Consulta: creatorFacets:"Linhares,Rosa Elisa Carvalho"
Registros recuperados: 4
Data/hora: 13/06/2024 06:33:55

Incidence of group A rotavirus in urban and rural areas of the city of Londrina-Brazil, from 1995 to 1997
Provedor de dados: 52
Autores: Meneghetti,Ângelo Cesar; Bolognini,Andrea Maria; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Santos,Norma; Nozawa,Carlos Mitihiko.
Rotaviruses are common pathogens and the causal agents of acute diarrhea among children and young animals. The involvement of rotavirus in human diarrheal disease among population of urban and rural areas of the city of Londrina, Parana was evaluated. Nine hundred and five fecal specimens from persons with diarrhea were studied, being 686 and 219 from urban and rural areas, respectively. Thirty-eight samples (4,2%) were positive for rotavirus by polyacrylamide gel electrophoresis of viral RNA and latex agglutination test of which 36 were from urban and two from rural areas. Out of the positive specimens, 17 strains were further characterized by RT-PCR typing assay, resulting in 16 strains of G1 genotype while one sample was found to be a mixture of G1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Diarrhea; PAGE; LA; PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300006

Development of an enzyme immunoassay for poliovirus antigens
Provedor de dados: 52
Autores: Hashimoto,Newton; Benati,Fabrício José; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Nozawa,Carlos Mitihiko.
An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme immunoassay; Poliovirus antigen.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005

Detection and characterization of human rotavirus in hospitalized patients in the cities of Ponta Grossa, Londrina and Assai - PR, Brazil
Provedor de dados: 55
Autores: Nozawa,Carlos M; Kerntopf,Gustavo Franthesco; Czernisz,Érika da Silva; Albuquerque,Daniele; Romanin,Priscila; Freitas,José Felipe Eliseu; Santos,Norma; Benati,Fabrício José; Pietruchinski,Eduardo; Linhares,Rosa Elisa Carvalho.
Acute diarrheal disease is still one of the major public health problems worldwide. Rotaviruses (RV) are the most important viral etiologic agents and children under five years of age are the target population. OBJECTIVE: To investigate the rate of RV infection in hospitalized patients due to acute diarrhea in the cities of Ponta Grossa, Londrina and Assai - Paraná. METHODS: Latex agglutination (LA); immunochromatography (ICG); polyacrylamide gel electrophoresis (PAGE) and negative staining electron microscopy (ME) tests were used to detect the virus. For the genotyping, RT-PCR and RT-PCR-ELISA were used, respectively, for NSP4 and VP4/VP7. RESULT: Out of 124 samples there were 69 positive stool samples for RV, for at least one of the used tests, 67 of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Determination; Hospitalized patients; Genotyping.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600002

Antiviral activity of Agaricus blazei Murrill ss. Heinem extract against human and bovine herpesviruses in cell culture
Provedor de dados: 58
Autores: Bruggemann,Rafaela; Matsuo Orlandi,Janaina; Benati,Fabricio Jose; Faccin,Ligia Carla; Mantovani,Mario Sergio; Nozawa,Carlos; Linhares,Rosa Elisa Carvalho.
The aqueous extract of Agaricus blazei Murill ss. Heinem, a basidiomycete native from Brazil, frequently used by popular medicine, mainly in the form of tea, was assessed to its antiviral action against herpes simplex type 1 (HSV-1) and bovine herpes type 1 (BoHV-1) in HEp-2 cell culture. Viral replication inhibition was evaluated by plaque assay and immunofluorescence test. The extract demonstrated virucide action for both viruses, being more effective against HSV-1, inhibiting its infectivity in 78.4 and 73.9% at the concentrations of 50 and 100 µg/mL, respectively moreover, reduction in 47% the number of fluorescent cells was observed for both concentrations. The extract also showed discrete therapeutic activity. These results suggest that A. blazei...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei; BoHV-1; HSV-1; Antiviral activity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400029