Consulta: creatorFacets:"Lange,Carla Christine"
Registros recuperados: 3
Data/hora: 10/06/2024 16:17:07

Expression profile of genes associated with mastitis in dairy cattle
Provedor de dados: 74
Autores: Fonseca,Isabela; Silva,Priscila Vendramini; Lange,Carla Christine; Guimarães,Marta F.M.; Weller,Mayara Morena Del Cambre Amaral; Sousa,Katiene Régia Silva; Lopes,Paulo Sávio; Guimarães,José Domingos; Guimarães,Simone E.F.
In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p &lt; 0.05). It was also higher in BW Holstein animals with clinical mastitis (p &lt; 0.001), but it was not significant when Gyr cows with and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokine; Gene expression; Immune response; Real-time PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400018

Contagem, isolamento e caracterização de bactérias psicrotróficas contaminantes de leite cru refrigerado
Provedor de dados: 65
Autores: Arcuri,Edna Froeder; Silva,Priscilla Diniz Lima da; Brito,Maria Aparecida Vasconcelos Paiva; Brito,José Renaldi Feitosa; Lange,Carla Christine; Magalhães,Margarida Maria dos Anjos.
Com os objetivos de quantificar, isolar e caracterizar bactérias psicrotróficas contaminantes de leite cru refrigerado, produzido na região da Zona da Mata de Minas Gerais e Sudeste do Rio de Janeiro, foram analisadas amostras de leite coletadas de 20 tanques coletivos e 23 tanques individuais. As contagens de bactérias psicrotróficas nas amostras de leite para os dois tipos de tanques de refrigeração variaram entre 10² e 10(7) Unidades Formadoras de Colônias (UFC) ml-1, porém, um maior número de tanques coletivos apresentou contagens acima de 1 x 10(5) UFC ml-1. Foi verificada a predominância de bactérias psicrotróficas gram-negativas (81,2%), que foram identificadas pelos sistemas API 20E e API 20NE nos gêneros: Aeromonas, Alcaligenes, Acinetobacter,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bactérias psicrotróficas gram-negativas bactérias psicrotróficas gram-positivas; Proteases lípases.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000800025

Accurate identification of atypical Staphylococcus chromogenes plasma-clotting strains causing bovine mastitis
Provedor de dados: 65
Autores: Avellar-Costa,Pedro; Santos,Danielle Cabral dos; Lange,Carla Christine; Laport,Marinella Silva; Brito,Maria Aparecida Vasconcelos Paiva; Rossi,Ciro César; Giambiagi-deMarval,Marcia.
ABSTRACT:  We compared the potential of routine techniques used for the identification of Staphylococcus species, aiming to evaluate their accuracy in the detection of 43 Staphylococcus chromogenes strains isolated from bovine mastitis that, despite being a coagulase-negative species, are able to clot plasma. These strains could be mistakenly suspected to be S. aureus and lead to an unappropriated treatment of the disease. MALDI-TOF, PCR-RFLP of the chaperonine gene groEL, and sequencing of the 16S rRNA and elongation factor Tu gene tuf were employed. Results from the four methods were coincident for only half of the strains because of the low accuracy of the groEL PCR-RFLP (51.2% accuracy). Even though all the sequencing results were identical, the high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus chromogenes; Mastitis; MALDI-TOF; Tuf; GroEL.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600452