Consulta: creatorFacets:"Keiter, Steffen H."
Registros recuperados: 4
Data/hora: 08/06/2024 19:04:10

Multi- and transgenerational effects following early-life exposure of zebrafish to permethrin and coumarin 47: Impact on growth, fertility, behavior and lipid metabolism
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Blanc, Mélanie; Cormier, Bettie; Hyötyläinen, Tuulia; Krauss, Martin; Scherbak, Nikolai; Cousin, Xavier; Keiter, Steffen H.
Transgenerational effects induced by environmental stressors are a threat to ecosystems and human health. However, there is still limited observation and understanding of the potential of chemicals to influence life outcomes over several generations. In the present study, we investigated the effects of two environmental contaminants, coumarin 47 and permethrin, on exposed zebrafish (F0) and their progeny (F1–F3). Coumarin 47 is commonly found in personal care products and dyes, whereas permethrin is used as a domestic and agricultural pyrethroid insecticide/insect repellent. Zebrafish (F0) were exposed during early development until 28 days post-fertilization and their progeny (F1–F3) were bred unexposed. On one hand, the effects induced by coumarin 47...
Tipo: Text
Palavras-chave: Pyrethroid; Endocrine disrupter; Epigenetic; Transgenerational; Neurotoxic.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00652/76393/77411.pdf

Organic contaminants sorbed to microplastics affect marine medaka fish early life stages development
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Bihanic, Florane; Clérandeau, Christelle; Cormier, Bettie; Crebassa, Jean-claude; Keiter, Steffen H.; Beiras, Ricardo; Morin, Bénédicte; Bégout, Marie-laure; Cousin, Xavier; Cachot, Jerome.
The role of polyethylene microplastics 4–6 μm size (MPs) in the toxicity of environmental compounds to fish early life stages (ELS) was investigated. Marine medaka Oryzias melastigma embryos and larvae were exposed to suspended MPs spiked with three model contaminants: benzo(a)pyrene (MP-BaP), perfluorooctanesulfonic acid (MP-PFOS) and benzophenone-3 (MP-BP3) for 12 days. There was no evidence of MPs ingestion but MPs agglomerated on the surface of the chorion. Fish ELS exposed to virgin MPs did not show toxic effects. Exposure to MP-PFOS decreased embryonic survival and prevented hatching. Larvae exposed to MP-BaP or MP-BP3 exhibited reduced growth, increased developmental anomalies and abnormal behavior. Compared to equivalent waterborne concentrations,...
Tipo: Text
Palavras-chave: Marine medaka; Microplastics; Developmental toxicity; Benzo(a)pyrene; Perfluorooctanesulfonic acid; Benzophenone-3.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00619/73112/74844.pdf

The insecticide permethrin induces transgenerational behavioral changes linked to transcriptomic and epigenetic alterations in zebrafish (Danio rerio)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Blanc, Mélanie; Antczak, Philipp; Cousin, Xavier; Grunau, Christoph; Scherbak, Nikolai; Rüegg, Joëlle; Keiter, Steffen H.
The pyrethroid insecticide permethrin is widely used for agricultural and domestic purposes. Previous data indicated that it acts as a developmental neurotoxicant and can induce transgenerational effects in non-target organisms. However, associated underlying mechanisms remain unclear. The aim of this study was to investigate permethrin-related transgenerational effects in the zebrafish model, and to identify possible molecular mechanisms underlying inheritance. Zebrafish (F0) were exposed to permethrin during early-life (2 h post-fertilization up to 28 days). The F1 and F2 offspring generations were obtained by pairing exposed F0 males and females, and were bred unexposed. Locomotor and anxiety behavior were investigated, together with transcriptomic and...
Tipo: Text
Palavras-chave: Transgenerational epigenetic inheritance; Developmental neurotoxicity; Glutamatergic signaling; DNA methylation; Pesticide.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00685/79661/82425.pdf

An environmentally relevant mixture of polychlorinated biphenyls (PCBs) and polybrominated diphenylethers (PBDEs) disrupts mitochondrial function, lipid metabolism and neurotransmission in the brain of exposed zebrafish and their unexposed F2 offspring
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Blanc, Mélanie; Alfonso, Sebastien; Bégout, Marie-laure; Barrachina, Célia; Hyötyläinen, Tuulia; Keiter, Steffen H.; Cousin, Xavier.
Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants still present in aquatic environments despite their total or partial ban. Previously, we observed that an environmentally realistic mixture of these compounds affects energy balance, growth, and reproduction in exposed zebrafish (F0), and behavior in their unexposed offspring (F1-F4). In the present work, we performed lipidomic and transcriptomic analyses on brains of zebrafish (F0-F2) from exposed and control lineages to identify molecular changes that could explain the observed phenotypes. The use of both technologies highlighted that F0 zebrafish displayed impaired mitochondrial function and lipid metabolism regulation (depletion in...
Tipo: Text
Palavras-chave: Danio rerio; Persistent-organic pollutant; Neurotoxicity; Brain metabolism; RNA-Seq.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00646/75797/76720.pdf