Consulta: creatorFacets:"Lapegue, Sylvie"
Registros recuperados: 140
Data/hora: 10/06/2024 14:20:35

A preliminary genetic linkage map for the European flat oyster Ostrea edulis L.
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Harley, Crhis; Boudry, Pierre; Heurtebise, Serge; Beaumont, Andy.
The flat oyster Ostrea edulis is the species endemic to European coasts, both Atlantic and Mediterranean. It has been exploited since Roman times, but its aquaculture production decreased from around 20000 tons in the 1950's to 1500 tons today because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has been undertaking a breeding program to produce families of oysters tolerant to Bonamia. In this context, a further step would be to identify QTLs for resistance to this parasite. Therefore, as a first step a genetic map is now being built. The establishment of a genetic linkage map will provide a foundation for the mapping of QTLs, with the ultimate objective to implement...
Tipo: Text
Palavras-chave: QTLs; Ostrea edulis; Flat oyster; Genetic linkage map; Genetic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3466.pdf

Generation and analysis of a 29,745 unique Expressed Sequence Tags from the Pacific oyster (Crassostrea gigas) assembled into a publicly accessible database: the GigasDatabase
Provedor de dados: 5
Autores: Fleury, Elodie; Huvet, Arnaud; Lelong, Christophe; De Lorgeril, Julien; Boulo, Viviane; Gueguen, Yannick; Bachere, Evelyne; Tanguy, Arnaud; Moraga, Dario; Fabioux, Caroline; Lindeque, Penelope; Shaw, Jenny; Reinhardt, Richard; Prunet, Patrick; Davey, Grace; Lapegue, Sylvie; Sauvage, Christopher; Corporeau, Charlotte; Moal, Jeanne; Gavory, Frederick; Wincker, Patrick; Moreews, Francois; Klopp, Christophe; Mathieu, Michel; Boudry, Pierre; Favrel, Pascal.
Background: Although bivalves are among the most-studied marine organisms because of their ecological role and economic importance, very little information is available on the genome sequences of oyster species. This report documents three large-scale cDNA sequencing projects for the Pacific oyster Crassostrea gigas initiated to provide a large number of expressed sequence tags that were subsequently compiled in a publicly accessible database. This resource allowed for the identification of a large number of transcripts and provides valuable information for ongoing investigations of tissue-specific and stimulus-dependant gene expression patterns. These data are crucial for constructing comprehensive DNA microarrays, identifying single nucleotide...
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6770.pdf

A first genetic linkage map of the European flat oyster, Ostrea edulis : towards the identification of Bonamia resistance QTLs
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Beaumont, Andy.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, the next step is to identify QTLs for resistance to this parasite. A genetic map is therefore being established as a basis for QTL work. The reference family is an F2 family produced by single pair mating of two oysters from the same F1 family. The F1 family was itself produced from a bi-parental cross between a wild oyster and an individual from the...
Tipo: Text
Palavras-chave: Genetic linkage map; Parasite; Genetic; QTLs; Resistance; Bonamia ostreae; Ostrea edulis; Flat oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3464.pdf

Fragmentation des populations naturelles d'Ostrea edulis: une adaptation locale de l'huître plate européenne
Provedor de dados: 5
Autores: Harrang, Estelle; Bierne, Nicolas; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie.
L'huître plate européenne Ostrea edulis a connu une période faste dans l'aquaculture jusqu'à la fin des années 1960, lorsque deux parasites, Marteilia refringens (1969) et Bonamia ostreae (1979), ont successivement décimé les bancs naturels de cette espèce endémique, déjà affaiblis par la sur-exploitation de ses gisements. Historiquement présente sur toute la côte européenne, de la Mer du Nord à la Mer Méditerranée, en passant par l'Océan Atlantique, la Mer Adriatique et la Mer Noire, les bancs naturels de cette espèce sont devenus très localisés, laissant penser à des phénomènes d'adaptation locale de l'huître vis à vis de son environnement biotique et abiotique.  La diversité et la structuration génétique de plusieurs populations de l'espèce, à l'échelle...
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Génétique des populations; Sélection Génétique.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12547/9417.pdf

Les apports de la génétique dans la filière ostréicole française
Provedor de dados: 5
Autores: Lapegue, Sylvie; Renault, Tristan.
The most known and certainly the most important contribution of genetics to the oyster indus try is the development of the triploid cupped oyster. Released for the first time in the 1980s, the triploid oyster has triplets of  chromosomes instead of pairs. Currently, triploid oysters are obtained by crossing diploid and tetraploid oysters. These oysters are characterized by a near lack of milt in summer, which makes them generally popular with consumers, and by a faster growth, allowing a shorter production cycle. In parallel, the exploitation of natural resistance in the diversity of French oysters has been considered to improve the health of farmed populations which is a  major challenge for the sustainability of aquaculture farms. Currently, the...
Tipo: Text
Palavras-chave: Huître creuse; Triploïdie; Sélection; Maladies; Ostréiculture; Cupped oyster; Triploidy; Selection; Disease; Shellfish farming.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00498/60931/64326.pdf

Gonad volume assessment in the oyster Crassostrea gigas: Comparison between a histological method and a Magnetic Resonance Imaging (MRI) method
Provedor de dados: 5
Autores: Flahauw, Emilie; Quellec, Stephane; Davenel, Armel; Degremont, Lionel; Lapegue, Sylvie; Hatt, Philippe-jacques.
Previous studies comparing Crassostrea gigas selected lines with different susceptibility to summer mortality concluded that the higher the reproductive effort the lower the survival. But theywere lacking individual followup of this reproductive effort which would be of great interest to help us to understand the physiology of the oysters facing summermortalities. Themost frequently usedmethod for assessing the volume of the gonad is to measure the area it occupies on histological sections. Gonad measurement by magnetic resonance imaging, in contrast, is non-destructive and therefore makes it possible to assess individual evolution as well as to compare individuals. The present study compared the values of gonad volume by thismethodwith the values of gonad...
Tipo: Text
Palavras-chave: Oyster; Crassostrea gigas; Reproduction; Histology; Magnetic resonance imaging; MRI.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00102/21309/19410.pdf

Bilan des productions expérimentales d'huîtres plates de 2005 à 2010 au Laboratoire de Génétique et Pathologie de La Tremblade
Provedor de dados: 5
Autores: Heurtebise, Serge; Harrang, Estelle; Lallias, Delphine; Lapegue, Sylvie.
L’objectif de ce rapport est de détailler les productions d’huîtres plates européennes, Ostrea edulis, dans le cadre des projets de recherche développés autour de l’amélioration génétique de cette espèce. Nous avons choisi de synthétiser les actions de recherche dans un premier temps puis la production des huîtres et les expérimentations dans un ordre chronologique.
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Production expérimentale; Amélioration génétique.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12421/9211.pdf

Rapid expansion of the invasive oyster Crassostrea gigas at its northern distribution limit in Europe: Naturally dispersed or introduced?
Provedor de dados: 5
Autores: Angles D'Auriac, Marc B.; Rinde, Eli; Norling, Pia; Lapegue, Sylvie; Staalstrom, Andre; Hjermann, Dag O.; Thaulow, Jens.
The Pacific oyster, Crassostrea gigas, was introduced to Europe for aquaculture purposes, and has had a rapid and unforeseen northward expansion in northern Europe. The recent dramatic increase in number of C. gigas populations along the species' northern distribution limit has questioned the efficiency of Skagerrak as a dispersal barrier for transport and survival of larvae. We investigated the genetic connectivity and possible spreading patterns between Pacific oyster populations on the southern Norwegian coast (4 localities) and Swedish and Danish populations by means of DNA microsatellite analysis of adult oysters, and by simulating larvae drift. In the simulations we used a 3D oceanographic model to explore the influence of recent climate change...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00385/49589/50101.pdf

Can survival of European flat oysters following experimental infection with Bonamia ostreae be predicted using QTLs?
Provedor de dados: 5
Autores: Harrang, Estelle; Heurtebise, Serge; Faury, Nicole; Robert, Maeva; Arzul, Isabelle; Lapegue, Sylvie.
The present study identifies quantitative trait loci (QTLs) in response to an experimental infection with the parasite responsible for bonamiosis, Bonamia ostreae, in two segregating families of the European flat oyster, Ostrea edulis. We first constructed a genetic-linkage map for each studied family and improved the existing genetic-linkage map for the European flat oyster with a set of SNP markers. This latter map now combines the best accuracy and the best estimate of the genome coverage available for an oyster species. Secondly, by comparing the QTLs detected in this study with those previously published for O. edulis in similar experimental conditions, we identified several potential QTLs that were identical between the different families, and also...
Tipo: Text
Palavras-chave: Ostrea edulis; Single Nucleotide Polymorphism; Disease resistance; Genetic and QTL mapping; Candidate genes.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00271/38244/36429.pdf

Bilan 2015 du dispositif national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Lupo, Coralie; Osta Amigo, Axel; Fleury, Elodie; Robert, Stephane; Garcia, Celine; Arzul, Isabelle; Baillon, Laury; Bechemin, Christian; Canier, Lydie; Chollet, Bruno; Dechamps, Lucie; Dubreuil, Christine; Faury, Nicole; Francois, Cyrille; Godfrin, Yoann; Lapegue, Sylvie; Morga, Benjamin; Travers, Marie-agnes; Tourbiez, Delphine; Masson, Jean-claude; Verin, Francoise; Cordier, Remy; Gangnery, Aline; Louis, Wilfried; Mary, Charlotte; Normand, Julien; Penot, Julia; Cheve, Julien; Dagault, Francoise; Le Jolivet, Aurore; Le Gal, Dominique; Lebrun, Luc; Bellec, Gwenael; Bouget, Jean-francois; Cochennec-laureau, Nathalie; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Pepin, Jean-francois; Seugnet, Jean-luc; D'Amico, Florence; Maurer, Daniele; Le Gall, Patrik; Mortreux, Serge; Baldi, Yoann; Orsoni, Valerie; Bouchoucha, Marc; Le Roy, Valerian; Pouvreau, Stephane; Queau, Isabelle; Lamoureux, Alice.
Depuis 1992, la surveillance de la santé des mollusques marins du littoral français est assurée par le réseau de Pathologie des Mollusques (Repamo). Ses activités s’inscrivent dans le cadre de la Directive Européenne 2006/88/CE. Depuis son évaluation par la plateforme nationale d’épidémiosurveillance en santé animale en 2012, l’objectif de surveillance est la détection précoce des infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage. L’année 2015 est la première année de transition pour laquelle un début d’évolution des modalités de surveillance de la santé des mollusques marins animées par l’Ifremer a été amorcé. Un dispositif hybride de surveillance a été mis en place, s’appuyant sur...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Mortalité; Maladie.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00324/43486/42945.pdf

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles following its introduction for aquaculture production
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andrew; King, J. W.; Turner, J. R.; Lapegue, Sylvie.
The Pacific oyster, Crassostrea gigas, native to northeast Asia, has been translocated widely for the purpose of aquaculture and is among the most important cultured shellfish. C. gigas has a complex history of introductions in Europe. In France, massive introduction was performed in the early 1970’s, allowing massive spatfall in favourable areas to become the basis of the local oyster industry. In the U.K, hatchery propagation was used to supply local production. Some seed was also exported to Northern Europe were demographically independent populations have been reported in the last decades. In the present study 22 sites were sampled in the British Isles, Denmark, France and Spain in order to assess the genetic diversity and population differentiation...
Tipo: Text
Palavras-chave: Invasion genetics; Pacific oyster; Crassostrea gigas; British Isles.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00167/27814/26007.pdf

Etude transcriptomique, génétique et fonctionnelle de la gamétogenèse chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Bigot, L.; Dubos, M. P.; Dheilly, N.; Favrel, P.; Kellner, K.; Lelong, C.; Martinez, A. S.; Riviere, G.; Santerre, C.; Sourdaine, P.; Degremont, Lionel; Flahauw, Emilie; Hatt, Philippe-jacques; Heurtebise, Serge; Lapegue, Sylvie; Azzouzi, N.; Galibert, F.; Boudry, Pierre; Corporeau, Charlotte; Fabioux, Caroline; Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana.
Le projet GAMETOGENES vise à améliorer les connaissances des bases physiologiques et génétiques de la reproduction et des métabolismes associés chez un mollusque d'importance économique l'huître creuse, Crassostrea gigas. Il a pour objectifs (1) la définition de marqueurs clés de la gamétogenèse, de l'investissement reproducteur et du déterminisme du sexe chez une espèce hermaphrodite à protandrie irrégulière (2) la recherche de QTL de survie aux mortalités estivales ou à l'effort de reproduction dont le succès repose sur l'augmentation de la densité de marqueurs sur les cartes génétiques existantes (SNP en particulier) et sur les liens qui pourront être établis entre ces cartes de liaison et une carte physique (3) le développement d'outils pour valider la...
Tipo: Text
Palavras-chave: Génétique; Huître creuse; Crassostrea gigas; Gamétogenèse; QTL; Marqueur; Physiologie.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00107/21781/19355.pdf

Ostreid herpes virus 1 infection in families of the Pacific oyster, Crassostrea gigas, during a summer mortality outbreak: Differences in viral DNA detection and quantification using real-time PCR
Provedor de dados: 5
Autores: Sauvage, Christopher; Pepin, Jean-francois; Lapegue, Sylvie; Boudry, Pierre; Renault, Tristan.
Ostreid herpes virus 1 (OsHV-1) infections, notably reported in Europe and the USA, are closely associated with significant mortalities of the Pacific oyster, Crassostrea gigas, especially during its early stages of life. In summer 2006, we monitored mortality by strict daily verification of three full-sib families of oysters reared under common conditions. We quantified OsHV-1 using real-time PCR in dead and living individuals during and after a mortality event. Mortality events were severe and brief, but significantly different between tested families (cumulative mortality ranging from 1.2 to 49%). Real-time PCR assays revealed different viral DNA loads in dead individuals from different families (P &lt; 0.001). Moreover, the mean level of infection...
Tipo: Text
Palavras-chave: Viral DNA quantification; Real time PCR; OsHV 1; Mortality; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6265.pdf

Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L. (2003)
Provedor de dados: 5
Autores: Lapegue, Sylvie; Taris, Nicolas; Boudry, Pierre; Langlade, Aime; Bonhomme, Francois.
Le milieu marin est un milieu difficile à appréhender car composé d'un ensemble d'organismes et d'écosystèmes caractérisés par des conditions de fonctionnement tout à fait originales et souvent difficiles d'accès. Ces spécificités peuvent être attribuées à la structure spatiale du milieu marin, qui à bien des égards, diffère du milieu terrestre. En effet si les notions de barrières géographiques sont une réalité concrète pour les organismes terrestres, elles restent rarement identifiées en milieu marin (PALUMBI 1994). Dans un tel environnement, les organismes présentent des cycles de vie souvent mal connus, notamment ceux qui associent phase benthique et phase planctonique. L'étude de la structure génétique des populations, dans un espace aussi ouvert aux...
Tipo: Text
Palavras-chave: Diversité génétique; Génétique; Recrutement; Génotypage; Variabilité génétique; Huître plate; Ostrea edulis.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00044/15478/12857.pdf

Variabilité génétique des stocks d'huîtres dans les Pertuis charentais
Provedor de dados: 5
Autores: Cornette, Florence; Lapegue, Sylvie.
Afin de fiabiliser l'approvisionnement en juvéniles de bivalves dans les Pertuis charentais, une information importante à prendre en compte est l'origine des naissains captés. Ainsi, afin de tenter de déterminer la provenance du naissain capté dans chaque zone des Pertuis charentais, l'objectif de l'action Vargenet « Variabilité génétique des stocks d'huîtres dans les Pertuis charentais» est de déterminer si l'outil génétique peut être envisagé en complément des données de suivi de stocks et de modélisation. Pour cela, il est nécessaire de savoir si les stocks sauvages présents dans ces zones peuvent être différenciés génétiquement. Si c'est le cas, les cohortes de naissain captées pourraient alors être assignées à un stock parental, ce qui permettrait...
Tipo: Text
Palavras-chave: Génétique; Variabilité génétique; Naissain; Stocks sauvages; Huître creuse; Crassostrea gigas; Pertuis Charentais; Charente-Maritime.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00055/16661/14154.pdf

Commentaire de l'Ifremer sur le projet de manuel de diagnostic et de surveillance pour les animaux aquatiques
Provedor de dados: 5
Autores: Garcia, Celine; Lapegue, Sylvie; Renault, Tristan.
Tipo: Text
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00217/32853/80979.pdf

Impact of diuron on aneuploidy and hemocyte parameters in Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Bonnard, Marc; Gagnaire, Beatrice; Renault, Tristan; Lapegue, Sylvie.
Diuron is a substituted urea herbicide used for agricultural and nonagricultural weed control. Its widespread use and relatively slow breakdown led us to analyze its influence on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in Pacific oysters, Crassostrea gigas. Adult oysters were subjected to two diuron concentrations (300 ng L-1 and 3 mu g L-1) for 11 weeks. Significantly higher aneuploidy level was observed in diuron-treated oysters compared with the control. Furthermore, the observed impact on aneuploidy persisted to the next generation as offspring exhibited significantly higher aneuploidy levels when their parents had been exposed to diuron. Significant increases in hemocyte parameters (cell...
Tipo: Text
Palavras-chave: Diuron; Herbicide impact; Cytometry; Hemocyte; Aneuploidy; Crassostrea gigas.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2178.pdf

Mapping QTL for resistance to summer mortality in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Sauvage, Christopher; Heurtebise, Serge; De Koning, D.; Haley, Chris; Boudry, Pierre; Lapegue, Sylvie.
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). Such selective breeding would strongly benefit from the identification of markers associated to the resistance to allow the development of markers assisted selection. Recently, a set of a new kinds of markers (SNPs) have been developed in the Pacific oyster. These markers were identified and characterized in several genes involved in the immune response and energetic pathways (Sauvage, 2007). In this context, the mapping of SNPs, of 16 new EST-SSR based markers and of...
Tipo: Text
Palavras-chave: Mortality; Genetic; Crassostrea gigas; Pacific oyster; Mapping QTL.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3504.pdf

PCR survey of 50 introns in animals: Cross-amplification of homologous EPIC loci in eight non-bilaterian, protostome and deuterostome phyla
Provedor de dados: 5
Autores: Gerard, K.; Guilloton, E.; Arnaud-haond, Sophie; Aurelle, D.; Bastrop, R.; Chevaldonne, P.; Derycke, S.; Hanel, R.; Lapegue, Sylvie; Lejeusne, C.; Mousset, Sylvain; Ramsak, A.; Remerie, T.; Viard, Frederique; Feral, Jean-pierre; Chenuil, A.
Exon Primed Intron Crossing (EPIC) markers providemolecular tools that are susceptible to be variable within specieswhile remaining amplifiable by PCR using potentially universal primers. In this studywe tested the possibility of obtaining PCR products from 50 EPIC markers on 23 species belonging to seven different phyla (Porifera, Cnidaria, Arthropoda, Nematoda, Mollusca, Annelida, Echinodermata) using 70 new primer pairs. A previous study had identified and tested those loci in a dozen species, including another phylum, Urochordata (Chenuil et al., 2010). Results were contrasted among species. The best results were achieved with the oyster (Mollusca) where 28 loci provided amplicons susceptible to contain an intron according to their size. This was...
Tipo: Text
Palavras-chave: Universal primers; Alternative barcoding; Non-model species; Genetic marker; Intron.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00166/27775/25969.pdf

Bonamia ostreae-induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf

Single Nucleotide polymorphisms and their relationship to codon usage bias in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Sauvage, Christopher; Bierne, N; Lapegue, Sylvie; Boudry, Pierre.
DNA sequence polymorphism and codon usage bias were investigated in a set of 41 nuclear loci in the Pacific oyster Crassostrea gigas. Our results revealed a very high level of DNA polymorphism in oysters, in the order of magnitude of the highest levels reported in animals to date. A total of 290 single nucleotide polymorphisms (SNPs) were detected, 76 of which being localised in exons and 214 in non-coding regions. Average density of SNPs was estimated to be one SNP every 60 bp in coding regions and one every 40 bp in non-coding regions. Non-synonymous substitutions contributed substantially to the polymorphism observed in coding regions. The non-synonymous to silent diversity ratio was 0.16 on average, which is fairly higher to the ratio reported in other...
Tipo: Text
Palavras-chave: Crassostrea gigas; Codon bias; Genetic diversity; SNP.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3417.pdf

Variabilité génétique de la réponse aux organismes pathogènes : un outil pour améliorer la santé des mollusques et poissons d'élevage
Provedor de dados: 5
Autores: Quillet, Edwige; Boudry, Pierre; Lapegue, Sylvie.
Controlling health of livestock has become of considerable importance to the aquaculture industry. Selective breeding of animals with increased resistance to pathogens is likely to complete efficiently the use of drugs, vaccines or prophylactic approaches that may be difficult to implement. A review of the scientific literature shows that the prospect of selecting for increased resistance is quite hopeful in both molluscs and fish. Yet, selecting for disease resistance is hampered by the difficulty to measure the resistance of individuals. Prospects for the development of «markers» that would predict the genetic merit of individuals without pathogen challenge are presented. The breeding schemes that may be implemented for fish and molluscs in the French...
Tipo: Text
Palavras-chave: Marqueurs phénotypiques; Marqueurs génétiques; Poissons; Mollusques; Agents pathogènes; Variabilité génétique.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2979.pdf

Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens
Provedor de dados: 5
Autores: Renault, Tristan; Tchaleu, Gwenaelle; Faury, Nicole; Moreau, Pierrick; Segarra, Amelie; Barbosa-solomieu, Valerie; Lapegue, Sylvie.
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have been reported among C. gigas in Europe in relation to the detection of a variant of OsHV-1, called μVar. Since 2009, this variant has been mainly detected in France. These results raise questions about the emergence and the virulence of this variant. The search for association between specific virus genetic markers and clinical symptoms is of great interest and the characterization of the genetic variability of OsHV-1 specimens is an area of growing interest....
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00172/28306/26582.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas - Contrat Région Poitou-Charentes 2000-2006. Convention 2003
Provedor de dados: 5
Autores: Bouilly, Karine; Bonnard, Marc; Grouhel, Stephanie; Lapegue, Sylvie.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules présentant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Or, une corrélation négative entre l’aneuploïdie somatique et le taux de croissance a été décrite à de nombreuses reprises (Thiriot-Quiévreux et al., 1988, 1992 ; Leitão et al., 2001a ; Zouros et al., 1996). Bien que les causes environnementale et /ou génétique de l’aneuploïdie restent encore largement inconnues, des études récentes ont permis de poser l’hypothèse d’une base génétique large dans la détermination de ce caractère (Leitão et al., 2001b). De plus, un effet toxique direct de l’atrazine (herbicide couramment utilisé dans la région) sur le génome d’une...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00044/15476/12855.pdf

Genome mapping in shellfish aquaculture species
Provedor de dados: 5
Autores: Lapegue, Sylvie.
The development of fish and shellfish farming is facing several challenges linked to the infancy of  the industry: i.e. domestication, genetic improvement of disease resistance, genetic improvement of feed efficiency to fish meal and fish oil substitution by vegetal products, development of  adapted vaccines, supply of fry or spat at high quality and high robustness, adaptation to environmental change, production of healthy, high quality consumer products and limitation of environmental impact of the activity and escapees. Over recent years, the EU and individual countries have supported the development of genomic tools for some aquaculture species (e.g. rainbow trout, sea bass, sea bream, turbot pacific oyster,  Atlantic salmon). New perspectives arise...
Tipo: Text
Palavras-chave: Mussels; Oysters; Domestication; Genome mapping; Genetic.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3819.pdf

Is the Portuguese oyster Crassostrea angulata in Southern Europe endangered by the expansion of the Pacific oyster C. gigas?
Provedor de dados: 5
Autores: Batista, Frederico; Boudry, Pierre; Lapegue, Sylvie; Heurtebise, Serge; Monteiro, C. C.
The Portuguese oyster, Crassostrea angulata, was introduced from Portugal to the French Atlantic Coast in the 1860s. C. angulata quickly settled and expanded and leaded to the development of a new aquacultural industry in France. In the late 1960s, mortality associated with the detection of an iridovirus, led to the wipe out of C. angulata from French Atlantic waters and to the massive introduction of C. gigas to sustain production. In Southern Europe, similar symptoms were also observed in natural stocks of C. angulata from Sado River (Portugal) and from the area of Cadiz (Spain). Nowadays, only very few «pure» populations of C. angulata remain in southern Europe. These populations are potentially endangered by the current expansion of C. gigas...
Tipo: Text
Palavras-chave: Selection; Hybridization; Settlement pattern; Population genetic; Genetic; Oysters; Crassostrea angulata; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3310.pdf

Impact of atrazine on somatic aneuploidy in cupped oysters, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Chaves, R.; Guedes Pinto, H; Boudry, Pierre; Lapegue, Sylvie.
In the Pacific oyster, Crassostrea gigas hypodiploid aneuploid cells with 2n=19, 18 or 17 chromosomes have regularly been reported. There is a negative correlation between somatic aneuploidy and growth rate in oysters (Leitão et al., 2001a) as well as evidence for a genetic basis (Leitão et al., 2001b). Furthermore, non-random chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique (Leitão et al., 2001c). This poster summarises different studies used to investigate the effects of the herbicide atrazine on the level of aneuploidy in this species
Tipo: Text
Palavras-chave: Genetic; Aneuploidy; Toxicology; Impact; Atrazine; Crassostrea gigas; Pacific oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3489.pdf

Genetic diversity of the European oyster (Ostrea edulis L.) in Nova Scotia: Comparison with other parts of Canada, Maine and Europe and implications for broodstock management
Provedor de dados: 5
Autores: Vercaemer, Bénédikte; Spence, Koren R.; Herbinger, Christophe; Lapegue, Sylvie; Kenchington, Ellen L.
The European oyster (Ostrea edulis) was introduced to the Nova Scotia aquaculture industry 30 years ago using stocks imported from naturalized populations in Maine whose ancestors originated in the Netherlands. This study used 5 microsatellites to assess the level of genetic diversity in several hatchery stocks and naturalized populations from Nova Scotia, New Brunswick, British Columbia and Maine. Some genetic erosion was shown to have occurred in the Maritimes populations, with the largest loss of alleles being experienced by the hatchery stocks. In spite of this loss, genetic diversity and heterozygosity in the Maritimes populations are still relatively high. Relationships within and between the populations and the existence of kin groups within the...
Tipo: Text
Palavras-chave: Genetic diversity; Maine; Microsatellites; Flat oyster; European oyster; Ostrea edulis.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2121.pdf

A first linkage map for the European flat oyster, Ostrea edulis towards the identification of Bonamia resistance QTLs
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, a genetic map was therefore established as a basis for Quantitative Trait Loci (QTLs) analysis. The reference family was an F2 family coming from a first bi-parental cross between a wild oyster and an individual from the fifth generation inbred line of the Bonamia tolerance selection program. This inbred line had shown no mortality when reared in...
Tipo: Text
Palavras-chave: Parasite; Bonamia ostreae; Bonamiosis; QTL; Genetic; Ostrea edulis; Flat oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3459.pdf

Small effective number of parents (N-b) inferred for a naturally spawned cohort of juvenile European flat oysters Ostrea edulis
Provedor de dados: 5
Autores: Hedgecock, Denis; Launey, Sophie; Pudovkin, A. I.; Naciri, Yamama; Lapegue, Sylvie; Bonhomme, Francois.
The great fecundity and very high larval mortality of most marine invertebrates and fish make possible substantial variance in the number of offspring contributed by adults to subsequent generations. The reproductive success of such organisms may thus resemble a sweepstakes lottery, in which a minority of progenitors succeeds in replacing an entire population, while the majority fails to procreate. One specific prediction of this hypothesis, that genetic diversity of newly settled cohorts should be less than that of the adult population, is tested in the present study. Microsatellite DNA markers were examined in naturally spawned juvenile European flat oysters Ostrea edulis (L.), collected over a 12-day period in 1993 from the western Mediterranean Sea,...
Tipo: Text
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2616.pdf

Inventaire, diagnostic écologique et restauration des principaux bancs d’huitres plates en Bretagne : le projet FOREVER
Provedor de dados: 5
Autores: Pouvreau, Stephane; Cochet, Hélène; Fabien, Aurélie; Arzul, Isabelle; Lapegue, Sylvie; Gachelin, Sonia; Salaun, Benoit.
L’huitre plate, la seule huitre native des côtes européennes, est une espèce emblématique de Bretagne. Convoitée depuis l'Antiquité, sur-pêchée au 19ème siècle, fleuron de l'ostréiculture au 20ème, elle est désormais menacée de disparition à l'aube du 21ème. Sous l'impulsion de la commission OSPAR mais aussi dans un contexte grandissant de crise écologique et d'érosion de la biodiversité imposant une nécessaire conservation et/ou restauration de nos patrimoines naturels, une alliance européenne réunissant biologistes et gestionnaires a été créée en novembre 2017 à Berlin sous l'égide de l'Agence Allemande de Conservation de la Nature (BFN) avec un objectif commun: favoriser le retour de l'huitre plate sur les côtes européennes. Cette alliance pour la...
Tipo: Text
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00683/79506/82154.pdf

Niveau de ploïdie des huîtres des bassins de captage de Marennes-Oléron et Arcachon
Provedor de dados: 5
Autores: Cornette, Florence; Grouhel, Stephanie; Sharbel, Tim; Boudry, Pierre; Goulletquer, Philippe; Lapegue, Sylvie.
La mise en place du réseau « biovigilance » résulte des recommandations formulées dans le cadre de l’expertise indépendante demandée par le Comité Scientifique du Ministère de l’Agriculture et de la Pêche concernant « l’effet d’un flux éventuel d’huîtres tétraploïdes dans les zones conchylicoles ». Il avait été alors préconisé de réaliser « une biovigilance légère, avec mesure régulière du taux d’huîtres tétraploïdes dans les bassins conchylicoles ». Le sous-projet « Réseau biovigilance » s’inscrit dans le projet Ifremer « Typologie et surveillance des systèmes de productions aquacoles » du programme de recherches «Durabilité des systèmes de production » du thème « Surveillance et optimisation des productions aquacoles ». Ce réseau a pour objectif d’être...
Tipo: Text
Palavras-chave: Reproduction; Captage; Arcachon; Marennes Oléron; Génétique; Crassostrea gigas; Ploïdie; Huîtres.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00043/15445/12815.pdf

Synthèse PERLE. Programme d’Expérimentation et de recherche sur L’huître plate ostrea Edulis
Provedor de dados: 5
Autores: Hussenot, Mathieu; Pouvreau, Stephane; Duchene, Julie; Freulon, Henry; Arzul, Isabelle; Lapegue, Sylvie.
En complément du rapport final (Anonyme (2014). PERLE. Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis. Rapport final de l'ensemble du projet 2011-2014. http://archimer.ifremer.fr/doc/00248/35932/), ce document présente une synthèse des principaux résultats du programme PERLE et ouvre de multiples perspectives pour les années à venir.
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00249/36060/34610.pdf

Variance of reproductive success in the European flat oyster Ostrea edulis assessed by microsatellite-based parentage analyses in a natural population and in hatchery
Provedor de dados: 5
Autores: Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie.
he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,...
Tipo: Text
Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf

Study of atrazine effects on Pacific oyster, Crassostrea gigas, haemocytes
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Bouilly, Karine; Lapegue, Sylvie; Thomas-guyon, H.
Shellfish farming is an important economic activity around the world. This activity often takes place in areas subjected to various recurring pollutions. The recrudescent use of herbicides in agriculture including atrazine implies pollutant transfer towards aquatic environment in estuarine areas. Harmful effects of such substances on animals in marine environment, particularly on cultured bivalves, are poorly documented. Bivalve molluscs such as mussels and oysters have been postulated as ideal indicator organisms because of their way of life. They filter large volumes of seawater and may therefore accumulate and concentrate eontaJ.11inants within their tissues. Moreover, development of techniques allowing effect analysis of such compounds on bivalve...
Tipo: Text
Palavras-chave: Crassostrea gigas; Haemocytes; Flow cytometry; Atrazine; Cellular activities; Toxicity.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-2827.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas - Contrat de Plan Région Poitou-Charentes 2000-2006. Convention 2000
Provedor de dados: 5
Autores: Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00043/15474/12853.pdf

Trans-Atlantic distribution of a mangrove oyster species revealed by 16S mtDNA and karyological analyses
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Heurtebise, Serge; Garcia, P; Thiriot Quievreux, C; Boudry, Pierre.
Three species of man.-rove oysters, Crassostrea rhizophorae, C. brasiliana, and C. gasar, have been described along the Atlantic shores of South America and Africa. Because the distribution of these molluscs is of great biological and commercial interest, their taxonomy and distribution deserve further clarification. Therefore, 15 populations were sampled from both continents. Their 16S mitochondrial polymorphism was studied by sequencing and PCR-RFLP analysis. Two haplotypes were identified. Haplotype a was the only one observed in Africa, but it was also observed in South America together with haplotype b. Because C. gasar is the only mangrove oyster identified on the west coast of Africa, haplotype a was attributed to this species, which has thus been...
Tipo: Text
Palavras-chave: Mangrove oyster; Crassostrea gasar; Karyotype; Phylogeography; 16S mitochondrial DNA.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2790.pdf

A high load of non-neutral amino-acid polymorphisms explains high protein diversity despite moderate effective population size in a marine bivalve with sweepstakes reproduction
Provedor de dados: 5
Autores: Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas.
Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the...
Tipo: Text
Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf

Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Leitao, Alexandra; Chaves, Raquel; Guedes Pinto, Henrique; Boudry, Pierre; Lapegue, Sylvie.
Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pacific oyster; Restriction enzyme digestion chromosome banding; Atrazine; Aneuploidy; Crassostrea gigas; Huître creuse; Marquage chromosomique par digestion d'enzymes de restriction; Atrazine; Aneuploïdie.
Ano: 2005
URL: https://archimer.ifremer.fr/doc/2005/publication-654.pdf

QTL for resistance to summer mortality and OsHV-1 load in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Sauvage, Christopher; Boudry, Pierre; De Koning, D. -j.; Haley, C. S.; Heurtebise, Serge; Lapegue, Sylvie.
P&gt;Summer mortality is a phenomenon severely affecting the aquaculture production of the Pacific oyster (Crassostrea gigas). Although its causal factors are complex, resistance to mortality has been described as a highly heritable trait, and several pathogens including the virus Ostreid Herpes virus type 1 (OsHV-1) have been associated with this phenomenon. A QTL analysis for survival of summer mortality and OsHV-1 load, estimated using real-time PCR, was performed using five F-2 full-sib families resulting from a divergent selection experiment for resistance to summer mortality. A consensus linkage map was built using 29 SNPs and 51 microsatellite markers. Five significant QTL were identified and assigned to linkage groups V, VI, VII and IX. Analysis of...
Tipo: Text
Palavras-chave: Crassostrea gigas; Herpes virus; Pacific oyster; QTL mapping; Summer mortality.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00011/12241/9063.pdf

Impact de la dissémination accidentelle d'huîtres tétraploïdes : potentiel de dispersion et succès reproducteur
Provedor de dados: 5
Autores: Lapegue, Sylvie; Heurtebise, Serge; Cornette, Florence; Brizard, Raphael; Sharbel, Tim; Boudry, Pierre.
L'ostréiculture française réalise une production très largement extensive, basée sur le recrutement de juvéniles dans le milieu naturel et la production sur des sites côtiers. Ainsi, l’huître est l’une des rares espèces cultivées à être encore considéré par le consommateur comme un produit alimentaire totalement « naturel ». L'utilisation de la polyploïdisation a permis d'ouvrir une nouvelle voie dans cette filière, en permettant d'obtenir des animaux à croissance plus rapide, plus homogènes et stériles. Cette dernière caractéristique est un gage de qualité gustative et permet aussi de positionner ce produit en priorité sur les mois en "r" traditionnellement bannis à cause de la présence de laitance. Le but principal de la triploïdisation a été atteint:...
Tipo: Text
Palavras-chave: Génétique; Triploïdisation; Tétraploïde; Impact; Environnement; Bivalvia; Crassostrea gigas.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00043/15460/12835.pdf

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andy; King, Jonathan W.; Turner, John R.; Lapegue, Sylvie.
The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and...
Tipo: Text
Palavras-chave: Invasion genetics; Marine mollusc; Microsatellites; MtDNA; Pacific oyster; Crassostrea gigas.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00268/37973/36052.pdf

Episodes de mortalité massive de moules bleues observés en 2014 dans les Pertuis charentais
Provedor de dados: 5
Autores: Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan.
Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen
Tipo: Text
Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf

Rapport d'activités 2014 de l'Unité SG2M. Santé, Génétique et Microbiologie des mollusques
Provedor de dados: 5
Autores: Renault, Tristan; Lapegue, Sylvie; Le Guyader, Soizick; Hatt, Philippe-jacques; Rivet, Florence.
L’environnement marin est un système dynamique soumis à une forte variabilité d’origines naturelle et anthropique. Les pressions d’origine anthropique sont diverses (surexploitations des ressources vivantes, rejets de contaminants, aménagements, changements globaux) et ont fortement augmenté aux cours des dernières décennies, générant des évolutions importantes dans les écosystèmes marins et dans les usages qui en dépendent, en particulier en zone côtière. L’amélioration du bon état écologique et des services rendus par les écosystèmes marins pose des questions complexes en termes de fonctionnalité et de biodiversité, de durabilité de l’exploitation des ressources vivantes, des politiques publiques et des interactions entre usages. Ces questions entrent...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00283/39455/37921.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea : hybridation et conservatoire de souches. Contrat Région Poitou-Charentes 1999 - Convention 99 RPC-A-203 "Génétique"
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Dans le cadre du plan Etat-Région 1994-1998, un programme de conservatoire de souches a été initié au Laboratoire IFREMER de La Tremblade. Ce conservatoire a pour objectif d'acclimater différentes espèces ou souches d'huîtres creuses (genres Crassostrea et Saccostrea) afin d'acquérir des connaissances sur ces espèces et d'évaluer l'intérêt qu'elles pourraient présenter pour l'ostréiculture locale. L'importation d'espèces étrangères sur les côtes françaises est désormais strictement réglementée: la dissémination d'une nouvelle espèce peut se révéler néfaste d'un point de vue écologique et nuire aux productions existantes. De plus, ces huîtres peuvent être porteuses de pathogènes qui, s'il venaient à se répandre dans le milieu, pourraient toucher les stocks...
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Croisement; Crassostrea gigas; Crassostrea ariakensis; Crassostrea sikamea; Hybridation.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00044/15514/12901.pdf

Compte rendu des travaux d’accompagnement de l’opération Resur 2 menée par le Comité Régional Conchylicole d’Arcachon
Provedor de dados: 5
Autores: Gouillieux, Benoit; Plus, Martin; Auby, Isabelle; Tchaleu, Gwenaelle; Lapegue, Sylvie.
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14386/11678.pdf

Hybridation naturelle entre les deux sous-espèces d'huîtres creuses Crassostrea gigas et Crassostrea angulata au sud de l'Europe
Provedor de dados: 5
Autores: Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Boudry, Pierre.
Crassostrea angulata and Crassostrea gigas, two cupped oysters of Asian origin (O'Foighil et al, 1998), have been introduced successively in Europe during the XVlth and the XXth century (Grizel et Héral, 1991). The present populations of C. angulata appear to be limited to the south of Spain and Portugal, whereas the distribution of C. gigas in Europe is rising because of the development of oyster farming. The distinction between the two subspecies in based on a RFLP marker of the mitochondrial genome (COI) which shows a strong genetic differentiation between the two subspecies populations (Boudry et al., 1998). On the contrary, the nuclear markers (microsatellites) showed a smaller genetic differentiation between them (Huvet et al, under press). The...
Tipo: Text
Palavras-chave: Microsatellite; Hybridation; Crassostrea angulata; Crassostrea gigas; Huîtres; Génétique.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3282.pdf

Génomique de l'adaptation de l'huître creuse du Pacifique, Crassostrea gigas, dans le cadre de son expansion géographique
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).  Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Pacific oyster; Invasive; AFLP; Genome scan; Crassostrea gigas.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4542.pdf

PERLE - Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis - Rapport final des sous-programmes 2 et 3
Provedor de dados: 5
Autores: Arzul, Isabelle; Baillon, Laury; Boudry, Pierre; Bouget, Jean-francois; Chollet, Bruno; Cochet, Helene; Couraleau, Yann; Dubreuil, Christine; Duchene, Julie; Dutartre, Julie; Garcia, Celine; Heloury, Purotu; Heurtebise, Serge; Huchet, Eve; Hussenot, Mathieu; Langlade, Aime; Ollier, Simon; Omnes, Emmanuelle; Pouvreau, Stephane; Roby, Charlotte; Serpin, Delphine; Viard, Frederique; Wacrenier, Candice; Lapegue, Sylvie.
L’huître plate (Ostrea edulis) est une espèce endémique des côtes européennes, elle est présente depuis des millions d’années. Son aire de répartition est très vaste allant de la Norvège jusqu’au Maroc. Elle est également présente en mer Méditerranée, en mer Noire, mais également au Canada, aux Etats-Unis ou encore en Afrique du Sud, à la suite d'introductions pour des raisons aquacoles. En France, à partir de la fin des années 70, de fortes mortalités ont été constatées sur les bancs naturels et en élevage, faisant rapidement chuter la production de 20 000 tonnes à 2 000 tonnes. Ces mortalités ont été attribuées à deux maladies parasitaires : la marteiliose (due au parasite Marteilia refringens) et la bonamiose (due au parasite Bonamia ostreae). Trente...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00247/35815/34328.pdf

Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Sauvage, Christopher; Boudry, Pierre; Lapegue, Sylvie.
We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-OlEron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.
Tipo: Text
Palavras-chave: Pacific oyster; Null alleles; EST SSR markers; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6419.pdf

Establishment of a genetic map in the European flat oyster Ostrea edulis L.
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Beaumont, Andy.
The flat oyster Ostrea edulis is the species endemic from European coasts, Atlantic and Mediterranean. It has been exploited since Roman times in Europe. But its production decreased from around 20000 tons in the 1950ies to 1500 tons nowadays because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer initiated a program of selection to produce families of oysters tolerant to Bonamia. In this context, a further step is to identify QTLs of resistance to this parasite. Therefore, a genetic map is first now being built. The establishment of a genetic linkage map will represent the basement for the mapping of QTLs, with the ultimate objective to implement marker-assisted selection in O....
Tipo: Text
Palavras-chave: Genetic map; Ostrea edulis; Genetic; European flat oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3472.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas - Contrat Région Poitou-Charentes 2000-2006. Convention 2004
Provedor de dados: 5
Autores: Bouilly, Karine; Bonnard, Marc; Lapegue, Sylvie.
Des anomalies cytogénétiques, telles que l'aneuploïdie, sont connues pour être communes chez les bivalves (Ahmed et Sparks, 1970; Dixon, 1982; Thiriot- Quiévreux, 1986; Martínez-Expósito et al., 1992; Li et Havenhand, 1997). Ce phénomène, qui a pour origine principalement une non-disjonction des chromosomes pendant la mitose ou la méiose (Bond et Chandley, 1983; Martin et Rademaker, 1990), est souvent létal chez les animaux supérieurs tels que les mammifères ou bien associé à un retard de croissance (Vig et Sandberg, 1987). Chez Crassostrea gigas, l’aneuploïdie est l’altération du nombre diploïde normal de 20 chromosomes (Ahmed et Sparks, 1967; Thiriot-Quiévreux, 1984) en cellules hypodiploïdes avec 19, 18 ou 17 chromosomes (Thiriot-Quiévreux et al.,...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00044/15477/12856.pdf

Surmortalités de la moule bleue Mytilus edulis dans les Pertuis Charentais (mars 2014)
Provedor de dados: 5
Autores: Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre.
Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf

Les ressources exploitées par la pêche et la conchyliculture
Provedor de dados: 5
Autores: Caill-milly, Nathalie; Morandeau, Gilles; Castege, Iker; Sanchez, Florence; Lissardy, Muriel; Chust, Guillem; Borja, Angel; Auby, Isabelle; Lapegue, Sylvie; Prou, Jean; Oger-jeanneret, Helene; Lassalle, Géraldine; Prevost, Etienne; Buoro, Mathieu; Lobry, Jérémy; De Pontual, Helene; De Casamajor, Marie-noelle; Biais, Gerard; D'Elbee, Jean.
L'exploitation des ressources par la pêche professionnelle et par la conchyliculture repose sur des ressources vivantes sauvages ou domestiquées qui sont présentes dans différents milieux aquatiques depuis les rivières jusqu'au large en mer. L'état de ces populations dépend non seulement des choix d'exploitation au regard des capacités de renouvellement des stocks mais aussi des caractéristiques environnementales qui impactent les différentes phases des cycles biologiques des espèces et jouent donc sur leur bon déroulement. Ces conditions du milieu sont tributaires pour partie des activités anthropiques (qualité et quantité d'eau disponible, obstacles au franchissement, autres perturbations) et peuvent être soumises aux effets du changement climatique. Ce...
Tipo: Text
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00444/55568/57180.pdf

Population genetics of the European flat oyster (Ostrea edulis) : from larvae to populations
Provedor de dados: 5
Autores: Boudry, Pierre; Launey, Sophie; Diaz-almela, E.; Naciri-graven, Yamama; Ledu, Christophe; Mira, Sara; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
Nowadays, PCR-based DNA markers allow the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European flat oyster (Ostrea edulis) populations has aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species.  An Atlantic/Mediterranean differentiation pattern was observed. Results were congruent between allozymes, microsatellites and mitochondrial DNA and  support an isolation by distance model. High variability was observed in the breadth of diversity between populations using a mitochondrial marker, this highlights the small effective population sizes in some locations. A 10-fold quantitative difference was observed in Fst...
Tipo: Text
Palavras-chave: Larvae; Reproduction; Genetic; Ostrea edulis; European flat oyster; Population genetics.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/acte-3493.pdf

Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Gagnaire, Beatrice; Bonnard, Marc; Thomas-guyon, Helene; Renault, Tristan; Miramand, Pierre; Lapegue, Sylvie.
Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cadmium on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in C. gigas at different stages of life. Adults and juveniles were exposed to two different concentrations of cadmium. The first concentration applied was equivalent to a peak value found in Marennes-Oleron bay (Charente-Maritime, France; 50 ng L-1) and the second was 10 times higher (500 ng L-1). Exposure to 50 ng L-1 cadmium caused a significant...
Tipo: Text
Palavras-chave: Phenoloxidase; Flow cytometry; Hemocytes; Aneuploidy; Crassostrea gigas; Cadmium.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1687.pdf

Impact of pollutants on aneuploidy in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Miramand, Pierre; Lapegue, Sylvie.
Aneuploidy is the alteration of the normal diploid chromosome number. In the Pacifie oyster, Crassastrea gigas, hypodiploid cells have regularly been reported as have a negative correlation between this phenomenon and growth and evidence for a genetic basis. We previously demonstrated a positive relationship between a pollutant, atrazine, and aneuploidy in Crassastrea gigas adults and juveniles. To evaluate the persistence of this impact, the present study focused on a sample of the same juveniles previously exposed to different atrazine treatments (0.0 1 mg/l which represents a peak value found in a polluted environment and 0.1 mg/l) for three and a half months. Then, we evaluated them for aneuploidy after another two and a half months in non polluted...
Tipo: Text
Palavras-chave: Cadmium; Atrazine; Pollutant; Pacific oyster; Aneuploidy.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2852.pdf

Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf

Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L.
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boudry, Pierre; Mira, Sara; Taris, Nicolas; Bonhomme, Francois.
In order to understand better some fundamental aspects of the bivalve biology, one has to pay attention to the variance of their breeding success, the time structure of the populations and their effective size. An abundant literature shows the existence of a heterozygote deficit as well as positive relationships between heterozygoty and fitness components. This last point is the subject of explanatory hypotheses which have never been clearly decided between until now. One of these hypotheses is the possible existence of a certain degree of inbreeding among those populations. This is only possible in the case of separated colonies coming from a small amount of spawners, thus creating blood relations between individuals recruited from the same area, and...
Tipo: Text
Palavras-chave: Reproducteur; Variabilité génétique; Génétique population; Diversité génétique; Ostrea edulis; Huître plate.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/acte-3494.pdf

Additive transcriptomic variation associated with reproductive traits suggest local adaptation in a recently settled population of the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Sussarellu, Rossana; Huvet, Arnaud; Lapegue, Sylvie; Quillen, Virgile; Lelong, Christophe; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre.
Background Originating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe.  Methods To explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60).  Results A female-biased sex-ratio and a higher condition index were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Adaptation; Invasiveness; Fertility; Sex-ratio; Transcriptome; Microarray.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00287/39829/38343.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas
Provedor de dados: 5
Autores: Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu'il existe, chez l'huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Anomalies; Aneuploïdie; Atrazine; Huîtres creuses; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00199/31013/29417.pdf

A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments
Provedor de dados: 5
Autores: Mccombie, Helen; Ledu, Christophe; Phelipot, Pascal; Lapegue, Sylvie; Boudry, Pierre; Gerard, Andre.
We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However,...
Tipo: Text
Palavras-chave: Oyster breeding; Gynogenesis; Polar body; Cytochalasin B; Polyploidy; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-632.pdf

Evidence for natural hybrtidisation between the two oyster sub-species Crassostrea gigas and Crassostrea angulata in Southern Europe
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie.
Crassostrea angulata and Crassostrea gigas, two cupped oyster taxa of Asian origin (O'Foighil et al, 1998), were successively introduced into Europe  during the XVIth  and the XXth century respectively (Héral and Grizel, 1991). To date, populations presumed to be C. angulata remain only in the south of Spain and Portugal, while the geographic range of C. gigas is expending, notably due to aquaculture. The two taxa can be distinguished by RFLP analysis of the mitochondrial Cytochrome Oxydase I locus (COI).  This marker revealed strong genetic differentiation between Asian populations of the two taxa (Boudry et al., 1998). In contrast, nuclear markers showed a much lower genetic differentiation (Huvet et al, in press). The absence of any reproductive barrier...
Tipo: Text
Palavras-chave: Microsatellite; Genetic; Crassostrea angulata; Crassostrea gigas; Oysters.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3453.pdf

Genetic Characterization of Cupped Oyster Resources in Europe Using Informative Single Nucleotide Polymorphism (SNP) Panels
Provedor de dados: 5
Autores: Lapegue, Sylvie; Heurtebise, Serge; Cornette, Florence; Guichoux, Erwan; Gagnaire, Pierre-alexandre.
The Pacific oyster, Crassostrea gigas, was voluntarily introduced from Japan and British Columbia into Europe in the early 1970s, mainly to replace the Portuguese oyster, Crassostrea angulata, in the French shellfish industry, following a severe disease outbreak. Since then, the two species have been in contact in southern Europe and, therefore, have the potential to exchange genes. Recent evolutionary genomic works have provided empirical evidence that C. gigas and C. angulata exhibit partial reproductive isolation. Although hybridization occurs in nature, the rate of interspecific gene flow varies across the genome, resulting in highly heterogeneous genome divergence. Taking this biological property into account is important to characterize genetic...
Tipo: Text
Palavras-chave: Cupped oysters; Population genetics; Introgression; Conservation; Shellfisheries.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00623/73544/72938.pdf

Impact of the reproduction dynamics on genetic variation in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to pursue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period; (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females; (3) the variance of individual...
Tipo: Text
Palavras-chave: Ostrea edulis; Oyster; Sexual reproduction; Population dynamics; Oyster culture; Inbreeding; Heterosis; Genetic isolation; Genetic diversity; Breeding success; Biological fertilization.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3436.pdf

Bonamia-ostreae induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas. Contrat Région Poitou-Charentes 2000-2006. Convention 99
Provedor de dados: 5
Autores: Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00043/15473/12852.pdf

Characterization of ten microsatellite loci in the blue mussel Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy R.
Mussels of the genus Mytilus are ecologically and commercially important worldwide, and they form hybrid complexes where their distributions overlap. Allozyme and nuclear markers have bee It Used to investigate their genetics over many years, but successful development of reliable highly valuable microsatellite markers has lagged be hind other shellfish species. We have developed and characterized tell novel microsatellite loci that amplify reliably for the blue mussel Mytilus edulis. The number of alleles among 30 individuals from a wild population (Menai Strait, North Wales UK) ranged between 9 and 29 and the observed heterozygosity between 0.300 and 0.954. Significant heterozygote deficiencies against the Hardy-Weinberg model were observed at 6 out of...
Tipo: Text
Palavras-chave: Mytilus edulis; Microsatellites; Universal tailed-primer labeling; Null alleles; Mussels.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00002/11275/7841.pdf

Recherche d'effets biologiques de l'atrazine sur hémocytes d'huître creuse, Crassostrea gigas, in vivo et in vitro
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Thomas-guyon, Hélène; Lapegue, Sylvie; Bouilly, Karine; Renault, Tristan.
In the last decades, shellfish farming and in particular ostreiculture have developed in a significant way in France. Marennes-Oleron (Charente-Maritime) basin is the first French producer of oysters. But this area is also subjected to many recurring pollution, brought mainly by the Charente river.  The recrudescence using of herbicides in agriculture including atrazine implies the transfer towards the aquatic environment of new pollutants in estuarine areas. Moreover industrial rejections bring many heavy metals in these same estuarine areas.  It is appropriate of wondering on harmful effects of all these pollutants on the animals living in these areas, particularly oysters.  Bivalve molluscs have been postulated as ideal indicator organisms because of...
Tipo: Text
Palavras-chave: Atrazine; Flow cytometry; Haemocytes; Crassostrea gigas; Atrazine; Cytométrie de flux; Hémocytes; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-834.pdf

Phylogeographic study of the dwarf oyster, Ostreola stentina, from Morocco, Portugal and Tunisia: evidence of a geographic disjunction with the closely related taxa, Ostrea aupouria and Ostreola equestris
Provedor de dados: 5
Autores: Lapegue, Sylvie; Ben Salah, Ines; Batista, Frederico; Heurtebise, Serge; Neifar, L; Boudry, Pierre.
Despite the economic importance of oysters due to the high aquaculture production of several species, the current knowledge of oyster phylogeny and systematics is still fragmentary. In Europe, Ostrea edulis, the European flat oyster, and Ostreola stentina, the Provence oyster or dwarf oyster, are both present along the European and African, Atlantic and Mediterranean, coasts. In order to document the relationship not only between O. stentina and O. edulis, but also with the other Ostrea and Ostreola species, we performed a sequence analysis of the 16S mitochondrial fragment (16S rDNA: the large subunit rRNA-coding gene) and the COI fragment (COI: cytochrome oxidase subunit I). Oysters were sampled from populations in Portugal (two populations), Tunisia...
Tipo: Text
Palavras-chave: Tunisia; Portugal; Marocco; Ostreola stentina; Ostreola equestris; Ostrea aupouria; Phylogeographic study.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1912.pdf

A first generation genetic linkage map of the European flat oyster Ostrea edulis (L.) based on AFLP and microsatellite markers
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),...
Tipo: Text
Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf

Point sur le programme huître plate - Complément au compte-rendu de réunion REGEMO sur l'Amélioration Génétique de l'huître plate du 11 mars 1998
Provedor de dados: 5
Autores: Goyard, Emmanuel; Lapegue, Sylvie; Gerard, Andre; Boudry, Pierre.
Ce document vient compéter le rapport REGEMO sur l'huître plate. Une première version visait essentiellement à faciliter le transfert de responsabilité du programme en cours. Celle-ci, épurée des aspects pratiques purement internes, se propose de faire le point sur les familles produites en 1998, et la continuité du programme d'amélioration.
Tipo: Text
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00049/15980/13422.pdf

Génomique de l'adaptation de l'huître creuse, Crassostrea gigas, dans le contexte de sa récente expansion géographique en Europe
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Originaire du nord de l’Asie, l'huître creuse, Crassostrea gigas, a été introduite et transférée, principalement pour l’aquaculture, dans plusieurs pays européens depuis le début des années 1970. Le caractère invasif de cette espèce a été observé au cours de la dernière décennie dans plusieurs pays et elle a même été considérée comme une espèce nuisible dans certaines régions, telles que la mer de Wadden. Dans le cadre du projet HiFlo, soutenu par l’ANR, nous avons pour objectif d'identifier les caractéristiques d'une telle espèce florissante: son succès peut-il être expliqué par le hasard des opportunités (expansion de leur niche écologique) ou est-il lié à des processus évolutifs, ce qui impliquerait une adaptation génétique de l'espèce à de nouvelles...
Tipo: Text
Palavras-chave: Huîtres; Crassostrea gigas; Génétique; Génétique des populations.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12552/9418.pdf

Development of four EST-SSR multiplex PCRs in the Pacific oyster (Crassostrea gigas) and their validation in parentage assignment
Provedor de dados: 5
Autores: Li, Ronghua; Li, Qi; Cornette, Florence; Degremont, Lionel; Lapegue, Sylvie.
We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously...
Tipo: Text
Palavras-chave: Pacific oyster; Crassostrea gigas; Microsatellite; EST; Multiplex PCR; Parentage assignment.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12419/9999.pdf

Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text
Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00341/45195/44592.pdf

AFLP-based genetic linkage maps of the blue mussel (Mytilus edulis)
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Hecquet, Celine; Boudry, Pierre; Beaumont, A.
We report the construction of the first genetic linkage map in the blue mussel, Mytilus edulis. AFLP markers were used in 86 full-sib progeny from a controlled pair mating, applying a double pseudo-test cross strategy. Thirty-six primer pairs generated 2354 peaks, of which 791 (33.6%) were polymorphic in the mapping family. Among those, 341 segregated through the female parent, 296 through the male parent (type 1:1) and 154 through both parents (type 3:1). Chi-square goodness-of-fit tests revealed that 71% and 73% of type 1:1 and 3:1 markers respectively segregated according to Mendelian inheritance. Sex-specific linkage maps were built with mapmaker 3.0 software. The female framework map consisted of 121 markers ordered into 14 linkage groups, spanning...
Tipo: Text
Palavras-chave: Mytilus edulis; Genetic linkage map; Blue mussel; AFLP.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2830.pdf

Oyster population genetics : understanding natural populations and tracing introductions
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boudry, Pierre.
Oysters are among the most familiar of all marine invertebrate taxa. However our knowledge of oyster phylogeny and systematics is fragmentary. This is principally due to the plastic growth patterns of these animals, which result in a wide range of overlapping, ecophenotypic variants that greatly reduce the value of analysis based on shell morphology. Besides that, many intentional or accidental anthropogenic transfers have emphasised this situation. In several cases of misclassification or misidentification of oysters, DNA molecular data, have provided valuable new insights on the tracing of introductions or more generally on the phylogeographic relationships between oyster species. We will illustrate this issue with recent case studies from our lab on...
Tipo: Text
Palavras-chave: Genetic; Ostreola stentina; Crassostrea honkongensis; Crassostrea gigas; Crassostrea angulata; Oysters; Population genetics.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3475.pdf

Chromosome loss in bi-parental progenies of tetraploid Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre.
Alterations of chromosome number have been observed in the somatic tissue of Crassostrea gigas diploids and artificial polyploids. Tetraploid oysters with abnormal chromosome numbers in some or all of their tissue are considered undesirable as parents either for triploids (produced via a cross with diploids and of aquacultural interest) or for tetraploid breeding. Aneuploid tetraploid oysters may confer the tendency to lose chromosomes and to revert to lower ploidy levels to their offspring. More directly, their offspring could have a lower ploidy because of potential links between somatic and gametic chromosome loss. The present study evaluated the phenomenon in six bi-parental tetraploid families bred from parents of differing somatic ploidy quality. The...
Tipo: Text
Palavras-chave: Crassostrea gigas; Mitosis; Chromosome; Cytogenetics; Aneuploid; Polyptoid; Tetraploid; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-633.pdf

Etude des anomalies chromosomiques chez Crassostrea gigas - Contrat Région Poitou-Charentes 2000-2006. Convention 2002
Provedor de dados: 5
Autores: Bouilly, Karine; Mccombie, Helen; Leitao, Alexandra; Lapegue, Sylvie.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Cette corrélation négative entre l’aneuploïdie somatique et le taux de croissance a été décrite à de nombreuses reprises (Thiriot-Quiévreux et al., 1988, 1992 ; Leitão et al., 2001a ; Zouros et al., 1996). Bien que les causes...
Tipo: Text
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00044/15475/12854.pdf

Development of SNP genotyping arrays in two shellfish species
Provedor de dados: 5
Autores: Lapegue, Sylvie; Harrang, Estelle; Heurtebise, Serge; Flahauw, Emilie; Donnadieu, C.; Gayral, P.; Ballenghien, M.; Genestout, L.; Barbotte, L.; Mahla, R.; Haffray, P.; Klopp, C.
Use of SNPs has been favored due to their abundance in plant and animal genomes, accompanied by the falling cost and rising throughput capacity for detection and genotyping. Here, we present in vitro (obtained from targeted sequencing) and in silico discovery of SNPs, and the design of medium-throughput genotyping arrays for two oyster species, the Pacific oyster, Crassostrea gigas, and European flat oyster, Ostrea edulis. Two sets of 384 SNP markers were designed for two Illumina GoldenGate arrays and genotyped on more than 1000 samples for each species. In each case, oyster samples were obtained from wild and selected populations and from three-generation families segregating for traits of interest in aquaculture. The rate of successfully genotyped...
Tipo: Text
Palavras-chave: Crassostrea gigas; GoldenGate technology; Ostrea edulis; Oysters; SNP genotyping.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00172/28369/26677.pdf

Taxonomie de l'huître naine Ostreola stentina
Provedor de dados: 5
Autores: Lapegue, Sylvie; Ben Salah, Inès; Batista, Frederico; Heurtebise, Serge; Neifar, Lassad; Boudry, Pierre.
Despite the economic importance of numerous oyster species throughout the world, our knowledge of their systematics and phylogeography remains fragmentary. In Europe, the indigenous species, Ostrea edulis, suffered overexploitation as well as two successive parasitosis over the last century. However, this species is still cultivated in Europe, and especially in Spain and France. Its distribution area stretches from the Atlantic coasts of Europe to the north of Africa as well as around the Mediterranean and Black Sea. Its Mediterranean and South Atlantic distribution area is shared with another indigenous species, the dwarf oyster, Ostreola stentina. Its very small adult size (a few centimetres) makes its culture pointless from a commercial point of view....
Tipo: Text
Palavras-chave: Ostreola stentina; Oyster; Taxonomy; Ostreola stentina; Huître; Taxonomie.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3364.pdf

Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie.
We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage...
Tipo: Text
Palavras-chave: Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6682.pdf

Recent advances in EST sequencing in Crassostrea gigas: towards the sequencing of the Pacific oyster genome
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud; Lapegue, Sylvie; Sauvage, Christopher; Tanguy, A.; Moraga, D.; Goostrey, A.; Prunet, P.; Boulo, Viviane; Gueguen, Y.; Moal, Jeanne; Fleury, Elodie; Samain, Jean-francois; Mathieu, M.; Favrel, P.
Rationale for EST and genome sequencing of the Pacific oyster: • its membership of the Lophotrochozoa, an little studied branch of the Eukaryotes, • its worldwide aquacultural importance (4.2 million metric tons, worth 3.5 billion US dollars), • its high fecundity, with concomitantly high DNA polymorphism, • its use as a biosensor of coastal pollution. In 2004, an international community of biologists, the Oyster Genome Consortium (OGC) led by D. Hedgecock (University of Southern California) and uniting 70 participants from 10 countries, first presented the Pacific oyster Crassostrea gigas as a genome-sequencing candidate to Joint Genome Institute (JGI, USA) (Hedgecock et al., 2005). Besides this project (that remains to be accepted), several EST...
Tipo: Text
Palavras-chave: Characteristics; Genome size; Genome; Crassostrea gigas; Oyster; Genetic.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3414.pdf

The taxonomic status and origin of the Portuguese oyster Crassostea angulata (Lamark, 1819)
Provedor de dados: 5
Autores: Batista, Frederico; Leitao, Alexandra; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre.
The taxonomic status of the Portuguese oyster Crassostrea angulata and the Pacific oyster Crassostrea gigas has often been a matter of controversy. Based on larval shell morphology, experimental hybridisation and electrophoretic studies of enzyme polymorphism several authors have considered these two species as being synonymous. During the recent years, several genetic studies based on mitochondrial DNA and microsatellites data have provided evidences that the two taxa are genetically distinct although closely related. Furthermore, karyotype analyses have also highlighted the close genetic similarity of these taxa in comparison with other cupped oyster species. However, a comparative analysis of restriction enzymes ideograms revealed differences between...
Tipo: Text
Palavras-chave: Geographical origin; Phylogenetic; Genetic; Taxonomic status; Crassostrea angulata; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3433.pdf

Apport d'un programme de génétique à une filière de production aquacole : l'exemple de l'ostréiculture
Provedor de dados: 5
Autores: Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre.
Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between...
Tipo: Text
Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf

Etude des ressources génétiques chez les huîtres creuses
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Lam, Katherine; Mccombie, Helen; Leitao, Alexandra; Gerard, Andre.
L'étude de la variabilité génétique de l'huître creuse Crassostrea gigas est un point essentiel dans la mise en place de programmes de gestion des ressources génétiques et de sélection chez cette espèce. C. gigas, originaire du Japon, a été introduite dans de nombreuses régions du monde et représente aujourd'hui plus de 95 'ro de la production mondiale d'huîtres. Introduite en France au début des années 1970 pour remplacer l'huître portugaise Crassostrea angulata, c'est aujourd'hui une ressource de première importance pour notre région. Malgré la maîtrise du cycle de reproduction de l'espèce et son importance commerciale, elle n'a pas été génétiquement améliorée et on peut donc considérer les stocks comme « sauvages» ou « non domestiqués ». De plus, la...
Tipo: Text
Palavras-chave: Génétique; Huîtres creuses; Crassostrea gigas; Crassostrea gasar; Variabilité génétique.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00199/31008/29411.pdf

Observatoire national du cycle de vie de l’huître creuse en France. Rapport annuel ECOSCOPA 2017
Provedor de dados: 5
Autores: Pouvreau, Stephane; Fleury, Elodie; Petton, Sebastien; Corporeau, Charlotte; Lapegue, Sylvie.
Occupant une place de premier ordre dans les paysages côtiers français, l’ostréiculture est le premier contributeur de la filière aquacole française avec une production estimée à 85 000 tonnes ces dernières années. Véritable espèce ingénieur, l’huître creuse, installée en France depuis plus de 50 ans, joue aussi un rôle essentiel sur le plan écologique dans les écosystèmes qui l’abritent. Pourtant, depuis plusieurs années, cette filière subie une baisse de production et les bancs naturels, garants de la diversité génétique, sont mis en danger. Ces phénomènes sont la conséquence directe de l’épizootie causée par OsHV-1 (sous sa forme µVar) affectant les naissains et des mortalités d’adultes associées à la bactérie Vibrio aestuarianus. Ces épisodes de...
Tipo: Text
Palavras-chave: Huître creuse; Physiologie; Génétique; Ecologie; Réseau National.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00449/56030/57556.pdf

The Proposed Dropping of the Genus Crassostrea for All Pacific Cupped Oysters and Its Replacement by a New Genus Magallana: A Dissenting View
Provedor de dados: 5
Autores: Bayne, B. L.; Ahrens, M.; Allen, S. K.; D'Auriac, M. Angles; Backeljau, T.; Beninger, P.; Bohn, R.; Boudry, Pierre; Davis, J.; Green, T.; Guo, X.; Hedgecock, D.; Ibarra, A.; Kingsley-smit, P.; Krause, M.; Langdon, C.; Lapegue, Sylvie; Li, C.; Manahan, D.; Mann, R.; Perez-paralle, L.; Powell, E. N.; Rawson, P. D.; Speiser, D.; Sanchez, J. -l.; Shumway, S.; Wang, H.
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00418/52944/53886.pdf

Le phénomène de l'anauploïdie et sa relation avec la croissance chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Leitao, Alexandra; Boudry, Pierre; Mccombie, Helen; Lapegue, Sylvie; Gerard, Andre; Thiriot-quievreux, Catherine.
Cytogenetic abnormalities have often been observed on bivalves on meiosis or mitosis. This is a review of various observations of somatic aneuploidy conducted on different populations of Crassastrea gigas.
Tipo: Text
Palavras-chave: Croissance; Aneuploïdie; Huîtres; Génétique; Crassostrea gigas; Cytogénétique.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3280.pdf

QTL mapping for resistance to bonamiosis in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
A breeding programme has been undertaken by Ifremer to produce selected Ostrea edulis families which are resistant to Bonamia ostreae, a parasite which is the most significant constraint on aquaculture of this oyster. Using such families, we aimed to identify Quantitative Trait Loci (QTLs) of resistance/susceptibility to bonamiosis. We used an F2 family derived from a bi-parental cross between a wild oyster and an individual from one of the selected oyster families. A total of 550 F2 individuals from the family cohabited with wild oysters that were injected with the parasite. Mortality was checked daily over six months and heart smears were used on moribund or dying oysters to detect the presence and level of infection of the parasite. Ninety two F2...
Tipo: Text
Palavras-chave: QTLs; Flat oyster; Ostrea edulis; Bonamia ostreae; Genetic; QTL.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3726.pdf

Persistence of atrazine impact on aneuploidy in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Mccombie, Helen; Leitao, Alexandra; Lapegue, Sylvie.
Aneuploidy is the alteration of the normal diploid chromosome number. In the Pacific oyster, Crassostrea gigas, hypodiploid aneuploid cells have regularly been reported as has a negative correlation between this phenomenon and growth and evidence for a genetic basis. We previously demonstrated a positive relationship between a pollutant, atrazine, and aneuploidy in Crassostrea gigas adults and juveniles. To evaluate the persistence of this impact, the present study focused on a sample of the same juveniles exposed to different atrazine treatments (0.01 mg/l which represents a peak value found in a polluted environment and 0.1 mg/l) for three and a half months and evaluated them for aneuploidy after another two and a half months in non polluted conditions....
Tipo: Text
Palavras-chave: Crassostrea gigas; Genetic; Pollutant persistence; Oyster culture; Marine molluscs; Herbicides; Cultured organisms; Chromosomes.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3344.pdf

Population genomics shed light on the demographic and adaptive histories of European invasion in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Crassostrea gigas originated from the Pacific coast of Asia, but was introduced into several European countries in the early 1970s. Natural populations have now spread across the length of the western seaboard of Europe. To elucidate the demographic and selective processes at play during this rapid expansion, genome-scan analysis was performed on different populations. High diversities and low differentiation were observed overall, but significant genetic differentiation was found among newly established populations and between the newly established northern group and a nearly panmictic group composed of southern European populations and a population from Japan. Loss of genetic diversity was also seen in the north, likely caused by founder events during...
Tipo: Text
Palavras-chave: AFLPs; Crassostrea gigas; Genome scan; Invasive species; Microsatellites; SNPs.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00149/25977/24070.pdf

Strategies for the retention of high genetic variability in European flat oyster (Ostrea edulis) restoration programmes
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Lapegue, Sylvie; King, Jon W.; Beaumont, Andy R.
The native European flat oyster Ostrea edulis is listed in the OSPAR Convention for the Protection of the Marine Environment of the North-East Atlantic (species and habitat protection) and in the UK Biodiversity Action Plan. Once extremely abundant in the 19th century, European stocks of O. edulis have declined during the 20th century to rare, small, localised populations due to overexploitation, habitat degradation and, most recently, the parasitic disease bonamiosis. Selective breeding programmes for resistance to bonamiosis have been initiated in France and Ireland. High genetic diversity and bonamiosis-resistance would be important features of any sustainable restoration programmes for O. edulis. Oysters were sampled across Europe from four hatchery...
Tipo: Text
Palavras-chave: Restoration programme; Ostrea edulis; Genetic variation; Pedigree reconstruction.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00001/11259/11375.pdf

Exploring local reproductive differentiation of Pacific oyster population recently settled in northern Europe by transcriptomic approach
Provedor de dados: 5
Autores: Sussarellu, Rossana; Huvet, Arnaud; Lelong, Christophe; Pernet, Fabrice; Quillen, Virgile; Lapegue, Sylvie; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre.
Originating from north-eastern Asia, the Pacific oyster Crassostrea gigas has been introduced, mainly for aquaculture purpose, into a large number of countries. Although highly variable, its invasive nature has been reported in an increasing number of coastal areas, notably. Northern Europe. We set up a common garden experiment based on the comparison of progenies of Pacific oysters sampled in France, where populations settled since the introduction of the species in the 70’s, and Denmark, where established populations were observed since the beginning of 90s. Pure and hybrid progenies, resulting from crosses within and between adults sampled in both populations, were studied for their sex-ratio, condition index, transcriptomics, and biochemical parameters
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00167/27815/26008.pdf

Application of high-throughput sequencing to population differentiation in the cupped oysters Crassostrea angulata/C. gigas
Provedor de dados: 5
Autores: Gagnaire, Pierre-alexandre; Cornette, Florence; Heurtebise, Serge; Auge, M. T.; Bierne, Nicolas; Lapegue, Sylvie.
The cupped oysters Crassostrea angulata and Crassostrea gigas have been sequentially introduced in Europe from their native Pacific area for aquaculture purposes. Ongoing viral epidemic disease is responsible for large losses in oyster aquaculture production throughout Europe. In parallel with current efforts in developing selection programs for disease resistance, it becomes critically important to characterize genetic diversity patterns in both native and introduced areas. With the advent of next generation sequencing techniques, the power to resolve the fine-scale genetic structure at a genome-wide scale opens to new perspectives towards genetic resource management. Here, we present a genome-wide screen of genetic variation that combines SNP genotyping...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00167/27816/26009.pdf

Analysis of genome-wide differentiation between native and introduced populations of the cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie.
The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end...
Tipo: Text
Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00455/56622/58337.pdf

Persistence of atrazine impact on aneuploidy in Pacific oysters, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Mccombie, Helen; Leitao, Alexandra; Lapegue, Sylvie.
Widespread use of the herbicide atrazine has incited much research on its toxicity in aquatic systems, where it is routinely detected due to runoff from cultivated fields. Moreover, the determination of the genotoxic effect of such pollutants in the marine environment has become a major requirement for ecosystem protection. In the Pacific oyster, Crassostrea gigas, hypodiploid aneuploid cells have regularly been reported. There is a negative correlation between this phenomenon and growth, as well as evidence for a genetic basis. A positive relationship between atrazine and aneuploidy has previously been demonstrated in C. gigas adults and juveniles. To evaluate the persistence of this impact, our study examined the offspring of the same adult population...
Tipo: Text
Palavras-chave: Aneuploidy; Crassostrea gigas; Atrazine; Toxicity; Pollutant persistence.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-580.pdf

Etude de faisabilité d’opérations de ‘repeuplement orienté’ dans deux sites français de captage de l’huître creuse (Crassostrea gigas)
Provedor de dados: 5
Autores: Bernard, Ismael; Lapegue, Sylvie; Dumas, Franck; Talarmain, Eric; Cornette, Florence; Degremont, Lionel; Chavanne, Herve; Pouvreau, Stephane; Auby, Isabelle; Maurer, Daniele; Plus, Martin; Le Moine, Olivier; Boudry, Pierre.
La forte augmentation des mortalités de juvéniles d’huître creuse depuis 2008 a relancé la problématique de l’approvisionnement en naissains de l’ostréiculture française. Dans ce contexte, cette étude a pour objectif d’étudier la faisabilité d’opérations de repeuplement en huîtres génétiquement plus résistantes, afin d’améliorer les performances de survie du naissain capté dans le milieu naturel. Cette étude s’est ainsi focalisée sur les deux bassins naisseurs historiques, Marennes-Oléron et Arcachon. Nous avons donc cherché à établir (1) une hiérarchie des bancs en fonction de l’expulsion de larves d’huître dans le bassin d’Arcachon, (2) une cartographie des liens en termes de connectivité entre les bancs des pertuis charentais et (3) la faisabilité du...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00083/19410/17013.pdf

Mitochondrial and Nuclear DNA Analysis of Genetic Heterogeneity Among Recruitment Cohorts of the European Flat Oyster Ostrea edulis
Provedor de dados: 5
Autores: Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Camara, Mark D.; Lapegue, Sylvie.
Marine species with high fecundity and high early mortality may also have high variance in reproductive success among individuals due to stochastic factors, making successful reproduction a "sweepstakes." In some cases, the impact is sufficient to reduce the effective number of breeders in wild populations. We tested two predictions of the sweepstakes reproductive success hypothesis in a French Atlantic population of the European flat oyster, Ostrea edulis, by evaluating (1) whether individuals belonging to temporally discrete recruitment cohorts within a single reproductive season displayed reduced genetic variation relative to the entire adult population, and (2) whether these temporal cohorts of recruits were genetically differentiated from each other....
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-7399.pdf

Evaluation of the gonad volume throughout magnetic resonance imaging in Crassostrea gigas and comparison with the histological method
Provedor de dados: 5
Autores: Flahauw, Emilie; Davenel, Armel; Quellec, Stéphane; Quillien, Virgile; Degremont, Lionel; Lapegue, Sylvie; Hatt, Philippe-jacques.
Biological parameters of the cupped oyster Crassostrea gigas such as growth, reproductive effort (duration of the gametogenesis, date of spawning, and fecundity) differ widely among oysters in the same location. Furthermore, most of the traditional methods, like histological observations, could lead to large variability on the observations and the measurements ofthese traits, and large uncertainties on their changes over time. These can be drastically reduced by using Magnetic Resonance Imaging (MRI) which is a non invasive method allowing to measure the same individual during its development. Forty oysters were assessed using MRI for the volume of whole soft body, the intra valves space, the adductor muscle, and the gonad, which also include the digestive...
Tipo: Text
Palavras-chave: Molluscs; Crassostrea gigas; Gonad; Histology; MRI.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00107/21784/19370.pdf

Genetic characterisation of oyster populations along the north-eastern coast of Tunisia
Provedor de dados: 5
Autores: Dridi, S.; Romdhane, M. S.; Heurtebise, Serge; El Cafsi, M.; Boudry, Pierre; Lapegue, Sylvie.
The taxonomy of oysters has been traditionally based on characteristics of the shell. More recently, the analysis of protein and DNA polymorphism has provided a means to overcome difficulties in distinguishing the different species of oysters based solely on shell morphology. In order to identify oysters of the Tunisian north-east coast, we sequenced a 16S rRNA mitochondrial fragment from 68 oysters sampled from the Bizert Lagoon and the Gulf of Hammamet in northern Tunisia. Comparison of oyster 16S rRNA sequences available in GenBank showed the presence of both Ostreola stentina and Crassostrea gigas in our samples, which could not be detected on the basis of shell morphology only. These data confirmed that C. gigas, a non-native species, is now...
Tipo: Text
Palavras-chave: 16S rRNA; Crassostrea gigas; Genetics; Mediterranean Sea; Ostreola stentina; Polymorphism.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00000/11063/7820.pdf

Genetic impact of the reproduction dynamics in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual...
Tipo: Text
Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huîtres creuses Crassostrea gigas par sélection de souches performantes - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 97 RPC-R-54 "Génétique" - Rapport année 1997
Provedor de dados: 5
Autores: Boudry, Pierre; Lapegue, Sylvie; Goyard, Emmanuel; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1997 étaient les suivants : • Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. • Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant limitée).
Tipo: Text
Palavras-chave: Génétique; Génétique des Populations; Acclimatation; Hybridation; Marqueurs génétiques; Sélection génétique; Crassostrea gigas.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00044/15519/12906.pdf

A first genetic linkage map of the blue mussel Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Hecquet, Céline; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy.
The blue mussel, Mytilus edulis, is widely distributed in the northern hemisphere, from the White Sea to the Atlantic coast of southern France, and is an economically important species (1 million tons per year, FAO, 2002). At present however, little domestication has been achieved with bivalves, in contrast to agricultural crops, livestock and horticultural species like tomatoes, where selection and production is entirely controlled by man. For bivalves, despite their economic importance, no lines with selected characteristics have been taken to the production scale. There is therefore a need to make up for lost time. Our interest in genetic mapping sprang from some results showing a genetic component to some economically important traits like growth and...
Tipo: Text
Palavras-chave: QTLs; AFLP; Genetic; Mussel; Mytilus edulis; Genetic linkage map.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3465.pdf

Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification
Provedor de dados: 5
Autores: Danic-tchaleu, Gwenaelle; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie.
Background  Because of its typical architecture, inheritance and small size, mitochondrial (mt) DNA is widely used for phylogenetic studies. Gene order is generally conserved in most taxa although some groups show considerable variation. This is particularly true in the phylum Mollusca, especially in the Bivalvia. During the last few years, there have been significant increases in the number of complete mitochondrial sequences available. For bivalves, 35 complete mitochondrial genomes are now available in GenBank, a number that has more than doubled in the last three years, representing 6 families and 23 genera. In the current study, we determined the complete mtDNA sequence of Ostrea edulis, the European flat oyster. We present an analysis of features of...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00045/15628/13031.pdf

Phylogeography of mangrove oysters from the Southern Atlantic ocean : Crassostrea gasar and Crassostre rhizophorae
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Thiriot-quievreux, Catherine; Heurtebise, Serge; Garcia, P.; Boudry, Pierre.
Mangrove oysters encompass several species and their taxomony is unclear. Among then, Crassostrea rhizophorae has been described along the Central and South American Atlantic coasts and Crassostrea gasar along African Atlantic coasts. Most taxonomic studies have so far been based on morphological criteria which are particularly susceptible to plasticity and new insight can be provided by molecular markers. The present work aims to provide information on the phylogeography of these two taxa.
Tipo: Text
Palavras-chave: Genetic; Phylogeography; Crassostrea rhizophorae; Crassostrea gasar; Mangrove oysters.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3496.pdf

Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe
Provedor de dados: 5
Autores: Huvet, Arnaud; Lapegue, Sylvie; Magoulas, A; Boudry, Pierre.
The respective status of the Portuguese oyster, Crassostrea angulata, and the Pacific oyster, Crassostrea gigas, has long been a matter of controversy. Morphological and physiological similarities, homogeneity of allozyme allelic frequencies between populations of the two taxa and the demonstration of hybridization lead most authors to suggest that they should be regrouped within the same species. The risk of introgression and the present expansion of C. gigas aquaculture in Europe raises the question of the need for preservation of C. angulata in Europe, as only a few populations remain. We studied European and Asian populations of C. gigas and C. angulata using microsatellite and mitochondrial DNA markers to estimate their genetic diversity and...
Tipo: Text
Palavras-chave: Phylogeography; Population genetics; Microsatellites; Crassostrea gigas; Crassostrea angulata.
Ano: 2000
URL: https://archimer.ifremer.fr/doc/2000/publication-707.pdf

GenoPopTaille. Moving stock assessment into the future
Provedor de dados: 5
Autores: Marandel, Florianne; Biais, Gerard; Bidault, Adeline; Charrier, Grégory; Cornette, Florence; Lapegue, Sylvie; Laroche, Jean; Le Cam, Sabrina; Stephan, Eric; Trenkel, Verena; Lorance, Pascal.
At the ecosystem level, sustainable exploitation of fisheries resources depends not only on the status of target species but also on that of bycatch species, some of which are even more sensitive to exploitation. This is the case for a number of elasmobranchs (skates, rays and sharks) species whose abundance declined during the 20th century. Further, the biology of elamobranchs is still poorly known and traditional fisheries stock assessment methods using fisheries catches and scientific survey data for estimating abundance are expensive or even inapplicable due to the small numbers observed. The GenoPopTaille project attempts to apply to the case of the thornback ray (Raja clavata) recent genetic-based methods for absolute population abundance estimation...
Tipo: Text
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00343/45439/44960.pdf

Reduced female gene flow in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Diaz Almela, F; Boudry, Pierre; Launey, Sophie; Bonhomme, Francois; Lapegue, Sylvie.
The geographical structure of 15 natural populations of the flat oyster (Ostrea edulis L.) was assessed by single-strand conformation polymorphism (SSCP) of a 313-base-pair (bp) fragment of the mitochondrial 12S-rRNA gene. Fourteen haplotypes were observed, with one being dominant in the Mediterranean samples and another one in the Atlantic populations. The geographically extreme populations sampled in Norway and the Black Sea appeared differentiated by exhibiting the dominance of a third group of haplotypes. The results were compared to available microsatellite data at five loci. The Atlantic/Mediterranean differentiation pattern was qualitatively the same with both types of markers, confirming an isolation-by-distance pattern. The average mitochondrial...
Tipo: Text
Palavras-chave: Ostrea edulis; Polymorphism; Flat oyster; Population; Gene.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-680.pdf

VIVALDI, A H2020 European Project aiming at preventing and mitigating farmed bivalve diseases
Provedor de dados: 5
Autores: Arzul, Isabelle; Feist, Steve; Figueras, Antonio; Lapegue, Sylvie; Paillard, Christine; Furones, Dolors.
The European shellfish industry enjoys a privileged position on the global scene. Its social dimension is essential, as it employs a high number of people in more than 8000 companies, mostly micro-companies. Shellfish production in Europe is little diversified and mainly relies on the industrially produced mussels, oysters and clams. Over the recent years, this sector has grown more slowly than other fish farming sectors, notably because it depends a great deal on the environmental quality and the emergence of diseases. Mortality events, linked to pathogen organisms such as viruses, bacteria and parasites (protozoa), tend to weaken the production’s sustainability. In this context, the European project VIVALDI (PreVenting and mItigating farmed biVALve...
Tipo: Text
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00352/46307/45975.pdf

Past and present geographical distribution of populations of Portuguese (Crassostrea angulata) and Pacific (C. gigas) oysters along the European and north African Atlantic coats
Provedor de dados: 5
Autores: Fabioux, Caroline; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre.
Today, it is commonly accepted that C. angulata and C. gigas are a single species according to morphological, genetic and experimental hybridization data. Following the viral disease that affected C. angulata and the subsequent expansion of C. gigas in Europe, it was of interest to examine the geographical distribution of both taxa in Europe. We studied the genetic composition of seventeen populations of cupped oysters, sampled in France, Spain, Portugal, Morocco and Italy, using a diagnostic marker, the mitochondrial Cytochrome Oxydase I gene. Results showed two distinct geographical zones. All the populations located in the northern part of the European sampled zone were mainly composed of C. gigas haplotypes whereas populations from southern Europe and...
Tipo: Text
Palavras-chave: Geographical distribution; Crassostrea angulata; Crassostrea gigas; Distribution géographique; Crassostrea angulata; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2785.pdf

"Bivalvomix" : génomique évolutive des bivalves marins
Provedor de dados: 5
Autores: Bierne, Nicolas; Boudry, Pierre; Lapegue, Sylvie; Bonhomme, Francois; Faure, M; Sauvage, Christopher; Moraga, D; Boutet, Isabelle; David, Elise; Jollivet, Didier; Tanguy, Arnaud; Faure, Baptiste; David, Patrice.
For a long time, population geneticists have had to content themselves with analysing neutral markers to infer selection processes indirectly. The recent improvement of sequencing tools now enables them to analyse the variations of a large number of genes and therefore to spot the genes, or the amino acids, which are under the direct influence of the selection (Yang &amp; Bielawski, 2000). In order to analyse the selection processes acting on the mutations and discriminate the different factors acting on their evolution (genetic selection or drift), it is essential to compare polymorphism data between genomes of the same species/population and divergence data between species/populations (Kimura, 1983). Recently, polymorphism data has become available for a...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-6489.pdf

Différenciation génétique des populations d'huître plate européenne, Ostrea edulis, à l'aide de marqueurs des génomes mitochondrial et nucléaire
Provedor de dados: 5
Autores: Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois.
Two oyster species are cultivated on the French coasts: the Pacific cupped oyster (Crassostrea gigas), which has been introduced from Japan in the 1970's after the disappearance of the Portuguese oyster Crassostrea angulala, and the European flat oyster (Ostrea edulis). During the second half of the 1960's, the latter suffered successively from two diseases caused by protozoan parasites: marteiliosis (also called "maladie des Abers") and bonamiosis. In France, its production dropped from 20,000 tian in the 1960's to 2,000 today. The impact of these two pathogens as well as the exploitation of the remaining stocks show the importance of the conservation of this species, the only natural European species, whose population is more and more fragmented.
Tipo: Text
Palavras-chave: Marqueurs; Génétique population; Génétique; Ostrea edulis; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3273.pdf

Variance in the reproductive success of flat oyster Ostrea edulis L. assessed by parentage analyses in natural and experimental conditions
Provedor de dados: 5
Autores: Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie.
In order to document further the phenomena of variance in reproductive success in natural populations of the European flat oyster Ostrea Midis, two complementary studies based on natural and experimental populations were conducted. The first part of this work was focused on paternity analyses using a set of four microsatellite markers for larvae collected from 13 brooding females sampled in Quiberon Bay (Brittany, France). The number of individuals contributing as the male parent to each progeny assay was highly variable, ranging from 2 to more than 40. Moreover, paternal contributions showed a much skewed distribution, with some males contributing to 50-100% of the progeny assay. The second part of this work consisted of the analysis of six successive...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00012/12329/9222.pdf

The use of SNPs in characterizing oysters genomes and their resistance to pathogens
Provedor de dados: 5
Autores: Lapegue, Sylvie; Heurtebise, Serge; Harrang, Estelle; Morga, Benjamin; Flahauw, Emilie; Sauvage, Christopher; Boudry, Pierre.
During the last five years, a great effort has been made to develop genomic tools in oyster species all over the world. In Europe, the Pacific cupped oyster, Crassostrea gigas, and the European flat oyster, Ostrea edulis received a special attention as they are the key species for local aquaculture. In particular, the Pacific oyster is a marine bivalve of major economical and ecological importance worldwide. Better knowledge of its physiology and its genetics contributes to optimize its aquaculture production using selective breeding or to understand its invasive behavior in some coastal areas. Current international developments of genomic resources include EST databases, BAC libraries, genome mapping and sequencing. The Genoscope (Evry, France) and the...
Tipo: Text
Palavras-chave: Oysters; Crassostrea gigas; Ostrea edulis; Resistance pathogens; QTLs; Single Nucleotide Polymorphism.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12584/9454.pdf

Apport de la génétique pour une meilleure connaissance des populations naturelles et une amélioration des populations cultivées d'huîtres plates et creuses
Provedor de dados: 5
Autores: Lapegue, Sylvie.
In the framework of the French diploma "Habilitation à Diriger des Recherches", Sylvie Lapègue's synthesis of research includes ten years (1998-2007) of work in bivalve genetics, and mainly cupped and flat oysters. This research has been developed in (1) flat oyster population genetics at the European level and cupped oyster populations and species genetics at a global scale, (2) the characterization of a cytogenetic particularity observed in oysters that corresponds to a high rate of aneuploidy in somatic cells, and (3) more recently in the use of molecular markers for the construction or improvement of genetic maps, allowing (4) the identification of areas of the genome related to the resistance to summer mortality in the Pacific oyster and the...
Tipo: Text
Palavras-chave: Ostrea; Crassostrea; Oysters; QTLs; Genetic maps; Breeding; Aneuploidy; Population genetics; Ostrea; Crassostrea; Huître; QTLs; Cartes génétiques; Amélioration; Aneuploïdie; Génétique des populations.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/these-3478.pdf

Mitochondrial and microsatellite genetic differentiation of the flat oyster Ostrea edulis along the european coasts, and comparison with allozyme data
Provedor de dados: 5
Autores: Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois.
Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in  the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes.
Tipo: Text
Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf

Phylogéographie des huîtres de mangrove de l'Océan Atlantique Sud : C. gasar et C. rhisophorae
Provedor de dados: 5
Autores: Heurtebise, Serge; Boutet, Isabelle; Verden, B.; Lapegue, Sylvie; Leitao, Alexandra; Thiriot-quievreux, Catherine; Garica, P.; Boudry, Pierre.
Mangrove oysters include various species that are also present on different coasts. In the southern part of the Atlantic Ocean, Crassostrea rhizophorae has been observed along the southern coast of America and Crassostrea gasar along the western coast of Africa. Fifteen populations of these oysters were sampled from these two geographic areas. Their polymorphism was studied on the 16S mitochondrial fragment both by par sequencing and RFLP analysis. Some samples' caryotype were also analysed. Two haplotypes were identified: C. gasar was found in Africa, but also for the first time in South America, whereas C. rhizophorae was only found on the coasts of South America.
Tipo: Text
Palavras-chave: Crassostrea rhizophorae; Crassostrea gasar; Huîtres; Génétique; Phylogéographie.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3278.pdf

A preliminary genetic linkage map for the European flat oyster Ostrea eduli
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Halley, Chris; Boudry, Pierre; Beaumont, Andy.
The European flat oyster, Ostrea edulis, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and throughout the Mediterranean. The oyster has been a harvested species for at least 6000 years. However, it successively suffered from over harvesting and then from two protozoan diseases during the 1960s and its production has drastically decreased in Europe from that time. Following two generations of selection for tolerance to one of these diseases (bonamiosis), our aim is to optimize this selection with the assistance of molecular markers, that is to say to implement marker-assisted selection. This will enhance the efficiency of selection by reducing the generation time, as the selection on sites cannot be made before the oysters are...
Tipo: Text
Palavras-chave: QTLs; Ostrea edulis; Genetic linkage map; Genetic; European flat oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3473.pdf

Phylogeography of the green turtle, Chelonia mydas, in the Southwest Indian Ocean
Provedor de dados: 5
Autores: Bourjea, Jerome; Lapegue, Sylvie; Gagnevin, L; Broderick, D; Mortimer, J.a.; Ciccione, S; Roos, David; Taquet, Coralie; Grizel, Henri.
Patterns of mitochondrial DNA (mtDNA) variation were used to analyse the population genetic structure of southwestern Indian Ocean green turtle (Chelonia mydas) populations. Analysis of sequence variation over 396 bp of the mtDNA control region revealed seven haplotypes among 288 individuals from 10 nesting sites in the Southwest Indian Ocean. This is the first time that Atlantic Ocean haplotypes have been recorded among any Indo-Pacific nesting populations. Previous studies indicated that the Cape of Good Hope was a major biogeographical barrier between the Atlantic and Indian Oceans because evidence for gene flow in the last 1.5 million years has yet to emerge. This study, by sampling localities adjacent to this barrier, demonstrates that recent gene...
Tipo: Text
Palavras-chave: Phylogeography; Mozambique Channel; Mitochondrial DNA; Indian Ocean; Control region; Chelonia mydas.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2185.pdf

Evidence for the presence of the Portuguese oyster, Crassostrea angulata, in northern China
Provedor de dados: 5
Autores: Lapegue, Sylvie; Batista, Frederico; Heurtebise, Serge; Yu, Ziniu; Boudry, Pierre.
The Pacific oyster, Crassostrea gigas (Thunberg), and the Portuguese oyster, C. angulata (Lamarck), are two closely related taxa. Although these two taxa were both introduced from Asia into Europe. one (C. gigas) was voluntarily introduced in the early 1970s, whereas the other (C. angulata) was presumed to be present in Europe for at least four centuries, but nearly disappeared because of disease. Few C. angulata populations remained in southern Portugal, Spain and Morocco and their putative origin was traced in Taiwan. The present paper reports evidence for its presence in Northern China. We reanalyzed recently published mitochondrial cytochrome oxidase C subunit I (COI) sequence data from presumed Dalianwan oysters (C. talienwhanensis) and compared them...
Tipo: Text
Palavras-chave: Phylogeography; Cytochrome oxidase C subunit 1 gene; Crassostrea talienwhanensis; Gigas; Crosstrea; Crassostrea angulata; Cupped oysters.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-3173.pdf

Ressources génétiques chez l'huître creuse Crassostrea gigas : variabilité, différenciation et adaptation aux conditions de production en Charente Maritime
Provedor de dados: 5
Autores: Huvet, Arnaud; Boudry, Pierre; Baud, Jean-pierre; Nourry, Max; Phelipot, Pascal; Gerard, Andre; Ledu, Christophe; Heurtebise, Serge; Cornette, Florence; Blouin, Frederic; Bedier, Edouard; Lapegue, Sylvie.
es marqueurs génétiques, particulièrement bien adaptés à ces problématiques car hypervariables, ont été mis au point récemment dans notre laboratoire en collaboration avec le laboratoire Génome et Populations de Montpellier (CNRS). Ils permettent désormais l'analyse approfondie et la discrimination fine de populations d'huîtres creuses Crassostrea gigas et de rechercher si certaines d'entre-elles n'auraient pas des caractéristiques intéressantes pour l'ostréiculture locale (croissance, résistance estivale, affinage ... ). La diffusion de telles populations auprès de la profession serait plus rapide car il s'agit de la même espèce. Selon les recommandations du CIEM, il faut effectuer une mise en quarantaine des huîtres importées (après contrôles sanitaires)...
Tipo: Text
Palavras-chave: Génétique; Génétique des populations; Marqueurs génétiques; Microsatellite; Huître creuse; Crassostrea gigas.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00044/15563/12952.pdf

Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas)
Provedor de dados: 5
Autores: Sauvage, Christopher; Lapegue, Sylvie; Bierne, Nicolas; Boudry, Pierre.
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). The identification of markers associated to the resistance would allow to better understand this complex phenomenon and, eventually to establish markers assisted selection. Recently, two moderately dense genetic linkage maps have been established in the Pacific oyster based on microsatellites and AFLP markers (Hubert and Hedgecock, 2004; Li and Guo, 2004). In this context, the development and mapping of SNPs will increase the density of the linkage map and consequently the...
Tipo: Text
Palavras-chave: QTLs analyses; Summer mortaliy; Genetic; SNPs; Crassostrea gigas; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3351.pdf

Compte rendu de la réunion REGEMO sur l'amélioration génétique de l'huître plate du 11 mars 1998 à Nantes : Bilan actualisé du programme de sélection
Provedor de dados: 5
Autores: Goyard, Emmanuel; Lapegue, Sylvie; Cochennec, Nathalie; Baud, Jean-pierre; Langlade, Aime; Joly, Jean-pierre; Buestel, Dominique; Oheix, Jocelyne; Haure, Joel.
Le programme de sélection de l'huître plate a été initié en 1985 par des pathologistes de la Tremblade. La sélection ne portait que sur le seul critère de tolérance des huîtres plates au parasite Bonamia ostreae et correspondait plus à un outil pour étudier divers aspects de la parasitose qu'un un programme d'amélioration génétique visant la relance de la culture de l'huître plate. Dans le cadre du réseau REGEMO, ce programme a ensuite été repris par les généticiens de l'Ifremer : 1) rappel historique et définition d'un vocabulaire commun à l'ensemble des membres du réseau REGEMO. 2) Inventaire et bilan des opération menées ou en cours ( sur la dernière génération de sélection produite ne 1995, sur le matériel issu de cette génération et produit en 1997....
Tipo: Text
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00049/15977/13419.pdf

Pacific cupped oyster - Crassostrea gigas
Provedor de dados: 5
Autores: Lapegue, Sylvie; Boudry, Pierre; Goulletquer, Philippe.
Originating from the north eastern Asia, Crassostrea gigas is endemic to Japan, but has been introduced and translocated, mainly for aquaculture purpose, into several countries, almost worldwide (1). In North America, the species can be found from Southeast Alaska to Baja California, while in European waters the species is cultured from Norway to Portugal as well as in Mediterranean Sea (Fig.1) (2). Biological characteristics make it suitable for a wide range of environmental conditions, although it is usually found in coastal and estuarine areas within its natural range. Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (3).
Tipo: Text
Palavras-chave: Genetics; Ecology; Crassostrea gigas; Biology; Oysters.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3322.pdf

Observations histologiques et analyses en hybridation in situ de bénitiers Tridacna maxima
Provedor de dados: 5
Autores: Arzul, Isabelle; Lapegue, Sylvie; Renault, Tristan.
Tipo: Text
Ano: 2013
URL: https://archimer.ifremer.fr/doc/00156/26683/81049.pdf

Rapport d'activités 2015 de l'Unité SG2M : Santé, Génétique et Microbiologie des Mollusques
Provedor de dados: 5
Autores: Lapegue, Sylvie; Renault, Tristan; Bechemin, Christian; Le Guyader, Soizick; Stavrakakis, Christophe; Rivet, Florence.
L’unité SG2M est structurée pour aborder de manière intégrée les domaines de la santé animale et de la santé humaine liée à production, à la consommation de coquillages, et à la qualité microbiologique du littoral. Ainsi, dans le cadre d’un continuum recherche/surveillance/expertise, et d’une approche pluridisciplinaire, l’unité cherche à développer des connaissances concernant les interactions entre agents infectieux, hôtes et environnement chez les mollusques marins, et les cycles de contamination. Dans le domaine de la recherche, les activités de l’unité sont déclinées selon quatre axes structurants : (1) la microbiologie sanitaire et les voies de transfert des agents pathogènes humains dans l’environnement côtier, (2) l’étude des maladies affectant les...
Tipo: Text
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00319/43055/42562.pdf

European flat oyster - Ostrea edulis
Provedor de dados: 5
Autores: Lapegue, Sylvie; Beaumont, A.; Boudry, Pierre; Goulletquer, Philippe.
The European flat oyster, Ostrea edulis, a native of Europe, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and in the whole Mediterranean Basin; It has been a harvested species for at least 6000 years. Natural populations are also observed in eastern North America, from Maine to Rhode Island, following intentional introductions in the 1940s and 1950s. The species was also introduced in Canada for aquaculture purpose 30 years ago and some populations naturalised in Nova Scotia, New Brunswick and British Columbia. These stocks were imported from naturalised populations in Maine whose ancestors originated in the Netherlands.
Tipo: Text
Palavras-chave: Genetics; Ecology; Biology; Ostrea edulis; Oysters.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3321.pdf

Review and practical use of genome mapping in aquacultured organisms. Position paper by the Working Group on the Application of Genetics in Fisheries and Mariculture (WGAGFM)
Provedor de dados: 5
Autores: Boudry, Pierre; Lapegue, Sylvie; Guyomard, R.
The Working Group on the Application of Genetics in Fisheries and Mariculture (WGAGFM) met in La Rochelle, France from 10-12 March, 2003. Seven persons representing six countries were present. Three terms of reference (ToR) were addressed, while one was deferred until the appropriate information was available for review. WGAGFM first considered ToR a) Review and report on the practical use of genome mapping in aquacultured organisms. Genome maps are used extensively in the production of selected strains of agriculture plants and livestock. Studies on the genome of aquatic animais have been summarised in the WG report. Genome mapping projects in aquaculture exist for most major species and linkage maps are now becoming available. WGAGFM produced four...
Tipo: Text
Palavras-chave: Conservation of genetic diversity; Triploids; Recommandation; Aquaculture; Fish; Genome mapping; Genetic.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3301.pdf

Scanning the genome of the Pacific cupped oyster, Crassostrea gigas, for adaptative differentiation during geographic range expansion
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf

Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
Provedor de dados: 5
Autores: Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: Text
Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf

Diversité et différentiation génétique chez l'huître plate européenne : de la population à l'individu (Actes)
Provedor de dados: 5
Autores: Boudry, Pierre; Launey, Sophie; Diaz-almela, A.; Naciri-graven, Yamama; Ledu, Christophe; Mira, S.; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
PCR-based DNA markers enable the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European fiat oyster (Ostrea edulis) populations aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Similar patterns of variation were found using allozymes, microsatellites and mitochondrial DNA, aIl which support an isolation by distance model. High variability was observed in the range of diversity between populations using a mitochondrial marker, a reflection of small effective population sizes in sorne locations. A 10fold quantitative difference was observed in Fst,...
Tipo: Text
Palavras-chave: Recruitment; Diversity; Molecular markers; Population genetics; Ostrea edulis; Flat oyster; Dynamique de recrutement; Diversité génétique; Microsatellites; Marqueurs moléculaires; Génétique des populations; Ostrea edulis; Huître plate.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2851.pdf

Etude du niveau d'aneuploïdie dans les populations des zones de captage du bassin de Marennes-Oléron - Contrat Plan Etat Région Poitou-Charentes 1999 - Rapport 1999
Provedor de dados: 5
Autores: Lapegue, Sylvie; Thiriot Quievreux, Catherine; Mccombie, Helen; Heurtebise, Serge; Boudry, Pierre; Robert, Stephane; Soletchnik, Patrick; Goulletquer, Philippe; Gerard, Andre.
Divers travaux, menés depuis 1984, montrent qu'il existe, chez l'huître creuse Crassotrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Le but de l'expérimentation menée en 1999 est de faire un...
Tipo: Text
Palavras-chave: Génétique; Aneuploïdie; Génétique des populations; Biochimie; Marennes-Oléron; Huître creuse; Crassostrea gigas.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00044/15522/12909.pdf

Chromosome loss in tetraploid families of the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre.
Alterations of  chromosome number have been observed in the somatic tissue of  Crassostrea gigas diploids and artificial polyploids bred in this species.   Tetraploid animals with lower chromosome counts in some or all of their somatic tissue are considered undesirable as parents either for commercial triploids (produced via a  cross with diploids) or for tetraploid breeding because (1) they may confer the tendency to lose chromosomes and revert to lower ploidy levels to their offspring or (2) their offspring could have a lower ploidy directly because of potential links between somatic and gametic chromosome loss.  The present study evaluated the phenomenon in bi-parental tetraploid families bred from parents of differing ploidy quality, including a...
Tipo: Text
Palavras-chave: Tetraploid; Chromosome; Genetic; Crassostrea gigas; Oyster.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3435.pdf

Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Boudry, Pierre; Beaumont, Andy R.; Lapegue, Sylvie.
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
Tipo: Text
Palavras-chave: Universal tailed primer labelling; Oysters; Ostrea edulis; Microsatellites.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6421.pdf

Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus
Provedor de dados: 5
Autores: Huvet, Arnaud; Fabioux, Caroline; Mccombie, Helen; Lapegue, Sylvie; Boudry, Pierre.
The marine environment is of special interest for studying hybridization between closely related taxa because of the high dispersal potential of planktonic larvae, such as those of most bivalve species. The oysters Crassostrea angulata and C. gigas are known to be very close genetically and entirely inter-fertile under controlled conditions. However, hybridization in the wild had not been investigated, mainly due to the lack of nuclear diagnostic markers. In the present paper, we first estimated genetic differentiation between these 2 closely related taxa using 8 microsatellite markers. Interestingly, 5 markers displayed significant differences of allele size between taxa. The subsequent sequencing of alleles of one of these microsatellites showed several...
Tipo: Text
Palavras-chave: Allele size; Microsatellites; Genetic differentiation; Hybridization; Crassostrea gigas; Crassostrea angulata.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-3355.pdf

Combined functional genomics and genetic approach in oyster to identify summer mortality-resistant markers
Provedor de dados: 5
Autores: Huvet, Arnaud; Lapegue, Sylvie.
The oyster Crassostrea gigas has global distribution and for the past several years the highest annual production of any freshwater or marine organism. Economic importance of oysters motivates a great deal of biological research, which provides the most immediate scientific rationales for sequencing ESTs and developing genomic tools.
Tipo: Text
Palavras-chave: Environmental genomics; Comparative genomics; Functional genomics; Markers; Summer mortalities; Genetic; Oysters; Crassostrea gigas.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4006.PDF

Programme 3 : Gestion durable des productions ostréicoles : les apports de la génétique. Convention 2000 RPC-A-530 Ifremer - Région Poitou-Charentes
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Heurtebise, Serge; Ledu, Christophe; Blouin, Frederic; Phelipot, Pascal; Gerard, Andre.
L'étude de la variabilité génétique de l' huître creuse Crassastrea gigas est un point essentiel dans la mise en place de programmes de gestion des ressources génétiques et de sélection chez cette espèce. C. gigas, originaire du Japon, a été introduite dans de nombreuses régions du monde et représente aujourd'hui plus de 95 % de la production mondiale d' huîtres. Introduite en France au début des années 1970 pour remplacer l' huître portugaise Crassostrea angulata, c'est aujourd 'hui une ressource de première importance pour notre région. Malgré la maîtrise du cycle de reproduction de l'espèce et son importance commerciale, elle n' a pas été génétiquement améliorée et on peut donc considérer les stocks comme « sauvages» ou « non domestiqués ». De plus, la...
Tipo: Text
Palavras-chave: Génétique; Ressources génétiques; Différenciation génétique; Hybridation; Anomalies chromosomiques; Huîtres creuses; Crassostrea gigas; Crassostrea angulata.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15564/12955.pdf

Caractérisation des facteurs de risques associés au mortalités estivales - Synthèse du thème 3 - Risque associé au stress environnemental - 2002-2005
Provedor de dados: 5
Autores: Burgeot, Thierry; Gagnaire, Beatrice; Renault, Tristan; Haure, Joel; Moraga, D.; David, E.; Boutet, L.; Sauriau, P.g.; Malet, Nathalie; Bouchet, V.; Lapegue, Sylvie; Bouilly, Karine; Le Moullac, Gilles; Arzul, Genevieve; Knoery, Joel; Quiniou, Francoise; Bacher, Cedric; Soletchnik, Patrick.
Les mortalités d'huîtres observées sur les côtes françaises apparaissent principalement à proximité du sédiment et pendant la gamétogenèse. Les interactions de facteurs environnementaux sur les écosystèmes conchylicoles comme la température, les apports des bassins versants et le compartiment sédimentaire illustrent un risque de mortalité lié à différentes sources de stress (Thème 1). Les variations de la qualité de la ressource trophique pendant l'effort de gamétogenèse favorise une baisse de l'énergie disponible (Thème 2) et contraint l'organisme fragilisé à fournir un effort d'adaptation (Fent 2004). L'apparition d'un stress pendant cette période sensible des huîtres pourrait constituer un facteur aggravant de mortalité. L'objectif de cette étude est...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00304/41538/40746.pdf

Microsatellites development in Ostrea edulis and Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Lapegue, Sylvie; Boudry, Pierre; Beaumont, Andy.
The European flat oyster Ostrea edulis and the blue mussel Mytilus edulis are both very valuable commercial species across Europe. Despite their economical importance, only a few microsatellite markers have so far been developed in those species. The aim of this study was to develop new microsatellites for O. edulis and M. edulis that could be used for population genetics studies, genetic variability assessment of stocks, parentage analysis or genetic and QTL mapping studies. For Ostrea edulis, an enriched library was made by ecogenics GmbH (Zurich, Switzerland) from size selected genomic DNA ligated into SAULA/SAULB-linker and enriched by magnetic bead selection with biotin-labelled (GT)13 and (CT)13 oligonucleotide repeats (Gautschi et al. 2000a,b). Of...
Tipo: Text
Palavras-chave: M13 tail; Blue mussel; European flat oyster; Microsatellite.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4536.pdf