxins are not widely available and their toxicities are still unknown. In the present study, we report on HR LC-MS/MS analysis of a French Ostreopsis cf. ovata strain (IFR-OST-0.3 V) collected at Villefranche-sur-Mer (France) during a bloom in 2011. Investigation of this strain of Ostreopsis cf. ovata cultivated in our laboratory by ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS) confirmed the production of ovatoxins -a to–e and revealed the presence of a new ovatoxin...
Tipo: Text
Palavras-chave: Ostreopsis cf. ovata; Palytoxin; Ovatoxins; U-HPLC/HR-MSn; Chromatographic separation.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00252/36302/34831.pdf

Identification of 21,22-Dehydroazaspiracids in Mussels (     Mytilus edulis     ) and in Vitro Toxicity of Azaspiracid-26
Provedor de dados: 5
Autores: Kilcoyne, Jane; Mccarron, Pearse; Twiner, Michael J.; Rise, Frode; Hess, Philipp; Wilkins, Alistair L.; Miles, Christopher O.
Azaspiracids (AZAs) are marine biotoxins produced by the genera Azadinium and Amphidoma, pelagic marine dinoflagellates that may accumulate in shellfish resulting in human illness following consumption. The complexity of these toxins has been well documented, with more than 40 structural variants reported that are produced by dinoflagellates, result from metabolism in shellfish, or are extraction artifacts. Approximately 34 μg of a new AZA with MW 823 Da (AZA26 (3)) was isolated from blue mussels (Mytilus edulis), and its structure determined by MS and NMR spectroscopy. AZA26, possibly a bioconversion product of AZA5, lacked the C-20–C-21 diol present in all AZAs reported thus far and had a 21,22-olefin and a keto group at C-23. Toxicological assessment of...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00428/54001/55286.pdf

Metabolomic Analysis of Marine Microalgae Using High Resolution Mass Spectrometry for Taxonomic Comparisons and Screening of Marine Biotoxins
Provedor de dados: 5
Autores: Hess, Philipp; Mondeguer, Florence; Glauner, Thomas; Wust, Bernard; Sibat, Manoella; Zendong, Suzie Zita; Herrenknecht, Christine; Sechet, Veronique.
Out of the circa 5000 microalgae known, around 100 marine species of unicellular algae have been shown to produce several hundred biotoxins worldwide. Such biotoxins may be harmful to man through consumption of fish and shellfish exposed to such algae or through direct contact. Many official methods aimed at the detection of such toxins had traditionally been based on biological assays, due to the lack of reference compounds. Recently, legislation for shellfish safety in Europe introduced targeted MS screening for lipophilic toxins. Due to globalization of trade and climate change, there is a need to develop methods for untargeted screening to fully protect consumer safety, e.g. LC-HRMS. For this purpose we studied the metabolomes of marine microalgae...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00179/29037/27473.pdf

Diversity and Toxicity of the Genus Coolia Meunier in Brazil, and Detection of 44-Methyl Gambierone in Coolia tropicalis
Provedor de dados: 5
Autores: Tibiriçá, Carlos Eduardo Junqueira De Azevedo; Sibat, Manoella; Fernandes, Luciano Felício; Bilien, Gwenael; Chomérat, Nicolas; Hess, Philipp; Mafra, Luiz L.
Coolia is a genus of marine benthic dinoflagellates which is widely distributed in tropical and temperate zones. Toxicity has been reported in selected Coolia species, although the identity of causative compounds is still controversial. In this study, we investigated the taxonomical and toxicological aspects of Coolia species from Brazil. Since light- and electron microscopy-based morphology was not enough to distinguish small-celled species, ITS and LSU D1-D3 phylogenetic analyses were used for species definition. Cultures of Coolia palmyrensis and Coolia santacroce were established from samples collected along the northeastern Brazilian coast, the first record of both species in South Atlantic waters. Cultures of Coolia malayensis and Coolia tropicalis...
Tipo: Text
Palavras-chave: Benthic microalgae; Toxic dinoflagellates; Toxicity assay; Cooliatoxin; 44-methyl gambierone.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00630/74186/73789.pdf

Supporting Information of effects of Heating on AZAs
Provedor de dados: 5
Autores: Kilcoyne, Jane; Mccarron, Pearse; Hess, Philipp; Miles, Christopher O.
This dataset is composed by the supporting information of Kilcoyne Jane, Mccarron Pearse, Hess Philipp, Miles Christopher O. (2015). Effects of Heating on Proportions of Azaspiracids 1–10 in Mussels ( Mytilus edulis ) and Identification of Carboxylated Precursors for Azaspiracids 5, 10, 13, and 15. Journal of Agricultural and Food Chemistry, 63(51), 10980-10987. http://dx.doi.org/10.1021/acs.jafc.5b04609
Tipo: Dataset
Ano: 2015
URL: http://dx.doi.org/10.17882/41845