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Effects of PSMA1 on the differentiation and lipid deposition of bovine preadipocytes
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Li,Sheng; Chen,Chengzhen; Jiang,Hao; Zhang,Jiabao; Zhou,Qian; Gao,Yan; Yuan,Bao; Zhang,Mingjun.
ABSTRACT In this study, our goal was to clarify the role of proteasomal subunit α-1 (PSMA1) in both the differentiation of preadipocytes and the accumulation of lipids in adipocytes. Preadipocytes from healthy one-day-old calves were collected, isolated, and cultured in vitro. The expression pattern of the PSMA1 gene was explored during the differentiation of bovine preadipocytes firstly. Then, the expression of the PSMA1 gene was inhibited by transfection of a chemically synthesized small interfering RNA (siRNA) before differentiation. After induction of differentiation, the mRNA levels of key regulating genes involved in preadipocyte differentiation and the lipid content of mature adipocytes with and without inhibition of PSMA1 were detected by qRT-PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; SiRNA; Transfection; Triglyceride.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100309