Consulta: creatorFacets:"Kroon,Erna Geessien"
Registros recuperados: 2
Data/hora: 03/06/2024 17:16:18

Genetic variability of HIV-1 isolates from Minas Gerais, Brazil
Provedor de dados: Revista de Microbiologia
Autores: Proietti,Anna Bárbara de Freitas Carneiro; Barbosa,Edel Figueiredo; Silva,Janaína Guernica; Carvalho,Alex Fiorini de; Kroon,Erna Geessien; Ferreira,Paulo César Peregrino.
We report results of nucleotide sequencing and phylogenetic analysis of the env gene of 11 HIV-1 isolates, in Belo Horizonte, Brazil. Ten isolates belonged to HIV-1 subtype B and one was a probable B/F mosaic. This putative B/F recombinant is similar but not identical in its nucleotide sequence to other B/F mosaics described in Brazil.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV-1; Genome; PCR; AIDS; Hemophiliacs.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200010

Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Jung,Lenice Roteia Cardoso; Bomfim,Maria Rosa Quaresma; Kroon,Erna Geessien; Nunes,Álvaro Cantini.
Leptospires are usually classified by methods based on DNA-DNA hybridization and the conventional cross-agglutination absorption test, which uses polyclonal antibodies against lipopolysaccharides. In this study, the amplification of the rpoB gene, which encodes the beta-subunit of RNA polymerase, was used as an alternative tool to identify Leptospira. DNA extracts from sixty-eight serovars were obtained, and the hypervariable region located between 1990 and 2500-bp in the rpoB gene was amplified by polymerase chain reaction (PCR). The 600-bp amplicons of the rpoB gene were digested with the restriction endonucleases TaqI, Tru1I, Sau3AI and MslI, and the restriction fragments were separated by 6% polyacrylamide gel electrophoresis. Thirty-five fragment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; RpoB gene; RFLP; Serovar; DNA typing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200465