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Identification of the B, Q, and native Brazilian biotypes of the Bemisia tabaci species complex using Scar markers
Provedor de dados: 86
Autores: Queiroz,Paulo Roberto; Martins,Erica Soares; Klautau,Nazaré; Lima,Luzia; Praça,Lilian; Monnerat,Rose Gomes.
Abstract: The objective of this work was to develop sequence-characterized amplified region (Scar) markers to identify the B, Q, and native Brazilian biotypes of the sweet potato whitefly [Bemisia tabaci (Hemiptera: Aleyrodidae)]. Random amplified polymorphic DNA (RAPD) amplification products, exclusive to the B and Brazilian biotypes, were selected after the analysis of 12,000 samples, in order to design a specific Scar primer set. The BT-B1 and BT-B3 Scar markers, used to detect the B biotype, produced PCR fragments of 850 and 582 bp, respectively. The BT-BR1 Scar marker, used to identify the Brazilian biotype, produced a PCR fragment of 700 bp. The Scar markers were tested against the Q biotype, and a flowchart was proposed to indicate the decision...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aleyrodidae; Commodities; Field samples; Molecular marker; Sweet potato whitefly.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500555