Consulta: creatorFacets:"Leiva,LC"
Registros recuperados: 3
Data/hora: 09/06/2024 01:21:21

IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: cross-reaction with venoms from Bothrops species from Argentina
Provedor de dados: 80
Autores: Rodríguez,JP; De Marzi,MC; Maruñak,S; Teibler,P; Acosta,O; Malchiodi,EL; Leiva,LC.
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops; Crotalus; PLA2; Neutralization; Cross-reaction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300008

Cross-neutralization of the coagulant activity of Crotalus durissus terrificus venom from the northeast of Argentina by bivalent bothropic antivenom
Provedor de dados: 80
Autores: Rodríguez,JP; Gay,CC; Fusco,LS; Gauna,MC; Acosta,OC; Leiva,LC.
Cross-neutralization of Crotalus durissus terrificus venom coagulant activity was tested using bivalent horse antivenom against Bothrops alternatus and Bothrops diporus venoms. Our in vitro and in vivo experiments showed that bothropic antivenom neutralizes the thrombin-like activity of crotalic snake venom and this cross-reaction was demonstrated by immunoassays either with whole venom or a purified thrombin-like enzyme. These results suggest common antigenic properties and, consequently, similar molecular structure among venom thrombin-like enzymes. Besides, they provide information that could be further used in the development of new antivenom formulations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thrombin-like enzyme; Snake venom; Antivenoms; Immunological cross-reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100015

Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
Provedor de dados: 80
Autores: Bustillo,S; Lucero,H; Leiva,LC; Acosta,O; Kier Joffé,EB; Gorodner,JO.
Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 µg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50: 2 µg/mL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops alternatus; Bothrops diporus; Cytotoxicity; C2C12; Apoptosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100004