Consulta: creatorFacets:"KAWASE, Yosuke"
Registros recuperados: 3
Data/hora: 10/06/2024 05:47:57

Effect of Zona Incision by Piezo-Micromanipulator (ZIP) on In Vitro Fertilization in 21 Transgenic Mice Lines
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: KAWASE, Yosuke; TACHIBE, Takanori; HANI, Toshio; TATEISHI, Hiromi; JISHAGE, Kou-ichi; SUZUKI, Hiroshi; 鈴木, 宏志.
Zona incision using a piezo-micromanipulator (ZIP) has been demonstrated to be effective for in vitro fertilization (IVF) using cryopreserved C57BL/6 spermatozoa. In this study, ZIP oocytes inseminated with frozen-thawed genetically modified C57BL/6J or FVB mice spermatozoa (21 lines) showed fertilization rates of 22-75% and live fetus rates of 8-49%. In 6 of the lines, the fertilization rates for oocytes compared with ZIP (42-75%) were significantly higher than that of nontreated oocytes (0-50%). Using only 90 oocytes for IVF with ZIP, 5 breeding pairs were produced from cryopreserved genetically modified mice spermatozoa. Our results indicate that application of the ZIP technique is effective for IVF using cryopreserved genetically modified mouse...
Palavras-chave: Frozen-thawed spermatozoa; IVF; ZIP.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3017

A Study on Freeze-Drying as a Method of Preserving Mouse Sperm
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: KAWASE, Yosuke; SUZUKI, Hiroshi; 鈴木, 宏志.
SRD Young Investigator Award
Palavras-chave: Comet assay; Freeze-dried sperm; ICSI; SCSA.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3032

Transportation of Freeze-Dried Mouse Spermatozoa Under Different Preservation Conditions
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: KAWASE, Yosuke; TACHIBE, Takanori; JISHAGE, Kou-ichi; SUZUKI, Hiroshi; 鈴木, 宏志.
Freeze-dried mouse spermatozoa can be used for normal embryonic development after injection into oocytes, thus indicating that freeze-drying is a useful method for the storage and transportation of genetic materials from animals. We recently reported that storage of freeze-dried mouse spermatozoa requires maintenance at temperatures lower than -80 C for long-term preservation and a pressure of 0.37 mbar at primary drying and that these conditions significantly improve the developmental rate to the blastocyst stage. In this study, we examined the influence of transportation and preservation conditions on freeze-dried spermatozoa. Freeze-dried spermatozoa stored for 2 or 2.5 years at 4 or -80 C were transported round trip overland between Shizuoka and...
Palavras-chave: Freeze-dried spermatozoa; Intra cytoplasmic sperm injection (ICSI); Mouse; Transportation.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3027