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Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibers
Provedor de dados: 69
Autores: Cheema,Hafiza Masooma Naseer; Bashir,Aftab; Khatoon,Asia; Iqbal,Nadia; Zafar,Yusuf; Malik,Kauser A.
The Calotropis procera seed fibers provide an excellent model system to study the genes involved in fiber elongation, fineness and strength. Expansins constitute one of the important gene families involved in plant cell expansion and other cell wall modification processes. Four homologs of Expansin A gene i.e. CpEXPA1, CpEXPA2, CpEXPA3 and CpEXPA4 were isolated from the cDNA library obtained from fast growing Calotropis procera fibers. These homologs represented typical Expansin A family. Each of them had two conserved domains including GH45 like domain and the putative polysaccharide binding domain. The deduced amino acid sequences of the homologs indicated three conserved motifs: i) eight cysteine residues at N-terminus, ii) four tryptophan residues at...
Tipo: Journal article
Palavras-chave: Calotropis procera; CDNA library; Cotton fibers; EXPANSIN A; Signal peptide; Transcriptome profiling.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600010