Consulta: creatorFacets:"Liu,Ying"
Registros recuperados: 10
Data/hora: 09/06/2024 13:51:40

Genome-wide identification and characterization of R2R3-MYB genes in Medicago truncatula
Provedor de dados: 74
Autores: Li,Wei; Liu,Ying; Zhao,Jinyue; Zhen,Xin; Guo,Changhong; Shu,Yongjun.
Abstract MYB is a large family of plant transcription factors. Its function has been identified in several plants, while there are few reports in Medicago truncatula. In this study, we used RNA-seq data to analyze and identify R2R3-MYB genes in the genome of Medicago truncatula. Phylogenetic analysis classified 150 MtMYB genes into 21 subfamilies with homologs. Out of the 150 MtMYB genes, 139 were distributed among 8 chromosomes, with tandem duplications (TD) and segment duplications (SD). Microarray data were used for functional analysis of the MtMYB genes during growth and developmental processes providing evidence for a role in tissues differentiation, seed development processes, and especially the nodulation process. Furthermore, we investigated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicago truncatula; MYB; Abiotic stress; QRT-PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400611

Transcriptome sequencing and expression profiling of genes involved in the response to abiotic stress in Medicago ruthenica
Provedor de dados: 74
Autores: Shu,Yongjun; Li,Wei; Zhao,Jinyue; Liu,Ying; Guo,Changhong.
Abstract Medicago ruthenica is a perennial forage legume with the remarkable ability to survive under unfavorable environmental conditions. It has been identified as an excellent species of Medicago that can adapt to various environmental stresses including low temperature, drought, and salinity. To investigate its potential as a genetic resource, we performed transcriptome sequencing and analysis in M. ruthenica under abiotic stresses. We generated &gt;120 million reads from six cDNA libraries, resulting in 79,249 unique transcripts, most of which were highly similar to transcripts from M. truncatula (44,608, 56.3%) and alfalfa (M. sativa, 48,023, 60.6%). Based on gene expression profiles, 2,721 transcripts were identified as abiotic stress responsive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicago ruthenica; RNA-Seq; Transcription factor (TF); Abiotic stress; Reactive oxygen species (ROS).
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400638

Transcriptome sequencing analysis of alfalfa reveals CBF genes potentially playing important roles in response to freezing stress
Provedor de dados: 74
Autores: Shu,Yongjun; Li,Wei; Zhao,Jinyue; Zhang,Sijia; Xu,Hanyun; Liu,Ying; Guo,Changhong.
Abstract Alfalfa (Medicago sativa L.) is an important perennial forage, with high nutritional value, which is widely grown in the world. Because of low freezing tolerance, its distribution and production are threatened and limited by winter weather. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed transcriptome sequencing analysis under cold (4 °C) and freezing (-8 °C) stresses. More than 66 million reads were generated, and we identified 5767 transcripts differentially expressed in response to cold and/or freezing stresses. These results showed that these genes were mainly classified as response to stress, transcription regulation, hormone signaling pathway, antioxidant, nodule morphogenesis, etc., implying...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicago sativa L.; RNA-seq; Freezing tolerance; C-repeat binding factors (CBF).
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500824

The association of HLA-DRB1 alleles and drug use with HIV infection in a Chinese Han Cohort
Provedor de dados: 55
Autores: Diao,Bo; Du,Juan; Liu,Ying; Luo,Fan; Hou,Wei.
Objective:To investigate the relationship between the polymorphism of human leukocyte antigen (HLA)-DRB1 and the susceptibility and repellency of drug use combined with HIV infection in Chinese.Methods:A total of 213 unrelated healthy people, 41 HIV-infected drug users, 24 HIV-uninfected drug users, and 64 HIV-infected non-drug users were recruited. Their HLA-DRB1 allele frequencies were analyzed by PCR-SSP and allele distribution was analyzed.Results:Compared with healthy controls, in drug users, the frequencies of HLA-DRB1 *0401-041, *1001 were significantly higher; in HIV-infected patients, the frequencies of HLA-DRB1 *0101-0103, *0401-0411, *1001 were significantly higher, while the frequencies of DRB1 *1501-1502, *1101-1105, *1301-1302, DRB4, DRB5...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: HLA-DRB1; Polymorphism; Drug use combined with HIV; Infection; Frequency.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100082

Critical role of metabotropic glutamate receptor 4 in bone marrow-derived dendritic cells in the Th17 cell differentiation and the melanogenesis of B16 cells
Provedor de dados: 56
Autores: Zhao,Guangming; Zhou,Wenhui; Liu,Ying; Wang,Yupeng; Li,Zhou; Song,Zhiqi.
Vitiligo is an acquired pigmentary disorder resulting from selective destruction of melanocytes. Emerging studies have suggested that T helper cell 17 (Th17) is potentially implicated in vitiligo development and progression. It was recently discovered that metabotropic glutamate receptor 4 (mGluR4) can modulate Th17-mediated adaptive immunity. However, the influence of mGluR4 on melanogenesis of melanocytes has yet to be elucidated. In the present study, we primarily cultured mouse bone marrow-derived dendritic cells (BMDC) and then knocked down and over-expressed mGluR4 using transfection. Transduced BMDC were co-cultured with CD4+ T cells and the expression of Th17-related cytokines were measured. The morphology and melanogenesis of B16 cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabotropic glutamate receptor-4; Th17; Melanogenesis; Vitiligo.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400604

[RETRACTED ARTICLE] Ginsenoside Rd inhibits IL-1β-induced inflammation and degradation of intervertebral disc chondrocytes by increasing IL1RAP ubiquitination
Provedor de dados: 56
Autores: Zhang,Wei-Jia; Liu,Ying; Wei,Jie-Shu; Wu,Ya-Li.
Many compounds of ginsenosides show anti-inflammatory properties. However, their anti-inflammatory effects in intervertebral chondrocytes in the presence of inflammatory factors have never been shown. Increased levels of pro-inflammatory cytokines are generally associated with the degradation and death of chondrocytes; therefore, finding an effective and nontoxic substance that attenuates the inflammation is worthwhile. In this study, chondrocytes were isolated from the nucleus pulposus tissues, and the cells were treated with ginsenoside compounds and IL-1β, alone and in combination. Cell viability and death rate were assessed by CCK-8 and flow cytometry methods, respectively. PCR, western blot, and immunoprecipitation assays were performed to determine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginsenoside Rd; IL-1β; Inflammation; Degradation; Intervertebral disc chondrocytes; IL1RAP ubiquitination.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900602

Interleukin-6 contributes to myocardial damage in pregnant rats with reduced uterine perfusion pressure
Provedor de dados: 56
Autores: Ding,Lan; Bai,Chuanming; Liu,Ying.
Preeclampsia is one of the most frequent and difficult illnesses in pregnancy, which jeopardizes both mother and fetus. There are several diagnostic criteria for preeclampsia. However, the preeclampsia-associated myocardial damage has not been described. In this study, we employed reduced uterine perfusion pressure (RUPP) to generate a rat model of preeclampsia for the evaluation of myocardial damage in late-gestation rats. The expressions of cardiac injury markers were analyzed by immunohistochemistry and ELISA. The arterial pressure and myocardial tissue velocities were also measured. The role of interleukin (IL)-6 in RUPP-associated myocardial damage was further explored. The results showed that RUPP rats had significant myocardial damage, as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Myocardial damage; Reduced uterine perfusion pressure; Interleukin-6.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800608

Observation of the effect of bone marrow mesenchymal stem cell transplantation by different interventions on cirrhotic rats
Provedor de dados: 56
Autores: Zhou,Xiaoling; Yang,Jianqing; Liu,Ying; Li,Zepeng; Yu,Jingfang; Wei,Wanhua; Chen,Qiao; Li,Can; Tang,Nong.
Bone marrow mesenchymal stem cells (BMSCs) transplantation has attracted attention for the treatment of liver cirrhosis and end-stage liver diseases. Therefore, in this study, we evaluated the effect of different methods of BMSCs transplantation in the treatment of liver cirrhosis in rats. Seventy-two male Sprague-Dawley rats were divided into 7 groups: 10 were used to extract BMSCs, 10 were used as normal group, and the remaining 52 rats were randomly divided into five groups for testing: control group, BMSCs group, BMSCs+granulocyte colony-stimulating factor (G-CSF) group, and BMSCs+Jisheng Shenqi decoction (JSSQ) group. After the end of the intervention course, liver tissue sections of rats were subjected to hematoxylin and eosin (H&amp;E) and Masson...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow mesenchymal stem cells; Transplantation; Liver cirrhosis; Granulocyte colony-stimulating factor; Jisheng Shenqi decoction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300606

Cloning of a novel xylanase gene from a newly isolated Fusarium sp. Q7-31 and its expression in Escherichia coli
Provedor de dados: 58
Autores: Xie,Zhan-Ling; Gao,Hai-Yan; Zhang,Qian; Wang,Huan; Liu,Ying.
A strain of Q7-31 was isolated from Qinghai-Tibet Plateau and was identified as Fusarium sp. based on its morphological characteristics and ITS rDNA gene sequence analysis. It has the highest capacity of degrading cell wall activity compared with other 11 strains. To do research on its xylanase activity of Fusarium sp. Q7-31 while the degrading the rice cell walls, the complete gene xyn8 that encodes endo-1, 4-β-xylanase secreted by Fusarium sp. Q7-31 was cloned and sequenced. The coding region of the gene is separated by two introns of 56bp and 55bp. It encodes 230 amino acid residues of a protein with a calculated molecular weight of 25.7 kDa. The animo acids sequence of xyn8 gene has higher similarity with those of family 11 of glycosyl hydrolases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylanase; Gene cloning; Fusariumsp.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100049

Antimicrobial susceptibility and fluctuations in clonal complexes of serogroup 6 Streptococcus pneumoniae isolates collected from children in Beijing, China, between 1997 and 2016
Provedor de dados: 58
Autores: Shi,Wei; Liu,Ying; Meng,Qinghong; Yuan,Lin; Gao,Wei; Yao,Kaihu.
ABSTRACT This study examined the antimicrobial susceptibility patterns and clonal complex (CC) characteristics of serogroup 6 Streptococcus pneumoniae isolates collected from children in Beijing, China, between 1997 and 2016. Serotypes were determined using the Quellung reaction, and the antimicrobial susceptibility profiles of the isolates were determined using the disc-diffusion method or by E-test. Sequence types (STs) were assigned based on multilocus sequence typing. A total of 250 isolates were examined, with 55.2%, 30.0%, 12.8%, and 2.0% of isolates identified as serotypes 6A, 6B, 6C, and 6D, respectively. All of the isolates were susceptible to levofloxacin and vancomycin, and the non-suceptibitility rate to penicillin was 41.6%. Eighty-two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Children; Antimicrobial susceptibility; Clonal complex (CC); Sequence type (ST).
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400891