Consulta: creatorFacets:"Lilenbaum,Walter"
Registros recuperados: 19
Data/hora: 02/06/2024 06:30:40

Improvement of an in-house ELISA for bovine paratuberculosis serology in Brazil
Provedor de dados: 58
Autores: Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R.
Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of bacilli at levels detectable by fecal culture is irregular and does not occur during the early stages of infection, which compromises the sensitivity of this methodology. The most common immunological tests to identify Map infection are complement fixation test (CFT), agar gel immunodiffusion (AGID) and enzyme-linked immunoassay (ELISA). In Brazil,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Cattle; ELISA; Serology; Diagnosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200004

Comparison of serological methods for the diagnostic of Caprine arthritis-encephalitis (CAE) in Rio de Janeiro, Brazil
Provedor de dados: 58
Autores: Cortez-Moreira,Madelayne; Oelemann,Walter M.R.; Lilenbaum,Walter.
Serum samples of 562 goats were tested for Caprine arthritis-encephalitis using agar gel immunodiffusion test (AGID) and enzyme linked immunosorbent assay (ELISA). Seventy-nine samples (14.1%) were reactive by both testes but 141 (25.1%) were reactive only by ELISA. ELISA, when compared to AGID, presented 100% sensitivity and 70.8% specificity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprine arthritis-encephalitis; CAE; Goats; ELISA; AGID.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100010

Potential application of new diagnostic methods for controlling bovine Tuberculosis in Brazil
Provedor de dados: 58
Autores: Medeiros,Luciana dos Santos; Marassi,Carla Dray; Figueiredo,Eduardo Eustáquio S.; Lilenbaum,Walter.
Bovine tuberculosis, a chronic infection in cattle caused by Mycobacterium bovis, remains an economic and public health problem for several countries. Due to its economic impact on international trade, contagious nature, and implications for human health, global programs to eradicate the disease were implemented worldwide. Those programs are based on slaughtering PPD-reactive animals. Despite the National Programs in Brazil, complete eradication has not been achieved, and the disease remains, albeit at a lower prevalence. The central purpose of this review is to address diagnostic tests for tuberculosis. Considering the course of the infection in cattle, at least two tests, ideally complementary to one another, may be necessary for an adequate diagnosis:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine Tuberculosis; Mycobacterium bovis; Diagnosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300002

Molecular characterization of the first leptospires isolated from goats in Brazil
Provedor de dados: 58
Autores: Lilenbaum,Walter; Kremer,Frederico; Ristow,Paula; Dellagostin,Odir; Bourhy,Pascale; Hartskeerl,Rudy; Vasconcellos,Silvio.
Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Serogrouping; Sequencing; PFGE; Rrs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050

Identification of Mycobacterium bovis Isolates by a multiplex PCR
Provedor de dados: 58
Autores: Figueiredo,Eduardo Eustáquio de Souza; Silvestre,Flávia Galindo; Campos,Wilma Neres; Furlanetto,Leone Vinícius; Medeiros,Luciana; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Bovine tuberculosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200004

An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
Provedor de dados: 58
Autores: Marassi,Carla Dray; Silva,Marley; Oelemann,Walter Martin Roland; Fonseca,Leila de Souza; Ristow,Paula; Lilenbaum,Walter.
ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ &gt; 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacteria; ELISA; M. fortuitum; M. phlei.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019

Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
Provedor de dados: 58
Autores: Marassi,Carla Dray; Gonzaga,Janete da Silva; Ristow,Paula; Ferreira,Rachel; Fonseca,Leila Souza; Oelemann,Walter; Lilenbaum,Walter.
An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (kappa&gt; 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Mycobacterium avium subsp. paratuberculosis; Serology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100002

Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
Provedor de dados: 58
Autores: Lilenbaum,Walter; Ferreira,Rachel; Marassi,Carla Dray; Ristow,Paula; Oelemann,Walter Martins Roland; Fonseca,Leila de Souza.
Forty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative animals from infected herds, and Group C with 21 TB-negative cows from a tuberculosis-free herd (which were used as controls). Twenty of 28 animals from Group A (71.4%), 6/16 from Group B (37.5%) and none from Group C were reactive to PTB ELISAs, suggesting that these commercial kits were unable to distinguish between PTB and TB. It is proposed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Tuberculosis; Mycobacterium bovis; Mycobacterium avium paratuberculosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300016

First isolation of leptospires from dairy goats in Brazil
Provedor de dados: 58
Autores: Lilenbaum,Walter; Morais,Zenaide M.; Gonçales,Amane Paldês; Souza,Gisele O. de; Richtzenhain,Leonardo; Vasconcellos,Silvio A.
Leptospires have never been recovered from goats in Brazil. Serum samples were obtained from 248 goats from Rio de Janeiro and from the seroreactive animals, urine samples were collected and processed for Leptospira isolation. A total of 52 positive reactions were observed, corresponding to 20.9% of the samples. The most prevalent reactions were to serovars Hardjo (36.5%), Shermani (30.8%), Icterohaemorrhagiae (9.6%), Grippotyphosa (9.6%), Autumnalis (5.8%), Castellonis (3.8%) and Bratislava (3.8%). Two strains of Leptospira sp. were isolated, both in the same region, but from different flocks. Presumptive identification based on serologic methods suggests those strains to be from Grippotyphosa serogroup.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goats; Leptospirosis; Leptospira sp.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300023

Comparison of cefoxitin disk diffusion test and mecA gene PCR results for methicillin resistance detection in Staphylococcus intermedius group isolates from canine origin in Brazil
Provedor de dados: 58
Autores: Penna,Bruno; F Rabello,Renata; Lilenbaum,Walter.
The study evaluated cefoxitin disk diffusion tests breakpoints and their correlation to mecA gene PCR results for detecting Methicillin-resistant Staphylococcus intermedius Group (MRSP) isolates from dogs in Brazil. Agreement using proposed breakpoint (resistant &lt; 30 mm) was encouraging. The current study reinforces that an epidemiological breakpoint can be established to predict presence of MRSP.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methicillin-resistant Staphylococcus intermedius group; Cefoxitin; Disk diffusion test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100033

Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
Provedor de dados: 58
Autores: Figueiredo,Eduardo Eustáquio de Souza; Carvalho,Ricardo Cezar Tavares; Silvestre,Flávia Galindo; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi.
Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Nasal swab; Bovine tuberculosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020

Molecular demonstration of intermittent shedding of Leptospira in cattle and sheep and its implications on control
Provedor de dados: 65
Autores: Rocha,Bruno Ribeiro; Narduche,Lorena; Oliveira,Clara Slade; Martins,Gabriel; Lilenbaum,Walter.
ABSTRACT: For a long time, it has been stated that urine leptospiral shedding is intermittent, which was observed primarily by culturing. However, culturing presents serious limitations, mainly low sensitivity, and failure on detection of leptospires cannot be neglected. PCR presents several advantages, mainly higher sensitivity. The present study aimed to analyze the occurrence of intermittency on leptospiral shedding by PCR in naturally and experimentally infected animals. In this study two experiments were conducted, the first with 60 cows naturally infected from an endemic herd. The second one was conducted in three sheep experimentally infected, each one with a different strain of Leptospira (strains Copenhageni L1-130, Canicola LO-4 and Pomona...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cows; Intermittent; PCR; Sheep; Strain.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800453

Qualitative and quantitative analysis of bacteria from vaginitis associated with intravaginal implants in ewes following estrus synchronization
Provedor de dados: 65
Autores: Vasconcelos,Carlos Otávio de Paula; Brandão,Felipe Zandonadi; Martins,Gabriel; Penna,Bruno; Souza-Fabjan,Joanna Maria Gonçalves de; Lilenbaum,Walter.
ABSTRACT: This study evaluated the presence of vaginitis and the bacterial load associated with different intravaginal implants in ewes. Twenty-four Dorper and crossbred ewes were allocated into three groups and received intravaginal implant containing 0.3g progesterone (CIDR(r)), 60mg MAP or sponges without progesterone (CONTROL) for six days. Then, CIDR and MAP treated-ewes received 12.5mg dinoprost and 300IU eCG. Vaginal mucus samples were collected at four times: before device insertion, at the day of its removal, 24 and 48 hours after. The samples were cultured and the colonies were counted (CFU/mL) and identified. The results obtained from the counting of CFU mL-1 were submitted to Kruskal-Wallis H test, with P&lt;0.05 being considered significant....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intravaginal device; Microorganism; Sheep; Vaginal infection.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400632

Leptospirosis in native mixed-breed sheep slaughtered in a semiarid region of Brazil
Provedor de dados: 65
Autores: Costa,Diego Figueiredo da; Silva,Aline Ferreira da; Brasil,Arthur Willian de Lima; Loureiro,Ana Paula Pereira; Santos,Fabrine Alexandre dos; Azevedo,Sergio Santos de; Lilenbaum,Walter; Alves,Clebert José.
ABSTRACT: Sheep breeding is an economic activity of great value for the Brazilian livestock industry, and infectious diseases can significantly compromise its productivity. The aim of this study was to detect Leptospira spp. in mixed breed sheep from a semiarid region of the State of Paraíba, Northeast Brazil. In total, 49 blood and renal tissue samples, as well as 25 urine samples, were collected from 49 sheep in the slaughter line. The microscopic agglutination test (MAT) was used to verify the presence of anti -Leptospira spp. antibodies in blood samples. Renal tissue and urine samples were analyzed by both PCR and bacterial culture. Sero reactivity was observed in 8.2% (4/49) of animals. Three renal samples were positive for leptospiral DNA by PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Small ruminants; PCR; Serology; Isolation; Slaughterhouse.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200451

Níveis séricos de uréia e creatinina em cães com leptospirose aguda determinada por amostras do sorogrupo Icterohaemorrhagiae
Provedor de dados: 65
Autores: Freire,Isabel Maria Alexandre; Varges,Renato; Lilenbaum,Walter.
Foram testadas 120 amostras séricas de cães com títulos (&lt;FONT FACE=Symbol&gt;³&lt;/FONT&gt;100) perante Icterohaemorrhagiae para a determinação dos níveis séricos de uréia e de creatinina. Os animais com títulos &lt;FONT FACE=Symbol&gt;³&lt;/FONT&gt;800 apresentaram dosagens de uréia bastante superiores aos valores de referência e os com títulos &lt;FONT FACE=Symbol&gt;³&lt;/FONT&gt;200 e &lt;FONT FACE=Symbol&gt;£&lt;/FONT&gt;400 apresentaram valores de uréia significativamente menores (P&lt;0,05) do que os anteriores, porém ainda acima dos valores de referência. As amostras com títulos menores que 200 apresentaram dosagens de uréia significativamente menores (P&lt;0,05) do que os observados nos outros grupos e dentro dos valores de referência. No que...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirose; Cães; Uréia; Creatinina; Icterohaemorrhagiae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400045

Alterações na bioquímica hepática em cães com leptospirose aguda determinada por amostras do sorogrupo Icterohaemorrhagiae
Provedor de dados: 65
Autores: Freire,Isabel Maria Alexandre; Varges,Renato; Lilenbaum,Walter.
Foram testadas 120 amostras séricas de cães com títulos (&lt;FONT FACE=Symbol&gt;³&lt;/FONT&gt;100) perante Leptospira spp. serovar Icterohaemorrhagiae para a determinação da atividade sérica de alanina aminotransferase (ALT), aspartato aminotransferase (AST), fosfatase alcalina (FA), gamaglutamiltransferase (GGT) e dos níveis séricos de colesterol total, triglicerídeos, proteína total, albumina, globulinas e bilirrubinas total, direta e indireta. Um grupo de 34 animais sem qualquer sintomatologia clínica e idade semelhante ao grupo testado foi utilizado como controle, tendo sido submetido às mesmas dosagens bioquímicas. Os animais com títulos &lt;FONT FACE=Symbol&gt;³&lt;/FONT&gt;200 apresentaram dosagens de FA e bilirubina direta superiores ao grupo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirose; Cães; Icterohaemorrhagiae; Fígado.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000900037

Seropositivity to Leptospira interrogans serovar Bratislava associated to reproductive problems without significant biochemical or hematological alterations in horses
Provedor de dados: 65
Autores: Pinna,Melissa; Martins,Gabriel; Freire,Isabel; Lilenbaum,Walter.
The objective was to study haematological and biochemical alterations associated to seropositivity to Leptospira interrogans serovar Bratislava infection in horses with reproductive alterations, such as neonatal deaths, embryonic deaths and abortions. A flock of mares with poor reproductive performance was studied. Eighty-two (58.6%) were seropositive (titre 200); 72 of those (87.8%) for Bratislava. Slight haematological and biochemical alterations were observed, being more frequent (P&lt;0.05) in horses seropositive versus seronegative. In conclusion horses seropositive for Bratislava had no consistent, severe alteration in values for hematology and biochemistry serum. Those findings reinforce this serovar as adapted to the horse and causing only mild...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Horse; Bratislava; Hematology; Biochemistry.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001000027

Seroprevalence of Leptospira spp. infection in sheep from northwest of Rio Grande do Sul, Brazil
Provedor de dados: 65
Autores: Consalter,Angélica; Zanella,Eraldo Lourenso; Martins,Gabriel Mendes de Souza; Araujo,Hugo Libonati de; Lilenbaum,Walter; Souza,Guilherme Nunes de; Ferreira,Ana Maria Reis.
ABSTRACT: Leptospirosis is a zoonosis that is widely distributed in tropical countries. This infection is also associated with reproductive losses in livestock, which has a significant economic impact. The objective of this study is to investigate the seroprevalence and risk factors associated with leptospiral infection in commercial sheep flocks from the northwestern mesoregion of Rio Grande do Sul. Serum samples were analyzed in 319 sheep using the Microscopic Agglutination Test (MAT). At the time of sampling, an individual and herd questionnaire was carried out to investigate the risk factors. On an individual level, positive reactions were observed in 5.6% (18/319) of the studied sera, with Sejroe being the most prevalent serogroup. Animals &gt;3 years...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira spp.; Sheep; MAT; Seroprevalence.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200451

Repetition of estrus is the most frequent reproductive problem after breeding in dairy cattle from Rio de Janeiro, Brazil
Provedor de dados: 65
Autores: Arashiro,Eduardo Kenji Nunes; Libonati,Hugo; Santos,Gustavo Bervian dos; Nogueira,Luiz Altamiro Garcia; Souza,Guilherme Nunes de; Lilenbaum,Walter; Brandão,Felipe Zandonadi.
ABSTRACT: Reproductive efficiency is one of the key elements for any milk production system to be successful. In this regard, reproductive management must be planned properly and the identification of the main reproductive disorders that affect the herd is primordial for decision-making. The aim of the present study was to identify the main reproductive problems that affect dairy herds on Rio de Janeiro (RJ), Brazil. Data regarding breed, age, reproductive disorder occurrence, and breeding system were individually collected from dairy cows raised at 25 farms located at RJ. The occurrence of reproductive disorders was reported in 37% of the studied animals. Estrous repetition (76.2%) and abortion (15.6%) were the most frequent reproductive problems...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cattle; Reproductive disorders; Repeat breeding.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700701