Consulta: creatorFacets:"Kalil,Susana Juliano"
Registros recuperados: 4
Data/hora: 04/06/2024 14:07:39

Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Antelo,Francine Silva; Costa,Jorge Alberto Vieira; Kalil,Susana Juliano.
C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w) PEG 1500 or 4% (w/w) PEG 8000 (g/gmol) and 23 or 22.5% (w/w) of phosphate resulted a purification factor of 1.6-fold for C-phycocyanin, with total and 57% recovery, respectively. Process conditions were optimized for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-phycocyanin; Purification Factor; Protein; Polyethylene glycol.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100001

Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Manera,Ana Paula; Kamimura,Eliana Setsuko; Brites,Luciana Machado; Kalil,Susana Juliano.
Amyloglucosidase enzyme was produced by Aspergillus niger NRRL 3122 from solid-state fermentation, using deffated rice bran as substrate. The effects of process parameters (pH, temperature) in the equilibrium partition coefficient for the system amyloglucosidase - resin DEAE-cellulose were investigated, aiming at obtaining the optimum conditions for a subsequent purification process. The highest partition coefficients were obtained using 0.025M Tris-HCl buffer, pH 8.0 and 25ºC. The conditions that supplied the highest partition coefficient were specified, the isotherm that better described the amyloglucosidase process of adsorption obtained. It was observed that the adsorption could be well described by Langmuir equation and the values of Qm and Kd...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyloglucosidase; Adsorption isotherm; Kinetics adsorption; Ion exchange resin; Purification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500019

Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Santos,Elisane Odriosolla dos; Michelon,Mariano; Furlong,Eliana Badiale; Burkert,Janaína Fernandes de Medeiros; Kalil,Susana Juliano; Burkert,Carlos André Veiga.
The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Raw glycerol; Single cell protein; Yeast biomass; Yarrowia lipolytica.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200002

RICE PARBOILING WASTEWATER IN THE MAXIMIZATION OF CAROTENOIDS BIOPRODUCTION BY Phaffia rhodozyma
Provedor de dados: Ciência e Agrotecnologia
Autores: Rios,Danielle Alves da Silva; Borba,Thais de Matos de; Kalil,Susana Juliano; Burkert,Janaína Fernandes de Medeiros.
The interest in carotenoid production from natural sources has increased based in their possible use as dyes and their powerful biological antioxidant capacity. This study evaluated the use of rice parboiling wastewater (RPW) as an alternative substrate for the bioproduction of carotenoids using the yeast Phaffia rhodozyma and found it to be promising as the only source of nutrients, reaching a concentration of 0.6 μg mL-1 (259.1 μg g-1) in 48 h. To increase the potential use of this industrial effluent, a study of supplementation was carried out to enhance the production of carotenoids using the methodology of experimental design (a 2IV 6-2 fractional factorial design sequenced by a 24 central composite design). The conditions for maximizing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodye; Submerged cultive; Response surface methodology; Yeast.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000400401