Consulta: creatorFacets:"Jollivet, Didier"
Registros recuperados: 15
Data/hora: 10/06/2024 11:14:25

Speciation in the Deep Sea: Multi-Locus Analysis of Divergence and Gene Flow between Two Hybridizing Species of Hydrothermal Vent Mussels
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Faure, Baptiste; Jollivet, Didier; Tanguy, Arnaud; Bonhomme, Francois; Bierne, Nicolas.
Background: Reconstructing the history of divergence and gene flow between closely-related organisms has long been a difficult task of evolutionary genetics. Recently, new approaches based on the coalescence theory have been developed to test the existence of gene flow during the process of divergence. The deep sea is a motivating place to apply these new approaches. Differentiation by adaptation can be driven by the heterogeneity of the hydrothermal environment while populations should not have been strongly perturbed by climatic oscillations, the main cause of geographic isolation at the surface. Methodology/Principal Finding: Samples of DNA sequences were obtained for seven nuclear loci and a mitochondrial locus in order to conduct a multi-locus...
Tipo: Text
Palavras-chave: Mid atlantic ridge; Snake pit area; Population genetics; Drosophila pseudooscura; Bathymodiolus bivalvia; Statistical properties; Subdivided population; Phylogenetic networks; Dna polymorphism; Neutrality tests.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00129/24012/21972.pdf

Characterization and Function of the First Antibiotic Isolated from a Vent Organism: The Extremophile Metazoan Alvinella pompejana
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Tasiemski, Aurelie; Jung, Sascha; Boidin-wichlacz, Celine; Jollivet, Didier; Cuvillier-hot, Virginie; Pradillon, Florence; Vetriani, Costantino; Hecht, Oliver; Soennichsen, Frank D.; Gelhaus, Christoph; Hung, Chien-wen; Tholey, Andreas; Leippe, Matthias; Groetzinger, Joachim; Gaill, Francoise.
The emblematic hydrothermal worm Alvinella pompejana is one of the most thermo tolerant animal known on Earth. It relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. Here, by studying the Pompeii worm, we report on the discovery of the first antibiotic peptide from a deep-sea organism, namely alvinellacin. After purification and peptide sequencing, both the gene and the peptide tertiary structures were elucidated. As epibionts are not cultivated so far and because of lethal decompression effects upon Alvinella sampling, we developed shipboard biological assays to demonstrate that in addition to act in the first line of defense against microbial invasion,...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00219/33052/31517.pdf

Proteome Adaptation to High Temperatures in the Ectothermic Hydrothermal Vent Pompeii Worm
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Jollivet, Didier; Mary, Jean; Gagniere, Nicolas; Tanguy, Arnaud; Fontanillas, Eric; Boutet, Isabelle; Hourdez, Stephane; Segurens, Beatrice; Weissenbach, Jean; Poch, Olivier; Lecompte, Odile.
Taking advantage of the massive genome sequencing effort made on thermophilic prokaryotes, thermal adaptation has been extensively studied by analysing amino acid replacements and codon usage in these unicellular organisms. In most cases, adaptation to thermophily is associated with greater residue hydrophobicity and more charged residues. Both of these characteristics are positively correlated with the optimal growth temperature of prokaryotes. In contrast, little information has been collected on the molecular 'adaptive' strategy of thermophilic eukaryotes. The Pompeii worm A. pompejana, whose transcriptome has recently been sequenced, is currently considered as the most thermotolerant eukaryote on Earth, withstanding the greatest thermal and chemical...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00129/24008/21976.pdf

Revisiting the   Lepetodrilus elevatus   species complex (Vetigastropoda: Lepetodrilidae), using samples from the Galápagos and Guaymas hydrothermal vent systems
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Matabos, Marjolaine; Jollivet, Didier.
The current distribution ranges of vent species result from the complex tectonic history of oceanic ridges. A growing number of DNA barcode studies report the presence of cryptic species across geological discontinuities that offset ridge systems and have gradually helped to draw a more precise picture of the historical migration pathways of vent fauna. We reexamined the phylogeny of species within the Lepetodrilus elevatus complex along the East Pacific Rise (EPR) ridge system in the light of new samples from the Galápagos Rift and the Guaymas Basin. Our analyses of mitochondrial cytochrome c oxidase subunit I gene sequences, coupled with morphological data, highlight the occurrence of a distinct lineage along the Galápagos Rift and offer new insight into...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00479/59048/61792.pdf

Hydrothermal-vent alvinellid polychaete dispersal in the eastern Pacific .1. Influence of vent site distribution, bottom currents, and biological patterns
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Chevaldonne, Pierre; Jollivet, Didier; Vangriesheim, Annick; Desbruyeres, Daniel.
Deep-sea hydrothermal-vent habitats are typically linear, discontinuous, and short-lived. Some of the vent fauna such as the endemic polychaete family Alvinellidae are thought to lack a planktotrophic larval stage and therefore not to broadcast-release their offspring. The genetic evidence points to exchanges on a scale that seems to contradict this type of reproductive pattern. However, the rift valley may topographically rectify the bottom currents, thereby facilitating the dispersal of propagules between active vent sites separated in some cases by 10s of kilometers or more along the ridge axis. A propagule flux model based on a matrix of intersite distances, long-term current-meter data, and information on the biology and ecology of Alvinellidae was...
Tipo: Text
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00337/44780/44597.pdf

Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Holder, Thomas; Basquin, Claire; Ebert, Judith; Randel, Nadine; Jollivet, Didier; Conti, Elena; Jekely, Gaspar; Bono, Fulvia.
Background: Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation.  Results: In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00185/29627/28001.pdf

Sister-species of eastern Pacific hydrothermal vent worms (Ampharetidae, Alvinellidae, Vestimentifera) provide new mitochondrial COI clock calibration
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Chevaldonne, Pierre; Jollivet, Didier; Desbruyeres, Daniel; Lutz, Richard; Vrijenhoek, Robert.
Introduction : The evolutionary age of some of the hydrothermal vent taxa have recently become a highly debated issue. Vestimentiferan worms (= siboglinid polychaetes) in particular, have been claimed to be as old as 430 million years based on the occurrence of fossilized tubes resembling those of currently known Vestimentifera (Little et al., 1997), while other authors, based on molecular data, have claimed they must be younger than 100 million years old (Black et al, 1997; Halanych et al., 1998). One explanation for this apparent discrepancy might be that tubes, such as those found in the Silurian fossil vent communities, did not belong to vestimentiferans, but rather to other tube-dwelling polychaetes. However, it is also possible that the choice of the...
Tipo: Text
Palavras-chave: Allopatric speciation; Polychaetes; Polychaetes; Hydrothermal vent.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-895.pdf

"Bivalvomix" : génomique évolutive des bivalves marins
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bierne, Nicolas; Boudry, Pierre; Lapegue, Sylvie; Bonhomme, Francois; Faure, M; Sauvage, Christopher; Moraga, D; Boutet, Isabelle; David, Elise; Jollivet, Didier; Tanguy, Arnaud; Faure, Baptiste; David, Patrice.
For a long time, population geneticists have had to content themselves with analysing neutral markers to infer selection processes indirectly. The recent improvement of sequencing tools now enables them to analyse the variations of a large number of genes and therefore to spot the genes, or the amino acids, which are under the direct influence of the selection (Yang &amp; Bielawski, 2000). In order to analyse the selection processes acting on the mutations and discriminate the different factors acting on their evolution (genetic selection or drift), it is essential to compare polymorphism data between genomes of the same species/population and divergence data between species/populations (Kimura, 1983). Recently, polymorphism data has become available for a...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-6489.pdf

Worms’ Antimicrobial Peptides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bruno, Renato; Maresca, Marc; Canaan, Stéphane; Cavalier, Jean-françois; Mabrouk, Kamel; Boidin-wichlacz, Céline; Olleik, Hamza; Zeppilli, Daniela; Brodin, Priscille; Massol, François; Jollivet, Didier; Jung, Sasha; Tasiemski, Aurélie.
Antimicrobial peptides (AMPs) are natural antibiotics produced by all living organisms. In metazoans, they act as host defense factors by eliminating microbial pathogens. But they also help to select the colonizing bacterial symbionts while coping with specific environmental challenges. Although many AMPs share common structural characteristics, for example having an overall size between 10–100 amino acids, a net positive charge, a γ-core motif, or a high content of cysteines, they greatly differ in coding sequences as a consequence of multiple parallel evolution in the face of pathogens. The majority of AMPs is specific of certain taxa or even typifying species. This is especially the case of annelids (ringed worms). Even in regions with extreme...
Tipo: Text
Palavras-chave: Antibiotics; Annelids; Nematodes; AMP; Extremophiles.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00511/62307/66556.pdf

Diversification, Evolution and Sub-Functionalization of 70kDa Heat-Shock Proteins in Two Sister Species of Antarctic Krill: Differences in Thermal Habitats, Responses and Implications under Climate Change
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cascella, Kevin; Jollivet, Didier; Papot, Claire; Leger, Nelly; Corre, Erwan; Ravaux, Juliette; Clark, Melody S.; Toullec, Jean-yves.
Background  A comparative thermal tolerance study was undertaken on two sister species of Euphausiids (Antarctic krills) Euphausia superba and Euphausia crystallorophias. Both are essential components of the Southern Ocean ecosystem, but occupy distinct environmental geographical locations with slightly different temperature regimes. They therefore provide a useful model system for the investigation of adaptations to thermal tolerance. Methodology/Principal Finding  Initial CTmax studies showed that E. superba was slightly more thermotolerant than E. crystallorophias. Five Hsp70 mRNAs were characterized from the RNAseq data of both species and subsequent expression kinetics studies revealed notable differences in induction of each of the 5 orthologues...
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00293/40445/39081.pdf

A New Barrier to Dispersal Trapped Old Genetic Clines That Escaped the Easter Microplate Tension Zone of the Pacific Vent Mussels
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Plouviez, Sophie; Faure, Baptiste; Le Guen, Dominique; Lallier, Francois; Bierne, Nicolas; Jollivet, Didier.
Comparative phylogeography of deep-sea hydrothermal vent species has uncovered several genetic breaks between populations inhabiting northern and southern latitudes of the East Pacific Rise. However, the geographic width and position of genetic clines are variable among species. In this report, we further characterize the position and strength of barriers to gene flow between populations of the deep-sea vent mussel Bathymodiolus thermophilus. Eight allozyme loci and DNA sequences of four nuclear genes were added to previously published sequences of the cytochrome c oxidase subunit I gene. Our data confirm the presence of two barriers to gene flow, one located at the Easter Microplate (between 21 degrees 33'S and 31 degrees S) recently described as a hybrid...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00185/29622/28006.pdf

Molecular identification of differentially regulated genes in the hydrothermal-vent species Bathymodiolus thermophilus and Paralvinella pandorae in response to temperature
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boutet, Isabelle; Jollivet, Didier; Shillito, Bruce; Moraga, Dario; Tanguy, Arnaud.
Background: Hydrothermal vents and cold seeps represent oases of life in the deep-sea environment, but are also characterized by challenging physical and chemical conditions. The effect of temperature fluctuations on vent organisms in their habitat has not been well explored, in particular at a molecular level, most gene expression studies being conducted on coastal marine species. In order to better understand the response of hydrothermal organisms to different temperature regimes, differentially expressed genes (obtained by a subtractive suppression hybridization approach) were identified in the mussel Bathymodiolus thermophilus and the annelid Paralvinella pandorae irlandei to characterize the physiological processes involved when animals are subjected...
Tipo: Text
Palavras-chave: Oyster crassostrea gigas; Heat shock protein; Mid atlantic ridge; Cytosolic malate dehydrogenases; Extracellular hemoglobins; Lipid peroxidation; Northeast pacific; Enzyme activities; Arginine kinase; Dual symbiosis.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00129/24014/21970.pdf

Heat-shock response and temperature resistance in the deep-sea vent shrimp Rimicaris exoculata
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ravaux, Juliette; Gaill, Françoise; Le Bris, Nadine; Sarradin, Pierre-marie; Jollivet, Didier; Shillito, Bruce.
The shrimp Rimicaris exoculata swarms around hydrothermal black smoker chimneys at most vent sites along the Mid-Atlantic Ridge. This species maintains close proximity to the hydrothermal fluid, where temperatures can reach 350degreesC and steep thermal and chemical gradients are expected. We performed in vivo experiments in pressurized aquaria to determine the upper thermal limit [critical thermal maximum (CTmax)] of R. exoculata and to investigate some characteristics of the shrimp stress response to heat exposure. These experiments showed that the shrimp does not tolerate sustained exposure to temperatures in the 33-37degreesC range (CTmax). A heat-inducible stress protein belonging to the hsp70 family was identified in R. exoculata, and its synthesis...
Tipo: Text
Palavras-chave: Heat shock; Shrimp; Rimicaris exoculata; Chaperone; IPOCAMP; Caridae; Crustacea; Thermal stress; Hydrothermal vent.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-417.pdf

Videoscopic study of deep-sea hydrothermal vent alvinellid polychaete populations: biomass estimation and behaviour
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Chevaldonne, P; Jollivet, Didier.
Biomass is a poorly documented parameter of the hydrothermal ecosystem, partly due to the difficulties in estimating it. Because the hydrothermal alvinellid polychaete worms mostly live in tubes, and on very irregular surfaces difficult to sample, an original biomass estimation method based on video analysis was proposed and tested on populations of the East Pacific Rise at 13-degrees-N. The area covered by the 3 main alvinellid species was accurately estimated from geometrical calculations in oblique-angle conditions, requiring only 3 object measurements on the monitor screen. The total density of alvinellids was estimated on video by modelling the pattern of worm movements in and out of their tubes. The Richards function was found appropriate to model...
Tipo: Text
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00193/30395/29003.pdf

Temperature resistance of Hesiolyra bergi, a polychaetous annelid living on deep-sea vent smoker walls
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Shillito, Bruce; Jollivet, Didier; Sarradin, Pierre-marie; Rodier, Philippe; Lallier, François; Desbruyeres, Daniel; Gaill, Françoise.
For the first time, in vivo heat-exposure experiments were conducted on the hydrothermal vent polychaete Hesiolyra bergi from the hottest part of the vent biotope. Using a pressurised incubator equipped with video-facilities, we found that H. bergi, which forages around and in the tubes of the thermophilic Alvinella sp., became hyperactive once temperature exceeded 35 degreesC and further lost co-ordination in the 41 to 46 degreesC interval, just before death occurred. Another exposure experiment at 39 degreesC for 3 to 4 h led to 80 % mortality (max) 9 h after heat shock, and 100 % thereafter. In view of the much higher temperatures recorded in this organism's habitat, these results suggest that tolerance to high temperatures (exceeding 40 degreesC) is...
Tipo: Text
Palavras-chave: IPOCAMP; Adaptation to heat; Behaviour; Hydrothermal vents.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/2001/publication-811.pdf